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Protein

Sn1-specific diacylglycerol lipase alpha

Gene

Dagla

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of diacylglycerol (DAG) to 2-arachidonoyl-glycerol (2-AG), the most abundant endocannabinoid in tissues. Required for axonal growth during development and for retrograde synaptic signaling at mature synapses (By similarity).By similarity

Cofactori

Ca2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei472 – 4721Charge relay systemPROSITE-ProRule annotation
Active sitei524 – 5241Charge relay systemPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • arachidonic acid metabolic process Source: MGI
  • diacylglycerol catabolic process Source: MGI
  • endocannabinoid signaling pathway Source: MGI
  • neuroblast proliferation Source: MGI
  • neurogenesis Source: MGI
  • neurotransmitter biosynthetic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-426048. Arachidonate production from DAG.

Protein family/group databases

ESTHERimouse-q6wqj1. Lipase_3.

Names & Taxonomyi

Protein namesi
Recommended name:
Sn1-specific diacylglycerol lipase alpha (EC:3.1.1.-)
Short name:
DGL-alpha
Alternative name(s):
Neural stem cell-derived dendrite regulator
Gene namesi
Name:Dagla
Synonyms:Kiaa0659, Nsddr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:2677061. Dagla.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2222CytoplasmicSequence analysisAdd
BLAST
Transmembranei23 – 4321HelicalSequence analysisAdd
BLAST
Topological domaini44 – 6017ExtracellularSequence analysisAdd
BLAST
Transmembranei61 – 8121HelicalSequence analysisAdd
BLAST
Topological domaini82 – 10120CytoplasmicSequence analysisAdd
BLAST
Transmembranei102 – 12221HelicalSequence analysisAdd
BLAST
Topological domaini123 – 13614ExtracellularSequence analysisAdd
BLAST
Transmembranei137 – 15721HelicalSequence analysisAdd
BLAST
Topological domaini158 – 1044887CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: UniProtKB-SubCell
  • varicosity Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL5180.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10441044Sn1-specific diacylglycerol lipase alphaPRO_0000248348Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi133 – 1331N-linked (GlcNAc...)Sequence analysis
Modified residuei728 – 7281PhosphoserineBy similarity
Modified residuei730 – 7301PhosphoserineCombined sources
Modified residuei733 – 7331PhosphoserineCombined sources
Modified residuei744 – 7441PhosphoserineCombined sources
Modified residuei784 – 7841PhosphoserineBy similarity
Modified residuei786 – 7861PhosphoserineCombined sources
Modified residuei808 – 8081PhosphoserineBy similarity
Modified residuei835 – 8351PhosphoserineBy similarity
Modified residuei849 – 8491PhosphoserineBy similarity
Modified residuei954 – 9541PhosphoserineCombined sources
Modified residuei1025 – 10251PhosphothreonineCombined sources

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ6WQJ1.
PaxDbiQ6WQJ1.
PRIDEiQ6WQJ1.

PTM databases

iPTMnetiQ6WQJ1.
PhosphoSiteiQ6WQJ1.
SwissPalmiQ6WQJ1.

Expressioni

Tissue specificityi

Highly expressed by principal cells in the hyppocampus. In embryonic brains, it is present in axonal tracts, while in adults it localizes to dendritic fields, correlating with the developmental change in requirement for 2-AG synthesis from the pre- to the postsynaptic compartment. Concentrated in heads of dendritic spines throughout the hippocampal formation. Highly compartmentalized into a wide perisynaptic annulus around the postsynaptic density of axospinous contacts but not intrasynaptically (at protein level).2 Publications

Gene expression databases

BgeeiENSMUSG00000035735.
CleanExiMM_DAGLA.
ExpressionAtlasiQ6WQJ1. baseline and differential.
GenevisibleiQ6WQJ1. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000046358.

Structurei

3D structure databases

ProteinModelPortaliQ6WQJ1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi315 – 34935Cys-richAdd
BLAST

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. Lipase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2088. Eukaryota.
ENOG410XRFI. LUCA.
GeneTreeiENSGT00530000063682.
HOGENOMiHOG000015738.
HOVERGENiHBG081435.
InParanoidiQ6WQJ1.
KOiK13806.
OMAiEPRESMQ.
OrthoDBiEOG091G04AM.
TreeFamiTF312928.

Family and domain databases

Gene3Di3.40.50.1820. 3 hits.
InterProiIPR029058. AB_hydrolase.
IPR002921. Fungal_lipase-like.
[Graphical view]
PfamiPF01764. Lipase_3. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00120. LIPASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6WQJ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGIVVFRRR WSVGSDDLVL PAIFLFLLHT TWFVILSVVL FGLVYNPHEA
60 70 80 90 100
CSLNLVDHGR GYLGILLSCM IAEMAIIWLS MRGGILYTEP RDSMQYVLYV
110 120 130 140 150
RLAILVIEFI YAIVGIVWLT QYYTSCNDLT AKNVTLGMVV CNWVVILSVC
160 170 180 190 200
ITVLCVFDPT GRTFVKLRAT KRRQRNLRTY NLRHRLEEGQ ATSWSRRLKV
210 220 230 240 250
FLCCTRTKDS QSDAYSEIAY LFAEFFRDLD IVPSDIIAGL VLLRQRQRAK
260 270 280 290 300
RNAVLDEANN DILAFLSGMP VTRNTKYLDL KNSHEMLRYK EVCYYMLFAL
310 320 330 340 350
AAYGWPMYLM RKPTCGLCQL ARSCSCCLCP ARPRFAPGVT IEEDNCCGCN
360 370 380 390 400
AIAIRRHFLD ENMTAVDIVY TSCHDAVYET PFYVAVDHDK KKVVISIRGT
410 420 430 440 450
LSPKDALTDL TGDAERLPVE GHRGTWLGHK GMVLSAEYIK KKLEQEMVLS
460 470 480 490 500
QAFGRDLGRG TKHYGLIVVG HSLGAGTAAI LSFLLRPQYP TLKCFAYSPP
510 520 530 540 550
GGLLSEDAME YSKEFVTAVV LGKDLVPRIG LSQLEGFRRQ LLDVLQRSTK
560 570 580 590 600
PKWRIIVGAT KCIPKSELPE DQVEVTTLAS TRLWTHPSDL TIALSASTPL
610 620 630 640 650
YPPGRIIHVV HNHPAEQCCC CEQEEPTYFA IWGDNKAFNE VIISPAMLHE
660 670 680 690 700
HLPYVVMEGL NKVLENYNKG KTALLSAAKV MVSPTEVDLT PELIFQQQPL
710 720 730 740 750
PTGPPLPTGL ALELPATEHR NSSVRSKSQS EMSLEGFSEG RLLSPVAAAS
760 770 780 790 800
AARQDPVELL LLSTQERLAA ELQSRRAPLA TMESLSDTES LYSFDSRRSS
810 820 830 840 850
GFRSIRGSPS LHAVLERDEG HLFYIDPAIP EENPSLSSRT ELLAADSLSK
860 870 880 890 900
HSQDTQPLEA ALGSGGVTPE RPPSATIEEE EAAGGSEGGG VAPRGELALH
910 920 930 940 950
NGRLGDSPSP QVLEFAEFID SLFNLDSKSS SFQDLYCMMV PESPTSDYTE
960 970 980 990 1000
GPKSPSQQEI LLRAQFEPNL VPKPPRLFAG SAEPSSGISL SPSFPLSSSG
1010 1020 1030 1040
ELMDLTPTGL SSQECLATDK IRTSTPTGHG ASPTKQDDLV ISAR
Length:1,044
Mass (Da):115,375
Last modified:July 27, 2011 - v2
Checksum:i77E301AE127014F1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti191 – 1911A → P in AAQ17118 (Ref. 1) Curated
Sequence conflicti830 – 8301P → S in AAQ17118 (Ref. 1) Curated
Sequence conflicti945 – 9451T → S in AAQ17118 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY275376 mRNA. Translation: AAQ17118.1.
BC148308 mRNA. Translation: AAI48309.1.
AK129183 mRNA. Translation: BAC97993.1.
CCDSiCCDS29574.1.
RefSeqiNP_932782.2. NM_198114.2.
XP_006527177.1. XM_006527114.2.
UniGeneiMm.329718.

Genome annotation databases

EnsembliENSMUST00000039327; ENSMUSP00000046358; ENSMUSG00000035735.
GeneIDi269060.
KEGGimmu:269060.
UCSCiuc008gpm.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY275376 mRNA. Translation: AAQ17118.1.
BC148308 mRNA. Translation: AAI48309.1.
AK129183 mRNA. Translation: BAC97993.1.
CCDSiCCDS29574.1.
RefSeqiNP_932782.2. NM_198114.2.
XP_006527177.1. XM_006527114.2.
UniGeneiMm.329718.

3D structure databases

ProteinModelPortaliQ6WQJ1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000046358.

Chemistry

ChEMBLiCHEMBL5180.

Protein family/group databases

ESTHERimouse-q6wqj1. Lipase_3.

PTM databases

iPTMnetiQ6WQJ1.
PhosphoSiteiQ6WQJ1.
SwissPalmiQ6WQJ1.

Proteomic databases

MaxQBiQ6WQJ1.
PaxDbiQ6WQJ1.
PRIDEiQ6WQJ1.

Protocols and materials databases

DNASUi269060.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000039327; ENSMUSP00000046358; ENSMUSG00000035735.
GeneIDi269060.
KEGGimmu:269060.
UCSCiuc008gpm.1. mouse.

Organism-specific databases

CTDi747.
MGIiMGI:2677061. Dagla.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG2088. Eukaryota.
ENOG410XRFI. LUCA.
GeneTreeiENSGT00530000063682.
HOGENOMiHOG000015738.
HOVERGENiHBG081435.
InParanoidiQ6WQJ1.
KOiK13806.
OMAiEPRESMQ.
OrthoDBiEOG091G04AM.
TreeFamiTF312928.

Enzyme and pathway databases

ReactomeiR-MMU-426048. Arachidonate production from DAG.

Miscellaneous databases

ChiTaRSiDagla. mouse.
PROiQ6WQJ1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000035735.
CleanExiMM_DAGLA.
ExpressionAtlasiQ6WQJ1. baseline and differential.
GenevisibleiQ6WQJ1. MM.

Family and domain databases

Gene3Di3.40.50.1820. 3 hits.
InterProiIPR029058. AB_hydrolase.
IPR002921. Fungal_lipase-like.
[Graphical view]
PfamiPF01764. Lipase_3. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00120. LIPASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDGLA_MOUSE
AccessioniPrimary (citable) accession number: Q6WQJ1
Secondary accession number(s): A8WFL4, Q6ZQ76
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: July 27, 2011
Last modified: September 7, 2016
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.