Reviewed, UniProtKB/Swiss-Prot Q6XPS3 (TPTE2_HUMAN)

Names and origin

Protein names

Recommended name:
    Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase TPTE2
    EC=3.1.3.67
Alternative name(s):
    TPTE and PTEN homologous inositol lipid phosphatase
    Lipid phosphatase TPIP

Gene names

Name:	TPTE2
Synonyms:	TPIP

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	522 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Phosphatidylinositol 3,4,5-trisphosphate + H2O = phosphatidylinositol 4,5-bisphosphate + phosphate.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.1. Isoform 4: Cytoplasm Ref.1.
Tissue specificity	Isoform 3 is expressed in testis, brain and stomach while isoform 4 seems to be testis-specific. Ref.1
Sequence similarities	Contains 1 C2 tensin-type domain. Contains 1 phosphatase tensin-type domain.

Ontologies

Keywords
Cellular component	Cytoplasm Endoplasmic reticulum Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ion transport Inferred from electronic annotation. Source: InterPro protein amino acid dephosphorylation Inferred from electronic annotation. Source: InterPro transmembrane transport Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ion channel activity Inferred from electronic annotation. Source: InterPro phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity Inferred from electronic annotation. Source: EC protein tyrosine phosphatase activity Inferred from electronic annotation. Source: InterPro protein tyrosine/serine/threonine phosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6XPS3-1)

Also known as: TPIP gamma;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6XPS3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     132-171: Missing.

Isoform 3 (identifier: Q6XPS3-3)

Also known as: TPIP alpha;

The sequence of this isoform differs from the canonical sequence as follows:
     40-77: SMLERLSKFEVEDAENVASYDSKIKKIVHSIVSSFAFG → R
     132-171: Missing.

Isoform 4 (identifier: Q6XPS3-4)

Also known as: TPIP beta;

The sequence of this isoform differs from the canonical sequence as follows:
     40-170: Missing.
     435-522: ILHDIETDKI...FAVEILFGEK → REEGSTLRRANWKGEPSRRPVLD

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 522	522	Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase TPTE2		PRO_0000224187
Regions
	Transmembrane	66 – 86	21	Potential
	Transmembrane	111 – 131	21	Potential
	Transmembrane	146 – 166	21	Potential
	Domain	210 – 386	177	Phosphatase tensin-type
	Domain	393 – 522	130	C2 tensin-type
Sites
	Active site	320	1	Phosphocysteine intermediate Probable
Natural variations
	Alternative sequence	40 – 170	131	Missing in isoform 4.		VSP_017322
	Alternative sequence	40 – 77	38	SMLER…SFAFG → R in isoform 3.		VSP_017323
	Alternative sequence	132 – 171	40	Missing in isoform 2 and isoform 3.		VSP_017324
	Alternative sequence	435 – 522	88	ILHDI…LFGEK → REEGSTLRRANWKGEPSRRP VLD in isoform 4.		VSP_017325
	Natural variant	367	1	V → I: dbSNP rs2497218.		VAR_047501
	Natural variant	444	1	I → V: dbSNP rs2497218. Ref.2		VAR_057349
Experimental info
	Mutagenesis	320	1	C → S: Loss of activity. Ref.1
	Sequence conflict	521	1	E → K in CAD13144. Ref.1
	Sequence conflict	521	1	E → K in AAP45146. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (TPIP gamma) [UniParc]. Last modified November 25, 2008. Version 2. Checksum: 4CC42349355EDA9F	FASTA	522	61,112
	10 20 30 40 50 60 MNESPQTNEF KGTTEEAPAK ESPHTSEFKG AALVSPISKS MLERLSKFEV EDAENVASYD 70 80 90 100 110 120 SKIKKIVHSI VSSFAFGIFG VFLVLLDVTL LLADLIFTDS KLYIPLEYRS ISLAIGLFFL 130 140 150 160 170 180 MDVLLRVFVE GRQQYFSDLF NILDTAIIVI PLLVDVIYIF FDIKLLRNIP RWTHLVRLLR 190 200 210 220 230 240 LIILIRIFHL LHQKRQLEKL MRRLVSENKR RYTRDGFDLD LTYVTERIIA MSFPSSGRQS 250 260 270 280 290 300 FYRNPIEEVV RFLDKKHRNH YRVYNLCSER AYDPKHFHNR VSRIMIDDHN VPTLHEMVVF 310 320 330 340 350 360 TKEVNEWMAQ DLENIVAIHC KGGKGRTGTM VCALLIASEI FLTAEESLYY FGERRTNKTH 370 380 390 400 410 420 SNKFQGVETP SQNRYVGYFA QVKHLYNWNL PPRRILFIKR FIIYSIRGDV CDLKVQVVME 430 440 450 460 470 480 KKVVFSSTSL GNCSILHDIE TDKILINVYD GPPLYDDVKV QFFSSNLPKY YDNCPFFFWF 490 500 510 520 NTSFIQNNRL CLPRNELDNP HKQKAWKIYP PEFAVEILFG EK
	Isoform 2. Checksum: 7AC9220C5EAA8E7F	FASTA	482	56,320
	10 20 30 40 50 60 MNESPQTNEF KGTTEEAPAK ESPHTSEFKG AALVSPISKS MLERLSKFEV EDAENVASYD 70 80 90 100 110 120 SKIKKIVHSI VSSFAFGIFG VFLVLLDVTL LLADLIFTDS KLYIPLEYRS ISLAIGLFFL 130 140 150 160 170 180 MDVLLRVFVE GWTHLVRLLR LIILIRIFHL LHQKRQLEKL MRRLVSENKR RYTRDGFDLD 190 200 210 220 230 240 LTYVTERIIA MSFPSSGRQS FYRNPIEEVV RFLDKKHRNH YRVYNLCSER AYDPKHFHNR 250 260 270 280 290 300 VSRIMIDDHN VPTLHEMVVF TKEVNEWMAQ DLENIVAIHC KGGKGRTGTM VCALLIASEI 310 320 330 340 350 360 FLTAEESLYY FGERRTNKTH SNKFQGVETP SQNRYVGYFA QVKHLYNWNL PPRRILFIKR 370 380 390 400 410 420 FIIYSIRGDV CDLKVQVVME KKVVFSSTSL GNCSILHDIE TDKILINVYD GPPLYDDVKV 430 440 450 460 470 480 QFFSSNLPKY YDNCPFFFWF NTSFIQNNRL CLPRNELDNP HKQKAWKIYP PEFAVEILFG EK
	Isoform 3 (TPIP alpha). Checksum: 9808843A147E838D	FASTA	445	52,231
	10 20 30 40 50 60 MNESPQTNEF KGTTEEAPAK ESPHTSEFKG AALVSPISKR IFGVFLVLLD VTLLLADLIF 70 80 90 100 110 120 TDSKLYIPLE YRSISLAIGL FFLMDVLLRV FVEGWTHLVR LLRLIILIRI FHLLHQKRQL 130 140 150 160 170 180 EKLMRRLVSE NKRRYTRDGF DLDLTYVTER IIAMSFPSSG RQSFYRNPIE EVVRFLDKKH 190 200 210 220 230 240 RNHYRVYNLC SERAYDPKHF HNRVSRIMID DHNVPTLHEM VVFTKEVNEW MAQDLENIVA 250 260 270 280 290 300 IHCKGGKGRT GTMVCALLIA SEIFLTAEES LYYFGERRTN KTHSNKFQGV ETPSQNRYVG 310 320 330 340 350 360 YFAQVKHLYN WNLPPRRILF IKRFIIYSIR GDVCDLKVQV VMEKKVVFSS TSLGNCSILH 370 380 390 400 410 420 DIETDKILIN VYDGPPLYDD VKVQFFSSNL PKYYDNCPFF FWFNTSFIQN NRLCLPRNEL 430 440 DNPHKQKAWK IYPPEFAVEI LFGEK
	Isoform 4 (TPIP beta). Checksum: E4E36B37921D7C7F	FASTA	326	38,263
	10 20 30 40 50 60 MNESPQTNEF KGTTEEAPAK ESPHTSEFKG AALVSPISKR WTHLVRLLRL IILIRIFHLL 70 80 90 100 110 120 HQKRQLEKLM RRLVSENKRR YTRDGFDLDL TYVTERIIAM SFPSSGRQSF YRNPIEEVVR 130 140 150 160 170 180 FLDKKHRNHY RVYNLCSERA YDPKHFHNRV SRIMIDDHNV PTLHEMVVFT KEVNEWMAQD 190 200 210 220 230 240 LENIVAIHCK GGKGRTGTMV CALLIASEIF LTAEESLYYF GERRTNKTHS NKFQGVETPS 250 260 270 280 290 300 QNRYVGYFAQ VKHLYNWNLP PRRILFIKRF IIYSIRGDVC DLKVQVVMEK KVVFSSTSLG 310 320 NCSREEGSTL RRANWKGEPS RRPVLD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"TPIP: a novel phosphoinositide 3-phosphatase." Walker S.M., Downes C.P., Leslie N.R. Biochem. J. 360:277-283(2001) [PubMed: 11716755] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 3 AND 4), FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, MUTAGENESIS OF CYS-320. Tissue: Testis.
[2]	"The TPTE gene family: cellular expression, subcellular localization and alternative splicing." Tapparel C., Reymond A., Girardet C., Guillou L., Lyle R., Lamon C., Hutter P., Antonarakis S.E. Gene 323:189-199(2003) [PubMed: 14659893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT VAL-444.
[3]	"The DNA sequence and analysis of human chromosome 13." Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T. Nature 428:522-528(2004) [PubMed: 15057823] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ421032 mRNA. Translation: CAD13144.1. AJ421033 mRNA. Translation: CAD13145.1. AY219890 mRNA. Translation: AAP45146.1. AL590076 Genomic DNA. Translation: CAH73538.1. AL590076 Genomic DNA. Translation: CAH73539.1. CH471075 Genomic DNA. Translation: EAX08219.1.
IPI	IPI00418438. IPI00480172. IPI00719421. IPI00935789.
RefSeq	NP_001135440.1. NP_570141.3. NP_954863.2.
UniGene	Hs.377488
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q6XPS3.
PTM databases
PhosphoSite	Q6XPS3.
Genome annotation databases
Ensembl	ENST00000400230; ENSP00000383089; ENSG00000132958; Homo sapiens. [Genome view]
GeneID	93492.
KEGG	hsa:93492.
UCSC	uc001umd.1. human.
Organism-specific databases
CTD	93492.
GeneCards	GC13M018895.
H-InvDB	HIX0020751. HIX0022787. HIX0037315. HIX0037502. HIX0080243.
HGNC	HGNC:17299. TPTE2.
MIM	606791. gene.
PharmGKB	PA134917664.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	HBG745257.
HOVERGEN	Q6XPS3.
InParanoid	Q6XPS3.
Enzyme and pathway databases
BRENDA	3.1.3.67. 247.
Gene expression databases
Bgee	Q6XPS3.
CleanEx	HS_TPTE2.
Genevestigator	Q6XPS3.
Family and domain databases
InterPro	IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR000340. Dual-sp_phosphatase_cat-dom. IPR005821. Ion_trans. IPR014019. Phosphatase_tensin-typ. IPR014020. Tensin_phosphatase_C2-dom. IPR016130. Tyr_Pase_AS. [Graphical view]
Pfam	PF00782. DSPc. 1 hit. PF00520. Ion_trans. 1 hit. PF10409. PTEN_C2. 1 hit. [Graphical view]
PROSITE	PS51182. C2_TENSIN. 1 hit. PS51181. PPASE_TENSIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	78104.
SOURCE	Search...

Entry information

Entry name

TPTE2_HUMAN

Accession

Primary (citable) accession number: Q6XPS3
Secondary accession number(s): B1AQ16 , Q5VUH2, Q8WWL4, Q8WWL5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2006
Last sequence update:	November 25, 2008
Last modified:	January 19, 2010
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.