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Protein

Polypeptide N-acetylgalactosaminyltransferase 1

Gene

GalNAc-T1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. It can both act as a peptide transferase that transfers GalNAc onto unmodified peptide substrates, and as a glycopeptide transferase that requires the prior addition of a GalNAc on a peptide before adding additional GalNAc moieties. Prefers the monoglycosylated Muc5AC-3 as substrate.

Catalytic activityi

UDP-N-acetyl-alpha-D-galactosamine + polypeptide = UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide.1 Publication

Cofactori

Mn2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei189SubstrateBy similarity1
Binding sitei221SubstrateBy similarity1
Metal bindingi244ManganeseBy similarity1
Metal bindingi246ManganeseBy similarity1
Binding sitei358SubstrateBy similarity1
Metal bindingi386ManganeseBy similarity1
Binding sitei389SubstrateBy similarity1
Binding sitei392SubstrateBy similarity1
Binding sitei394SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • oligosaccharide biosynthetic process Source: UniProtKB
  • protein glycosylation Source: UniProtKB-UniPathway

Keywordsi

Molecular functionGlycosyltransferase, Transferase
LigandLectin, Manganese, Metal-binding

Enzyme and pathway databases

BRENDAi2.4.1.41 1994
ReactomeiR-DME-913709 O-linked glycosylation of mucins
UniPathwayiUPA00378

Protein family/group databases

CAZyiCBM13 Carbohydrate-Binding Module Family 13
GT27 Glycosyltransferase Family 27

Names & Taxonomyi

Protein namesi
Recommended name:
Polypeptide N-acetylgalactosaminyltransferase 1 (EC:2.4.1.41)
Short name:
pp-GaNTase 1
Alternative name(s):
Protein-UDP acetylgalactosaminyltransferase 1
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1
Gene namesi
Name:GalNAc-T1
Synonyms:pgant1
ORF Names:CG8182
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0034025 GalNAc-T1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 7CytoplasmicSequence analysis7
Transmembranei8 – 28Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini29 – 601LumenalSequence analysisAdd BLAST573

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000591551 – 601Polypeptide N-acetylgalactosaminyltransferase 1Add BLAST601

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi127N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi138 ↔ 381PROSITE-ProRule annotation
Disulfide bondi372 ↔ 450PROSITE-ProRule annotation
Disulfide bondi485 ↔ 504PROSITE-ProRule annotation
Disulfide bondi528 ↔ 546PROSITE-ProRule annotation
Disulfide bondi571 ↔ 588PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ6WV20
PRIDEiQ6WV20

Expressioni

Tissue specificityi

Expressed in developing oocytes and egg chambers. No expression observed during embryonic stages 9-11. During embryonic stages 12-13, specific expression is observed in the developing tracheal branches and brain. During embryonic stages 14-17, expression is restricted to the dorsal longitudinal trachea. In third instar larvae imaginal wing disk, expressed in clusters of cells in the presumptive pleura and notum. In eye-antennal imaginal disk, shows a very distinct band of expression at the morphogenetic furrow and weaker expression in the presumptive eye posterior to the furrow, no expression is detected in the presumptive antennal or head region anterior to the furrow. No expression observed in leg or haltere imaginal disks.2 Publications

Developmental stagei

Expressed both maternally and zygotically. Expressed throughout embryonic, larval, pupal and adult stages, with increasing levels during larval development.2 Publications

Gene expression databases

BgeeiFBgn0034025
ExpressionAtlasiQ6WV20 differential
GenevisibleiQ6WV20 DM

Interactioni

Protein-protein interaction databases

BioGridi62452, 2 interactors
IntActiQ6WV20, 3 interactors
STRINGi7227.FBpp0086464

Structurei

3D structure databases

ProteinModelPortaliQ6WV20
SMRiQ6WV20
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini472 – 600Ricin B-type lectinPROSITE-ProRule annotationAdd BLAST129

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni148 – 260Catalytic subdomain AAdd BLAST113
Regioni327 – 389Catalytic subdomain BAdd BLAST63

Domaini

There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.By similarity
The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.By similarity

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3736 Eukaryota
ENOG410XPMK LUCA
GeneTreeiENSGT00760000118828
InParanoidiQ6WV20
KOiK00710
OMAiDHRNPLC
OrthoDBiEOG091G085O
PhylomeDBiQ6WV20

Family and domain databases

CDDicd00161 RICIN, 1 hit
Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR001173 Glyco_trans_2-like
IPR029044 Nucleotide-diphossugar_trans
IPR035992 Ricin_B-like_lectins
IPR000772 Ricin_B_lectin
PfamiView protein in Pfam
PF00535 Glycos_transf_2, 1 hit
PF00652 Ricin_B_lectin, 1 hit
SMARTiView protein in SMART
SM00458 RICIN, 1 hit
SUPFAMiSSF50370 SSF50370, 1 hit
SSF53448 SSF53448, 1 hit
PROSITEiView protein in PROSITE
PS50231 RICIN_B_LECTIN, 1 hit

Sequencei

Sequence statusi: Complete.

Q6WV20-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPRFRSFYG KLIIFILVAL CFILYSKVQQ NGSPEEPPVA PLVRAAALRG
60 70 80 90 100
HGRERFEAYS DSENEIARPA TQSPYEQIIQ LDLQKQKVGL GEQGVAVHLS
110 120 130 140 150
GAAKERGDEI YKKIALNEEL SEQLTYNRSV GDHRNPLCAK QRFDSDSLPT
160 170 180 190 200
ASVVIIFFNE PYSVLLRTVH STLSTCNEKA LKEIILVDDG SDNVELGAKL
210 220 230 240 250
DYYVRTRIPS GKVTILRLKN RLGLIRARLA GARIATGDVL IFLDAHCEGN
260 270 280 290 300
IGWCEPLLQR IKESRTSVLV PIIDVIDAND FQYSTNGYKS FQVGGFQWNG
310 320 330 340 350
HFDWINLPER EKQRQRRECK QEREICPAYS PTMAGGLFAI DRRYFWEVGS
360 370 380 390 400
YDEQMDGWGG ENLEMSFRIW QCGGTIETIP CSRVGHIFRD FHPYKFPNDR
410 420 430 440 450
DTHGINTARM ALVWMDEYIN IFFLNRPDLK FHADIGDVTH RVMLRKKLRC
460 470 480 490 500
KSFEWYLKNI YPEKFVPTKD VQGWGKVHAV NSNICLDDLL QNNEKPYNAG
510 520 530 540 550
LYPCGKVLQK SQLFSFTNTN VLRNELSCAT VQHSESPPYR VVMVPCMEND
560 570 580 590 600
EFNEQWRYEH QHIIHSNTGM CLDHQGLKSL DDAQVAPCDP HSESQRWTIE

H
Length:601
Mass (Da):68,961
Last modified:August 16, 2004 - v2
Checksum:i81BAF81E04C35BC2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti146D → E in AAQ56699 (PubMed:12829714).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY268063 mRNA Translation: AAQ56699.1
AF218236 mRNA Translation: AAG13184.1
AE013599 Genomic DNA Translation: AAM70974.1
AY113411 mRNA Translation: AAM29416.1
RefSeqiNP_611043.1, NM_137199.3
NP_725472.1, NM_166099.2
UniGeneiDm.3404

Genome annotation databases

EnsemblMetazoaiFBtr0087331; FBpp0086464; FBgn0034025
FBtr0087332; FBpp0086465; FBgn0034025
GeneIDi36717
KEGGidme:Dmel_CG8182

Similar proteinsi

Entry informationi

Entry nameiGALT1_DROME
AccessioniPrimary (citable) accession number: Q6WV20
Secondary accession number(s): Q0E964, Q9V7C8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: March 28, 2018
This is version 122 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health