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Q6XQG8 (CHST7_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Carbohydrate sulfotransferase 7

EC=2.8.2.-
EC=2.8.2.17
Alternative name(s):
Chondroitin 6-sulfotransferase 2
Short name=C6ST-2
Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 5
Short name=GST-5
N-acetylglucosamine 6-O-sulfotransferase 4
Short name=GlcNAc6ST-4
Short name=Gn6st-4
Gene names
Name:Chst7
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length485 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues. Preferentially acts on mannose-linked GlcNAc. Also able to catalyze the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Also acts on core 2 mucin-type oligosaccharide and N-acetyllactosamine oligomer with a lower efficiency. Has weak or no activity toward keratan sulfate and oligosaccharides containing the Galbeta1-4GlcNAc. Catalyzes 6-O-sulfation of beta-benzyl GlcNAc but not alpha- or beta-benzyl GalNAc By similarity.

Catalytic activity

3'-phosphoadenylyl sulfate + chondroitin = adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein By similarity.

Sequence similarities

Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 485485Carbohydrate sulfotransferase 7
PRO_0000085200

Regions

Topological domain1 – 1212Cytoplasmic Potential
Transmembrane13 – 3321Helical; Signal-anchor for type II membrane protein; Potential
Topological domain34 – 485452Lumenal Potential
Nucleotide binding109 – 1157PAPS By similarity
Nucleotide binding277 – 2859PAPS By similarity

Amino acid modifications

Glycosylation881N-linked (GlcNAc...) Potential
Glycosylation1851N-linked (GlcNAc...) Potential
Glycosylation4061N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q6XQG8 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: C9A9C8F961478693

FASTA48555,091
        10         20         30         40         50         60 
MKGRRRRRRE YCKFTLLLAL YTLLLLLVPS VLDSGSEQDK GGRDCPGLQR SLGVWSLEAA 

        70         80         90        100        110        120 
AAGEREQGAE VRFQAEGNPD RSPRPQGNLS AIRESVTQEK QHIYVHATWR TGSSFLGELF 

       130        140        150        160        170        180 
NQHPDVFYLY EPMWHLWQAL YPGNAESLQG ALRDMLRSLF RCDFSVLRLY AQPGDPAERA 

       190        200        210        220        230        240 
PDSANLTTAM LFRWRTNKVI CSPPLCPAAP RARADVGLVE DKACESTCPP VPLRALEAEC 

       250        260        270        280        290        300 
RKYPVVVIKD VRLLDLGVLV PLLRDPGLNL KVVQLFRDPR AVHNSRLKSR HGLLRESIQV 

       310        320        330        340        350        360 
LRTRQRGDRF QRVLLAHGVG ARPGGQSRAL PSAPRADFFL TSALEVICEA WLRDLLFTRG 

       370        380        390        400        410        420 
APTWLRRRYL RLRYEDLVWQ PQVQLRRLLR FSGLRTLAAL DAFAFNMTRG SAYGADRPFH 

       430        440        450        460        470        480 
LSARDAREAV HAWRERLSQE QVRQVEAACD PAMRLLAYPR SGDERDVKTV RKGETPLETN 


ANWAT 

« Hide

References

« Hide 'large scale' references
[1]Seko A., Yamashita K.
Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Wistar.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Thymus.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY216524 mRNA. Translation: AAP51034.1.
BC111079 mRNA. Translation: AAI11080.1.
RefSeqNP_997483.1. NM_207600.2.
UniGeneRn.153551.

3D structure databases

ProteinModelPortalQ6XQG8.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ6XQG8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000005630; ENSRNOP00000005630; ENSRNOG00000004258.
GeneID302302.
KEGGrno:302302.
UCSCRGD:1303028. rat.

Organism-specific databases

CTD56548.
RGD1303028. Chst7.

Phylogenomic databases

eggNOGNOG71363.
GeneTreeENSGT00530000062902.
HOGENOMHOG000261614.
HOVERGENHBG050949.
InParanoidQ6XQG8.
KOK04743.
OMADKGAGHC.
OrthoDBEOG7RZ5S0.
PhylomeDBQ6XQG8.
TreeFamTF342871.

Gene expression databases

GenevestigatorQ6XQG8.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR016469. Carbohydrate_sulfotransferase.
IPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
PIRSFPIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
SUPFAMSSF52540. SSF52540. 3 hits.
ProtoNetSearch...

Other

NextBio649413.
PROQ6XQG8.

Entry information

Entry nameCHST7_RAT
AccessionPrimary (citable) accession number: Q6XQG8
Secondary accession number(s): Q2TA65
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families