Reviewed, UniProtKB/Swiss-Prot Q6W8P9 (AK1CO_HORSE)

Names and origin

Protein names

Recommended name:
    Aldo-keto reductase family 1 member C23-like protein
    EC=1.1.1.-
Alternative name(s):
    Putative prostaglandin F synthase

Gene names

Name:

PGFS

Organism

Equus caballus (Horse)

Taxonomic identifier

9796 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Perissodactyla › Equidae › Equus

Protein attributes

Sequence length	323 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	NADP-dependent oxidoreductase involved in steroid metabolism. May act on various hydroxysteroids.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Detected in endometrium surface epithelium (at protein level). Detected in endometrium. Ref.1
Sequence similarities	Belongs to the aldo/keto reductase family.

Ontologies

Keywords
Biological process	Lipid metabolism Steroid metabolism
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW steroid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 323	323	Aldo-keto reductase family 1 member C23-like protein		PRO_0000326225
Regions
	Nucleotide binding	13 – 24	12	NADP By similarity
	Nucleotide binding	166 – 167	2	NADP By similarity
	Nucleotide binding	216 – 225	10	NADP By similarity
	Nucleotide binding	270 – 279	10	NADP By similarity
Sites
	Active site	55	1	Proton donor By similarity
	Binding site	31	1	Substrate By similarity
	Binding site	50	1	NADP By similarity
	Binding site	117	1	Substrate By similarity
	Site	84	1	Lowers pKa of active site Tyr By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6W8P9-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 9FFCF6C13D35DB3B	FASTA	323	37,040
	10 20 30 40 50 60 MDPKGWCEKL NDGHVIPVLG FGTYAPEEVP KSRTVEVTKL AIDAGFRHID SAYSYNNEKE 70 80 90 100 110 120 VGQAIRSKIE DGTVKREDIF YTSKLWLTFL RPELVRPALE KSLTNLQLDY VDLYIIHFPI 130 140 150 160 170 180 ALKPGEELFP EDEHGKLIFD TVDLCATWEA MEKCKDAGLA KSIGVSNFNR RQLEMILNKP 190 200 210 220 230 240 GLKYKPVCNQ VECHPYLNQS KLLDFCKSKD IVLVAYGALG TQRLKRWLAP NSPVLLEDPV 250 260 270 280 290 300 LCAMAKKYKR TPALIALRYL LQRGVVVLAK SYNEKRIKEN MQVFEFQLTS EDMKDLDGLN 310 320 RNHRFLPLQI AVDHPEYPFA DEY

References

[1]

"Expression of key prostaglandin synthases in equine endometrium during late diestrus and early pregnancy." Boerboom D., Brown K.A., Vaillancourt D., Poitras P., Goff A.K., Watanabe K., Dore M., Sirois J. Biol. Reprod. 70:391-399(2004) [PubMed: 14561653] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Ovary.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY304536 mRNA. Translation: AAP69945.1.
RefSeq	NP_001075364.1.
UniGene	Eca.12759
3D structure databases
HSSP	HSSP built from PDB template 1AFS based on UniProtKB P23457.
SMR	Q6W8P9. Positions 2-323.
ModBase	Search...
Genome annotation databases
Ensembl	ENSECAG00000018315. Equus caballus. [Contig view]
GeneID	100034026.
KEGG	ecb:100034026.
Phylogenomic databases
HOVERGEN	Q6W8P9.
Family and domain databases
InterPro	IPR001395. Aldo/ket_red. IPR018170. Aldo/ket_reductase_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.100. Aldo/ket_red. 1 hit.
PANTHER	PTHR11732. Aldo/ket_red. 1 hit.
Pfam	PF00248. Aldo_ket_red. 1 hit. [Graphical view]
ProDom	PD000288. Aldo/ket_red. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00798. ALDOKETO_REDUCTASE_1. 1 hit. PS00062. ALDOKETO_REDUCTASE_2. 1 hit. PS00063. ALDOKETO_REDUCTASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AK1CO_HORSE

Accession

Primary (citable) accession number: Q6W8P9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	July 5, 2004
Last modified:	June 16, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)