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Q6WNV7

- VP3_ROTEL

UniProt

Q6WNV7 - VP3_ROTEL

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Protein

Protein VP3

Gene
N/A
Organism
Rotavirus A (isolate Equine/United Kingdom/L338/1988 G13-P12[18]-I6-Rx-Cx-M6-A6-Nx-Tx-Ex-Hx) (RV-A)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Multifunctional enzyme involved in mRNA capping. Catalyzes the formation of the 5' cap structure on the viral plus-strand transcripts. Specifically binds to GTP and displays guanylyltransferase and methyltransferase activities. Has affinity for ssRNA but not for dsRNA. Capping activity is non-specific and caps RNAs that initiate with either a G or an A residue. Together with VP1 polymerase, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are capped by VP3 and extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. DLPs probably have an RNA triphosphatase activity as well, whereas open cores don't By similarity.

Catalytic activityi

GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.
S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. mRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
  3. mRNA guanylyltransferase activity Source: UniProtKB-EC
  4. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. viral process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

mRNA capping, mRNA processing

Keywords - Ligandi

GTP-binding, Nucleotide-binding, RNA-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Protein VP3
Including the following 2 domains:
mRNA guanylyltransferase (EC:2.7.7.50)
mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56)
OrganismiRotavirus A (isolate Equine/United Kingdom/L338/1988 G13-P12[18]-I6-Rx-Cx-M6-A6-Nx-Tx-Ex-Hx) (RV-A)
Taxonomic identifieri36441 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiEquus caballus (Horse) [TaxID: 9796]

Subcellular locationi

Virion Reviewed prediction
Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging Reviewed prediction.

GO - Cellular componenti

  1. viral nucleocapsid Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 835835Protein VP3PRO_0000368066Add
BLAST

Interactioni

Subunit structurei

Interacts with VP1 Reviewed prediction. Interacts with VP2 By similarity.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi270 – 2734Poly-Tyr

Sequence similaritiesi

Belongs to the rotavirus VP3 family.

Family and domain databases

InterProiIPR011181. VP3_Rotav.
[Graphical view]
PfamiPF06929. Rotavirus_VP3. 1 hit.
[Graphical view]
PIRSFiPIRSF004015. LigT_rotavirus. 1 hit.
PROSITEiPS51589. SAM_MT56_VP3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6WNV7-1 [UniParc]FASTAAdd to Basket

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MKVLALRHSV TQVYADTQTY LHDDMKDDYE NAFLISNLTV HNILYLNYSL    50
KTLEILNKSG IAAVEVQGIE ELLALIRCNF TYDYLNNVVF LHDYSYYTNN 100
EIRTDQHWIT KTDIENYLLP GWKLTYVGYN GKSTRGHYNF SFTCQNAATD 150
DDIIIEYIYS NELDFQNFML SKIKERMTTA LPIARLSNRV FRDKLFQSLS 200
LNNDKIVNVG PRNESMFTFL KFPSIKQFSD GPYLVKDTIK LKQERWLGKR 250
VSQFDIGQYK NMLNVITTIY YYYNLYAEKP IVYMLGSAPS YWIYDIKQYS 300
EFVFETWDPL DTPYSNMHHK ELFFEKDTIK LKDNSILYID IRTDRGDIDW 350
KEWRKIVKEQ TLSNLNIAYK YLSTGKSKVC CVKMTAMDIE LPISAKLLHH 400
PTTEIRSEFY MIVDLSDFKN IKRFVPKGVL YSFINNITTE NVFIQHPFKL 450
RKMKNEYIVA LYALSNDFNN RENVIKLINE QEKSLITVRL NNTFKDEPKI 500
GFKNIYDWTF LPTDFNTRNS IITSYDGCIG MFGLSISLSS KPTGNNHIFI 550
LNGTDKYEMI DQFANHMGIS RRSHQIRFSE SATSYSGYIF RDLSNNNFNL 600
IGTNVENSVS GHVYNAIIYF RYNYSFDLKR WIHLHSVDKV KIEGGRYYEH 650
APIELIYACR SAKEFAKMQD DLTTLRYANE IERYINKVYS IVYADDPNYF 700
IGIKFVSIPY KYDVKVPHLT FGVLHISDNM IPDVISILNQ MKVELFKMNI 750
TTSYTYMLSD GIHVANVSGV LLTYFKIYNV FYKKQITFGQ SRMFIPHITL 800
SFKTNKTIRI NITKLKIESI YLRKIKGETV FAMTE 835
Length:835
Mass (Da):98,047
Last modified:July 5, 2004 - v1
Checksum:iD7B1FC83AF2FA14F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY277922 Genomic RNA. Translation: AAQ21049.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY277922 Genomic RNA. Translation: AAQ21049.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR011181. VP3_Rotav.
[Graphical view ]
Pfami PF06929. Rotavirus_VP3. 1 hit.
[Graphical view ]
PIRSFi PIRSF004015. LigT_rotavirus. 1 hit.
PROSITEi PS51589. SAM_MT56_VP3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence analysis of the guanylyltransferase (VP3) of group A rotaviruses."
    Cook J.P., McCrae M.A.
    J. Gen. Virol. 85:929-932(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiVP3_ROTEL
AccessioniPrimary (citable) accession number: Q6WNV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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