Q6W3F0 (P4HA3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Prolyl 4-hydroxylase subunit alpha-3
Short name=4-PH alpha-3
EC=1.14.11.2

Alternative name(s):
Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-3

Gene names

Name:

P4ha3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	542 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins By similarity.
Catalytic activity	L-proline-[procollagen] + 2-oxoglutarate + O2 = trans-4-hydroxy-L-proline-[procollagen] + succinate + CO2.
Cofactor	Binds 1 Fe2+ ion per subunit By similarity. Ascorbate By similarity.
Subunit structure	Heterotetramer of two alpha-3 chains and two beta chains (the beta chain is the multi-functional PDI) By similarity.
Subcellular location	Endoplasmic reticulum lumen By similarity.
Post-translational modification	N-glycosylation plays no role in the catalytic activity By similarity.
Sequence similarities	Belongs to the P4HA family. Contains 1 Fe2OG dioxygenase domain. Contains 1 TPR repeat.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Signal TPR repeat
Ligand	Iron Metal-binding Vitamin C
Molecular function	Dioxygenase Oxidoreductase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-ascorbic acid binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW procollagen-proline 4-dioxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6W3F0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6W3F0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-138: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q6W3F0-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-138: Missing.
     444-464: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 542	518	Prolyl 4-hydroxylase subunit alpha-3		PRO_0000317767
Regions
	Repeat	225 – 258	34	TPR
	Domain	420 – 527	108	Fe2OG dioxygenase
	Coiled coil	105 – 129	25	Potential
Sites
	Metal binding	438	1	Iron By similarity
	Metal binding	440	1	Iron By similarity
	Metal binding	508	1	Iron By similarity
	Binding site	518	1	2-oxoglutarate Potential
Amino acid modifications
	Glycosylation	240	1	N-linked (GlcNAc...) Potential
	Glycosylation	480	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 138	138	Missing in isoform 2 and isoform 3.		VSP_031148
	Alternative sequence	444 – 464	21	Missing in isoform 3.		VSP_031149

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 76329127F1C7BA83	FASTA	542	60,975
	10 20 30 40 50 60 MGPGARLALL ALLALGGDPA AATGREDTFS ALTSVARALA PERRLLGTLR RYLRGEEARL 70 80 90 100 110 120 RDLTRFYDKV LSLHEDLKIP VVNPLLAFTV IKRLQSDWRN VVHSLEATEN IRALKDGYEK 130 140 150 160 170 180 VEQDLPAFED LEGAARALMR LQDVYMLNVK GLARGVFQRV TGSSITDLYS PRQLFSLTAD 190 200 210 220 230 240 DCFQVGKVAY DTGDYYHAIP WLEEAVSLFR RAHGEWKTED EASLEDALDY LAFACFQVGN 250 260 270 280 290 300 VSCALSLSRE FLVYSPDNKR MARNVLKYER LLAENGHQMA AETAIQRPNV PHLQTRDTYE 310 320 330 340 350 360 GLCQTLGSQP THYQIPSLYC SYETNSSPYL LLQPARKEVV HLRPLIALYH DFVSDEEAQK 370 380 390 400 410 420 IRELAEPWLQ RSVVASGEKQ LQVEYRISKS AWLKDTVDPM LVTLDHRIAA LTGLDIQPPY 430 440 450 460 470 480 AEYLQVVNYG IGGHYEPHFD HATSPSSPLY RMKSGNRVAT FMIYLSSVEA GGATAFIYGN 490 500 510 520 530 540 FSVPVVKNAA LFWWNLHRSG EGDGDTLHAG CPVLVGDKWV ANKWIHEYGQ EFRRPCSTNP ED
	Isoform 2 [UniParc]. Checksum: 077E5B02617A076E	FASTA	404	45,716
	10 20 30 40 50 60 MRLQDVYMLN VKGLARGVFQ RVTGSSITDL YSPRQLFSLT ADDCFQVGKV AYDTGDYYHA 70 80 90 100 110 120 IPWLEEAVSL FRRAHGEWKT EDEASLEDAL DYLAFACFQV GNVSCALSLS REFLVYSPDN 130 140 150 160 170 180 KRMARNVLKY ERLLAENGHQ MAAETAIQRP NVPHLQTRDT YEGLCQTLGS QPTHYQIPSL 190 200 210 220 230 240 YCSYETNSSP YLLLQPARKE VVHLRPLIAL YHDFVSDEEA QKIRELAEPW LQRSVVASGE 250 260 270 280 290 300 KQLQVEYRIS KSAWLKDTVD PMLVTLDHRI AALTGLDIQP PYAEYLQVVN YGIGGHYEPH 310 320 330 340 350 360 FDHATSPSSP LYRMKSGNRV ATFMIYLSSV EAGGATAFIY GNFSVPVVKN AALFWWNLHR 370 380 390 400 SGEGDGDTLH AGCPVLVGDK WVANKWIHEY GQEFRRPCST NPED
	Isoform 3 [UniParc]. Checksum: 5C6BA82E06D797AC	FASTA	383	43,328
	10 20 30 40 50 60 MRLQDVYMLN VKGLARGVFQ RVTGSSITDL YSPRQLFSLT ADDCFQVGKV AYDTGDYYHA 70 80 90 100 110 120 IPWLEEAVSL FRRAHGEWKT EDEASLEDAL DYLAFACFQV GNVSCALSLS REFLVYSPDN 130 140 150 160 170 180 KRMARNVLKY ERLLAENGHQ MAAETAIQRP NVPHLQTRDT YEGLCQTLGS QPTHYQIPSL 190 200 210 220 230 240 YCSYETNSSP YLLLQPARKE VVHLRPLIAL YHDFVSDEEA QKIRELAEPW LQRSVVASGE 250 260 270 280 290 300 KQLQVEYRIS KSAWLKDTVD PMLVTLDHRI AALTGLDIQP PYAEYLQVVN YGIGGHYEPH 310 320 330 340 350 360 FDHATLSSVE AGGATAFIYG NFSVPVVKNA ALFWWNLHRS GEGDGDTLHA GCPVLVGDKW 370 380 VANKWIHEYG QEFRRPCSTN PED

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of a third human, rat, and mouse collagen prolyl 4-hydroxylase isoenzyme." Kukkola L., Hieta R., Kivirikko K.I., Myllyharju J. J. Biol. Chem. 278:47685-47693(2003) [PubMed: 14500733] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: Webster.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Strain: C57BL/6J. Tissue: Head.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY313449 mRNA. Translation: AAQ87604.1. AK086468 mRNA. Translation: BAC39675.1. BC082538 mRNA. Translation: AAH82538.1.
IPI	IPI00224951. IPI00406844. IPI00885896.
UniGene	Mm.150294.
3D structure databases
HSSP	HSSP built from PDB template 1TJC based on UniProtKB P13674.
ProteinModelPortal	Q6W3F0.
SMR	Q6W3F0. Positions 177-275, 336-529.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6W3F0.
PTM databases
PhosphoSite	Q6W3F0.
Proteomic databases
PRIDE	Q6W3F0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000077908; ENSMUSP00000077066; ENSMUSG00000051048.
UCSC	uc009imt.2. mouse. uc012fqc.1. mouse.
Organism-specific databases
MGI	MGI:2444049. P4ha3.
Phylogenomic databases
eggNOG	roNOG13726.
GeneTree	ENSGT00390000018885.
HOGENOM	HBG714077.
HOVERGEN	HBG006834.
InParanoid	Q6W3F0.
OrthoDB	EOG4QJRN5.
Enzyme and pathway databases
BRENDA	1.14.11.2. 3474.
Gene expression databases
ArrayExpress	Q6W3F0.
Bgee	Q6W3F0.
Genevestigator	Q6W3F0.
Family and domain databases
InterPro	IPR005123. Oxoglutarate/Fe-dep_oxygenase. IPR006620. Pro_4_hyd_alph. IPR013547. Pro_4_hyd_alph_N. IPR011990. TPR-like_helical. [Graphical view]
Gene3D	G3DSA:1.25.40.10. TPR-like_helical. 1 hit.
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. PF08336. P4Ha_N. 1 hit. [Graphical view]
SMART	SM00702. P4Hc. 1 hit. [Graphical view]
PROSITE	PS51471. FE2OG_OXY. 1 hit. PS50005. TPR. False negative. PS50293. TPR_REGION. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	396752.
SOURCE	Search...

Entry information

Entry name

P4HA3_MOUSE

Accession

Primary (citable) accession number: Q6W3F0
Secondary accession number(s): Q640R2, Q8C3A6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	July 5, 2004
Last modified:	September 21, 2011
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program