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Protein

Zinc finger protein GLIS3

Gene

Glis3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as both a repressor and activator of transcription. Binds to the consensus sequence 5'-GACCACCCAC-3'.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri345 – 370C2H2-type 1PROSITE-ProRule annotationAdd BLAST26
Zinc fingeri379 – 406C2H2-type 2; atypicalPROSITE-ProRule annotationAdd BLAST28
Zinc fingeri412 – 436C2H2-type 3PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri442 – 466C2H2-type 4PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri472 – 496C2H2-type 5PROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

  • DNA binding Source: MGI
  • DNA binding transcription factor activity Source: MGI
  • metal ion binding Source: UniProtKB-KW
  • RNA polymerase II proximal promoter sequence-specific DNA binding Source: NTNU_SB
  • RNA polymerase II regulatory region sequence-specific DNA binding Source: MGI
  • transcriptional activator activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: NTNU_SB
  • transcriptional repressor activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: NTNU_SB

GO - Biological processi

  • negative regulation of transcription by RNA polymerase II Source: UniProtKB
  • positive regulation of transcription by RNA polymerase II Source: UniProtKB
  • transcription by RNA polymerase II Source: UniProtKB

Keywordsi

Molecular functionActivator, DNA-binding, Repressor
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein GLIS3
Alternative name(s):
GLI-similar 3
Gene namesi
Name:Glis3Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:2444289 Glis3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000472121 – 780Zinc finger protein GLIS3Add BLAST780

Proteomic databases

PaxDbiQ6XP49
PRIDEiQ6XP49

PTM databases

iPTMnetiQ6XP49
PhosphoSitePlusiQ6XP49

Expressioni

Tissue specificityi

In the embryo, expressed at high levels in the kidney and testis. In the adult, expressed at high levels in the kidney and uterus and at lower levels in the brain, lung, skeletal muscle and pancreas.1 Publication

Developmental stagei

In the embryo, expression is detected between days 6.5 and 14.5, particularly during neurulation. At embryonic days 11.5 to 12.5, expression is high in the interdigital regions destined to undergo apoptosis.1 Publication

Gene expression databases

BgeeiENSMUSG00000052942
CleanExiMM_GLIS3
ExpressionAtlasiQ6XP49 baseline and differential

Interactioni

Protein-protein interaction databases

BioGridi230466, 1 interactor
CORUMiQ6XP49
STRINGi10090.ENSMUSP00000124635

Structurei

3D structure databases

ProteinModelPortaliQ6XP49
SMRiQ6XP49
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi490 – 506Bipartite nuclear localization signalSequence analysisAdd BLAST17

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi290 – 320Pro-richAdd BLAST31
Compositional biasi545 – 660Pro-richAdd BLAST116

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri345 – 370C2H2-type 1PROSITE-ProRule annotationAdd BLAST26
Zinc fingeri379 – 406C2H2-type 2; atypicalPROSITE-ProRule annotationAdd BLAST28
Zinc fingeri412 – 436C2H2-type 3PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri442 – 466C2H2-type 4PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri472 – 496C2H2-type 5PROSITE-ProRule annotationAdd BLAST25

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00900000140802
HOGENOMiHOG000231136
HOVERGENiHBG107950
InParanoidiQ6XP49

Family and domain databases

InterProiView protein in InterPro
IPR030405 GLIS3
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PANTHERiPTHR19818:SF123 PTHR19818:SF123, 1 hit
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 5 hits
SUPFAMiSSF57667 SSF57667, 3 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 4 hits
PS50157 ZINC_FINGER_C2H2_2, 5 hits

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6XP49-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMVQRLGPIS PPASQVSTAC KQISPSLPRA VNAANLNRPP SDTRSVILQE
60 70 80 90 100
SLVSTTLSLT ESQSALSVKQ EWSQSYRAFP SLSSSHSSQN GTDLGDLLSL
110 120 130 140 150
PPGTPVSGNS VSNSLPPYLF GMENSHSPYP SPRHSATRAH STRSKKRALS
160 170 180 190 200
LSPLSDGIGI DFNTIIRTSP TSLVAYINGP RASPANLSPQ SEVYGHFLGV
210 220 230 240 250
RGSCIPQSCA VASGQKGILV ASGGHTLPGY GEDGTLEYER MQQLEHGGLQ
260 270 280 290 300
PGPVNNMVLQ PGLPGQDGQT ANMLKTERLE EFPASALDLP SALPLPLPPP
310 320 330 340 350
QGPPPPYHAH PHLHHPELLP HTQSLSLAQT GLEEDGEMED SGGKHCCRWI
360 370 380 390 400
DCSALYDQQE ELVRHIEKVH IDQRKGEDFT CFWTGCPRRY KPFNARYKLL
410 420 430 440 450
IHMRVHSGEK PNKCTFEGCK KAFSRLENLK IHLRSHTGEK PYLCQHPGCQ
460 470 480 490 500
KAFSNSSDRA KHQRTHLDTK PYACQIPGCT KRYTDPSSLR KHVKAHSSRE
510 520 530 540 550
QQARKKLRSS TELHPDLLTD CLAVQPLQPA TSPGDAADHT VGHSPGPGPG
560 570 580 590 600
PGPGAELYSA PIFASNHSTR SGTAAGAGPP PHPVSHPSPG HNVQGSPHNP
610 620 630 640 650
SSQLPPLTAV DAGAERFAPP TPSPHHISPG RVPAPPSLLQ RAQAPHSQQP
660 670 680 690 700
PGSLLKPYQP ETNSSFQPNG IHVHGFYGQL QTFCPPHYPD SQRTVPPSGS
710 720 730 740 750
CSMVPSFEDC LVPTSMGQAG FDVFHRAFST HSGITVYDLP SASSSLFGES
760 770 780
LRSGPEDPTF LQLSAVDRCP SQLSSVYTEG
Length:780
Mass (Da):83,882
Last modified:December 21, 2004 - v2
Checksum:i9A15F8FBC1775787
GO
Isoform 2 (identifier: Q6XP49-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MNGRSCGMNL...NNLVVTSSPM
     508-516: RSSTELHPD → TNICASSHW
     517-780: Missing.

Note: No experimental confirmation available.
Show »
Length:671
Mass (Da):72,374
Checksum:i8F513939670626C0
GO

Sequence cautioni

The sequence AAP59883 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti274L → P in BAC35623 (PubMed:16141072).Curated1
Sequence conflicti295L → I in BAC35623 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0122811M → MNGRSCGMNLHRTSRTPQGP GLLGGQHIPPIRAHAGTPCS SSCASTPSPSIGSLANSLHL KMSSGAGMAPQSNMAASPIH LPALSPRRQLLANGKPQFQV TPAGVMAAPHTIKPKQQEFG DPFSPNPEKGALGFGPQCKS IGKGSCNNLVVTSSPM in isoform 2. 1 Publication1
Alternative sequenceiVSP_012282508 – 516RSSTELHPD → TNICASSHW in isoform 2. 1 Publication9
Alternative sequenceiVSP_012283517 – 780Missing in isoform 2. 1 PublicationAdd BLAST264

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY220846 mRNA Translation: AAP59883.1 Different initiation.
AK054020 mRNA Translation: BAC35623.1
RefSeqiXP_006527048.1, XM_006526985.3 [Q6XP49-1]
UniGeneiMm.223508
Mm.459186

Genome annotation databases

EnsembliENSMUST00000112612; ENSMUSP00000108231; ENSMUSG00000052942 [Q6XP49-2]
GeneIDi226075
UCSCiuc008hch.2 mouse [Q6XP49-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGLIS3_MOUSE
AccessioniPrimary (citable) accession number: Q6XP49
Secondary accession number(s): Q8BI60
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: December 21, 2004
Last modified: March 28, 2018
This is version 102 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health