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Q6VVW9

- CP2R1_MOUSE

UniProt

Q6VVW9 - CP2R1_MOUSE

Protein

Vitamin D 25-hydroxylase

Gene

Cyp2r1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Has a D-25-hydroxylase activity on both forms of vitamin D, vitamin D2 and D3.1 Publication

    Catalytic activityi

    5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol + 3 NADPH + 3 O2 = (25R)-3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestan-26-oate + 3 NADP+ + 4 H2O.

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei250 – 2501Substrate; via carbonyl oxygenBy similarity
    Metal bindingi448 – 4481Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. cholestanetriol 26-monooxygenase activity Source: UniProtKB-EC
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro
    4. vitamin D3 25-hydroxylase activity Source: MGI

    GO - Biological processi

    1. calcitriol biosynthetic process from calciol Source: GOC
    2. response to cesium ion Source: Ensembl
    3. response to ionizing radiation Source: Ensembl
    4. vitamin D metabolic process Source: MGI

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding, NADP

    Enzyme and pathway databases

    ReactomeiREACT_213027. Vitamins.
    REACT_218811. Vitamin D (calciferol) metabolism.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vitamin D 25-hydroxylase (EC:1.14.13.15)
    Alternative name(s):
    Cytochrome P450 2R1
    Gene namesi
    Name:Cyp2r1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:2449771. Cyp2r1.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 501501Vitamin D 25-hydroxylasePRO_0000051779Add
    BLAST

    Proteomic databases

    PRIDEiQ6VVW9.

    Expressioni

    Tissue specificityi

    Highly expressed in the liver and testis.1 Publication

    Gene expression databases

    ArrayExpressiQ6VVW9.
    BgeeiQ6VVW9.
    GenevestigatoriQ6VVW9.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ6VVW9.
    SMRiQ6VVW9. Positions 49-501.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    eggNOGiCOG2124.
    GeneTreeiENSGT00750000117392.
    HOGENOMiHOG000036991.
    HOVERGENiHBG015789.
    InParanoidiQ6VVW9.
    KOiK07419.
    OMAiYDVIKEC.
    OrthoDBiEOG7RBZ85.
    PhylomeDBiQ6VVW9.
    TreeFamiTF352043.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6VVW9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLELPGARAC AGALAGALLL LLFVLVVRQL LRQRRPAGFP PGPPRLPFVG    50
    NICSLALSAD LPHVYMRKQS RVYGEIFSLD LGGISTVVLN GYDVVKECLV 100
    HQSEIFADRP CLPLFMKMTK MGGLLNSRYG RGWIDHRRLA VNSFHYFGSG 150
    QKSFESKILE ETWSLIDAIE TYKGGPFDLK QLITNAVSNI TNLILFGERF 200
    TYEDTDFQHM IELFSENVEL AASAPVFLYN AFPWIGILPF GKHQRLFRNA 250
    DVVYDFLSRL IEKAAVNRKP HLPHHFVDAY LDEMDQGQND PLSTFSKENL 300
    IFSVGELIIA GTETTTNVLR WAILFMALYP NIQGQVHKEI DLIVGHNRRP 350
    SWEYKCKMPY TEAVLHEVLR FCNIVPLGIF HATSEDAVVR GYSIPKGTTV 400
    ITNLYSVHFD EKYWKDPDMF YPERFLDSNG YFTKKEALIP FSLGRRHCLG 450
    EQLARMEMFL FFTSLLQQFH LHFPHELVPN LKPRLGMTLQ PQPYLICAER 500
    R 501
    Length:501
    Mass (Da):57,313
    Last modified:July 5, 2004 - v1
    Checksum:iC1A1BAD71E630A98
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY323818 mRNA. Translation: AAQ23115.1.
    CCDSiCCDS21761.1.
    RefSeqiNP_796356.2. NM_177382.4.
    UniGeneiMm.108037.
    Mm.401952.

    Genome annotation databases

    EnsembliENSMUST00000032908; ENSMUSP00000032908; ENSMUSG00000030670.
    GeneIDi244209.
    KEGGimmu:244209.
    UCSCiuc009jid.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY323818 mRNA. Translation: AAQ23115.1 .
    CCDSi CCDS21761.1.
    RefSeqi NP_796356.2. NM_177382.4.
    UniGenei Mm.108037.
    Mm.401952.

    3D structure databases

    ProteinModelPortali Q6VVW9.
    SMRi Q6VVW9. Positions 49-501.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q6VVW9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000032908 ; ENSMUSP00000032908 ; ENSMUSG00000030670 .
    GeneIDi 244209.
    KEGGi mmu:244209.
    UCSCi uc009jid.1. mouse.

    Organism-specific databases

    CTDi 120227.
    MGIi MGI:2449771. Cyp2r1.

    Phylogenomic databases

    eggNOGi COG2124.
    GeneTreei ENSGT00750000117392.
    HOGENOMi HOG000036991.
    HOVERGENi HBG015789.
    InParanoidi Q6VVW9.
    KOi K07419.
    OMAi YDVIKEC.
    OrthoDBi EOG7RBZ85.
    PhylomeDBi Q6VVW9.
    TreeFami TF352043.

    Enzyme and pathway databases

    Reactomei REACT_213027. Vitamins.
    REACT_218811. Vitamin D (calciferol) metabolism.

    Miscellaneous databases

    NextBioi 386174.
    PROi Q6VVW9.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q6VVW9.
    Bgeei Q6VVW9.
    Genevestigatori Q6VVW9.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "De-orphanization of cytochrome P450 2R1: a microsomal vitamin D 25-hydroxylase."
      Cheng J.B., Motola D.L., Mangelsdorf D.J., Russell D.W.
      J. Biol. Chem. 278:38084-38093(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
      Strain: C57BL/6Jx129SvEv.

    Entry informationi

    Entry nameiCP2R1_MOUSE
    AccessioniPrimary (citable) accession number: Q6VVW9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 29, 2005
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3