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Q6VVW9

- CP2R1_MOUSE

UniProt

Q6VVW9 - CP2R1_MOUSE

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Protein
Vitamin D 25-hydroxylase
Gene
Cyp2r1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Has a D-25-hydroxylase activity on both forms of vitamin D, vitamin D2 and D3.1 Publication

Catalytic activityi

5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol + 3 NADPH + 3 O2 = (25R)-3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestan-26-oate + 3 NADP+ + 4 H2O.

Cofactori

Heme group By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei250 – 2501Substrate; via carbonyl oxygen By similarity
Metal bindingi448 – 4481Iron (heme axial ligand) By similarity

GO - Molecular functioni

  1. cholestanetriol 26-monooxygenase activity Source: UniProtKB-EC
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro
  4. vitamin D3 25-hydroxylase activity Source: MGI
Complete GO annotation...

GO - Biological processi

  1. calcitriol biosynthetic process from calciol Source: GOC
  2. response to cesium ion Source: Ensembl
  3. response to ionizing radiation Source: Ensembl
  4. vitamin D metabolic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

ReactomeiREACT_213027. Vitamins.
REACT_218811. Vitamin D (calciferol) metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Vitamin D 25-hydroxylase (EC:1.14.13.15)
Alternative name(s):
Cytochrome P450 2R1
Gene namesi
Name:Cyp2r1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:2449771. Cyp2r1.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 501501Vitamin D 25-hydroxylase
PRO_0000051779Add
BLAST

Proteomic databases

PRIDEiQ6VVW9.

Expressioni

Tissue specificityi

Highly expressed in the liver and testis.1 Publication

Gene expression databases

ArrayExpressiQ6VVW9.
BgeeiQ6VVW9.
GenevestigatoriQ6VVW9.

Interactioni

Subunit structurei

Homodimer By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ6VVW9.
SMRiQ6VVW9. Positions 49-501.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00750000117392.
HOGENOMiHOG000036991.
HOVERGENiHBG015789.
InParanoidiQ6VVW9.
KOiK07419.
OMAiYDVIKEC.
OrthoDBiEOG7RBZ85.
PhylomeDBiQ6VVW9.
TreeFamiTF352043.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6VVW9-1 [UniParc]FASTAAdd to Basket

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MLELPGARAC AGALAGALLL LLFVLVVRQL LRQRRPAGFP PGPPRLPFVG    50
NICSLALSAD LPHVYMRKQS RVYGEIFSLD LGGISTVVLN GYDVVKECLV 100
HQSEIFADRP CLPLFMKMTK MGGLLNSRYG RGWIDHRRLA VNSFHYFGSG 150
QKSFESKILE ETWSLIDAIE TYKGGPFDLK QLITNAVSNI TNLILFGERF 200
TYEDTDFQHM IELFSENVEL AASAPVFLYN AFPWIGILPF GKHQRLFRNA 250
DVVYDFLSRL IEKAAVNRKP HLPHHFVDAY LDEMDQGQND PLSTFSKENL 300
IFSVGELIIA GTETTTNVLR WAILFMALYP NIQGQVHKEI DLIVGHNRRP 350
SWEYKCKMPY TEAVLHEVLR FCNIVPLGIF HATSEDAVVR GYSIPKGTTV 400
ITNLYSVHFD EKYWKDPDMF YPERFLDSNG YFTKKEALIP FSLGRRHCLG 450
EQLARMEMFL FFTSLLQQFH LHFPHELVPN LKPRLGMTLQ PQPYLICAER 500
R 501
Length:501
Mass (Da):57,313
Last modified:July 5, 2004 - v1
Checksum:iC1A1BAD71E630A98
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY323818 mRNA. Translation: AAQ23115.1.
CCDSiCCDS21761.1.
RefSeqiNP_796356.2. NM_177382.4.
UniGeneiMm.108037.
Mm.401952.

Genome annotation databases

EnsembliENSMUST00000032908; ENSMUSP00000032908; ENSMUSG00000030670.
GeneIDi244209.
KEGGimmu:244209.
UCSCiuc009jid.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY323818 mRNA. Translation: AAQ23115.1 .
CCDSi CCDS21761.1.
RefSeqi NP_796356.2. NM_177382.4.
UniGenei Mm.108037.
Mm.401952.

3D structure databases

ProteinModelPortali Q6VVW9.
SMRi Q6VVW9. Positions 49-501.
ModBasei Search...

Proteomic databases

PRIDEi Q6VVW9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000032908 ; ENSMUSP00000032908 ; ENSMUSG00000030670 .
GeneIDi 244209.
KEGGi mmu:244209.
UCSCi uc009jid.1. mouse.

Organism-specific databases

CTDi 120227.
MGIi MGI:2449771. Cyp2r1.

Phylogenomic databases

eggNOGi COG2124.
GeneTreei ENSGT00750000117392.
HOGENOMi HOG000036991.
HOVERGENi HBG015789.
InParanoidi Q6VVW9.
KOi K07419.
OMAi YDVIKEC.
OrthoDBi EOG7RBZ85.
PhylomeDBi Q6VVW9.
TreeFami TF352043.

Enzyme and pathway databases

Reactomei REACT_213027. Vitamins.
REACT_218811. Vitamin D (calciferol) metabolism.

Miscellaneous databases

NextBioi 386174.
PROi Q6VVW9.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q6VVW9.
Bgeei Q6VVW9.
Genevestigatori Q6VVW9.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00463. EP450I.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "De-orphanization of cytochrome P450 2R1: a microsomal vitamin D 25-hydroxylase."
    Cheng J.B., Motola D.L., Mangelsdorf D.J., Russell D.W.
    J. Biol. Chem. 278:38084-38093(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    Strain: C57BL/6Jx129SvEv.

Entry informationi

Entry nameiCP2R1_MOUSE
AccessioniPrimary (citable) accession number: Q6VVW9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: July 5, 2004
Last modified: September 3, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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