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Q6WNV6

- VP3_ROTCC

UniProt

Q6WNV6 - VP3_ROTCC

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Protein

Protein VP3

Gene
N/A
Organism
Rotavirus A (strain Chicken/Ireland/Ch2/1979 G7-Px[x]-Ix-Rx-Cx-M4-Ax-Nx-Tx-Ex-Hx) (RV-A) (Rotavirus A (strain Ch-2))
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Multifunctional enzyme involved in mRNA capping. Catalyzes the formation of the 5' cap structure on the viral plus-strand transcripts. Specifically binds to GTP and displays guanylyltransferase and methyltransferase activities. Has affinity for ssRNA but not for dsRNA. Capping activity is non-specific and caps RNAs that initiate with either a G or an A residue. Together with VP1 polymerase, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are capped by VP3 and extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. DLPs probably have an RNA triphosphatase activity as well, whereas open cores don't By similarity.

Catalytic activityi

GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.
S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. mRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
  3. mRNA guanylyltransferase activity Source: UniProtKB-EC
  4. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. viral process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

mRNA capping, mRNA processing

Keywords - Ligandi

GTP-binding, Nucleotide-binding, RNA-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Protein VP3
Including the following 2 domains:
mRNA guanylyltransferase (EC:2.7.7.50)
mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56)
OrganismiRotavirus A (strain Chicken/Ireland/Ch2/1979 G7-Px[x]-Ix-Rx-Cx-M4-Ax-Nx-Tx-Ex-Hx) (RV-A) (Rotavirus A (strain Ch-2))
Taxonomic identifieri31589 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiGallus gallus (Chicken) [TaxID: 9031]

Subcellular locationi

Virion Reviewed prediction
Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging Reviewed prediction.

GO - Cellular componenti

  1. viral nucleocapsid Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 829829Protein VP3PRO_0000368072Add
BLAST

Interactioni

Subunit structurei

Interacts with VP1 Reviewed prediction. Interacts with VP2 By similarity.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi267 – 2704Poly-Tyr

Sequence similaritiesi

Belongs to the rotavirus VP3 family.

Family and domain databases

InterProiIPR011181. VP3_Rotav.
[Graphical view]
PfamiPF06929. Rotavirus_VP3. 1 hit.
[Graphical view]
PIRSFiPIRSF004015. LigT_rotavirus. 1 hit.
PROSITEiPS51589. SAM_MT56_VP3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6WNV6-1 [UniParc]FASTAAdd to Basket

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MKVIALRRDL VSSYADTQVY EHDVDKDYYE NAYLISNITA HNVLYVDYSI    50
QVIEILNKSG IASLIAINKD KLEILIKSNY TYDYYKNIVY LHDYSYYNLN 100
ELRTDQYWLT TTNIEEYILP GWKLTYVGQL GIKTRGHYTY SFICQNTATD 150
DDIIYDYIYS NEVDFLPFLL QALNKRLTTA VNFHRLSNRI FREYLYSKVP 200
KDTINIGPRN ESMFTLLRYP YITNYAANEF KVSDLIRLTQ EKWIGAENSQ 250
FDIGQFKNMC NVLSTIYYYY NKYHAYPRIY MLGSAPSYWL YDLLQVYNFD 300
IETWDPLDTP FSKRHHKAMF AISDTEKLSD NSILYIDIRS DRNGADWREW 350
RQRVEDETKI NLEIMRKYLQ RGKTRICCCK ITAMDIELPA TSILLHFPTT 400
KIQSECYVIC TQEMLQDKKR FVPKGAFYSF INNTKTDNVF VSPVYKVKPT 450
NKFVVALYSL SNENNDRDKV IDFTQKQKRG IITLRMNNTF NYEYRLQFKS 500
TYDYLYLPSE VSREGTIVTS YDGYIAMHNL SLSLESKATG NNHLFISFSD 550
ANYGQIDSYC THMGISRRSH SVRFSEAATT LSGYMFRDIT NGKFNLINTN 600
VENAVSGHVY NALVYFRYNY RFDLLRWINL HAKDEVMIQG GRYYEHAPPE 650
LLYACQSALV FAKLQNDLTL IEYVNSVNRY ILNKYNLKYA DDPNYYIHID 700
FVDLPFKYTV PDPHLTGGLL FLNDFDIQNT ITILRSVKED LIALNLLTQY 750
TYMLTDSIYV ANVDGYLQFY YKLYMKFYRK QIVFGQSRMF LPHITLSKEK 800
KKPVRIDATK LVIKSIKLRK IKSDVEYCI 829
Length:829
Mass (Da):97,789
Last modified:July 5, 2004 - v1
Checksum:iA45EE6B3F66379E1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY277923 Genomic RNA. Translation: AAQ21050.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY277923 Genomic RNA. Translation: AAQ21050.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR011181. VP3_Rotav.
[Graphical view ]
Pfami PF06929. Rotavirus_VP3. 1 hit.
[Graphical view ]
PIRSFi PIRSF004015. LigT_rotavirus. 1 hit.
PROSITEi PS51589. SAM_MT56_VP3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence analysis of the guanylyltransferase (VP3) of group A rotaviruses."
    Cook J.P., McCrae M.A.
    J. Gen. Virol. 85:929-932(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiVP3_ROTCC
AccessioniPrimary (citable) accession number: Q6WNV6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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