Reviewed, UniProtKB/Swiss-Prot Q6WB83 (LLDD_ALCFA)

Names and origin

Protein names

Recommended name:
    L-lactate dehydrogenase [cytochrome]
    EC=1.1.2.3

Gene names

Name:

lldD

Organism

Alcaligenes faecalis

Taxonomic identifier

511 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Alcaligenes

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-lactate + 2 ferricytochrome c = pyruvate + 2 ferrocytochrome c + 2 H(+).
Cofactor	FMN By similarity.
Sequence similarities	Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family. Contains 1 FMN hydroxy acid dehydrogenase domain.

Ontologies

Keywords
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro L-lactate dehydrogenase (cytochrome) activity Inferred from electronic annotation. Source: HAMAP electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 379	379	L-lactate dehydrogenase [cytochrome]		PRO_0000206330
Regions
	Domain	1 – 379	379	FMN hydroxy acid dehydrogenase
	Nucleotide binding	306 – 330	25	FMN By similarity
Sites
	Active site	275	1	Proton acceptor By similarity
	Binding site	24	1	Substrate Potential
	Binding site	106	1	FMN By similarity
	Binding site	127	1	FMN By similarity
	Binding site	129	1	Substrate By similarity
	Binding site	155	1	FMN By similarity
	Binding site	164	1	Substrate By similarity
	Binding site	251	1	FMN By similarity
	Binding site	278	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6WB83-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 9975D092C4DDF2F9	FASTA	379	41,414
	10 20 30 40 50 60 MIISSSTDYR RAAQKRLPPF LFHYIDGGAY AEHTLRRNVD DLAEVALRQR VLKDMSQLDT 70 80 90 100 110 120 SIDLFGEKLS MPVALSPVGL TGMYARRGEV QAARAADARG IPFTMSSVSV CPIEEVAPRL 130 140 150 160 170 180 SRPMWFQLYV LKDRGFMRNA LERAQAAGCS TLVFTVDMPV PGARYRDAHS GMSGPNAALR 190 200 210 220 230 240 RYAQAVMHPR WAWDVGLLGR PHDLGNISRY LGKPTGLEDY MGYLGANFDP SISWKDLEWI 250 260 270 280 290 300 REFWKGPMLI KGILDPDDAR DAVRFGADGI IVSNHGGRQL DGVLSSARAL PAIADAVKGQ 310 320 330 340 350 360 IKILADSGIR SGLDVVRMIA LGADAAMLGR AYIYALAAAG QSGVDHLLGL IEKEIRVAMT 370 LTSVSSISQI TSELLVREP

References

[1]	"Genes for arsenite oxidation from Alcaligenes faecalis." Silver S., Phung L.T., Malo B.J. Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CCUG 2071 / LMG 3368 / NCIB 8687.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY297781 Genomic DNA. Translation: AAQ19817.1.
3D structure databases
HSSP	HSSP built from PDB template 1AL8 based on UniProtKB P05414.
ModBase	Search...
Family and domain databases
HAMAP	MF_01559. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR012133. Alpha_OHA_DHase_FMN. IPR008259. FMN_hydac_DHase_AS. IPR000262. FMN_OHA_DHase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01070. FMN_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000138. Al-hdrx_acd_dh. 1 hit.
PROSITE	PS00557. FMN_HYDROXY_ACID_DH_1. 1 hit. PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LLDD_ALCFA

Accession

Primary (citable) accession number: Q6WB83

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	July 5, 2004
Last modified:	November 25, 2008
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)