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Protein

Nostrin

Gene

Nostrin

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Multivalent adapter protein which may decrease NOS3 activity by inducing its translocation away from the plasma membrane.By similarity1 Publication

GO - Molecular functioni

  • DNA binding Source: MGI

GO - Biological processi

  • endocytosis Source: UniProtKB-KW
  • negative regulation of transcription, DNA-templated Source: MGI
Complete GO annotation...

Keywords - Biological processi

Endocytosis

Enzyme and pathway databases

ReactomeiR-MMU-203641. NOSTRIN mediated eNOS trafficking.

Names & Taxonomyi

Protein namesi
Recommended name:
Nostrin
Alternative name(s):
Disabled homolog 2-interacting protein 2
Short name:
Dab2-interacting protein 2
Nitric oxide synthase trafficker
eNOS-trafficking inducer
Gene namesi
Name:Nostrin
Synonyms:Daip2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:3606242. Nostrin.

Subcellular locationi

  • Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
  • Cytoplasmic vesicle By similarity
  • Cytoplasmcytoskeleton By similarity
  • Cytoplasm 1 Publication
  • Nucleus 1 Publication

  • Note: Enriched in selected actin structures.By similarity

GO - Cellular componenti

  • cytoplasmic, membrane-bounded vesicle Source: UniProtKB-SubCell
  • cytoskeleton Source: UniProtKB-SubCell
  • nucleus Source: MGI
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 506506NostrinPRO_0000289090Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei114 – 1141PhosphoserineBy similarity
Modified residuei479 – 4791PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ6WKZ7.
MaxQBiQ6WKZ7.
PaxDbiQ6WKZ7.
PRIDEiQ6WKZ7.

PTM databases

iPTMnetiQ6WKZ7.
PhosphoSiteiQ6WKZ7.

Expressioni

Inductioni

In F9 cells, by retinoic acid (at protein level).1 Publication

Gene expression databases

BgeeiQ6WKZ7.
CleanExiMM_NOSTRIN.
GenevisibleiQ6WKZ7. MM.

Interactioni

Subunit structurei

Homotrimer. Interacts with NOS3, DNM2, WASL and CAV1 (By similarity). Interacts with DAB2.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Dab2P980782EBI-1391878,EBI-1391846

Protein-protein interaction databases

IntActiQ6WKZ7. 3 interactions.
MINTiMINT-1212890.
STRINGi10090.ENSMUSP00000036923.

Structurei

3D structure databases

ProteinModelPortaliQ6WKZ7.
SMRiQ6WKZ7. Positions 14-262, 436-506.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 260260F-BARPROSITE-ProRule annotationAdd
BLAST
Domaini438 – 49760SH3PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili160 – 23071Sequence analysisAdd
BLAST
Coiled coili305 – 33430Sequence analysisAdd
BLAST

Domaini

The SH3 domain mediates interaction with NOS3, DNM2 and WASL.By similarity
The F-BAR domain is necessary for membrane targeting.By similarity

Sequence similaritiesi

Contains 1 F-BAR domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiKOG4429. Eukaryota.
ENOG410ZDFS. LUCA.
GeneTreeiENSGT00510000048120.
HOGENOMiHOG000113830.
HOVERGENiHBG101730.
InParanoidiQ6WKZ7.
OMAiQVLGRCK.
OrthoDBiEOG7BCNBJ.
PhylomeDBiQ6WKZ7.
TreeFamiTF318059.

Family and domain databases

InterProiIPR031160. F_BAR.
IPR001060. FCH_dom.
IPR001452. SH3_domain.
IPR013315. Spectrin_alpha_SH3.
[Graphical view]
PfamiPF00611. FCH. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
PR01887. SPECTRNALPHA.
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51741. F_BAR. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6WKZ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRDPLTDCSY NKVYKSLKEF AQHGDNFCKQ ITSVLQQRAN LEISYAKGLQ
60 70 80 90 100
KLAVRLSKAL QSTKKNCLST AWAWASESMK SAADLHQKLG KAIELEAIKP
110 120 130 140 150
THQVLSMQEK KRKSLDNEVE KTANLVINNW NQQIKAKKKL MMSTKKHEAL
160 170 180 190 200
FHLVESSKQS LTQKEKQKLL NKLKKSTEKL EKEDESYYQK NMAGYSTRLK
210 220 230 240 250
WESTLENCYK SMLELEKERI QLLCNNLNQY SQHISLFGQT LTTCHTQIHC
260 270 280 290 300
AISKVDVEKD IQALMEETAI LSIENKSELL LADYFEEDPK NPMDKERRKS
310 320 330 340 350
LLKPKLGRLQ RDIEKASRDK EGLERKLKAL ASSSSFSDAK SQKDMETLMD
360 370 380 390 400
ENSLKLDLLQ ANSYKLSSVL ADLEQRPKPC HPCSTCIFKW KEKEHSHTYV
410 420 430 440 450
KISRPLLTKR LEKAESKAPA GGQNNPSSSP SGSTVSQASK HLCKALYTFQ
460 470 480 490 500
ARQDDELNLE KGDIVTVHEK KEEGWWFGSL KGKRGHFPAA YVEELPPKAG

NTATQA
Length:506
Mass (Da):57,673
Last modified:May 29, 2007 - v2
Checksum:i78ADED09B493BEC4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti50 – 501Q → R in AAP32311 (PubMed:15596140).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY280963 mRNA. Translation: AAP32311.1.
AJ564264 mRNA. Translation: CAD91927.1.
AL929221 Genomic DNA. Translation: CAM24122.1.
BC069942 mRNA. Translation: AAH69942.1.
AK052926 mRNA. Translation: BAC35204.1.
CCDSiCCDS16087.1.
RefSeqiNP_853525.3. NM_181547.3.
UniGeneiMm.90047.

Genome annotation databases

EnsembliENSMUST00000041865; ENSMUSP00000036923; ENSMUSG00000034738.
GeneIDi329416.
KEGGimmu:329416.
UCSCiuc008jxt.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY280963 mRNA. Translation: AAP32311.1.
AJ564264 mRNA. Translation: CAD91927.1.
AL929221 Genomic DNA. Translation: CAM24122.1.
BC069942 mRNA. Translation: AAH69942.1.
AK052926 mRNA. Translation: BAC35204.1.
CCDSiCCDS16087.1.
RefSeqiNP_853525.3. NM_181547.3.
UniGeneiMm.90047.

3D structure databases

ProteinModelPortaliQ6WKZ7.
SMRiQ6WKZ7. Positions 14-262, 436-506.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ6WKZ7. 3 interactions.
MINTiMINT-1212890.
STRINGi10090.ENSMUSP00000036923.

PTM databases

iPTMnetiQ6WKZ7.
PhosphoSiteiQ6WKZ7.

Proteomic databases

EPDiQ6WKZ7.
MaxQBiQ6WKZ7.
PaxDbiQ6WKZ7.
PRIDEiQ6WKZ7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000041865; ENSMUSP00000036923; ENSMUSG00000034738.
GeneIDi329416.
KEGGimmu:329416.
UCSCiuc008jxt.2. mouse.

Organism-specific databases

CTDi115677.
MGIiMGI:3606242. Nostrin.

Phylogenomic databases

eggNOGiKOG4429. Eukaryota.
ENOG410ZDFS. LUCA.
GeneTreeiENSGT00510000048120.
HOGENOMiHOG000113830.
HOVERGENiHBG101730.
InParanoidiQ6WKZ7.
OMAiQVLGRCK.
OrthoDBiEOG7BCNBJ.
PhylomeDBiQ6WKZ7.
TreeFamiTF318059.

Enzyme and pathway databases

ReactomeiR-MMU-203641. NOSTRIN mediated eNOS trafficking.

Miscellaneous databases

NextBioi398743.
PROiQ6WKZ7.
SOURCEiSearch...

Gene expression databases

BgeeiQ6WKZ7.
CleanExiMM_NOSTRIN.
GenevisibleiQ6WKZ7. MM.

Family and domain databases

InterProiIPR031160. F_BAR.
IPR001060. FCH_dom.
IPR001452. SH3_domain.
IPR013315. Spectrin_alpha_SH3.
[Graphical view]
PfamiPF00611. FCH. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
PR01887. SPECTRNALPHA.
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51741. F_BAR. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of mouse disabled 2-interacting protein 2, a mouse orthologue of human NOSTRIN."
    Choi Y.-J., Cho S.-Y., Kim H.-W., Kim J.-A., Bae S.-H., Park S.-S.
    Biochem. Biophys. Res. Commun. 326:594-599(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION BY RETINOIC ACID, INTERACTION WITH DAB2, SUBCELLULAR LOCATION.
    Tissue: Teratocarcinoma.
  2. "Characterization of NOSTRIN - a novel eNOS binding protein."
    Opitz N.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/cJ.
    Tissue: Heart.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo.
  5. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-177.
    Strain: C57BL/6J.
    Tissue: Heart.
  6. "Mouse disabled 2-interacting protein 2 functions as a transcriptional repressor through direct binding onto its own promoter."
    Kim H.-W., Choi Y.-J., Kim J.-A., Bae S.-H., Kim K.-R., Park S.-S.
    Biochem. Biophys. Res. Commun. 337:75-81(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-479, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  8. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-479, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-479, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Heart, Kidney, Liver, Lung and Spleen.

Entry informationi

Entry nameiNOSTN_MOUSE
AccessioniPrimary (citable) accession number: Q6WKZ7
Secondary accession number(s): Q7TSK5, Q8BWC2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: March 16, 2016
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Seems to repress its own transcription.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.