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Protein

Zinc finger protein GLIS3

Gene

Glis3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as both a repressor and activator of transcription. Binds to the consensus sequence 5'-GACCACCCAC-3'.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri345 – 370C2H2-type 1PROSITE-ProRule annotationAdd BLAST26
Zinc fingeri379 – 406C2H2-type 2; atypicalPROSITE-ProRule annotationAdd BLAST28
Zinc fingeri412 – 436C2H2-type 3PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri442 – 466C2H2-type 4PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri472 – 496C2H2-type 5PROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

GO - Biological processi

  • negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • transcription from RNA polymerase II promoter Source: UniProtKB

Keywordsi

Molecular functionActivator, DNA-binding, Repressor
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein GLIS3
Alternative name(s):
GLI-similar 3
Gene namesi
Name:Glis3Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:2444289. Glis3.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

  • nucleus Source: UniProtKB

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000472121 – 780Zinc finger protein GLIS3Add BLAST780

Proteomic databases

PaxDbiQ6XP49.
PRIDEiQ6XP49.

PTM databases

iPTMnetiQ6XP49.
PhosphoSitePlusiQ6XP49.

Expressioni

Tissue specificityi

In the embryo, expressed at high levels in the kidney and testis. In the adult, expressed at high levels in the kidney and uterus and at lower levels in the brain, lung, skeletal muscle and pancreas.1 Publication

Developmental stagei

In the embryo, expression is detected between days 6.5 and 14.5, particularly during neurulation. At embryonic days 11.5 to 12.5, expression is high in the interdigital regions destined to undergo apoptosis.1 Publication

Gene expression databases

BgeeiENSMUSG00000052942.
CleanExiMM_GLIS3.
ExpressionAtlasiQ6XP49. baseline and differential.

Interactioni

Protein-protein interaction databases

BioGridi230466. 1 interactor.
STRINGi10090.ENSMUSP00000124635.

Structurei

3D structure databases

ProteinModelPortaliQ6XP49.
SMRiQ6XP49.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi490 – 506Bipartite nuclear localization signalSequence analysisAdd BLAST17

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi290 – 320Pro-richAdd BLAST31
Compositional biasi545 – 660Pro-richAdd BLAST116

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri345 – 370C2H2-type 1PROSITE-ProRule annotationAdd BLAST26
Zinc fingeri379 – 406C2H2-type 2; atypicalPROSITE-ProRule annotationAdd BLAST28
Zinc fingeri412 – 436C2H2-type 3PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri442 – 466C2H2-type 4PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri472 – 496C2H2-type 5PROSITE-ProRule annotationAdd BLAST25

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00760000118771.
HOGENOMiHOG000231136.
HOVERGENiHBG107950.
InParanoidiQ6XP49.

Family and domain databases

Gene3Di3.30.160.60. 5 hits.
InterProiView protein in InterPro
IPR030405. GLIS3.
IPR013087. Znf_C2H2_type.
PANTHERiPTHR19818:SF113. PTHR19818:SF113. 1 hit.
SMARTiView protein in SMART
SM00355. ZnF_C2H2. 5 hits.
SUPFAMiSSF57667. SSF57667. 3 hits.
PROSITEiView protein in PROSITE
PS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 5 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6XP49-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMVQRLGPIS PPASQVSTAC KQISPSLPRA VNAANLNRPP SDTRSVILQE
60 70 80 90 100
SLVSTTLSLT ESQSALSVKQ EWSQSYRAFP SLSSSHSSQN GTDLGDLLSL
110 120 130 140 150
PPGTPVSGNS VSNSLPPYLF GMENSHSPYP SPRHSATRAH STRSKKRALS
160 170 180 190 200
LSPLSDGIGI DFNTIIRTSP TSLVAYINGP RASPANLSPQ SEVYGHFLGV
210 220 230 240 250
RGSCIPQSCA VASGQKGILV ASGGHTLPGY GEDGTLEYER MQQLEHGGLQ
260 270 280 290 300
PGPVNNMVLQ PGLPGQDGQT ANMLKTERLE EFPASALDLP SALPLPLPPP
310 320 330 340 350
QGPPPPYHAH PHLHHPELLP HTQSLSLAQT GLEEDGEMED SGGKHCCRWI
360 370 380 390 400
DCSALYDQQE ELVRHIEKVH IDQRKGEDFT CFWTGCPRRY KPFNARYKLL
410 420 430 440 450
IHMRVHSGEK PNKCTFEGCK KAFSRLENLK IHLRSHTGEK PYLCQHPGCQ
460 470 480 490 500
KAFSNSSDRA KHQRTHLDTK PYACQIPGCT KRYTDPSSLR KHVKAHSSRE
510 520 530 540 550
QQARKKLRSS TELHPDLLTD CLAVQPLQPA TSPGDAADHT VGHSPGPGPG
560 570 580 590 600
PGPGAELYSA PIFASNHSTR SGTAAGAGPP PHPVSHPSPG HNVQGSPHNP
610 620 630 640 650
SSQLPPLTAV DAGAERFAPP TPSPHHISPG RVPAPPSLLQ RAQAPHSQQP
660 670 680 690 700
PGSLLKPYQP ETNSSFQPNG IHVHGFYGQL QTFCPPHYPD SQRTVPPSGS
710 720 730 740 750
CSMVPSFEDC LVPTSMGQAG FDVFHRAFST HSGITVYDLP SASSSLFGES
760 770 780
LRSGPEDPTF LQLSAVDRCP SQLSSVYTEG
Length:780
Mass (Da):83,882
Last modified:December 21, 2004 - v2
Checksum:i9A15F8FBC1775787
GO
Isoform 2 (identifier: Q6XP49-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MNGRSCGMNL...NNLVVTSSPM
     508-516: RSSTELHPD → TNICASSHW
     517-780: Missing.

Note: No experimental confirmation available.
Show »
Length:671
Mass (Da):72,374
Checksum:i8F513939670626C0
GO

Sequence cautioni

The sequence AAP59883 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti274L → P in BAC35623 (PubMed:16141072).Curated1
Sequence conflicti295L → I in BAC35623 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0122811M → MNGRSCGMNLHRTSRTPQGP GLLGGQHIPPIRAHAGTPCS SSCASTPSPSIGSLANSLHL KMSSGAGMAPQSNMAASPIH LPALSPRRQLLANGKPQFQV TPAGVMAAPHTIKPKQQEFG DPFSPNPEKGALGFGPQCKS IGKGSCNNLVVTSSPM in isoform 2. 1 Publication1
Alternative sequenceiVSP_012282508 – 516RSSTELHPD → TNICASSHW in isoform 2. 1 Publication9
Alternative sequenceiVSP_012283517 – 780Missing in isoform 2. 1 PublicationAdd BLAST264

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY220846 mRNA. Translation: AAP59883.1. Different initiation.
AK054020 mRNA. Translation: BAC35623.1.
RefSeqiXP_006527048.1. XM_006526985.3. [Q6XP49-1]
UniGeneiMm.223508.
Mm.459186.

Genome annotation databases

EnsembliENSMUST00000112612; ENSMUSP00000108231; ENSMUSG00000052942. [Q6XP49-2]
GeneIDi226075.
UCSCiuc008hch.2. mouse. [Q6XP49-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY220846 mRNA. Translation: AAP59883.1. Different initiation.
AK054020 mRNA. Translation: BAC35623.1.
RefSeqiXP_006527048.1. XM_006526985.3. [Q6XP49-1]
UniGeneiMm.223508.
Mm.459186.

3D structure databases

ProteinModelPortaliQ6XP49.
SMRiQ6XP49.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230466. 1 interactor.
STRINGi10090.ENSMUSP00000124635.

PTM databases

iPTMnetiQ6XP49.
PhosphoSitePlusiQ6XP49.

Proteomic databases

PaxDbiQ6XP49.
PRIDEiQ6XP49.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000112612; ENSMUSP00000108231; ENSMUSG00000052942. [Q6XP49-2]
GeneIDi226075.
UCSCiuc008hch.2. mouse. [Q6XP49-1]

Organism-specific databases

CTDi169792.
MGIiMGI:2444289. Glis3.

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00760000118771.
HOGENOMiHOG000231136.
HOVERGENiHBG107950.
InParanoidiQ6XP49.

Miscellaneous databases

ChiTaRSiGlis3. mouse.
PROiPR:Q6XP49.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000052942.
CleanExiMM_GLIS3.
ExpressionAtlasiQ6XP49. baseline and differential.

Family and domain databases

Gene3Di3.30.160.60. 5 hits.
InterProiView protein in InterPro
IPR030405. GLIS3.
IPR013087. Znf_C2H2_type.
PANTHERiPTHR19818:SF113. PTHR19818:SF113. 1 hit.
SMARTiView protein in SMART
SM00355. ZnF_C2H2. 5 hits.
SUPFAMiSSF57667. SSF57667. 3 hits.
PROSITEiView protein in PROSITE
PS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 5 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiGLIS3_MOUSE
AccessioniPrimary (citable) accession number: Q6XP49
Secondary accession number(s): Q8BI60
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: December 21, 2004
Last modified: April 12, 2017
This is version 97 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.