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Protein

Chordin-like protein 2

Gene

CHRDL2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May inhibit BMPs activity by blocking their interaction with their receptors. Has a negative regulator effect on the cartilage formation/regeneration from immature mesenchymal cells, by preventing or reducing the rate of matrix accumulation (By similarity). Implicated in tumor angiogenesis. May play a role during myoblast and osteoblast differentiation, and maturation.By similarity2 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Chondrogenesis, Differentiation, Osteogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Chordin-like protein 2
Alternative name(s):
Breast tumor novel factor 1
Short name:
BNF-1
Chordin-related protein 2
Gene namesi
Name:CHRDL2
Synonyms:BNF1, CHL2
ORF Names:UNQ765/PRO1557
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:24168. CHRDL2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • extracellular space Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Secreted

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000054938.
PharmGKBiPA134883081.

Polymorphism and mutation databases

DMDMi62900089.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 251 PublicationAdd BLAST25
ChainiPRO_000000537126 – 429Chordin-like protein 2Add BLAST404

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi114N-linked (GlcNAc...)1 Publication1
Modified residuei182Phosphoserine; by FAM20C1 Publication1

Post-translational modificationi

Phosphorylated by FAM20C in the extracellular medium.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PeptideAtlasiQ6WN34.
PRIDEiQ6WN34.

PTM databases

iPTMnetiQ6WN34.
PhosphoSitePlusiQ6WN34.

Expressioni

Tissue specificityi

Highly expressed in uterus. Moderately expressed in heart, liver, prostate, testis and ovary. Weakly expressed in skeletal muscle, kidney, spleen, small intestine and colon. Expressed in the secretory epithelial cells of uterine endometrium, fallopian tubes, endocervical glands, bladder and prostate, as well as the transitional epithelium of the urinary bladder, and in bone osteoblasts (at protein level). In normal cartilage, expression was confined in a few chondrocytes in the superficial zone as well as in the middle zone. In diseased cartilage coming from osteoarthritic patients, expression was limited to the middle zone of chondrocytes. Isoform 1 and isoform 2 are expressed in fetal cerebellum and heart, while only isoform 2 is detected in fetal spleen. Isoform 2 present in plasma.3 Publications

Inductioni

Up-regulated in breast tumors but also in lung and colon tumors.1 Publication

Gene expression databases

BgeeiENSG00000054938.
CleanExiHS_CHRDL2.
ExpressionAtlasiQ6WN34. baseline and differential.
GenevisibleiQ6WN34. HS.

Organism-specific databases

HPAiHPA021771.

Interactioni

Subunit structurei

Interacts with GDF5 (By similarity). May interact with BMP2, BMP4, BMP5, BMP6, BMP7 and INHBA.By similarity2 Publications

Protein-protein interaction databases

BioGridi117395. 1 interactor.

Structurei

3D structure databases

ProteinModelPortaliQ6WN34.
SMRiQ6WN34.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 96VWFC 1PROSITE-ProRule annotationAdd BLAST66
Domaini109 – 175VWFC 2PROSITE-ProRule annotationAdd BLAST67
Domaini250 – 315VWFC 3PROSITE-ProRule annotationAdd BLAST66

Sequence similaritiesi

Contains 3 VWFC domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

GeneTreeiENSGT00730000110792.
HOGENOMiHOG000049211.
HOVERGENiHBG051113.
InParanoidiQ6WN34.
KOiK17280.
OMAiVKGIFHL.
OrthoDBiEOG091G0WK8.
PhylomeDBiQ6WN34.
TreeFamiTF106451.

Family and domain databases

InterProiIPR001007. VWF_dom.
[Graphical view]
PfamiPF00093. VWC. 3 hits.
[Graphical view]
SMARTiSM00214. VWC. 3 hits.
[Graphical view]
PROSITEiPS01208. VWFC_1. 3 hits.
PS50184. VWFC_2. 3 hits.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Five additional mRNAs also exist. Differential expression of isoforms was observed during myoblast and osteoblast differentiation and maturation.
Isoform 1 (identifier: Q6WN34-1) [UniParc]FASTAAdd to basket
Also known as: I

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVPEVRVLSS LLGLALLWFP LDSHARARPD MFCLFHGKRY SPGESWHPYL
60 70 80 90 100
EPQGLMYCLR CTCSEGAHVS CYRLHCPPVH CPQPVTEPQQ CCPKCVEPHT
110 120 130 140 150
PSGLRAPPKS CQHNGTMYQH GEIFSAHELF PSRLPNQCVL CSCTEGQIYC
160 170 180 190 200
GLTTCPEPGC PAPLPLPDSC CQACKDEASE QSDEEDSVQS LHGVRHPQDP
210 220 230 240 250
CSSDAGRKRG PGTPAPTGLS APLSFIPRHF RPKGAGSTTV KIVLKEKHKK
260 270 280 290 300
ACVHGGKTYS HGEVWHPAFR AFGPLPCILC TCEDGRQDCQ RVTCPTEYPC
310 320 330 340 350
RHPEKVAGKC CKICPEDKAD PGHSEISSTR CPKAPGRVLV HTSVSPSPDN
360 370 380 390 400
LRRFALEHEA SDLVEIYLWK LVKGIFHLTQ IKKVRKQDFQ KEAQHFRLLA
410 420
GPHEGHWNVF LAQTLELKVT ASPDKVTKT
Length:429
Mass (Da):47,495
Last modified:July 5, 2004 - v1
Checksum:iA8111F26B2EF96A4
GO
Isoform 2 (identifier: Q6WN34-2) [UniParc]FASTAAdd to basket
Also known as: II

The sequence of this isoform differs from the canonical sequence as follows:
     374-426: GIFHLTQIKK...LKVTASPDKV → DEETEAQRGE...GQSRQSDQDI

Show »
Length:451
Mass (Da):49,675
Checksum:iCFBF0BFA7D58CFE3
GO
Isoform 3 (identifier: Q6WN34-3) [UniParc]FASTAAdd to basket
Also known as: VII

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: Missing.
     21-28: LDSHARAR → MALVGLPG
     66-162: GAHVSCYRLH...TTCPEPGCPA → NLTLPLDSGP...KRTVCSRSMG
     163-429: Missing.

Show »
Length:142
Mass (Da):15,136
Checksum:i66564AA513E1D2E7
GO
Isoform 4 (identifier: Q6WN34-4) [UniParc]FASTAAdd to basket
Also known as: VIII

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: Missing.
     21-28: LDSHARAR → MALVGLPG
     145-162: EGQIYCGLTTCPEPGCPA → MRQVSNRMKRTVCSRSMG
     163-429: Missing.

Show »
Length:142
Mass (Da):15,922
Checksum:i577245066155CBC1
GO
Isoform 5 (identifier: Q6WN34-5) [UniParc]FASTAAdd to basket
Also known as: IX

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: Missing.
     21-28: LDSHARAR → MALVGLPG
     66-113: GAHVSCYRLH...LRAPPKSCQH → NLTLPLDSGP...PTSVSSAAAQ
     114-429: Missing.

Show »
Length:93
Mass (Da):9,846
Checksum:i9DFEC54E7C1EF6CE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti187S → R in AX140199 (PubMed:15094188).Curated1
Sequence conflicti231R → I in AX140199 (PubMed:15094188).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_055651335P → L.Corresponds to variant rs35903991dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0135121 – 20Missing in isoform 3, isoform 4 and isoform 5. 1 PublicationAdd BLAST20
Alternative sequenceiVSP_01351321 – 28LDSHARAR → MALVGLPG in isoform 3, isoform 4 and isoform 5. 1 Publication8
Alternative sequenceiVSP_01351466 – 162GAHVS…PGCPA → NLTLPLDSGPHQSPASTTGP CTNTERSSVPMSCSPPACPT SVSSAAAQRARSTAASQPAP NQAAQHPSRCQTPAAKPAKM RQVSNRMKRTVCSRSMG in isoform 3. 1 PublicationAdd BLAST97
Alternative sequenceiVSP_01351566 – 113GAHVS…KSCQH → NLTLPLDSGPHQSPASTTGP CTNTERSSVPMSCSPPACPT SVSSAAAQ in isoform 5. 1 PublicationAdd BLAST48
Alternative sequenceiVSP_013516114 – 429Missing in isoform 5. 1 PublicationAdd BLAST316
Alternative sequenceiVSP_013517145 – 162EGQIY…PGCPA → MRQVSNRMKRTVCSRSMG in isoform 4. 1 PublicationAdd BLAST18
Alternative sequenceiVSP_013518163 – 429Missing in isoform 3 and isoform 4. 1 PublicationAdd BLAST267
Alternative sequenceiVSP_013519374 – 426GIFHL…SPDKV → DEETEAQRGEVPGPRPHSQN LPLDSDQESQEARLPERGTA LPTARWPPRRSLERLPSPDP GAEGHGQSRQSDQDI in isoform 2. 3 PublicationsAdd BLAST53

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY163868 mRNA. Translation: AAO31809.1.
AY279090 mRNA. Translation: AAQ19179.1.
AY279092 mRNA. Translation: AAQ19181.1.
AY279093 mRNA. Translation: AAQ19182.1.
AY279094 mRNA. Translation: AAQ19183.1.
AX140199 mRNA. No translation available.
AY358522 mRNA. Translation: AAQ88886.1.
BC142623 mRNA. Translation: AAI42624.1.
CCDSiCCDS60893.1. [Q6WN34-1]
CCDS8234.1. [Q6WN34-2]
RefSeqiNP_001265402.1. NM_001278473.2. [Q6WN34-1]
NP_001291319.1. NM_001304390.1.
NP_001291320.1. NM_001304391.1.
NP_001291344.1. NM_001304415.1.
NP_001291345.1. NM_001304416.1.
NP_001291346.1. NM_001304417.1.
NP_056239.3. NM_015424.5. [Q6WN34-2]
UniGeneiHs.432379.

Genome annotation databases

EnsembliENST00000263671; ENSP00000263671; ENSG00000054938. [Q6WN34-2]
ENST00000376332; ENSP00000365510; ENSG00000054938. [Q6WN34-1]
ENST00000528471; ENSP00000434589; ENSG00000054938. [Q6WN34-5]
ENST00000534276; ENSP00000432055; ENSG00000054938. [Q6WN34-4]
GeneIDi25884.
KEGGihsa:25884.
UCSCiuc001ovh.5. human. [Q6WN34-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY163868 mRNA. Translation: AAO31809.1.
AY279090 mRNA. Translation: AAQ19179.1.
AY279092 mRNA. Translation: AAQ19181.1.
AY279093 mRNA. Translation: AAQ19182.1.
AY279094 mRNA. Translation: AAQ19183.1.
AX140199 mRNA. No translation available.
AY358522 mRNA. Translation: AAQ88886.1.
BC142623 mRNA. Translation: AAI42624.1.
CCDSiCCDS60893.1. [Q6WN34-1]
CCDS8234.1. [Q6WN34-2]
RefSeqiNP_001265402.1. NM_001278473.2. [Q6WN34-1]
NP_001291319.1. NM_001304390.1.
NP_001291320.1. NM_001304391.1.
NP_001291344.1. NM_001304415.1.
NP_001291345.1. NM_001304416.1.
NP_001291346.1. NM_001304417.1.
NP_056239.3. NM_015424.5. [Q6WN34-2]
UniGeneiHs.432379.

3D structure databases

ProteinModelPortaliQ6WN34.
SMRiQ6WN34.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117395. 1 interactor.

PTM databases

iPTMnetiQ6WN34.
PhosphoSitePlusiQ6WN34.

Polymorphism and mutation databases

DMDMi62900089.

Proteomic databases

PeptideAtlasiQ6WN34.
PRIDEiQ6WN34.

Protocols and materials databases

DNASUi25884.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000263671; ENSP00000263671; ENSG00000054938. [Q6WN34-2]
ENST00000376332; ENSP00000365510; ENSG00000054938. [Q6WN34-1]
ENST00000528471; ENSP00000434589; ENSG00000054938. [Q6WN34-5]
ENST00000534276; ENSP00000432055; ENSG00000054938. [Q6WN34-4]
GeneIDi25884.
KEGGihsa:25884.
UCSCiuc001ovh.5. human. [Q6WN34-1]

Organism-specific databases

CTDi25884.
GeneCardsiCHRDL2.
HGNCiHGNC:24168. CHRDL2.
HPAiHPA021771.
MIMi613127. gene.
neXtProtiNX_Q6WN34.
OpenTargetsiENSG00000054938.
PharmGKBiPA134883081.
GenAtlasiSearch...

Phylogenomic databases

GeneTreeiENSGT00730000110792.
HOGENOMiHOG000049211.
HOVERGENiHBG051113.
InParanoidiQ6WN34.
KOiK17280.
OMAiVKGIFHL.
OrthoDBiEOG091G0WK8.
PhylomeDBiQ6WN34.
TreeFamiTF106451.

Miscellaneous databases

GenomeRNAii25884.
PROiQ6WN34.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000054938.
CleanExiHS_CHRDL2.
ExpressionAtlasiQ6WN34. baseline and differential.
GenevisibleiQ6WN34. HS.

Family and domain databases

InterProiIPR001007. VWF_dom.
[Graphical view]
PfamiPF00093. VWC. 3 hits.
[Graphical view]
SMARTiSM00214. VWC. 3 hits.
[Graphical view]
PROSITEiPS01208. VWFC_1. 3 hits.
PS50184. VWFC_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCRDL2_HUMAN
AccessioniPrimary (citable) accession number: Q6WN34
Secondary accession number(s): A5PKU9
, Q6WN30, Q6WN31, Q6WN32, Q7Z5J3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

According to PubMed:14660436, interacts with BMP2, BMP4, BMP5, BMP6, BMP7 but not INHBA. According to PubMed:15094188, interacts with INHBA but not BMP2, BMP4 and BMP6.Curated

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.