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Protein

Myosin phosphatase Rho-interacting protein

Gene

MPRIP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells.By similarity2 Publications

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL
Complete GO annotation...

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

ReactomeiR-HSA-6802952. Signaling by BRAF and RAF fusions.
SIGNORiQ6WCQ1.

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin phosphatase Rho-interacting protein
Short name:
M-RIP
Alternative name(s):
Rho-interacting protein 3
Short name:
RIP3
p116Rip
Gene namesi
Name:MPRIP
Synonyms:KIAA0864, MRIP, RHOIP3Imported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:30321. MPRIP.

Subcellular locationi

GO - Cellular componenti

  • actin cytoskeleton Source: HPA
  • cell-cell adherens junction Source: BHF-UCL
  • cytoplasm Source: HPA
  • focal adhesion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Organism-specific databases

DisGeNETi23164.
OpenTargetsiENSG00000133030.
PharmGKBiPA162396092.

Polymorphism and mutation databases

BioMutaiMPRIP.
DMDMi215274136.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002239302 – 1025Myosin phosphatase Rho-interacting proteinAdd BLAST1024

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei192PhosphoserineCombined sources1
Modified residuei217PhosphoserineCombined sources1
Modified residuei218PhosphoserineBy similarity1
Modified residuei220PhosphoserineCombined sources1
Modified residuei224PhosphoserineCombined sources1
Modified residuei226PhosphoserineCombined sources1
Modified residuei265PhosphoserineCombined sources1
Modified residuei269PhosphoserineCombined sources1
Modified residuei289PhosphoserineCombined sources1
Modified residuei292PhosphoserineCombined sources1
Modified residuei295PhosphothreonineCombined sources1
Modified residuei326PhosphoserineCombined sources1
Modified residuei348PhosphothreonineCombined sources1
Modified residuei362PhosphoserineCombined sources1
Modified residuei365PhosphoserineCombined sources1
Modified residuei493PhosphoserineCombined sources1
Modified residuei619PhosphoserineCombined sources1
Modified residuei646PhosphothreonineCombined sources1
Modified residuei663PhosphoserineCombined sources1
Modified residuei800PhosphoserineCombined sources1
Modified residuei891PhosphoserineCombined sources1
Modified residuei977PhosphoserineCombined sources1
Modified residuei993PhosphoserineCombined sources1
Modified residuei1014PhosphoserineBy similarity1
Modified residuei1016PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ6WCQ1.
MaxQBiQ6WCQ1.
PaxDbiQ6WCQ1.
PeptideAtlasiQ6WCQ1.
PRIDEiQ6WCQ1.

PTM databases

iPTMnetiQ6WCQ1.
PhosphoSitePlusiQ6WCQ1.
SwissPalmiQ6WCQ1.

Expressioni

Gene expression databases

BgeeiENSG00000133030.
CleanExiHS_MPRIP.
ExpressionAtlasiQ6WCQ1. baseline and differential.
GenevisibleiQ6WCQ1. HS.

Organism-specific databases

HPAiHPA022901.

Interactioni

Subunit structurei

Binds F-actin through its N-terminus. Interacts with MYZAP (By similarity). Binds RHOA, PPP1R12A/MBS and PPP1R12C/MBS85 through adjacent coiled coil domains.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Ppp1r12aQ107286EBI-1022605,EBI-918263From a different organism.

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL

Protein-protein interaction databases

BioGridi116776. 73 interactors.
IntActiQ6WCQ1. 44 interactors.
MINTiMINT-1161017.
STRINGi9606.ENSP00000379156.

Structurei

3D structure databases

ProteinModelPortaliQ6WCQ1.
SMRiQ6WCQ1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini43 – 150PH 1PROSITE-ProRule annotationAdd BLAST108
Domaini387 – 483PH 2PROSITE-ProRule annotationAdd BLAST97

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 383Interaction with F-actinBy similarityAdd BLAST383
Regioni546 – 824Interaction with RHOA1 PublicationAdd BLAST279
Regioni824 – 879Interaction with PPP1R12AAdd BLAST56

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili673 – 977Sequence analysisAdd BLAST305

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi179 – 252Ser-richAdd BLAST74

Sequence similaritiesi

Contains 2 PH domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG4807. Eukaryota.
ENOG410ZA8F. LUCA.
GeneTreeiENSGT00530000063678.
HOVERGENiHBG023864.
InParanoidiQ6WCQ1.
PhylomeDBiQ6WCQ1.
TreeFamiTF329258.

Family and domain databases

Gene3Di2.30.29.30. 2 hits.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF00169. PH. 2 hits.
[Graphical view]
SMARTiSM00233. PH. 2 hits.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 2 hits.
PROSITEiPS50003. PH_DOMAIN. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q6WCQ1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSAAKENPCR KFQANIFNKS KCQNCFKPRE SHLLNDEDLT QAKPIYGGWL
60 70 80 90 100
LLAPDGTDFD NPVHRSRKWQ RRFFILYEHG LLRYALDEMP TTLPQGTINM
110 120 130 140 150
NQCTDVVDGE GRTGQKFSLC ILTPEKEHFI RAETKEIVSG WLEMLMVYPR
160 170 180 190 200
TNKQNQKKKR KVEPPTPQEP GPAKVAVTSS SSSSSSSSSI PSAEKVPTTK
210 220 230 240 250
STLWQEEMRT KDQPDGSSLS PAQSPSQSQP PAASSLREPG LESKEEESAM
260 270 280 290 300
SSDRMDCGRK VRVESGYFSL EKTKQDLKAE EQQLPPPLSP PSPSTPNHRR
310 320 330 340 350
SQVIEKFEAL DIEKAEHMET NAVGPSPSSD TRQGRSEKRA FPRKRDFTNE
360 370 380 390 400
APPAPLPDAS ASPLSPHRRA KSLDRRSTEP SVTPDLLNFK KGWLTKQYED
410 420 430 440 450
GQWKKHWFVL ADQSLRYYRD SVAEEAADLD GEIDLSACYD VTEYPVQRNY
460 470 480 490 500
GFQIHTKEGE FTLSAMTSGI RRNWIQTIMK HVHPTTAPDV TSSLPEEKNK
510 520 530 540 550
SSCSFETCPR PTEKQEAELG EPDPEQKRSR ARERRREGRS KTFDWAEFRP
560 570 580 590 600
IQQALAQERV GGVGPADTHE PLRPEAEPGE LERERARRRE ERRKRFGMLD
610 620 630 640 650
ATDGPGTEDA ALRMEVDRSP GLPMSDLKTH NVHVEIEQRW HQVETTPLRE
660 670 680 690 700
EKQVPIAPVH LSSEDGGDRL STHELTSLLE KELEQSQKEA SDLLEQNRLL
710 720 730 740 750
QDQLRVALGR EQSAREGYVL QATCERGFAA MEETHQKKIE DLQRQHQREL
760 770 780 790 800
EKLREEKDRL LAEETAATIS AIEAMKNAHR EEMERELEKS QRSQISSVNS
810 820 830 840 850
DVEALRRQYL EELQSVQREL EVLSEQYSQK CLENAHLAQA LEAERQALRQ
860 870 880 890 900
CQRENQELNA HNQELNNRLA AEITRLRTLL TGDGGGEATG SPLAQGKDAY
910 920 930 940 950
ELEVLLRVKE SEIQYLKQEI SSLKDELQTA LRDKKYASDK YKDIYTELSI
960 970 980 990 1000
AKAKADCDIS RLKEQLKAAT EALGEKSPDS ATVSGYDIMK SKSNPDFLKK
1010 1020
DRSCVTRQLR NIRSKSVIEQ VSWDT
Length:1,025
Mass (Da):116,533
Last modified:November 25, 2008 - v3
Checksum:i5B7A30CDB3685E4E
GO
Isoform 21 Publication (identifier: Q6WCQ1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1017-1025: VIEQVSWDT → LKEGLTVQERLKLFESRDLKKD

Show »
Length:1,038
Mass (Da):118,103
Checksum:iEE674842C8133516
GO
Isoform 3Curated (identifier: Q6WCQ1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     346-383: Missing.
     1017-1025: VIEQVSWDT → LKEGLTVQERLKLFESRDLKKD

Show »
Length:1,000
Mass (Da):114,049
Checksum:iFCFDB3210A73EEF9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti31S → P in AAQ63176 (PubMed:14506264).Curated1
Sequence conflicti184S → R in BAD89507 (PubMed:15545284).Curated1
Sequence conflicti188 – 189Missing in BAD89507 (PubMed:15545284).Curated2
Sequence conflicti188 – 189Missing in AAH09982 (PubMed:15489334).Curated2
Sequence conflicti189Missing in AAQ63176 (PubMed:14506264).Curated1
Sequence conflicti189Missing in BAC78198 (Ref. 3) Curated1
Sequence conflicti189Missing in CAD39169 (PubMed:17974005).Curated1
Sequence conflicti235S → F in BAD89507 (PubMed:15545284).Curated1
Sequence conflicti352P → S in BAD89507 (PubMed:15545284).Curated1
Sequence conflicti528R → T in AAQ63176 (PubMed:14506264).Curated1
Sequence conflicti550P → L in BAC78198 (Ref. 3) Curated1
Sequence conflicti550P → L in CAD39169 (PubMed:17974005).Curated1
Sequence conflicti578P → S in AAQ63176 (PubMed:14506264).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_051203327P → Q.2 PublicationsCorresponds to variant rs3744137dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_051947346 – 383Missing in isoform 3. 1 PublicationAdd BLAST38
Alternative sequenceiVSP_0519481017 – 1025VIEQVSWDT → LKEGLTVQERLKLFESRDLK KD in isoform 2 and isoform 3. 3 Publications9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY296247 mRNA. Translation: AAQ63176.1.
AB189741 mRNA. Translation: BAD89507.1.
AB098507 mRNA. Translation: BAC78198.1.
AC079111 Genomic DNA. No translation available.
AC055811 Genomic DNA. No translation available.
AL834513 mRNA. Translation: CAD39169.1.
BC009982 mRNA. Translation: AAH09982.2.
BC075847 mRNA. Translation: AAH75847.1.
BC105987 mRNA. Translation: AAI05988.1.
CCDSiCCDS32578.1. [Q6WCQ1-1]
CCDS42268.1. [Q6WCQ1-2]
RefSeqiNP_055949.2. NM_015134.3. [Q6WCQ1-2]
NP_958431.2. NM_201274.3. [Q6WCQ1-1]
UniGeneiHs.462341.

Genome annotation databases

EnsembliENST00000341712; ENSP00000342379; ENSG00000133030. [Q6WCQ1-1]
ENST00000395811; ENSP00000379156; ENSG00000133030. [Q6WCQ1-2]
GeneIDi23164.
KEGGihsa:23164.
UCSCiuc002gqu.3. human. [Q6WCQ1-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY296247 mRNA. Translation: AAQ63176.1.
AB189741 mRNA. Translation: BAD89507.1.
AB098507 mRNA. Translation: BAC78198.1.
AC079111 Genomic DNA. No translation available.
AC055811 Genomic DNA. No translation available.
AL834513 mRNA. Translation: CAD39169.1.
BC009982 mRNA. Translation: AAH09982.2.
BC075847 mRNA. Translation: AAH75847.1.
BC105987 mRNA. Translation: AAI05988.1.
CCDSiCCDS32578.1. [Q6WCQ1-1]
CCDS42268.1. [Q6WCQ1-2]
RefSeqiNP_055949.2. NM_015134.3. [Q6WCQ1-2]
NP_958431.2. NM_201274.3. [Q6WCQ1-1]
UniGeneiHs.462341.

3D structure databases

ProteinModelPortaliQ6WCQ1.
SMRiQ6WCQ1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116776. 73 interactors.
IntActiQ6WCQ1. 44 interactors.
MINTiMINT-1161017.
STRINGi9606.ENSP00000379156.

PTM databases

iPTMnetiQ6WCQ1.
PhosphoSitePlusiQ6WCQ1.
SwissPalmiQ6WCQ1.

Polymorphism and mutation databases

BioMutaiMPRIP.
DMDMi215274136.

Proteomic databases

EPDiQ6WCQ1.
MaxQBiQ6WCQ1.
PaxDbiQ6WCQ1.
PeptideAtlasiQ6WCQ1.
PRIDEiQ6WCQ1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000341712; ENSP00000342379; ENSG00000133030. [Q6WCQ1-1]
ENST00000395811; ENSP00000379156; ENSG00000133030. [Q6WCQ1-2]
GeneIDi23164.
KEGGihsa:23164.
UCSCiuc002gqu.3. human. [Q6WCQ1-1]

Organism-specific databases

CTDi23164.
DisGeNETi23164.
GeneCardsiMPRIP.
HGNCiHGNC:30321. MPRIP.
HPAiHPA022901.
MIMi612935. gene.
neXtProtiNX_Q6WCQ1.
OpenTargetsiENSG00000133030.
PharmGKBiPA162396092.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4807. Eukaryota.
ENOG410ZA8F. LUCA.
GeneTreeiENSGT00530000063678.
HOVERGENiHBG023864.
InParanoidiQ6WCQ1.
PhylomeDBiQ6WCQ1.
TreeFamiTF329258.

Enzyme and pathway databases

ReactomeiR-HSA-6802952. Signaling by BRAF and RAF fusions.
SIGNORiQ6WCQ1.

Miscellaneous databases

ChiTaRSiMPRIP. human.
GeneWikiiM-RIP.
GenomeRNAii23164.
PROiQ6WCQ1.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000133030.
CleanExiHS_MPRIP.
ExpressionAtlasiQ6WCQ1. baseline and differential.
GenevisibleiQ6WCQ1. HS.

Family and domain databases

Gene3Di2.30.29.30. 2 hits.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF00169. PH. 2 hits.
[Graphical view]
SMARTiSM00233. PH. 2 hits.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 2 hits.
PROSITEiPS50003. PH_DOMAIN. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMPRIP_HUMAN
AccessioniPrimary (citable) accession number: Q6WCQ1
Secondary accession number(s): Q3KQZ5
, Q5FB94, Q6DHW2, Q7Z5Y2, Q8N390, Q96G40
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: November 25, 2008
Last modified: November 30, 2016
This is version 117 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.