Reviewed, UniProtKB/Swiss-Prot Q6X2E0 (DMAW1_CLAPU)

Names and origin

Protein names

Recommended name:
    Tryptophan dimethylallyltransferase 1
    EC=2.5.1.34
Alternative name(s):
    L-tryptophan dimethylallyl transferase 1
      Short name=DMATS 1
    All-trans-hexaprenyl-diphosphate synthase 1

Gene names

Name:	dmaW1
Synonyms:	cpd1, dmaw-1

Organism

Claviceps purpurea (Ergot fungus) (Sphacelia purpurea)

Taxonomic identifier

5111 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Hypocreomycetidae › Hypocreales › Clavicipitaceae › Claviceps

Protein attributes

Sequence length	448 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the first step of ergot alkaloid biosynthesis. Ergot alkaloids, which are produced by endophyte fungi, can enhance plant host fitness, but also cause livestock toxicosis to host plants.
Catalytic activity	Dimethylallyl diphosphate + L-tryptophan = diphosphate + 4-(3-methylbut-2-enyl)-L-tryptophan.
Pathway	Alkaloid biosynthesis; ergot alkaloid biosynthesis.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the tryptophan dimethylallyltransferase family.

Ontologies

Keywords
Biological process	Alkaloid metabolism
Coding sequence diversity	Alternative splicing
Molecular function	Transferase
Gene Ontology (GO)
Biological process	alkaloid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	tryptophan dimethylallyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6X2E0-1)

Also known as: Short;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6X2E0-2)

Also known as: Long;

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MCETEVDSLSCPKVNAA

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 448	448	Tryptophan dimethylallyltransferase 1		PRO_0000181364
Natural variations
	Alternative sequence	1	1	M → MCETEVDSLSCPKVNAA in isoform 2.		VSP_011244
	Natural variant	116	1	T → P in strain: T5.
	Natural variant	124	1	T → A in strain: T5.
	Natural variant	211	1	L → F in strain: T5.
	Natural variant	240	1	V → S in strain: T5.
	Natural variant	244	1	G → I in strain: T5.
	Natural variant	342	1	D → N in strain: T5.
	Natural variant	380	1	N → K in strain: T5.
	Natural variant	409 – 430	22	RRNKP…RWPVF → WPVGESTELYQHGLRKLNNF LV in strain: T5.

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Short) [UniParc]. Last modified August 16, 2004. Version 2. Checksum: 625709FAAF269242	FASTA	448	51,673
	10 20 30 40 50 60 MSTAKDPGNG VYEILSLIFD FPSNEQRLWW HSTAPMFAAM LDNAGYNIHD QYRHLGIFKK 70 80 90 100 110 120 HIIPFLGVYP TKDKERWLSI LTRCGLPLEL SLNCTDSVVR YTYEPINEVT GTEKDTFNTL 130 140 150 160 170 180 AIMTSVQKLA QIQAGIDLEW FSYFKDELTL DESESATLQS NELVKEQIKT QNKLALDLKE 190 200 210 220 230 240 SQFALKVYFY PHLKSIATGK STHDLIFDSV LKLSQKHDSI QPAFQVLCDY VSRRNHSAEV 250 260 270 280 290 300 DQHGALHARL LSCDLIDPAK SRVKIYLLEK TVSLSVMEDL WTLGGQRVDA STMDGLDMLR 310 320 330 340 350 360 ELWSLLKVPT GHLEYPKGYL ELGEIPNEQL PSMANYTLHH NDPMPEPQVY FTVFGMNDAE 370 380 390 400 410 420 ISNALTIFFQ RHGFDDMAKN YRVFLQDSYP YHDFESLNYL HAYISFSYRR NKPYLSVYLH 430 440 TFETGRWPVF ADSPISFDAY RRCELSTK
	Isoform 2 (Long). Checksum: 221F487CCF444D69	FASTA	464	53,321
	10 20 30 40 50 60 MCETEVDSLS CPKVNAASTA KDPGNGVYEI LSLIFDFPSN EQRLWWHSTA PMFAAMLDNA 70 80 90 100 110 120 GYNIHDQYRH LGIFKKHIIP FLGVYPTKDK ERWLSILTRC GLPLELSLNC TDSVVRYTYE 130 140 150 160 170 180 PINEVTGTEK DTFNTLAIMT SVQKLAQIQA GIDLEWFSYF KDELTLDESE SATLQSNELV 190 200 210 220 230 240 KEQIKTQNKL ALDLKESQFA LKVYFYPHLK SIATGKSTHD LIFDSVLKLS QKHDSIQPAF 250 260 270 280 290 300 QVLCDYVSRR NHSAEVDQHG ALHARLLSCD LIDPAKSRVK IYLLEKTVSL SVMEDLWTLG 310 320 330 340 350 360 GQRVDASTMD GLDMLRELWS LLKVPTGHLE YPKGYLELGE IPNEQLPSMA NYTLHHNDPM 370 380 390 400 410 420 PEPQVYFTVF GMNDAEISNA LTIFFQRHGF DDMAKNYRVF LQDSYPYHDF ESLNYLHAYI 430 440 450 460 SFSYRRNKPY LSVYLHTFET GRWPVFADSP ISFDAYRRCE LSTK

References

[1]	"Evidence for an ergot alkaloid gene cluster in Claviceps purpurea." Tudzynski P., Hoelter K., Correia T.H., Arntz C., Grammel N., Keller U. Mol. Gen. Genet. 261:133-141(1999) [PubMed: 10071219] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1). Strain: P1.
[2]	"The determinant step in ergot alkaloid biosynthesis by an endophyte of perennial ryegrass." Wang J., Machado C., Panaccione D.G., Tsai H.-F., Schardl C.L. Fungal Genet. Biol. 41:189-198(2004) [PubMed: 14732265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2). Strain: ATCC 20102.
[3]	"Molecular analysis of dimethyl-allyl-tryptophan-synthase-genes." Correia T.H., Tudzynski P. Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-441 (ISOFORM 1). Strain: T5.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ011963 Genomic DNA. Translation: CAB39314.1. AY259840 Genomic DNA. Translation: AAP81209.1. AY259840 Genomic DNA. Translation: AAP81210.1. AJ312754 Genomic DNA. Translation: CAC37397.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.5.1.34. 1795.
Family and domain databases
InterPro	IPR017795. Aromatic_prenylTrfase_DMATS. IPR012148. Trp_dimethylallyltransferase. IPR017796. Trp_dimethylallylTrfase_sub. [Graphical view]
PIRSF	PIRSF000509. Trp_DMAT. 1 hit.
TIGRFAMs	TIGR03429. arom_pren_DMATS. 1 hit. TIGR03430. trp_dimet_allyl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DMAW1_CLAPU

Accession

Primary (citable) accession number: Q6X2E0
Secondary accession number(s): O94204, Q9C140

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	August 16, 2004
Last modified:	June 16, 2009
This is version 24 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)