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Q6WNV6

- VP3_ROTCC

UniProt

Q6WNV6 - VP3_ROTCC

Protein

Protein VP3

Gene
N/A
Organism
Rotavirus A (strain Chicken/Ireland/Ch2/1979 G7-Px[x]-Ix-Rx-Cx-M4-Ax-Nx-Tx-Ex-Hx) (RV-A) (Rotavirus A (strain Ch-2))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 26 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Multifunctional enzyme involved in mRNA capping. Catalyzes the formation of the 5' cap structure on the viral plus-strand transcripts. Specifically binds to GTP and displays guanylyltransferase and methyltransferase activities. Has affinity for ssRNA but not for dsRNA. Capping activity is non-specific and caps RNAs that initiate with either a G or an A residue. Together with VP1 polymerase, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are capped by VP3 and extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. DLPs probably have an RNA triphosphatase activity as well, whereas open cores don't By similarity.By similarity

    Catalytic activityi

    GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.
    S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-KW
    2. mRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
    3. mRNA guanylyltransferase activity Source: UniProtKB-EC
    4. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. viral process Source: InterPro

    Keywords - Molecular functioni

    Methyltransferase, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    mRNA capping, mRNA processing

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding, RNA-binding, S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein VP3
    Including the following 2 domains:
    mRNA guanylyltransferase (EC:2.7.7.50)
    mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56)
    OrganismiRotavirus A (strain Chicken/Ireland/Ch2/1979 G7-Px[x]-Ix-Rx-Cx-M4-Ax-Nx-Tx-Ex-Hx) (RV-A) (Rotavirus A (strain Ch-2))
    Taxonomic identifieri31589 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
    Virus hostiGallus gallus (Chicken) [TaxID: 9031]

    Subcellular locationi

    Virion Curated
    Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging Potential.Curated

    GO - Cellular componenti

    1. viral nucleocapsid Source: InterPro

    Keywords - Cellular componenti

    Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 829829Protein VP3PRO_0000368072Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts with VP1 Potential. Interacts with VP2 By similarity.By similarityCurated

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi267 – 2704Poly-Tyr

    Sequence similaritiesi

    Belongs to the rotavirus VP3 family.Curated

    Family and domain databases

    InterProiIPR011181. VP3_Rotav.
    [Graphical view]
    PfamiPF06929. Rotavirus_VP3. 1 hit.
    [Graphical view]
    PIRSFiPIRSF004015. LigT_rotavirus. 1 hit.
    PROSITEiPS51589. SAM_MT56_VP3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6WNV6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKVIALRRDL VSSYADTQVY EHDVDKDYYE NAYLISNITA HNVLYVDYSI    50
    QVIEILNKSG IASLIAINKD KLEILIKSNY TYDYYKNIVY LHDYSYYNLN 100
    ELRTDQYWLT TTNIEEYILP GWKLTYVGQL GIKTRGHYTY SFICQNTATD 150
    DDIIYDYIYS NEVDFLPFLL QALNKRLTTA VNFHRLSNRI FREYLYSKVP 200
    KDTINIGPRN ESMFTLLRYP YITNYAANEF KVSDLIRLTQ EKWIGAENSQ 250
    FDIGQFKNMC NVLSTIYYYY NKYHAYPRIY MLGSAPSYWL YDLLQVYNFD 300
    IETWDPLDTP FSKRHHKAMF AISDTEKLSD NSILYIDIRS DRNGADWREW 350
    RQRVEDETKI NLEIMRKYLQ RGKTRICCCK ITAMDIELPA TSILLHFPTT 400
    KIQSECYVIC TQEMLQDKKR FVPKGAFYSF INNTKTDNVF VSPVYKVKPT 450
    NKFVVALYSL SNENNDRDKV IDFTQKQKRG IITLRMNNTF NYEYRLQFKS 500
    TYDYLYLPSE VSREGTIVTS YDGYIAMHNL SLSLESKATG NNHLFISFSD 550
    ANYGQIDSYC THMGISRRSH SVRFSEAATT LSGYMFRDIT NGKFNLINTN 600
    VENAVSGHVY NALVYFRYNY RFDLLRWINL HAKDEVMIQG GRYYEHAPPE 650
    LLYACQSALV FAKLQNDLTL IEYVNSVNRY ILNKYNLKYA DDPNYYIHID 700
    FVDLPFKYTV PDPHLTGGLL FLNDFDIQNT ITILRSVKED LIALNLLTQY 750
    TYMLTDSIYV ANVDGYLQFY YKLYMKFYRK QIVFGQSRMF LPHITLSKEK 800
    KKPVRIDATK LVIKSIKLRK IKSDVEYCI 829
    Length:829
    Mass (Da):97,789
    Last modified:July 5, 2004 - v1
    Checksum:iA45EE6B3F66379E1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY277923 Genomic RNA. Translation: AAQ21050.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY277923 Genomic RNA. Translation: AAQ21050.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR011181. VP3_Rotav.
    [Graphical view ]
    Pfami PF06929. Rotavirus_VP3. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF004015. LigT_rotavirus. 1 hit.
    PROSITEi PS51589. SAM_MT56_VP3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of the guanylyltransferase (VP3) of group A rotaviruses."
      Cook J.P., McCrae M.A.
      J. Gen. Virol. 85:929-932(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiVP3_ROTCC
    AccessioniPrimary (citable) accession number: Q6WNV6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 26 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Multifunctional enzyme

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3