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Q6VVW9 (CP2R1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vitamin D 25-hydroxylase

EC=1.14.13.15
Alternative name(s):
Cytochrome P450 2R1
Gene names
Name:Cyp2r1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length501 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Has a D-25-hydroxylase activity on both forms of vitamin D, vitamin D2 and D3. Ref.1

Catalytic activity

5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol + 3 NADPH + 3 O2 = (25R)-3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestan-26-oate + 3 NADP+ + 4 H2O.

Cofactor

Heme group By similarity.

Subunit structure

Homodimer By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Tissue specificity

Highly expressed in the liver and testis. Ref.1

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 501501Vitamin D 25-hydroxylase
PRO_0000051779

Sites

Metal binding4481Iron (heme axial ligand) By similarity
Binding site2501Substrate; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6VVW9 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: C1A1BAD71E630A98

FASTA50157,313
        10         20         30         40         50         60 
MLELPGARAC AGALAGALLL LLFVLVVRQL LRQRRPAGFP PGPPRLPFVG NICSLALSAD 

        70         80         90        100        110        120 
LPHVYMRKQS RVYGEIFSLD LGGISTVVLN GYDVVKECLV HQSEIFADRP CLPLFMKMTK 

       130        140        150        160        170        180 
MGGLLNSRYG RGWIDHRRLA VNSFHYFGSG QKSFESKILE ETWSLIDAIE TYKGGPFDLK 

       190        200        210        220        230        240 
QLITNAVSNI TNLILFGERF TYEDTDFQHM IELFSENVEL AASAPVFLYN AFPWIGILPF 

       250        260        270        280        290        300 
GKHQRLFRNA DVVYDFLSRL IEKAAVNRKP HLPHHFVDAY LDEMDQGQND PLSTFSKENL 

       310        320        330        340        350        360 
IFSVGELIIA GTETTTNVLR WAILFMALYP NIQGQVHKEI DLIVGHNRRP SWEYKCKMPY 

       370        380        390        400        410        420 
TEAVLHEVLR FCNIVPLGIF HATSEDAVVR GYSIPKGTTV ITNLYSVHFD EKYWKDPDMF 

       430        440        450        460        470        480 
YPERFLDSNG YFTKKEALIP FSLGRRHCLG EQLARMEMFL FFTSLLQQFH LHFPHELVPN 

       490        500 
LKPRLGMTLQ PQPYLICAER R 

« Hide

References

[1]"De-orphanization of cytochrome P450 2R1: a microsomal vitamin D 25-hydroxylase."
Cheng J.B., Motola D.L., Mangelsdorf D.J., Russell D.W.
J. Biol. Chem. 278:38084-38093(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
Strain: C57BL/6Jx129SvEv.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY323818 mRNA. Translation: AAQ23115.1.
CCDSCCDS21761.1.
RefSeqNP_796356.2. NM_177382.4.
UniGeneMm.108037.
Mm.401952.

3D structure databases

ProteinModelPortalQ6VVW9.
SMRQ6VVW9. Positions 49-501.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ6VVW9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000032908; ENSMUSP00000032908; ENSMUSG00000030670.
GeneID244209.
KEGGmmu:244209.
UCSCuc009jid.1. mouse.

Organism-specific databases

CTD120227.
MGIMGI:2449771. Cyp2r1.

Phylogenomic databases

eggNOGCOG2124.
GeneTreeENSGT00750000117392.
HOGENOMHOG000036991.
HOVERGENHBG015789.
InParanoidQ6VVW9.
KOK07419.
OMAYDVIKEC.
OrthoDBEOG7RBZ85.
PhylomeDBQ6VVW9.
TreeFamTF352043.

Gene expression databases

ArrayExpressQ6VVW9.
BgeeQ6VVW9.
GenevestigatorQ6VVW9.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio386174.
PROQ6VVW9.
SOURCESearch...

Entry information

Entry nameCP2R1_MOUSE
AccessionPrimary (citable) accession number: Q6VVW9
Entry history
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: July 5, 2004
Last modified: July 9, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot