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Protein

Protein VP3

Gene
N/A
Organism
Rotavirus A (strain RVA/Human/United States/Wa/1974/G1P1A[8]) (RV-A)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Multifunctional enzyme involved in mRNA capping. Catalyzes the formation of the 5' cap structure on the viral plus-strand transcripts. Specifically binds to GTP and displays guanylyltransferase and methyltransferase activities. Has affinity for ssRNA but not for dsRNA. Capping activity is non-specific and caps RNAs that initiate with either a G or an A residue. Together with VP1 polymerase, forms a VP1-VP3 complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are capped by VP3 and extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. DLPs probably have an RNA triphosphatase activity as well, whereas open cores do not.UniRule annotation
Counteracts the host innate immune response thanks to its phosphodiesterase that degrades the 5'-triphosphorylated, 2'-5' linked adenylate oligomers produced by the host cell IFN-inducible 2',5'-oligoadenylate synthetase (OAS). The host RNaseL is therefore not activated.UniRule annotation

Catalytic activityi

GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.UniRule annotation
S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.UniRule annotation
5'-triphosphoadenylyl-(2'->5')-adenylyl-(2'->5')-adenosine + 2 H2O = 2 AMP + ATP + 2 H+.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei718For 2'-5'-phosphodiesterase activityUniRule annotation1
Active sitei720For 2'-5'-phosphodiesterase activityUniRule annotation1
Active sitei797For 2'-5'-phosphodiesterase activityUniRule annotation1
Active sitei799For 2'-5'-phosphodiesterase activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Methyltransferase, Multifunctional enzyme, Nucleotidyltransferase, RNA-binding, Transferase
Biological processHost-virus interaction, Inhibition of host innate immune response by virus, mRNA capping, mRNA processing, Viral immunoevasion
LigandGTP-binding, Nucleotide-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Protein VP3UniRule annotation
Including the following 3 domains:
2',5'-phosphodiesteraseUniRule annotation (EC:3.1.4.-UniRule annotation)
mRNA guanylyltransferaseUniRule annotation (EC:2.7.7.50UniRule annotation)
mRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.56UniRule annotation)
OrganismiRotavirus A (strain RVA/Human/United States/Wa/1974/G1P1A[8]) (RV-A)
Taxonomic identifieri10962 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000006581 Componenti: Genome

Subcellular locationi

  • Virion UniRule annotation
  • Note: Attached inside the inner capsid as a minor component. There are about 11 to 12 copies per virion.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003680871 – 835Protein VP3Add BLAST835

Proteomic databases

PRIDEiQ6WVH5

Interactioni

Subunit structurei

Interacts with VP1. Interacts with VP2.UniRule annotation

Structurei

3D structure databases

SMRiQ6WVH5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni171 – 245N7-methyltransferase activityUniRule annotationAdd BLAST75
Regioni246 – 4282'-O-methyltransferase activityUniRule annotationAdd BLAST183
Regioni429 – 555N7-methyltransferase activityUniRule annotationAdd BLAST127
Regioni556 – 692GTase/RTPase activityUniRule annotationAdd BLAST137
Regioni693 – 8352'-5'-phosphodiesterase activityUniRule annotationAdd BLAST143

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi270 – 273Poly-Tyr4

Domaini

Contains a bipartite N7-methytransferase domain, a 2'-O-methytransferase domain and a GTase/RTPase domain. The C-terminus contains a phosphodiesterase domain that degrades the 5'-triphosphorylated, 2'-5' linked adenylate oligomers produced by the host cell in response to IFN stimulation.UniRule annotation

Sequence similaritiesi

Belongs to the rotavirus VP3 family.UniRule annotation

Phylogenomic databases

OrthoDBiVOG0900001Q

Family and domain databases

HAMAPiMF_04124 Rota_VP3, 1 hit
MF_04128 Rota_VP3_A, 1 hit
InterProiView protein in InterPro
IPR011181 VP3_Rotav
PfamiView protein in Pfam
PF06929 Rotavirus_VP3, 1 hit
PIRSFiPIRSF004015 LigT_rotavirus, 1 hit
PROSITEiView protein in PROSITE
PS51589 SAM_MT56_VP3, 1 hit

Sequencei

Sequence statusi: Complete.

Q6WVH5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVLALRHSV AQVYADTQTY LHDDSKDEYE NAFLISNLTT HNILYLNYSL
60 70 80 90 100
KTLKILNKSG IAAVEVQSPD ELFALIRCNF TYDYEDNIVY LHDYSYYTNN
110 120 130 140 150
EIRTDQHWIT KTDIIDYLLP GWKLTYVGYN GKNTRGHYNF SFICQNAATD
160 170 180 190 200
DDIIIEYIYS NELDFQNFLL RKIKERMTTS LPIARLSNRV FRDKLFPSIV
210 220 230 240 250
NIHKKVINVG PRNESMFTFL NFPTIKQFSN GAYIVKHTIK LKQEKWLGKR
260 270 280 290 300
VSQFDIGQYK NMLNVVTTIY YYYNLYYSKP IIYMLGSAPS YWIYDIKQYS
310 320 330 340 350
DFTFETWDPL DTPYSTTHHK ELFFDKDVNK LKDNSVLYID IRTDRGNMDW
360 370 380 390 400
KEWRKIVEQQ TVSNLNIAYK YLSTGKAKVC CVKLTAMDLE LPITAKLLHH
410 420 430 440 450
PTTEVRSEFY AILDVWDIIT IKRFIPKGVF YAFINNVTTE NVFIQPPFKL
460 470 480 490 500
KTSPTDYIVA LYALSNDLNS RQDVINLINK QKQSLITVRI NNTFKDEPKV
510 520 530 540 550
NFKNIYDWTF LPTDFELKDS IITSYDGCLG IFGLSISLSS KPTGNNHLFI
560 570 580 590 600
INGTDKYDKL DQYANHMGVS RRSHQIRFSE SATSYSGYIF RDLSNNNFNL
610 620 630 640 650
IGTNVENSVS GHVYNALIYY RYNYAFDLKR WIYLHSIGKV AVEGGRYYEH
660 670 680 690 700
APIELIYACR SAKEFAILQD DLTVLRYANE IEGYINKVYS ITYADDPNYF
710 720 730 740 750
IGIKFNSIPY EYDVKIPHLT LGVLFISDNM IHDVITVLKK MKTELFKMEI
760 770 780 790 800
STSYTYMLSD NTYVANASGV LSTYFKLYNM FYRNHITFGQ SRMFIPHITL
810 820 830
SFSNKQTVRI ESTKLRINSI YLRKIKGETV FDMSE
Length:835
Mass (Da):97,727
Last modified:July 5, 2004 - v1
Checksum:i3B3C6D920E4844CA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY267335 Genomic RNA Translation: AAQ02692.1
AJ292379 Genomic RNA Translation: CAB98137.1

Similar proteinsi

Entry informationi

Entry nameiVP3_ROTHW
AccessioniPrimary (citable) accession number: Q6WVH5
Secondary accession number(s): Q9ICZ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 5, 2004
Last modified: May 23, 2018
This is version 36 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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