Q6VZZ7 (OPN5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Opsin-5

Alternative name(s):
G-protein coupled receptor 136
G-protein coupled receptor PGR12
Neuropsin

Gene names

Name:	Opn5
Synonyms:	Gpr136, Pgr12

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Detected in the eye, brain and testis.
Post-translational modification	It is uncertain whether Cys-315 or Cys-316 is palmitoylated.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.

Ontologies

Keywords
Biological process	Sensory transduction
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Ligand	Chromophore
Molecular function	G-protein coupled receptor Photoreceptor protein Receptor Retinal protein Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	phototransduction Inferred from electronic annotation. Source: UniProtKB-KW protein-chromophore linkage Inferred from electronic annotation. Source: UniProtKB-KW visual perception Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	G-protein coupled receptor activity Inferred from electronic annotation. Source: UniProtKB-KW photoreceptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6VZZ7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6VZZ7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     353-353: S → V
     354-377: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 377	377	Opsin-5		PRO_0000197818
Regions
	Topological domain	1 – 33	33	Extracellular Potential
	Transmembrane	34 – 54	21	Helical; Name=1; Potential
	Topological domain	55 – 74	20	Cytoplasmic Potential
	Transmembrane	75 – 95	21	Helical; Name=2; Potential
	Topological domain	96 – 108	13	Extracellular Potential
	Transmembrane	109 – 129	21	Helical; Name=3; Potential
	Topological domain	130 – 150	21	Cytoplasmic Potential
	Transmembrane	151 – 171	21	Helical; Name=4; Potential
	Topological domain	172 – 197	26	Extracellular Potential
	Transmembrane	198 – 218	21	Helical; Name=5; Potential
	Topological domain	219 – 252	34	Cytoplasmic Potential
	Transmembrane	253 – 273	21	Helical; Name=6; Potential
	Topological domain	274 – 288	15	Extracellular Potential
	Transmembrane	289 – 309	21	Helical; Name=7; Potential
	Topological domain	310 – 377	68	Cytoplasmic Potential
Amino acid modifications
	Modified residue	296	1	N6-(retinylidene)lysine
	Lipidation	315	1	S-palmitoyl cysteine Potential
	Lipidation	316	1	S-palmitoyl cysteine Potential
	Glycosylation	4	1	N-linked (GlcNAc...) Potential
	Disulfide bond	106 ↔ 183		Potential
Natural variations
	Alternative sequence	353	1	S → V in isoform 2.		VSP_014041
	Alternative sequence	354 – 377	24	Missing in isoform 2.		VSP_014042
Experimental info
	Sequence conflict	84 – 86	3	Missing in AAP72135. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 26036DC9248B1596	FASTA	377	42,018
	10 20 30 40 50 60 MALNHTALPQ DERLPHYLRD EDPFASKLSW EADLVAGFYL TIIGILSTFG NGYVLYMSSR 70 80 90 100 110 120 RKKKLRPAEI MTINLAVCDL GISVVGKPFT IISCFCHRWV FGWFGCRWYG WAGFFFGCGS 130 140 150 160 170 180 LITMTAVSLD RYLKICYLSY GVWLKRKHAY ICLAVIWAYA SFWTTMPLVG LGDYAPEPFG 190 200 210 220 230 240 TSCTLDWWLA QASGGGQVFI LSILFFCLLL PTAVIVFSYA KIIAKVKSSS KEVAHFDSRI 250 260 270 280 290 300 HSSHVLEVKL TKVAMLICAG FLIAWIPYAV VSVWSAFGRP DSIPIQLSVV PTLLAKSAAM 310 320 330 340 350 360 YNPIIYQVID YRFACCQAGG LRGTKKKSLE DFRLHTVTAV RKSSAVLEIH PESSSRFTSA 370 HVMDGESHSN DGDCGKK
	Isoform 2 [UniParc]. Checksum: 31AA0FC48D7547EE	FASTA	353	39,496
	10 20 30 40 50 60 MALNHTALPQ DERLPHYLRD EDPFASKLSW EADLVAGFYL TIIGILSTFG NGYVLYMSSR 70 80 90 100 110 120 RKKKLRPAEI MTINLAVCDL GISVVGKPFT IISCFCHRWV FGWFGCRWYG WAGFFFGCGS 130 140 150 160 170 180 LITMTAVSLD RYLKICYLSY GVWLKRKHAY ICLAVIWAYA SFWTTMPLVG LGDYAPEPFG 190 200 210 220 230 240 TSCTLDWWLA QASGGGQVFI LSILFFCLLL PTAVIVFSYA KIIAKVKSSS KEVAHFDSRI 250 260 270 280 290 300 HSSHVLEVKL TKVAMLICAG FLIAWIPYAV VSVWSAFGRP DSIPIQLSVV PTLLAKSAAM 310 320 330 340 350 YNPIIYQVID YRFACCQAGG LRGTKKKSLE DFRLHTVTAV RKSSAVLEIH PEV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Seven evolutionarily conserved human rhodopsin G protein-coupled receptors lacking close relatives." Fredriksson R., Hoeglund P.J., Gloriam D.E.I., Lagerstroem M.C., Schioeth H.B. FEBS Lett. 554:381-388(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[3]	"The G protein-coupled receptor repertoires of human and mouse." Vassilatis D.K., Hohmann J.G., Zeng H., Li F., Ranchalis J.E., Mortrud M.T., Brown A., Rodriguez S.S., Weller J.R., Wright A.C., Bergmann J.E., Gaitanaris G.A. Proc. Natl. Acad. Sci. U.S.A. 100:4903-4908(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 154-228.
[4]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 270-377 (ISOFORM 1). Strain: C57BL/6J. Tissue: Spinal cord.
[5]	"Neuropsin (Opn5): a novel opsin identified in mammalian neural tissue." Tarttelin E.E., Bellingham J., Hankins M.W., Foster R.G., Lucas R.J. FEBS Lett. 554:410-416(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION (ISOFORM 1). Strain: C3H.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY288426 mRNA. Translation: AAP72135.1. BC125611 mRNA. Translation: AAI25612.1. BC132010 mRNA. Translation: AAI32011.1. AY255592 mRNA. Translation: AAO85104.1. AK039525 mRNA. Translation: BAC30373.1. AY318865 mRNA. Translation: AAR08201.1. BK001605 Genomic DNA. Translation: DAA01972.1.
IPI	IPI00605624. IPI00620182.
RefSeq	NP_861418.2. NM_181753.4.
UniGene	Mm.184937.
3D structure databases
ProteinModelPortal	Q6VZZ7.
SMR	Q6VZZ7. Positions 30-343.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	Q6VZZ7.
Proteomic databases
PRIDE	Q6VZZ7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000068355; ENSMUSP00000063542; ENSMUSG00000043972.
GeneID	353344.
KEGG	mmu:353344.
UCSC	uc008coq.1. mouse.
Organism-specific databases
CTD	221391.
MGI	MGI:2662912. Opn5.
Phylogenomic databases
eggNOG	NOG145389.
GeneTree	ENSGT00690000101836.
HOGENOM	HOG000115272.
HOVERGEN	HBG058348.
InParanoid	Q059L5.
KO	K08385.
OMA	YLSYGVW.
OrthoDB	EOG46T31T.
Gene expression databases
Bgee	Q6VZZ7.
CleanEx	MM_OPN5.
Genevestigator	Q6VZZ7.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR002962. Peropsin. IPR027430. Retinal_BS. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR01244. PEROPSIN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. PS00238. OPSIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	400417.
SOURCE	Search...

Entry information

Entry name

OPN5_MOUSE

Accession

Primary (citable) accession number: Q6VZZ7
Secondary accession number(s): Q059L5 , Q7TQP1, Q80T51, Q8BYI2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	July 5, 2004
Last modified:	May 29, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program