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Reviewed, UniProtKB/Swiss-Prot Q6XPS3 (TPTE2_HUMAN)

Last modified January 19, 2010. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase TPTE2
    EC=3.1.3.67
Alternative name(s):
    TPTE and PTEN homologous inositol lipid phosphatase
    Lipid phosphatase TPIP
Gene names
Name: TPTE2
Synonyms: TPIP
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length522 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

General annotation (Comments)

Catalytic activity

Phosphatidylinositol 3,4,5-trisphosphate + H2O = phosphatidylinositol 4,5-bisphosphate + phosphate.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.1.

Isoform 4: Cytoplasm Ref.1.

Tissue specificity

Isoform 3 is expressed in testis, brain and stomach while isoform 4 seems to be testis-specific. Ref.1

Sequence similarities

Contains 1 C2 tensin-type domain.

Contains 1 phosphatase tensin-type domain.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q6XPS3-1)

Also known as: TPIP gamma;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q6XPS3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     132-171: Missing.
Isoform 3 (identifier: Q6XPS3-3)

Also known as: TPIP alpha;

The sequence of this isoform differs from the canonical sequence as follows:
     40-77: SMLERLSKFEVEDAENVASYDSKIKKIVHSIVSSFAFG → R
     132-171: Missing.
Isoform 4 (identifier: Q6XPS3-4)

Also known as: TPIP beta;

The sequence of this isoform differs from the canonical sequence as follows:
     40-170: Missing.
     435-522: ILHDIETDKI...FAVEILFGEK → REEGSTLRRANWKGEPSRRPVLD

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 522522Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase TPTE2
PRO_0000224187

Regions

Transmembrane66 – 8621 Potential
Transmembrane111 – 13121 Potential
Transmembrane146 – 16621 Potential
Domain210 – 386177Phosphatase tensin-type
Domain393 – 522130C2 tensin-type

Sites

Active site3201Phosphocysteine intermediate Probable

Natural variations

Alternative sequence40 – 170131Missing in isoform 4.
VSP_017322
Alternative sequence40 – 7738SMLER…SFAFG → R in isoform 3.
VSP_017323
Alternative sequence132 – 17140Missing in isoform 2 and isoform 3.
VSP_017324
Alternative sequence435 – 52288ILHDI…LFGEK → REEGSTLRRANWKGEPSRRP VLD in isoform 4.
VSP_017325
Natural variant3671V → I: dbSNP rs2497218.
VAR_047501
Natural variant4441I → V: dbSNP rs2497218. Ref.2
VAR_057349

Experimental info

Mutagenesis3201C → S: Loss of activity. Ref.1
Sequence conflict5211E → K in CAD13144. Ref.1
Sequence conflict5211E → K in AAP45146. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (TPIP gamma) [UniParc].

Last modified November 25, 2008. Version 2.
Checksum: 4CC42349355EDA9F

FASTA52261,112
        10         20         30         40         50         60 
MNESPQTNEF KGTTEEAPAK ESPHTSEFKG AALVSPISKS MLERLSKFEV EDAENVASYD 

        70         80         90        100        110        120 
SKIKKIVHSI VSSFAFGIFG VFLVLLDVTL LLADLIFTDS KLYIPLEYRS ISLAIGLFFL 

       130        140        150        160        170        180 
MDVLLRVFVE GRQQYFSDLF NILDTAIIVI PLLVDVIYIF FDIKLLRNIP RWTHLVRLLR 

       190        200        210        220        230        240 
LIILIRIFHL LHQKRQLEKL MRRLVSENKR RYTRDGFDLD LTYVTERIIA MSFPSSGRQS 

       250        260        270        280        290        300 
FYRNPIEEVV RFLDKKHRNH YRVYNLCSER AYDPKHFHNR VSRIMIDDHN VPTLHEMVVF 

       310        320        330        340        350        360 
TKEVNEWMAQ DLENIVAIHC KGGKGRTGTM VCALLIASEI FLTAEESLYY FGERRTNKTH 

       370        380        390        400        410        420 
SNKFQGVETP SQNRYVGYFA QVKHLYNWNL PPRRILFIKR FIIYSIRGDV CDLKVQVVME 

       430        440        450        460        470        480 
KKVVFSSTSL GNCSILHDIE TDKILINVYD GPPLYDDVKV QFFSSNLPKY YDNCPFFFWF 

       490        500        510        520 
NTSFIQNNRL CLPRNELDNP HKQKAWKIYP PEFAVEILFG EK 

« Hide

Isoform 2.

Checksum: 7AC9220C5EAA8E7F
Show »

FASTA48256,320
Isoform 3 (TPIP alpha).

Checksum: 9808843A147E838D
Show »

FASTA44552,231
Isoform 4 (TPIP beta).

Checksum: E4E36B37921D7C7F
Show »

FASTA32638,263

References

« Hide 'large scale' references
[1]"TPIP: a novel phosphoinositide 3-phosphatase."
Walker S.M., Downes C.P., Leslie N.R.
Biochem. J. 360:277-283(2001) [PubMed: 11716755] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 3 AND 4), FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, MUTAGENESIS OF CYS-320.
Tissue: Testis.
[2]"The TPTE gene family: cellular expression, subcellular localization and alternative splicing."
Tapparel C., Reymond A., Girardet C., Guillou L., Lyle R., Lamon C., Hutter P., Antonarakis S.E.
Gene 323:189-199(2003) [PubMed: 14659893] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT VAL-444.
[3]"The DNA sequence and analysis of human chromosome 13."
Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T.
Nature 428:522-528(2004) [PubMed: 15057823] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ421032 mRNA. Translation: CAD13144.1.
AJ421033 mRNA. Translation: CAD13145.1.
AY219890 mRNA. Translation: AAP45146.1.
AL590076 Genomic DNA. Translation: CAH73538.1.
AL590076 Genomic DNA. Translation: CAH73539.1.
CH471075 Genomic DNA. Translation: EAX08219.1.
IPIIPI00418438.
IPI00480172.
IPI00719421.
IPI00935789.
RefSeqNP_001135440.1.
NP_570141.3.
NP_954863.2.
UniGeneHs.377488

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGQ6XPS3.

PTM databases

PhosphoSiteQ6XPS3.

Genome annotation databases

EnsemblENST00000400230; ENSP00000383089; ENSG00000132958; Homo sapiens. [Genome view]
GeneID93492.
KEGGhsa:93492.
UCSCuc001umd.1. human.

Organism-specific databases

CTD93492.
GeneCardsGC13M018895.
H-InvDBHIX0020751.
HIX0022787.
HIX0037315.
HIX0037502.
HIX0080243.
HGNCHGNC:17299. TPTE2.
MIM606791. gene.
PharmGKBPA134917664.
GenAtlasSearch...

Phylogenomic databases

HOGENOMHBG745257.
HOVERGENQ6XPS3.
InParanoidQ6XPS3.

Enzyme and pathway databases

BRENDA3.1.3.67. 247.

Gene expression databases

BgeeQ6XPS3.
CleanExHS_TPTE2.
GenevestigatorQ6XPS3.

Family and domain databases

InterProIPR008973. C2_Ca/lipid-bd_dom_CaLB.
IPR000340. Dual-sp_phosphatase_cat-dom.
IPR005821. Ion_trans.
IPR014019. Phosphatase_tensin-typ.
IPR014020. Tensin_phosphatase_C2-dom.
IPR016130. Tyr_Pase_AS.
[Graphical view]
PfamPF00782. DSPc. 1 hit.
PF00520. Ion_trans. 1 hit.
PF10409. PTEN_C2. 1 hit.
[Graphical view]
PROSITEPS51182. C2_TENSIN. 1 hit.
PS51181. PPASE_TENSIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio78104.
SOURCESearch...

Entry information

Entry nameTPTE2_HUMAN
AccessionPrimary (citable) accession number: Q6XPS3
Secondary accession number(s): B1AQ16 expand/collapse secondary AC list , Q5VUH2, Q8WWL4, Q8WWL5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: November 25, 2008
Last modified: January 19, 2010
This is version 59 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 13

Human chromosome 13: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents