SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q6WNV8

- VP3_ROTP5

UniProt

Q6WNV8 - VP3_ROTP5

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Protein VP3
Gene
N/A
Organism
Rotavirus A (strain Pig/United States/OSU/1977 G5-P9[7]-I5-R1-C1-M1-A1-N1-T1-E1-H1) (RV-A) (Rotavirus A (strain Ohio State University))
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Multifunctional enzyme involved in mRNA capping. Catalyzes the formation of the 5' cap structure on the viral plus-strand transcripts. Specifically binds to GTP and displays guanylyltransferase and methyltransferase activities. Has affinity for ssRNA but not for dsRNA. Capping activity is non-specific and caps RNAs that initiate with either a G or an A residue. Together with VP1 polymerase, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are capped by VP3 and extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. DLPs probably have an RNA triphosphatase activity as well, whereas open cores don't By similarity.

Catalytic activityi

GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.
S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. RNA binding Source: UniProtKB-KW
  3. mRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
  4. mRNA guanylyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. viral process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

mRNA capping, mRNA processing

Keywords - Ligandi

GTP-binding, Nucleotide-binding, RNA-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Protein VP3
Including the following 2 domains:
mRNA guanylyltransferase (EC:2.7.7.50)
mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56)
OrganismiRotavirus A (strain Pig/United States/OSU/1977 G5-P9[7]-I5-R1-C1-M1-A1-N1-T1-E1-H1) (RV-A) (Rotavirus A (strain Ohio State University))
Taxonomic identifieri10915 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
Virus hostiSus scrofa (Pig) [TaxID: 9823]

Subcellular locationi

Virion Reviewed prediction
Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging Reviewed prediction.

GO - Cellular componenti

  1. viral nucleocapsid Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 835835Protein VP3
PRO_0000368088Add
BLAST

Interactioni

Subunit structurei

Interacts with VP1 Reviewed prediction. Interacts with VP2 By similarity.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi270 – 2734Poly-Tyr

Sequence similaritiesi

Belongs to the rotavirus VP3 family.

Family and domain databases

InterProiIPR011181. VP3_Rotav.
[Graphical view]
PfamiPF06929. Rotavirus_VP3. 1 hit.
[Graphical view]
PIRSFiPIRSF004015. LigT_rotavirus. 1 hit.
PROSITEiPS51589. SAM_MT56_VP3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6WNV8-1 [UniParc]FASTAAdd to Basket

« Hide

MKVLALRHSV AQVYADTQTY VHDDSKDEYE NAFLISNLTT HNILYINYSV    50
KTLKILNKSG IAAVEIQSPD ELFALIRCNF TYDYENNTVY LHDYSYYTNN 100
EIRTDQHWVT KTDIIDYLLP GWKLTYVGYN GKDTRGHYNF SFTCQNAATD 150
DDIIIEYIYS SELDFQTFLL RKIKERMTTS LPIARLSNRV FRDKLFPSIV 200
NIHKRVVNVG PRNESMFTFL NFPTIKQFSN GAYLVKHTIK LKQERWLGKR 250
VSQFDIGQYK NMLNVVTTIY YYYNLHSSKP VIYMLGSAPS YWIYDIRQYS 300
DFTFETWDPL DTPYSTTHHK ELFFDKDVTK LKDNSILYID IRTDRGNMDW 350
KEWRKVVEQQ TVSNLSIAYK YLSTGKAKVC CVKLTAMDLE LPITAKLLHH 400
PTTEIRSEFY AILDVWDIST IKRFVPKGVF YAFINNVTTE NVFIQPPFKL 450
RTLPNDYIVA LYALSNDFNP RQDIINLINK QKQSLITVRI NNTFKDEPKV 500
NFKNIYDWTF LPTDFEIKDS IITSYDGCLG IFGLSTSLSS KPTGNNHLFI 550
INGTDKYYKL DQYANHMGIS RRSHQVRFSE SATSYSGYIF RDLSNNNFNL 600
IGTNIENSVS GHVYNALIYY RYNYAFDLKR WIYLHSIGEV TVEGGKYYEH 650
APIELIYACR SAKEFAALQD DLTVLRYANE IEGYINKVYS ITYADDPNYF 700
IGVRFDSIPY EYDVKVPHLT FGVLFISDNM IHDVITVLKK MKTELFKMEV 750
STSYTYMLSD GIHVANASGV LSTYFKLYNM FYRNHITFGQ SRMFIPHITL 800
SFSNKRTVRI ENTRLKINSI YLRKIKGETV FDMSE 835
Length:835
Mass (Da):97,663
Last modified:July 5, 2004 - v1
Checksum:i89207782ACE2733A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY277921 Genomic RNA. Translation: AAQ21048.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY277921 Genomic RNA. Translation: AAQ21048.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR011181. VP3_Rotav.
[Graphical view ]
Pfami PF06929. Rotavirus_VP3. 1 hit.
[Graphical view ]
PIRSFi PIRSF004015. LigT_rotavirus. 1 hit.
PROSITEi PS51589. SAM_MT56_VP3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence analysis of the guanylyltransferase (VP3) of group A rotaviruses."
    Cook J.P., McCrae M.A.
    J. Gen. Virol. 85:929-932(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiVP3_ROTP5
AccessioniPrimary (citable) accession number: Q6WNV8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi