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Q6WNW5 (VP3_ROTHD) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 28. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Protein VP3

Including the following 2 domains:

  1. mRNA guanylyltransferase
    EC=2.7.7.50
  2. mRNA (guanine-N(7)-)-methyltransferase
    EC=2.1.1.56
OrganismRotavirus A (strain Human/United States/DS-1/1976 G2-P1B[4]-I2-R2-C2-M2-A2-N2-T2-E2-H2) (RV-A) (Rotavirus A (strain DS1)) [Complete proteome]
Taxonomic identifier10950 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length835 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Multifunctional enzyme involved in mRNA capping. Catalyzes the formation of the 5' cap structure on the viral plus-strand transcripts. Specifically binds to GTP and displays guanylyltransferase and methyltransferase activities. Has affinity for ssRNA but not for dsRNA. Capping activity is non-specific and caps RNAs that initiate with either a G or an A residue. Together with VP1 polymerase, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are capped by VP3 and extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. DLPs probably have an RNA triphosphatase activity as well, whereas open cores don't By similarity.

Catalytic activity

GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.

S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

Subunit structure

Interacts with VP1 Potential. Interacts with VP2 By similarity.

Subcellular location

Virion Potential. Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging Potential.

Sequence similarities

Belongs to the rotavirus VP3 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 835835Protein VP3
PRO_0000368085

Sequences

Sequence LengthMass (Da)Tools
Q6WNW5 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: BD63119CD3D9DF14

FASTA83597,903
        10         20         30         40         50         60 
MKVLALRHSV AQIYADTQVY THDDSKDDYE NAFLISNLTT HNILYLNYSV KTLQILNKSG 

        70         80         90        100        110        120 
IAAIEIQKID ELFTLIRCNF TYDYIDDVVY LHDYSYYANN EIRTDQYWVT KTNIEDYLLP 

       130        140        150        160        170        180 
GWKLTYVGYN GSDTRGHYNF SFRCQNAATD DDVIIEYIYS NELDFQNFIL KKIKERMTTS 

       190        200        210        220        230        240 
LPIARLSNRV FRDKLFKTLS VNHDKVVNVG PRNESMFTFL DHPSIKQFSN GPYLVKDTIK 

       250        260        270        280        290        300 
LKQERWLGKR LSQFDIGQYK NMLNVLTTLY QYYDMYHEKP IIYMIGSAPS YWIYDVKQYS 

       310        320        330        340        350        360 
DLKFETWDPL DTPYSNLHHK ELFYINDVQK LKDNSILYID IRTDRGNMDW KEWRKVVEGQ 

       370        380        390        400        410        420 
TADNLHIAYK YLSTGKAKIC CVKMTAMDVE LPISAKLLHH PTTEIRSEFY LMMDIWDSKN 

       430        440        450        460        470        480 
IKRFIPKGVL YSYINNTITE NVFIQQPFKL KTLKNEYVIA LYALSNDLNN REDVVKLINN 

       490        500        510        520        530        540 
QKRALITVRI NNTFKDEPKV GFKNIYDWTF LPTDFEMNGS IITSYDGCLG IFGLSISLAS 

       550        560        570        580        590        600 
KPTGNNHLFI LSGTDKYFKL DQFANHMSIS RRSHQIRFSE SATSYSGYIF RDLSNNNFNL 

       610        620        630        640        650        660 
IGTNVENSVS GHVYNALIYY RYNYSFDLKR WIYLHSTGKA SIEGGKYYEH APIELIYACR 

       670        680        690        700        710        720 
SAREFAKLQD DLTVLRYSNE IENYINKVYS ITYADDPNYF IGIKFKNIPY KYNVKVPHLT 

       730        740        750        760        770        780 
FGVLNISEQM LPDAIAILKK FKNELFGMDI TTSYTYMLSD EVYVANISGV LSTYFKIYNA 

       790        800        810        820        830 
FYKEQITFGQ SRMFIPHVTL SFSNEKTVRI DTTKLYIDSI YLRKIKGDTV FDMTE 

« Hide

References

[1]"Sequence analysis of the guanylyltransferase (VP3) of group A rotaviruses."
Cook J.P., McCrae M.A.
J. Gen. Virol. 85:929-932(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Full genome-based classification of rotaviruses reveals a common origin between human Wa-Like and porcine rotavirus strains and human DS-1-like and bovine rotavirus strains."
Matthijnssens J., Ciarlet M., Heiman E.M., Arijs I., Delbeke T., McDonald S.M., Palombo E.A., Iturriza-Gomara M., Maes P., Patton J.T., Rahman M., Van Ranst M.
J. Virol. 82:3204-3219(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY277914 Genomic RNA. Translation: AAQ21041.1.
EF583027 Genomic RNA. Translation: ABU87836.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR011181. VP3_Rotav.
[Graphical view]
PfamPF06929. Rotavirus_VP3. 1 hit.
[Graphical view]
PIRSFPIRSF004015. LigT_rotavirus. 1 hit.
PROSITEPS51589. SAM_MT56_VP3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameVP3_ROTHD
AccessionPrimary (citable) accession number: Q6WNW5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families