Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q6XDK8

- POLG_SVM10

UniProt

Q6XDK8 - POLG_SVM10

Protein

Genome polyprotein

Gene

ORF1

Organism
Sapporo virus (isolate GII/Human/Thailand/Mc10/2000) (Hu/SaV/Mc10/2000/Thailand)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    NTPase presumably plays a role in replication. Despite having similarities with helicases, does not seem to display any helicase activity By similarity.By similarity
    Viral genome-linked protein is covalently linked to the 5'-end of the positive-strand, negative-strand genomic RNAs and subgenomic RNA. Acts as a genome-linked replication primer. May recruit ribosome to viral RNA thereby promoting viral proteins translation By similarity.By similarity
    Protease-polymerase p70 processes the polyprotein: Pro-Pol is first released by autocleavage, then all other proteins are cleaved By similarity. It is also an RNA-directed RNA polymerase which replicates genomic and antigenomic viral RNA by recognizing specific signals. Catalyzes the covalent attachment VPg with viral RNAs By similarity.By similarity
    Capsid protein self assembles to form an icosahedral capsid with a T=3 symmetry, about 38 nm in diameter, and consisting of 180 capsid proteins. The capsid encapsulate the genomic RNA and VP2 proteins. Attaches virion to target cells, inducing endocytosis of the viral particle. Acidification of the endosome induces conformational change of capsid protein thereby injecting virus genomic RNA into host cytoplasm By similarity.By similarity

    Catalytic activityi

    NTP + H2O = NDP + phosphate.
    Endopeptidase with a preference for cleavage when the P1 position is occupied by Glu-|-Xaa and the P1' position is occupied by Gly-|-Yaa.
    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei69 – 702Cleavage; by Pro-Pol
    Sitei325 – 3262Cleavage; by Pro-Pol
    Sitei666 – 6672Cleavage; by Pro-Pol
    Sitei940 – 9412Cleavage; by Pro-Pol
    Sitei1055 – 10562Cleavage; by Pro-Pol
    Active sitei1086 – 10861For protease activityBy similarity
    Active sitei1107 – 11071For protease activityBy similarity
    Active sitei1171 – 11711For protease activityBy similarity
    Sitei1722 – 17232Cleavage; by Pro-Pol

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi481 – 4888ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cysteine-type endopeptidase activity Source: InterPro
    3. RNA binding Source: InterPro
    4. RNA-directed RNA polymerase activity Source: UniProtKB-KW
    5. RNA helicase activity Source: InterPro

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW
    2. RNA-protein covalent cross-linking Source: UniProtKB-KW
    3. transcription, DNA-templated Source: InterPro
    4. viral RNA genome replication Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase

    Keywords - Biological processi

    DNA replication, Viral RNA replication

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Protein family/group databases

    MEROPSiC24.003.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Genome polyprotein
    Cleaved into the following 7 chains:
    Alternative name(s):
    p35
    Alternative name(s):
    VPg
    p14
    Protease-polymerase p70 (EC:2.7.7.48, EC:3.4.22.66)
    Short name:
    Pro-Pol
    Capsid protein
    Short name:
    CP
    Alternative name(s):
    VP1
    p60
    Gene namesi
    ORF Names:ORF1
    OrganismiSapporo virus (isolate GII/Human/Thailand/Mc10/2000) (Hu/SaV/Mc10/2000/Thailand)
    Taxonomic identifieri234601 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageCaliciviridaeSapovirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]

    Subcellular locationi

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell
    2. viral capsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid protein, Host cytoplasm, Virion

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi1069 – 10691H → A: No effect on protease activity in vitro. 1 Publication
    Mutagenesisi1075 – 10751H → A: No effect on protease activity in vitro. 1 Publication
    Mutagenesisi1086 – 10861H → A: Complete loss of protease activity in vitro. 1 Publication
    Mutagenesisi1107 – 11071E → A: Complete loss of protease activity in vitro. 1 Publication
    Mutagenesisi1120 – 11201H → A: No effect on protease activity in vitro. 1 Publication
    Mutagenesisi1136 – 11361H → A: No effect on protease activity in vitro. 1 Publication
    Mutagenesisi1143 – 11431E → A: No effect on protease activity in vitro. 1 Publication
    Mutagenesisi1147 – 11471E → A: No effect on protease activity in vitro. 1 Publication
    Mutagenesisi1171 – 11711C → A: Complete loss of protease activity in vitro. 1 Publication
    Mutagenesisi1186 – 11861H → A: No effect on protease activity in vitro. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 22782278Genome polyproteinPRO_0000342101Add
    BLAST
    Chaini1 – 6969Protein p11PRO_0000342102Add
    BLAST
    Chaini70 – 325256Protein p28PRO_0000342103Add
    BLAST
    Chaini326 – 666341NTPasePRO_0000342104Add
    BLAST
    Chaini667 – 940274Protein p32PRO_0000342105Add
    BLAST
    Chaini941 – 1055115Viral genome-linked proteinPRO_0000342106Add
    BLAST
    Chaini1056 – 1722667Protease-polymerase p70PRO_0000342107Add
    BLAST
    Chaini1723 – 2278556Capsid proteinPRO_5000090469Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei966 – 9661O-(5'-phospho-RNA)-tyrosineBy similarity

    Post-translational modificationi

    Specific enzymatic cleavages in vivo yield mature proteins. Pro-Pol is first autocatalytically cleaved, then processes the whole polyprotein.1 Publication
    VPg is uridylylated by the polymerase and is covalently attached to the 5'-end of the polyadenylated genomic and subgenomic RNAs. This uridylylated form acts as a nucleotide-peptide primer for the polymerase By similarity.By similarity

    Keywords - PTMi

    Covalent protein-RNA linkage, Phosphoprotein

    Interactioni

    Subunit structurei

    Capsid protein homodimerizes, then multimerizes.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ6XDK8.
    SMRiQ6XDK8. Positions 1209-1703.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini454 – 609156SF3 helicasePROSITE-ProRule annotationAdd
    BLAST
    Domaini1071 – 1177107Peptidase C24Add
    BLAST
    Domaini1443 – 1568126RdRp catalyticPROSITE-ProRule annotationAdd
    BLAST

    Domaini

    Protease-polymerase is composed of two domains displaying two different catalytic activity. These activities may act independently.

    Sequence similaritiesi

    Contains 1 peptidase C24 domain.Curated
    Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation
    Contains 1 SF3 helicase domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di2.60.120.20. 1 hit.
    3.40.50.300. 2 hits.
    InterProiIPR004005. Calicivirus_coat.
    IPR004004. Helic/Pol/Pept_Calicivir-typ.
    IPR000605. Helicase_SF3_ssDNA/RNA_vir.
    IPR014759. Helicase_SF3_ssRNA_vir.
    IPR027417. P-loop_NTPase.
    IPR000317. Peptidase_C24.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009003. Trypsin-like_Pept_dom.
    IPR029053. Viral_coat.
    [Graphical view]
    PfamiPF00915. Calici_coat. 1 hit.
    PF03510. Peptidase_C24. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF00910. RNA_helicase. 1 hit.
    [Graphical view]
    PRINTSiPR00916. 2CENDOPTASE.
    PR00918. CALICVIRUSNS.
    SUPFAMiSSF50494. SSF50494. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEiPS50507. RDRP_SSRNA_POS. 1 hit.
    PS51218. SF3_HELICASE_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative promoter usage. Align

    Isoform Genome polyprotein (identifier: Q6XDK8-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MASKPFYPIE FNPSVELQVL RSAHLRVGGR EQMFETINDL NDHVRGVVAK     50
    LWCKHLHRSL AAAPTFTEEG LLDSFLSKPP VDINPDTTFR ELFGINPHEQ 100
    FPLSIHDLAK LQGELVDAAR NPGHVLRRHY STDSLTALIN KITKFVPVHA 150
    TLQEMQARRA FERERAELFR ELPHADLDVS RQQKSYFYAM WRQVVKKSKE 200
    FFIPLVKCTS WRKKFTEPAE IVRQVLVHFC EGMRSQFSTN ANYINLSLIA 250
    KLRPTVLTMI LQQHKNTYRG WLATVTALVE VYSNLFQDMR DTAVSAVSAI 300
    TLVFETIKDF VVNVIDLVKS TFQSQGPTSC GWAAIIAGAL LILMKLSGCS 350
    NTTSYWHRLL KVCGGVTTIA AAARAVVWVR DIIAEADGKA RLKKYMARTA 400
    ALLELAASRD VTGTDELKRL LDCFTQLIEE GTELIQEFGT SPLAGLTRSY 450
    VSELESTANS IRSTILLDTP RKTPVAIILT GPPGIGKTRL AQHLAAGFGK 500
    VSNFSVTLDH HDSYTGNEVA IWDEFDVDTQ GKFVETMIGV VNTAPYPLNC 550
    DRVENKGKVF TSDYIICTSN YPTSVLPDNP RAGAFYRRVT TIDVSSPTIE 600
    DWKKKNPGKK PPPDLYKNDF THLRLSVRPF LGYNPEGDTL DGVRVKPVLT 650
    SVDGLSRLME TKFKEQGNEH RNLWITCPRD LVAPAASGLK AYMAANRALA 700
    QVFQEPSSQD IGETCTSRVY VSCNNPPPTY SGRVVKITAI NPWDASLANS 750
    MLSMFETTSH IPASIQREIM YRVWDPLVHL QTREPNTQML PYINRVVPVS 800
    SAFDFIRGLR HHLGLCSVKG MWRAYQGWNS SSSILEFLSK HMADVAFPHN 850
    PECTVFRAPD GDVIFYTFGS YACFVSPARV PFVGEPPKNV HSNITRNMTW 900
    AETLRLLAET ITESLVHFGP FLLMMHNVSY LATRSGREEE AKGKTKHGRG 950
    AKHARRGGVS LSDDEYDEWR DLVRDWRQDM TVGEFVELRE RYALGMDSED 1000
    VQRYRAWLEL RAMRMGAGAY QHATIIGRGG VQDTIIRTQP MRAPRAPRNQ 1050
    GYDEEAPTPI VTFTSGGDHI GYGCHMGNGV VVTVTHVASA SDQVEGQDFA 1100
    IRKTEGETTW VNTNLGHLPH YQIGDGAPVY YSARLHPVTT LAEGTYETPN 1150
    ITVQGYHLRI INGYPTKRGD CGTPYFDSCR RLVGLHAATS TNGETKLAQR 1200
    VTKTSKVENA FAWKGLPVVR GPDCGGMPTG TRYHRSPAWP NPVEGETHAP 1250
    APFGSGDERY KFSQVEMLVN GLKPYSEPTP GIPPALLQRA ATHTRTYLET 1300
    IIGTHRSPNL SFSEACSLLE KSTSCGPFVA GQKGDYWDED KQCYTGVLAE 1350
    HLAKAWDAAN RGVAPQNAYK LALKDELRPI EKNAQGKRRL LWGCDAGATL 1400
    VATAAFKGVA TRLQAVAPMT PVSVGINMDS YQVEVLNESL KGGVLYCLDY 1450
    SKWDSTQHPA VTAASLGILE RLSEATPITT SAVELLSSPA RGHLNDIVFI 1500
    TKSGLPSGMP FTSVINSLNH MTYFAAAVLK AYEQHGAPYT GNVFQVETVH 1550
    TYGDDCLYSV CPATASIFQT VLANLTSFGL KPTAADKSET IAPTHTPVFL 1600
    KRTLTCTPRG VRGLLDITSI KRQFLWIKAN RTVDINSPPA YDRDARGIQL 1650
    ENALAYASQH GHAVFEEVAE LARHTAKAEG LVLTNVNYDQ ALATYESWFI 1700
    GGTGLVQGSP SEETTKLVFE MEGLGQPQPQ GGEKTSPQPV TPQDTIGPTA 1750
    ALLLPTQIET PNASAQRLEL AMATGAVTSN VPNCIRECFA SVTTIPWTTR 1800
    QAANTFLGAI HLGPRINPYT AHLSAMFAGW GGGFQVRVTI SGSGLFAGRA 1850
    VTAILPPGVN PASVQNPGVF PHAFIDARTT EPILINLPDI RPVDFHRVDG 1900
    DDATASVGLW VAQPLINPFQ TGPVSTCWLS FETRPGPDFD FCLLKAPEQQ 1950
    MDNGISPASL LPRRLGRSRG NRMGGRIVGL VVVAAAEQVN HHFDARSTTL 2000
    GWSTLPVEPI AGDISWYGDA GNKSIRGLVS AQGKGIIFPN IVNHWTDVAL 2050
    SSKTSNTTTI PTDTSTLGNL PGASGPLVTF ADNGDVNESS AQNAILTAAN 2100
    QNFTSFSPTF DAAGIWVWMP WATDRPGASD SNIYISPTWV NGNPSHPIHE 2150
    KCTNMIGTNF QFGGTGTNNI MLWQEQHFTS WPGAAEVYCS QLESTAEIFQ 2200
    NNIVNIPMNQ MAVFNVETAG NSFQIAILPN GYCVTNAPVG THQLLDYETS 2250
    FKFVGLFPQS TSLQGPHGNS GRAVRFLE 2278

    Note: Produced from the genomic RNA.

    Length:2,278
    Mass (Da):250,097
    Last modified:July 5, 2004 - v1
    Checksum:iDE7386F8EE49AD11
    GO
    Isoform Subgenomic capsid protein (identifier: Q6XDK8-2) [UniParc]FASTAAdd to Basket

    Also known as: VP1

    The sequence of this isoform differs from the canonical sequence as follows:
         1-1720: Missing.

    Note: Produced from the subgenomic RNA.

    Show »
    Length:558
    Mass (Da):59,560
    Checksum:i56CFD514ADE23DED
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 17201720Missing in isoform Subgenomic capsid protein. CuratedVSP_034390Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY237420 mRNA. Translation: AAQ17058.2.
    RefSeqiYP_022762.1. NC_010624.1. [Q6XDK8-1]

    Genome annotation databases

    GeneIDi2943313.

    Keywords - Coding sequence diversityi

    Alternative promoter usage

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY237420 mRNA. Translation: AAQ17058.2 .
    RefSeqi YP_022762.1. NC_010624.1. [Q6XDK8-1 ]

    3D structure databases

    ProteinModelPortali Q6XDK8.
    SMRi Q6XDK8. Positions 1209-1703.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C24.003.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 2943313.

    Family and domain databases

    Gene3Di 2.60.120.20. 1 hit.
    3.40.50.300. 2 hits.
    InterProi IPR004005. Calicivirus_coat.
    IPR004004. Helic/Pol/Pept_Calicivir-typ.
    IPR000605. Helicase_SF3_ssDNA/RNA_vir.
    IPR014759. Helicase_SF3_ssRNA_vir.
    IPR027417. P-loop_NTPase.
    IPR000317. Peptidase_C24.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009003. Trypsin-like_Pept_dom.
    IPR029053. Viral_coat.
    [Graphical view ]
    Pfami PF00915. Calici_coat. 1 hit.
    PF03510. Peptidase_C24. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF00910. RNA_helicase. 1 hit.
    [Graphical view ]
    PRINTSi PR00916. 2CENDOPTASE.
    PR00918. CALICVIRUSNS.
    SUPFAMi SSF50494. SSF50494. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEi PS50507. RDRP_SSRNA_POS. 1 hit.
    PS51218. SF3_HELICASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], PROTEOLYTIC PROCESSING OF POLYPROTEIN, MUTAGENESIS OF CYS-1171.
    2. "Highly conserved configuration of catalytic amino acid residues among calicivirus-encoded proteases."
      Oka T., Yamamoto M., Yokoyama M., Ogawa S., Hansman G.S., Katayama K., Miyashita K., Takagi H., Tohya Y., Sato H., Takeda N.
      J. Virol. 81:6798-6806(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF HIS-1069; HIS-1075; HIS-1086; GLU-1107; HIS-1120; HIS-1136; GLU-1143; GLU-1147 AND HIS-1186.
    3. "Cleavage activity of the sapovirus 3C-like protease in Escherichia coli."
      Oka T., Katayama K., Ogawa S., Hansman G.S., Kageyama T., Miyamura T., Takeda N.
      Arch. Virol. 150:2539-2548(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1056-1060.

    Entry informationi

    Entry nameiPOLG_SVM10
    AccessioniPrimary (citable) accession number: Q6XDK8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 2008
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Two differents RNAs lead the expression of the capsid protein. One arises from the cleavage of the polyprotein translated from the genomic RNA and the other from the translation of a subgenomic RNA derived from the (-)RNA template. Capsid protein expressed from the subgenomic mRNA is produced in much larger amounts than the cleaved one By similarity.By similarity

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3