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Protein

Carbohydrate sulfotransferase 7

Gene

Chst7

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues. Preferentially acts on mannose-linked GlcNAc. Also able to catalyze the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Also acts on core 2 mucin-type oligosaccharide and N-acetyllactosamine oligomer with a lower efficiency. Has weak or no activity toward keratan sulfate and oligosaccharides containing the Galbeta1-4GlcNAc. Catalyzes 6-O-sulfation of beta-benzyl GlcNAc but not alpha- or beta-benzyl GalNAc (By similarity).By similarity

Catalytic activityi

3'-phosphoadenylyl sulfate + chondroitin = adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi109 – 115PAPSBy similarity7
Nucleotide bindingi277 – 285PAPSBy similarity9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

ReactomeiR-RNO-2022870. Chondroitin sulfate biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbohydrate sulfotransferase 7 (EC:2.8.2.-, EC:2.8.2.17)
Alternative name(s):
Chondroitin 6-sulfotransferase 2
Short name:
C6ST-2
Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 5
Short name:
GST-5
N-acetylglucosamine 6-O-sulfotransferase 4
Short name:
GlcNAc6ST-4
Short name:
Gn6st-4
Gene namesi
Name:Chst7
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome X

Organism-specific databases

RGDi1303028. Chst7.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 12CytoplasmicSequence analysisAdd BLAST12
Transmembranei13 – 33Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini34 – 485LumenalSequence analysisAdd BLAST452

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000852001 – 485Carbohydrate sulfotransferase 7Add BLAST485

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi88N-linked (GlcNAc...)Sequence analysis1
Glycosylationi185N-linked (GlcNAc...)Sequence analysis1
Glycosylationi406N-linked (GlcNAc...)Sequence analysis1
Modified residuei461PhosphoserineBy similarity1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ6XQG8.
PRIDEiQ6XQG8.

Expressioni

Gene expression databases

BgeeiENSRNOG00000004258.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000005630.

Structurei

3D structure databases

ProteinModelPortaliQ6XQG8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IIA6. Eukaryota.
ENOG4111DKG. LUCA.
GeneTreeiENSGT00530000062902.
HOGENOMiHOG000261614.
HOVERGENiHBG050949.
InParanoidiQ6XQG8.
KOiK04743.
OMAiDKGAGHC.
OrthoDBiEOG091G0V3Y.
PhylomeDBiQ6XQG8.
TreeFamiTF342871.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR016469. Carbohydrate_sulfotransferase.
IPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
PIRSFiPIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
SUPFAMiSSF52540. SSF52540. 3 hits.

Sequencei

Sequence statusi: Complete.

Q6XQG8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKGRRRRRRE YCKFTLLLAL YTLLLLLVPS VLDSGSEQDK GGRDCPGLQR
60 70 80 90 100
SLGVWSLEAA AAGEREQGAE VRFQAEGNPD RSPRPQGNLS AIRESVTQEK
110 120 130 140 150
QHIYVHATWR TGSSFLGELF NQHPDVFYLY EPMWHLWQAL YPGNAESLQG
160 170 180 190 200
ALRDMLRSLF RCDFSVLRLY AQPGDPAERA PDSANLTTAM LFRWRTNKVI
210 220 230 240 250
CSPPLCPAAP RARADVGLVE DKACESTCPP VPLRALEAEC RKYPVVVIKD
260 270 280 290 300
VRLLDLGVLV PLLRDPGLNL KVVQLFRDPR AVHNSRLKSR HGLLRESIQV
310 320 330 340 350
LRTRQRGDRF QRVLLAHGVG ARPGGQSRAL PSAPRADFFL TSALEVICEA
360 370 380 390 400
WLRDLLFTRG APTWLRRRYL RLRYEDLVWQ PQVQLRRLLR FSGLRTLAAL
410 420 430 440 450
DAFAFNMTRG SAYGADRPFH LSARDAREAV HAWRERLSQE QVRQVEAACD
460 470 480
PAMRLLAYPR SGDERDVKTV RKGETPLETN ANWAT
Length:485
Mass (Da):55,091
Last modified:July 5, 2004 - v1
Checksum:iC9A9C8F961478693
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY216524 mRNA. Translation: AAP51034.1.
BC111079 mRNA. Translation: AAI11080.1.
RefSeqiNP_997483.1. NM_207600.2.
UniGeneiRn.153551.

Genome annotation databases

EnsembliENSRNOT00000005630; ENSRNOP00000005630; ENSRNOG00000004258.
GeneIDi302302.
KEGGirno:302302.
UCSCiRGD:1303028. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY216524 mRNA. Translation: AAP51034.1.
BC111079 mRNA. Translation: AAI11080.1.
RefSeqiNP_997483.1. NM_207600.2.
UniGeneiRn.153551.

3D structure databases

ProteinModelPortaliQ6XQG8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000005630.

Proteomic databases

PaxDbiQ6XQG8.
PRIDEiQ6XQG8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000005630; ENSRNOP00000005630; ENSRNOG00000004258.
GeneIDi302302.
KEGGirno:302302.
UCSCiRGD:1303028. rat.

Organism-specific databases

CTDi56548.
RGDi1303028. Chst7.

Phylogenomic databases

eggNOGiENOG410IIA6. Eukaryota.
ENOG4111DKG. LUCA.
GeneTreeiENSGT00530000062902.
HOGENOMiHOG000261614.
HOVERGENiHBG050949.
InParanoidiQ6XQG8.
KOiK04743.
OMAiDKGAGHC.
OrthoDBiEOG091G0V3Y.
PhylomeDBiQ6XQG8.
TreeFamiTF342871.

Enzyme and pathway databases

ReactomeiR-RNO-2022870. Chondroitin sulfate biosynthesis.

Miscellaneous databases

PROiQ6XQG8.

Gene expression databases

BgeeiENSRNOG00000004258.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR016469. Carbohydrate_sulfotransferase.
IPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
PIRSFiPIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
SUPFAMiSSF52540. SSF52540. 3 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiCHST7_RAT
AccessioniPrimary (citable) accession number: Q6XQG8
Secondary accession number(s): Q2TA65
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: July 5, 2004
Last modified: September 7, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.