Myosin phosphatase Rho-interacting protein

Names and origin

Protein names

Recommended name:
    Myosin phosphatase Rho-interacting protein
      Short name=M-RIP
Alternative name(s):
    Rho-interacting protein 3
      Short name=RIP3
    p116Rip

Gene names

Name:	MPRIP
Synonyms:	KIAA0864, MRIP, RHOIP3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	1025 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells.
Subunit structure	Binds F-actin through its N-terminus By similarity. Binds RHOA, PPP1R12A/MBS and PPP1R12C/MBS85 through adjacent coiled coil domains.
Subcellular location	Cytoplasm › cytoskeleton. Note= Colocalizes with F-actin.
Sequence similarities	Contains 2 PH domains.

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Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Repeat
Ligand	Actin-binding
PTM	Phosphoprotein
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW cytoskeleton Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	actin binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
YWHAB	P31946	1	EBI-1022605,EBI-359815
YWHAG	P61981	1	EBI-1022605,EBI-359832
YWHAQ	P27348	1	EBI-1022605,EBI-359854

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Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6WCQ1-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6WCQ1-2) The sequence of this isoform differs from the canonical sequence as follows: 1017-1025: VIEQVSWDT → LKEGLTVQERLKLFESRDLKKD

Isoform 3 (identifier: Q6WCQ1-3) The sequence of this isoform differs from the canonical sequence as follows: 346-383: Missing. 1017-1025: VIEQVSWDT → LKEGLTVQERLKLFESRDLKKD

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

2 – 1025

1024

Myosin phosphatase Rho-interacting protein

PRO_0000223930

Regions

Domain

43 – 150

108

PH 1

Domain

387 – 483

97

PH 2

Region

1 – 383

383

Interaction with F-actin By similarity

Region

546 – 824

279

Interaction with RHOA

Region

824 – 879

56

Interaction with PPP1R12A

Coiled coil

673 – 977

305

Potential

Compositional bias

179 – 252

74

Ser-rich

Amino acid modifications

Modified residue

181

1

Phosphoserine By similarity

Modified residue

186

1

Phosphoserine By similarity

Modified residue

220

1

Phosphoserine

Modified residue

224

1

Phosphoserine

Modified residue

226

1

Phosphoserine

Modified residue

289

1

Phosphoserine

Modified residue

292

1

Phosphoserine

Modified residue

294

1

Phosphoserine

Modified residue

295

1

Phosphothreonine

Modified residue

320

1

Phosphothreonine By similarity

Modified residue

362

1

Phosphoserine

Modified residue

365

1

Phosphoserine

Modified residue

619

1

Phosphoserine

Modified residue

891

1

Phosphoserine

Modified residue

977

1

Phosphoserine

Modified residue

993

1

Phosphoserine

Modified residue

1014

1

Phosphoserine By similarity

Modified residue

1016

1

Phosphoserine By similarity

Natural variations

Alternative sequence

346 – 383

38

Missing in isoform 3.

VSP_051947

Alternative sequence

1017 – 1025

9

VIEQVSWDT → LKEGLTVQERLKLFESRDLK KD in isoform 2 and isoform 3.

VSP_051948

Experimental info

Sequence conflict

31

1

S → P in AAQ63176. Ref.1

Sequence conflict

184

1

S → R in BAD89507. Ref.2

Sequence conflict

188 – 189

2

Missing in BAD89507. Ref.2

Sequence conflict

188 – 189

2

Missing in AAH09982. Ref.6

Sequence conflict

189

1

Missing in AAQ63176. Ref.1

Sequence conflict

189

1

Missing in BAC78198. Ref.3

Sequence conflict

189

1

Missing in CAD39169. Ref.5

Sequence conflict

235

1

S → F in BAD89507. Ref.2

Sequence conflict

327

1

P → Q in BAC78198. Ref.3

Sequence conflict

327

1

P → Q in CAD39169. Ref.5

Sequence conflict

352

1

P → S in BAD89507. Ref.2

Sequence conflict

528

1

R → T in AAQ63176. Ref.1

Sequence conflict

550

1

P → L in BAC78198. Ref.3

Sequence conflict

550

1

P → L in CAD39169. Ref.5

Sequence conflict

578

1

P → S in AAQ63176. Ref.1

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Sequences

Sequence		Length	Mass (Da)
Isoform 1 [UniParc]. Last modified November 25, 2008. Version 3. Checksum: 5B7A30CDB3685E4E Show »	FASTA	1,025	116,533
10 20 30 40 50 60 MSAAKENPCR KFQANIFNKS KCQNCFKPRE SHLLNDEDLT QAKPIYGGWL LLAPDGTDFD 70 80 90 100 110 120 NPVHRSRKWQ RRFFILYEHG LLRYALDEMP TTLPQGTINM NQCTDVVDGE GRTGQKFSLC 130 140 150 160 170 180 ILTPEKEHFI RAETKEIVSG WLEMLMVYPR TNKQNQKKKR KVEPPTPQEP GPAKVAVTSS 190 200 210 220 230 240 SSSSSSSSSI PSAEKVPTTK STLWQEEMRT KDQPDGSSLS PAQSPSQSQP PAASSLREPG 250 260 270 280 290 300 LESKEEESAM SSDRMDCGRK VRVESGYFSL EKTKQDLKAE EQQLPPPLSP PSPSTPNHRR 310 320 330 340 350 360 SQVIEKFEAL DIEKAEHMET NAVGPSPSSD TRQGRSEKRA FPRKRDFTNE APPAPLPDAS 370 380 390 400 410 420 ASPLSPHRRA KSLDRRSTEP SVTPDLLNFK KGWLTKQYED GQWKKHWFVL ADQSLRYYRD 430 440 450 460 470 480 SVAEEAADLD GEIDLSACYD VTEYPVQRNY GFQIHTKEGE FTLSAMTSGI RRNWIQTIMK 490 500 510 520 530 540 HVHPTTAPDV TSSLPEEKNK SSCSFETCPR PTEKQEAELG EPDPEQKRSR ARERRREGRS 550 560 570 580 590 600 KTFDWAEFRP IQQALAQERV GGVGPADTHE PLRPEAEPGE LERERARRRE ERRKRFGMLD 610 620 630 640 650 660 ATDGPGTEDA ALRMEVDRSP GLPMSDLKTH NVHVEIEQRW HQVETTPLRE EKQVPIAPVH 670 680 690 700 710 720 LSSEDGGDRL STHELTSLLE KELEQSQKEA SDLLEQNRLL QDQLRVALGR EQSAREGYVL 730 740 750 760 770 780 QATCERGFAA MEETHQKKIE DLQRQHQREL EKLREEKDRL LAEETAATIS AIEAMKNAHR 790 800 810 820 830 840 EEMERELEKS QRSQISSVNS DVEALRRQYL EELQSVQREL EVLSEQYSQK CLENAHLAQA 850 860 870 880 890 900 LEAERQALRQ CQRENQELNA HNQELNNRLA AEITRLRTLL TGDGGGEATG SPLAQGKDAY 910 920 930 940 950 960 ELEVLLRVKE SEIQYLKQEI SSLKDELQTA LRDKKYASDK YKDIYTELSI AKAKADCDIS 970 980 990 1000 1010 1020 RLKEQLKAAT EALGEKSPDS ATVSGYDIMK SKSNPDFLKK DRSCVTRQLR NIRSKSVIEQ VSWDT « Hide
Isoform 2 [UniParc]. Checksum: EE674842C8133516 Show »		1,038	118,103
10 20 30 40 50 60 MSAAKENPCR KFQANIFNKS KCQNCFKPRE SHLLNDEDLT QAKPIYGGWL LLAPDGTDFD 70 80 90 100 110 120 NPVHRSRKWQ RRFFILYEHG LLRYALDEMP TTLPQGTINM NQCTDVVDGE GRTGQKFSLC 130 140 150 160 170 180 ILTPEKEHFI RAETKEIVSG WLEMLMVYPR TNKQNQKKKR KVEPPTPQEP GPAKVAVTSS 190 200 210 220 230 240 SSSSSSSSSI PSAEKVPTTK STLWQEEMRT KDQPDGSSLS PAQSPSQSQP PAASSLREPG 250 260 270 280 290 300 LESKEEESAM SSDRMDCGRK VRVESGYFSL EKTKQDLKAE EQQLPPPLSP PSPSTPNHRR 310 320 330 340 350 360 SQVIEKFEAL DIEKAEHMET NAVGPSPSSD TRQGRSEKRA FPRKRDFTNE APPAPLPDAS 370 380 390 400 410 420 ASPLSPHRRA KSLDRRSTEP SVTPDLLNFK KGWLTKQYED GQWKKHWFVL ADQSLRYYRD 430 440 450 460 470 480 SVAEEAADLD GEIDLSACYD VTEYPVQRNY GFQIHTKEGE FTLSAMTSGI RRNWIQTIMK 490 500 510 520 530 540 HVHPTTAPDV TSSLPEEKNK SSCSFETCPR PTEKQEAELG EPDPEQKRSR ARERRREGRS 550 560 570 580 590 600 KTFDWAEFRP IQQALAQERV GGVGPADTHE PLRPEAEPGE LERERARRRE ERRKRFGMLD 610 620 630 640 650 660 ATDGPGTEDA ALRMEVDRSP GLPMSDLKTH NVHVEIEQRW HQVETTPLRE EKQVPIAPVH 670 680 690 700 710 720 LSSEDGGDRL STHELTSLLE KELEQSQKEA SDLLEQNRLL QDQLRVALGR EQSAREGYVL 730 740 750 760 770 780 QATCERGFAA MEETHQKKIE DLQRQHQREL EKLREEKDRL LAEETAATIS AIEAMKNAHR 790 800 810 820 830 840 EEMERELEKS QRSQISSVNS DVEALRRQYL EELQSVQREL EVLSEQYSQK CLENAHLAQA 850 860 870 880 890 900 LEAERQALRQ CQRENQELNA HNQELNNRLA AEITRLRTLL TGDGGGEATG SPLAQGKDAY 910 920 930 940 950 960 ELEVLLRVKE SEIQYLKQEI SSLKDELQTA LRDKKYASDK YKDIYTELSI AKAKADCDIS 970 980 990 1000 1010 1020 RLKEQLKAAT EALGEKSPDS ATVSGYDIMK SKSNPDFLKK DRSCVTRQLR NIRSKSLKEG 1030 LTVQERLKLF ESRDLKKD « Hide
Isoform 3 [UniParc]. Checksum: FCFDB3210A73EEF9 Show »		1,000	114,049
10 20 30 40 50 60 MSAAKENPCR KFQANIFNKS KCQNCFKPRE SHLLNDEDLT QAKPIYGGWL LLAPDGTDFD 70 80 90 100 110 120 NPVHRSRKWQ RRFFILYEHG LLRYALDEMP TTLPQGTINM NQCTDVVDGE GRTGQKFSLC 130 140 150 160 170 180 ILTPEKEHFI RAETKEIVSG WLEMLMVYPR TNKQNQKKKR KVEPPTPQEP GPAKVAVTSS 190 200 210 220 230 240 SSSSSSSSSI PSAEKVPTTK STLWQEEMRT KDQPDGSSLS PAQSPSQSQP PAASSLREPG 250 260 270 280 290 300 LESKEEESAM SSDRMDCGRK VRVESGYFSL EKTKQDLKAE EQQLPPPLSP PSPSTPNHRR 310 320 330 340 350 360 SQVIEKFEAL DIEKAEHMET NAVGPSPSSD TRQGRSEKRA FPRKRPDLLN FKKGWLTKQY 370 380 390 400 410 420 EDGQWKKHWF VLADQSLRYY RDSVAEEAAD LDGEIDLSAC YDVTEYPVQR NYGFQIHTKE 430 440 450 460 470 480 GEFTLSAMTS GIRRNWIQTI MKHVHPTTAP DVTSSLPEEK NKSSCSFETC PRPTEKQEAE 490 500 510 520 530 540 LGEPDPEQKR SRARERRREG RSKTFDWAEF RPIQQALAQE RVGGVGPADT HEPLRPEAEP 550 560 570 580 590 600 GELERERARR REERRKRFGM LDATDGPGTE DAALRMEVDR SPGLPMSDLK THNVHVEIEQ 610 620 630 640 650 660 RWHQVETTPL REEKQVPIAP VHLSSEDGGD RLSTHELTSL LEKELEQSQK EASDLLEQNR 670 680 690 700 710 720 LLQDQLRVAL GREQSAREGY VLQATCERGF AAMEETHQKK IEDLQRQHQR ELEKLREEKD 730 740 750 760 770 780 RLLAEETAAT ISAIEAMKNA HREEMERELE KSQRSQISSV NSDVEALRRQ YLEELQSVQR 790 800 810 820 830 840 ELEVLSEQYS QKCLENAHLA QALEAERQAL RQCQRENQEL NAHNQELNNR LAAEITRLRT 850 860 870 880 890 900 LLTGDGGGEA TGSPLAQGKD AYELEVLLRV KESEIQYLKQ EISSLKDELQ TALRDKKYAS 910 920 930 940 950 960 DKYKDIYTEL SIAKAKADCD ISRLKEQLKA ATEALGEKSP DSATVSGYDI MKSKSNPDFL 970 980 990 1000 KKDRSCVTRQ LRNIRSKSLK EGLTVQERLK LFESRDLKKD « Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Myosin phosphatase-Rho interacting protein. A new member of the myosin phosphatase complex that directly binds RhoA." Surks H.K., Richards C.T., Mendelsohn M.E. J. Biol. Chem. 278:51484-51493(2003) [PubMed: 14506264] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, INTERACTION WITH PPP1R12A AND RHOA. Tissue: Aorta.
[2]	"p116Rip decreases myosin II phosphorylation by activating myosin light chain phosphatase and by inactivating RhoA." Koga Y., Ikebe M. J. Biol. Chem. 280:4983-4991(2005) [PubMed: 15545284] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, INTERACTION WITH PPP1R12A.
[3]	"Cloning of human orthologue RHOIP3 of Mus Rhoip3." Inazawa J., Imoto I. Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[4]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. Nusbaum C. Nature 440:1045-1049(2006) [PubMed: 16625196] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	The German cDNA consortium Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 38-1025 (ISOFORM 1). Tissue: Melanoma.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 157-1025 (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 194-1025 (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 377-1025 (ISOFORM 2). Tissue: Brain, Lymph and Muscle.
[7]	"M-RIP targets myosin phosphatase to stress fibers to regulate myosin light chain phosphorylation in vascular smooth muscle cells." Surks H.K., Riddick N., Ohtani K. J. Biol. Chem. 280:42543-42551(2005) [PubMed: 16257966] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH PPP1R12A AND F-ACTIN.
[8]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-220; SER-224; SER-365; SER-619 AND SER-993, MASS SPECTROMETRY. Tissue: Epithelium.
[9]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-365 AND SER-993, MASS SPECTROMETRY.
[10]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-220; SER-224; SER-226; SER-289; SER-292; SER-294; THR-295; SER-362; SER-365; SER-619; SER-891 AND SER-977, MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	AY296247 mRNA. Translation: AAQ63176.1. AB189741 mRNA. Translation: BAD89507.1. AB098507 mRNA. Translation: BAC78198.1. AC079111 Genomic DNA. No translation available. AC055811 Genomic DNA. No translation available. AL834513 mRNA. Translation: CAD39169.1. BC009982 mRNA. Translation: AAH09982.2. BC075847 mRNA. Translation: AAH75847.1. BC105987 mRNA. Translation: AAI05988.1.
RefSeq	NP_055949.2. NP_958431.2.
UniGene	Hs.462341
3D structure databases
HSSP	HSSP built from PDB template 1FAO based on UniProtKB Q9UN19.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q6WCQ1.
PTM databases
PhosphoSite	Q6WCQ1.
Genome annotation databases
Ensembl	ENSG00000133030. Homo sapiens. [Contig view]
GeneID	23164.
KEGG	hsa:23164.
Organism-specific databases
H-InvDB	HIX0013577.
HGNC	HGNC:30321. MPRIP.
HUGE	Search...
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOVERGEN	Q6WCQ1.
Gene expression databases
ArrayExpress	Q6WCQ1.
GermOnline	ENSG00000133030. Homo sapiens.
Family and domain databases
InterPro	IPR001849. PH. IPR011993. PH_type. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 2 hits.
Pfam	PF00169. PH. 2 hits. [Graphical view]
SMART	SM00233. PH. 2 hits. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	44551.

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Entry information

Entry name	MPRIP_HUMAN
Accession	Primary (citable) accessio

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Keywords

Gene Ontology (GO)

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information