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Q6WRY5

- Q6WRY5_ENTFL

UniProt

Q6WRY5 - Q6WRY5_ENTFL

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Protein

D-alanine--D-alanine ligase

Gene
vanG, ddl
Organism
Enterococcus faecalis (Streptococcus faecalis)
Status
Unreviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Cell wall formation By similarity.UniRule annotationSAAS annotations

Catalytic activityi

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.UniRule annotationSAAS annotations

Cofactori

Binds 2 magnesium or manganese ions per subunit By similarity.UniRule annotationSAAS annotations

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei140 – 1401ADPImported
Binding sitei190 – 1901ADPImported
Binding sitei226 – 2261ADPImported
Binding sitei261 – 2611ADPImported
Metal bindingi299 – 2991Magnesium or manganese 1 By similarityUniRule annotation
Binding sitei301 – 3011ADPImported
Metal bindingi312 – 3121Magnesium or manganese 1 By similarityUniRule annotation
Metal bindingi312 – 3121Magnesium or manganese 2 By similarityUniRule annotation
Metal bindingi314 – 3141Magnesium or manganese 2 By similarityUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi173 – 22856ATP By similarityUniRule annotationAdd
BLAST
Nucleotide bindingi181 – 1833ADPImported
Nucleotide bindingi219 – 2224ADPImported
Nucleotide bindingi311 – 3122ADPImported

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. D-alanine-D-alanine ligase activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. manganese ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. peptidoglycan biosynthetic process Source: UniProtKB-HAMAP
  2. regulation of cell shape Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationSAAS annotationsImported

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradationUniRule annotationSAAS annotations, Peptidoglycan synthesisUniRule annotationSAAS annotations

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotations, MagnesiumUniRule annotationSAAS annotations, ManganeseUniRule annotationSAAS annotations, Metal-bindingUniRule annotationSAAS annotations, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15480.
UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanine--D-alanine ligaseUniRule annotation (EC:6.3.2.4UniRule annotation)
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene namesi
Name:vanGImported
Synonyms:ddlUniRule annotation
OrganismiEnterococcus faecalis (Streptococcus faecalis)Imported
Taxonomic identifieri1351 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus

Subcellular locationi

Cytoplasm By similarity UniRule annotationSAAS annotations

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotations

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4FU0X-ray2.35A/B1-349[»]

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini144 – 345202ATP-grasp By similarityUniRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 ATP-grasp domain.UniRule annotation
Contains ATP-grasp domain.SAAS annotations

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPiMF_00047. Dala_Dala_lig.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERiPTHR23132. PTHR23132. 1 hit.
PfamiPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6WRY5-1 [UniParc]FASTAAdd to Basket

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MQNKKIAVIF GGNSTEYEVS LQSASAVFEN INTNKFDIIP IGITRSGEWY    50
HYTGEKEKIL NNTWFEDSKN LCPVVVSQNR SVKGFLEIAS DKYRIIKVDL 100
VFPVLHGKNG EDGTLQGIFE LAGIPVVGCD TLSSALCMDK DRAHKLVSLA 150
GISVPKSVTF KRFNEEAAMK EIEANLTYPL FIKPVRAGSS FGITKVIEKQ 200
ELDAAIELAF EHDTEVIVEE TINGFEVGCA VLGIDELIVG RVDEIELSSG 250
FFDYTEKYTL KSSKIYMPAR IDAEAEKRIQ EAAVTIYKAL GCSGFSRVDM 300
FYTPSGEIVF NEVNTIPGFT SHSRYPNMMK GIGLSFSQML DKLIGLYVE 349
Length:349
Mass (Da):38,730
Last modified:July 5, 2004 - v1
Checksum:i458EABDDF90493FD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY271782 Genomic DNA. Translation: AAQ16273.1.
DQ212986 Genomic DNA. Translation: ABA71731.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY271782 Genomic DNA. Translation: AAQ16273.1 .
DQ212986 Genomic DNA. Translation: ABA71731.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4FU0 X-ray 2.35 A/B 1-349 [» ]
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00219 .
BioCyci MetaCyc:MONOMER-15480.

Family and domain databases

Gene3Di 3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPi MF_00047. Dala_Dala_lig.
InterProi IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view ]
PANTHERi PTHR23132. PTHR23132. 1 hit.
Pfami PF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view ]
SUPFAMi SSF52440. SSF52440. 1 hit.
TIGRFAMsi TIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The vanG glycopeptide resistance operon from Enterococcus faecalis revisited."
    Depardieu F., Bonora M.G., Reynolds P.E., Courvalin P.
    Mol. Microbiol. 50:931-948(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: BM4518Imported.
  2. "VanG-type vancomycin-resistant Enterococcus faecalis strains isolated in Canada."
    Boyd D.A., Du T., Hizon R., Kaplen B., Murphy T., Tyler S., Brown S., Jamieson F., Weiss K., Mulvey M.R.
    Antimicrob. Agents Chemother. 50:2217-2221(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: G1-01247Imported.
  3. "Structural and functional characterization of VanG D-Ala:D-Ser ligase associated with vancomycin resistance in Enterococcus faecalis."
    Meziane-Cherif D., Saul F.A., Haouz A., Courvalin P.
    J. Biol. Chem. 287:37583-37592(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS) IN COMPLEX WITH ADP.

Entry informationi

Entry nameiQ6WRY5_ENTFL
AccessioniPrimary (citable) accession number: Q6WRY5
Entry historyi
Integrated into UniProtKB/TrEMBL: July 5, 2004
Last sequence update: July 5, 2004
Last modified: September 3, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported

External Data

Dasty 3

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