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Q6WNW5

- VP3_ROTHD

UniProt

Q6WNW5 - VP3_ROTHD

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Protein
Protein VP3
Gene
N/A
Organism
Rotavirus A (strain Human/United States/DS-1/1976 G2-P1B[4]-I2-R2-C2-M2-A2-N2-T2-E2-H2) (RV-A) (Rotavirus A (strain DS1))
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Multifunctional enzyme involved in mRNA capping. Catalyzes the formation of the 5' cap structure on the viral plus-strand transcripts. Specifically binds to GTP and displays guanylyltransferase and methyltransferase activities. Has affinity for ssRNA but not for dsRNA. Capping activity is non-specific and caps RNAs that initiate with either a G or an A residue. Together with VP1 polymerase, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are capped by VP3 and extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. DLPs probably have an RNA triphosphatase activity as well, whereas open cores don't By similarity.

Catalytic activityi

GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.
S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. RNA binding Source: UniProtKB-KW
  3. mRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
  4. mRNA guanylyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. viral process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

mRNA capping, mRNA processing

Keywords - Ligandi

GTP-binding, Nucleotide-binding, RNA-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Protein VP3
Including the following 2 domains:
mRNA guanylyltransferase (EC:2.7.7.50)
mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56)
OrganismiRotavirus A (strain Human/United States/DS-1/1976 G2-P1B[4]-I2-R2-C2-M2-A2-N2-T2-E2-H2) (RV-A) (Rotavirus A (strain DS1))
Taxonomic identifieri10950 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000001457: Genome

Subcellular locationi

Virion Reviewed prediction
Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging Reviewed prediction.

GO - Cellular componenti

  1. viral nucleocapsid Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 835835Protein VP3
PRO_0000368085Add
BLAST

Interactioni

Subunit structurei

Interacts with VP1 Reviewed prediction. Interacts with VP2 By similarity.

Family & Domainsi

Sequence similaritiesi

Belongs to the rotavirus VP3 family.

Family and domain databases

InterProiIPR011181. VP3_Rotav.
[Graphical view]
PfamiPF06929. Rotavirus_VP3. 1 hit.
[Graphical view]
PIRSFiPIRSF004015. LigT_rotavirus. 1 hit.
PROSITEiPS51589. SAM_MT56_VP3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6WNW5-1 [UniParc]FASTAAdd to Basket

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MKVLALRHSV AQIYADTQVY THDDSKDDYE NAFLISNLTT HNILYLNYSV    50
KTLQILNKSG IAAIEIQKID ELFTLIRCNF TYDYIDDVVY LHDYSYYANN 100
EIRTDQYWVT KTNIEDYLLP GWKLTYVGYN GSDTRGHYNF SFRCQNAATD 150
DDVIIEYIYS NELDFQNFIL KKIKERMTTS LPIARLSNRV FRDKLFKTLS 200
VNHDKVVNVG PRNESMFTFL DHPSIKQFSN GPYLVKDTIK LKQERWLGKR 250
LSQFDIGQYK NMLNVLTTLY QYYDMYHEKP IIYMIGSAPS YWIYDVKQYS 300
DLKFETWDPL DTPYSNLHHK ELFYINDVQK LKDNSILYID IRTDRGNMDW 350
KEWRKVVEGQ TADNLHIAYK YLSTGKAKIC CVKMTAMDVE LPISAKLLHH 400
PTTEIRSEFY LMMDIWDSKN IKRFIPKGVL YSYINNTITE NVFIQQPFKL 450
KTLKNEYVIA LYALSNDLNN REDVVKLINN QKRALITVRI NNTFKDEPKV 500
GFKNIYDWTF LPTDFEMNGS IITSYDGCLG IFGLSISLAS KPTGNNHLFI 550
LSGTDKYFKL DQFANHMSIS RRSHQIRFSE SATSYSGYIF RDLSNNNFNL 600
IGTNVENSVS GHVYNALIYY RYNYSFDLKR WIYLHSTGKA SIEGGKYYEH 650
APIELIYACR SAREFAKLQD DLTVLRYSNE IENYINKVYS ITYADDPNYF 700
IGIKFKNIPY KYNVKVPHLT FGVLNISEQM LPDAIAILKK FKNELFGMDI 750
TTSYTYMLSD EVYVANISGV LSTYFKIYNA FYKEQITFGQ SRMFIPHVTL 800
SFSNEKTVRI DTTKLYIDSI YLRKIKGDTV FDMTE 835
Length:835
Mass (Da):97,903
Last modified:July 5, 2004 - v1
Checksum:iBD63119CD3D9DF14
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY277914 Genomic RNA. Translation: AAQ21041.1.
EF583027 Genomic RNA. Translation: ABU87836.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY277914 Genomic RNA. Translation: AAQ21041.1 .
EF583027 Genomic RNA. Translation: ABU87836.1 .

3D structure databases

ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR011181. VP3_Rotav.
[Graphical view ]
Pfami PF06929. Rotavirus_VP3. 1 hit.
[Graphical view ]
PIRSFi PIRSF004015. LigT_rotavirus. 1 hit.
PROSITEi PS51589. SAM_MT56_VP3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence analysis of the guanylyltransferase (VP3) of group A rotaviruses."
    Cook J.P., McCrae M.A.
    J. Gen. Virol. 85:929-932(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Full genome-based classification of rotaviruses reveals a common origin between human Wa-Like and porcine rotavirus strains and human DS-1-like and bovine rotavirus strains."
    Matthijnssens J., Ciarlet M., Heiman E.M., Arijs I., Delbeke T., McDonald S.M., Palombo E.A., Iturriza-Gomara M., Maes P., Patton J.T., Rahman M., Van Ranst M.
    J. Virol. 82:3204-3219(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiVP3_ROTHD
AccessioniPrimary (citable) accession number: Q6WNW5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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