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Q6WNV8

- VP3_ROTP5

UniProt

Q6WNV8 - VP3_ROTP5

Protein

Protein VP3

Gene
N/A
Organism
Rotavirus A (strain Pig/United States/OSU/1977 G5-P9[7]-I5-R1-C1-M1-A1-N1-T1-E1-H1) (RV-A) (Rotavirus A (strain Ohio State University))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 26 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Multifunctional enzyme involved in mRNA capping. Catalyzes the formation of the 5' cap structure on the viral plus-strand transcripts. Specifically binds to GTP and displays guanylyltransferase and methyltransferase activities. Has affinity for ssRNA but not for dsRNA. Capping activity is non-specific and caps RNAs that initiate with either a G or an A residue. Together with VP1 polymerase, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are capped by VP3 and extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. DLPs probably have an RNA triphosphatase activity as well, whereas open cores don't By similarity.By similarity

    Catalytic activityi

    GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.
    S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-KW
    2. mRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
    3. mRNA guanylyltransferase activity Source: UniProtKB-EC
    4. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. viral process Source: InterPro

    Keywords - Molecular functioni

    Methyltransferase, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    mRNA capping, mRNA processing

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding, RNA-binding, S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein VP3
    Including the following 2 domains:
    mRNA guanylyltransferase (EC:2.7.7.50)
    mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56)
    OrganismiRotavirus A (strain Pig/United States/OSU/1977 G5-P9[7]-I5-R1-C1-M1-A1-N1-T1-E1-H1) (RV-A) (Rotavirus A (strain Ohio State University))
    Taxonomic identifieri10915 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
    Virus hostiSus scrofa (Pig) [TaxID: 9823]

    Subcellular locationi

    Virion Curated
    Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging Potential.Curated

    GO - Cellular componenti

    1. viral nucleocapsid Source: InterPro

    Keywords - Cellular componenti

    Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 835835Protein VP3PRO_0000368088Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts with VP1 Potential. Interacts with VP2 By similarity.By similarityCurated

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi270 – 2734Poly-Tyr

    Sequence similaritiesi

    Belongs to the rotavirus VP3 family.Curated

    Family and domain databases

    InterProiIPR011181. VP3_Rotav.
    [Graphical view]
    PfamiPF06929. Rotavirus_VP3. 1 hit.
    [Graphical view]
    PIRSFiPIRSF004015. LigT_rotavirus. 1 hit.
    PROSITEiPS51589. SAM_MT56_VP3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6WNV8-1 [UniParc]FASTAAdd to Basket

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    MKVLALRHSV AQVYADTQTY VHDDSKDEYE NAFLISNLTT HNILYINYSV    50
    KTLKILNKSG IAAVEIQSPD ELFALIRCNF TYDYENNTVY LHDYSYYTNN 100
    EIRTDQHWVT KTDIIDYLLP GWKLTYVGYN GKDTRGHYNF SFTCQNAATD 150
    DDIIIEYIYS SELDFQTFLL RKIKERMTTS LPIARLSNRV FRDKLFPSIV 200
    NIHKRVVNVG PRNESMFTFL NFPTIKQFSN GAYLVKHTIK LKQERWLGKR 250
    VSQFDIGQYK NMLNVVTTIY YYYNLHSSKP VIYMLGSAPS YWIYDIRQYS 300
    DFTFETWDPL DTPYSTTHHK ELFFDKDVTK LKDNSILYID IRTDRGNMDW 350
    KEWRKVVEQQ TVSNLSIAYK YLSTGKAKVC CVKLTAMDLE LPITAKLLHH 400
    PTTEIRSEFY AILDVWDIST IKRFVPKGVF YAFINNVTTE NVFIQPPFKL 450
    RTLPNDYIVA LYALSNDFNP RQDIINLINK QKQSLITVRI NNTFKDEPKV 500
    NFKNIYDWTF LPTDFEIKDS IITSYDGCLG IFGLSTSLSS KPTGNNHLFI 550
    INGTDKYYKL DQYANHMGIS RRSHQVRFSE SATSYSGYIF RDLSNNNFNL 600
    IGTNIENSVS GHVYNALIYY RYNYAFDLKR WIYLHSIGEV TVEGGKYYEH 650
    APIELIYACR SAKEFAALQD DLTVLRYANE IEGYINKVYS ITYADDPNYF 700
    IGVRFDSIPY EYDVKVPHLT FGVLFISDNM IHDVITVLKK MKTELFKMEV 750
    STSYTYMLSD GIHVANASGV LSTYFKLYNM FYRNHITFGQ SRMFIPHITL 800
    SFSNKRTVRI ENTRLKINSI YLRKIKGETV FDMSE 835
    Length:835
    Mass (Da):97,663
    Last modified:July 5, 2004 - v1
    Checksum:i89207782ACE2733A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY277921 Genomic RNA. Translation: AAQ21048.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY277921 Genomic RNA. Translation: AAQ21048.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR011181. VP3_Rotav.
    [Graphical view ]
    Pfami PF06929. Rotavirus_VP3. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF004015. LigT_rotavirus. 1 hit.
    PROSITEi PS51589. SAM_MT56_VP3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of the guanylyltransferase (VP3) of group A rotaviruses."
      Cook J.P., McCrae M.A.
      J. Gen. Virol. 85:929-932(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiVP3_ROTP5
    AccessioniPrimary (citable) accession number: Q6WNV8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 26 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Multifunctional enzyme

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3