Q6XMH7 (AMPM2_ENCIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine aminopeptidase 2
Short name=MAP-2
Short name=MetAP 2
EC=3.4.11.18

Alternative name(s):
Peptidase M 2

Gene names

Name:

MAP2

Organism

Encephalitozoon intestinalis (Microsporidian parasite)

Taxonomic identifier

58839 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Microsporidia › Unikaryonidae › Encephalitozoon

Protein attributes

Sequence length	358 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Removes the amino-terminal methionine from nascent proteins.
Catalytic activity	Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Cofactor	Binds 2 cobalt ions per subunit. The true nature of the physiological cofactor is under debate. The enzyme is also active with zinc, manganese or divalent iron ions By similarity.
Sequence similarities	Belongs to the peptidase M24A family.

Ontologies

Keywords
Ligand	Cobalt Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Gene Ontology (GO)
Biological process	cellular process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 358	358	Methionine aminopeptidase 2		PRO_0000148987
Sites
	Metal binding	130	1	Cobalt 1 By similarity
	Metal binding	141	1	Cobalt 1 By similarity
	Metal binding	141	1	Cobalt 2 By similarity
	Metal binding	210	1	Cobalt 2 By similarity
	Metal binding	243	1	Cobalt 2 By similarity
	Metal binding	339	1	Cobalt 1 By similarity
	Metal binding	339	1	Cobalt 2 By similarity
	Binding site	109	1	Substrate By similarity
	Binding site	218	1	Substrate By similarity
Experimental info
	Sequence conflict	24	1	K → R in AAR04555. Ref.2
	Sequence conflict	27	1	K → R in AAR04555. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q6XMH7 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 8900021059AA4D6E	FASTA	358	40,001
	10 20 30 40 50 60 MKFILIDQAP KLPIEFLPKG DCYKKGKLFG PKGEEIENTT DCDLLQDARR AAEAHRRARY 70 80 90 100 110 120 KVQSIIRPGI TLLEIVRSIE DSTRTLLEGE RNNGIGFPAG MSMNSCAAHY TVNPGEEDIV 130 140 150 160 170 180 LKEDDVLKVD FGTHSNGRIM DSAFTVAFQE NLQPLLMAAR EGTETGIRSL GIDARVCDIG 190 200 210 220 230 240 RDINEVITSY EVEIEGKTWP IRPVSDLHGH SISQFKIHGG ISIPAVNNRD TTRIKGDTFY 250 260 270 280 290 300 AVETFATTGK GFINDRSPCS HFMLNVHKSR KLFNKDLIKV YEFVKSSFGT LPFSPRHLDH 310 320 330 340 350 YNLVEGGSLK SVNLLTMMGL FTPYPPLNDI DGSKVAQFEH TVYLSENGKE ILTRGDDY

References

[1]	"Characterization of recombinant microsporidian methionine aminopeptidase type 2." Weiss L.M., Zhou G.C., Zhang H. J. Eukaryot. Microbiol. 50:597-599(2003) [PubMed: 14736176] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Phylogenetic relationships of methionine aminopeptidase 2 among Encephalitozoon species and genotypes of microsporidia." Pandrea I., Mittleider D., Brindley P.J., Didier E.S., Robertson D.L. Mol. Biochem. Parasitol. 140:141-152(2005) [PubMed: 15760654] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 14-331.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY224693 Genomic DNA. Translation: AAP51022.1. AY339781 Genomic DNA. Translation: AAR04555.1.
3D structure databases
ProteinModelPortal	Q6XMH7.
SMR	Q6XMH7. Positions 4-358.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BRENDA	3.4.11.18. 8683.
Family and domain databases
InterPro	IPR000994. Pept_M24_structural-domain. IPR002468. Pept_M24A_MAP2. IPR018349. Pept_M24A_MAP2_BS. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:3.90.230.10. Peptidase_M24_cat_core. 2 hits. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
PANTHER	PTHR10804:SF9. Pept_M24A_MAP2. 1 hit.
Pfam	PF00557. Peptidase_M24. 1 hit. [Graphical view]
SUPFAM	SSF55920. Peptidase_M24_cat_core. 1 hit.
TIGRFAMs	TIGR00501. Met_pdase_II. 1 hit.
PROSITE	PS01202. MAP_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPM2_ENCIN

Accession

Primary (citable) accession number: Q6XMH7
Secondary accession number(s): Q6VH15

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	July 5, 2004
Last modified:	November 16, 2011
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program