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Protein

Bifunctional protein FolD

Gene

folD

Organism
Rhizobium sp. (strain NGR234)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH.UniRule annotation
5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate.UniRule annotation

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei195 – 1951NADPUniRule annotation
Binding sitei236 – 2361NADP; via amide nitrogenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi170 – 1723NADPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Histidine biosynthesis, Methionine biosynthesis, One-carbon metabolism, Purine biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciSFRE394:GBYN-5355-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein FolDUniRule annotation
Including the following 2 domains:
Methylenetetrahydrofolate dehydrogenaseUniRule annotation (EC:1.5.1.5UniRule annotation)
Methenyltetrahydrofolate cyclohydrolaseUniRule annotation (EC:3.5.4.9UniRule annotation)
Gene namesi
Name:folDUniRule annotation
Ordered Locus Names:NGR_b16620
ORF Names:RNGR00186
Encoded oniPlasmid sym pNGR234b0 Publication
OrganismiRhizobium sp. (strain NGR234)
Taxonomic identifieri394 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
Proteomesi
  • UP000001054 Componenti: Plasmid sym pNGR234b

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 307307Bifunctional protein FolDPRO_0000268467Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ6W210.
SMRiQ6W210. Positions 5-293.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000218242.
KOiK01491.
OMAiKYVIAPE.
OrthoDBiEOG6K6VBB.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01576. THF_DHG_CYH.
InterProiIPR016040. NAD(P)-bd_dom.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020867. THF_DH/CycHdrlase_CS.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view]
PfamiPF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view]
PRINTSiPR00085. THFDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00767. THF_DHG_CYH_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6W210-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIGCKLISG REVAESIIDQ VKAHTVALSA NGITPGLAVI IVGSDPASQV
60 70 80 90 100
YVASKGRKAE ECGFLSIKHE LPHDVPERRL LELIDSLNAD PSIHGILVQL
110 120 130 140 150
PLPRHVDSGK VIQAISPQKD IDGFHFINVG KLGTGALETA FVPCTPAGCM
160 170 180 190 200
ILVERIHGKD LSGLSAVVVG RSNIVGKPMA NLLLAANATV TIAHSRTANL
210 220 230 240 250
EVLCRQADIL IAAVGRPEMI RSDWIKPGAT VIDVGINRIA SRDDGGKTRL
260 270 280 290 300
VGDVDFDGAV ERAGAVTPVP GGVGPMTIAM LMANTLRAAC HANDEPAPHF

GSGGVLR
Length:307
Mass (Da):32,056
Last modified:July 5, 2004 - v1
Checksum:iC8419C3CE86C1B52
GO

Sequence cautioni

The sequence ACP23113.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY316746 Genomic DNA. Translation: AAQ87208.1.
CP000874 Genomic DNA. Translation: ACP23113.1. Different initiation.
RefSeqiWP_015887744.1. NC_012586.1.
YP_002823866.1. NC_012586.1.

Genome annotation databases

EnsemblBacteriaiACP23113; ACP23113; NGR_b16620.
GeneIDi7788985.
KEGGirhi:NGR_b16620.
PATRICi32304893. VBIRhiSp122450_2080.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY316746 Genomic DNA. Translation: AAQ87208.1.
CP000874 Genomic DNA. Translation: ACP23113.1. Different initiation.
RefSeqiWP_015887744.1. NC_012586.1.
YP_002823866.1. NC_012586.1.

3D structure databases

ProteinModelPortaliQ6W210.
SMRiQ6W210. Positions 5-293.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACP23113; ACP23113; NGR_b16620.
GeneIDi7788985.
KEGGirhi:NGR_b16620.
PATRICi32304893. VBIRhiSp122450_2080.

Phylogenomic databases

HOGENOMiHOG000218242.
KOiK01491.
OMAiKYVIAPE.
OrthoDBiEOG6K6VBB.

Enzyme and pathway databases

UniPathwayiUPA00193.
BioCyciSFRE394:GBYN-5355-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01576. THF_DHG_CYH.
InterProiIPR016040. NAD(P)-bd_dom.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020867. THF_DH/CycHdrlase_CS.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view]
PfamiPF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view]
PRINTSiPR00085. THFDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00767. THF_DHG_CYH_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "An evolutionary hot spot: the pNGR234b replicon of Rhizobium sp. strain NGR234."
    Streit W.R., Schmitz R.A., Perret X., Staehelin C., Deakin W.J., Raasch C., Liesegang H., Broughton W.J.
    J. Bacteriol. 186:535-542(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NGR234.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NGR234.

Entry informationi

Entry nameiFOLD_RHISN
AccessioniPrimary (citable) accession number: Q6W210
Secondary accession number(s): C3KL27
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: July 5, 2004
Last modified: June 8, 2016
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Plasmid, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.