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Protein

Vitamin D 25-hydroxylase

Gene

Cyp2r1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Has a D-25-hydroxylase activity on both forms of vitamin D, vitamin D2 and D3.1 Publication

Catalytic activityi

5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol + 3 NADPH + 3 O2 = (25R)-3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestan-26-oate + 3 NADP+ + 4 H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei250 – 2501Substrate; via carbonyl oxygenBy similarity
Metal bindingi448 – 4481Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. cholestanetriol 26-monooxygenase activity Source: UniProtKB-EC
  2. heme binding Source: GO_Central
  3. iron ion binding Source: InterPro
  4. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen Source: GO_Central
  5. oxygen binding Source: GO_Central
  6. steroid hydroxylase activity Source: GO_Central
  7. vitamin D3 25-hydroxylase activity Source: MGI

GO - Biological processi

  1. calcitriol biosynthetic process from calciol Source: GOC
  2. exogenous drug catabolic process Source: GO_Central
  3. organic acid metabolic process Source: GO_Central
  4. vitamin D metabolic process Source: MGI
  5. xenobiotic metabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BRENDAi1.14.13.159. 3474.
ReactomeiREACT_315248. Vitamins.
REACT_352248. Vitamin D (calciferol) metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Vitamin D 25-hydroxylase (EC:1.14.13.15)
Alternative name(s):
Cytochrome P450 2R1
Gene namesi
Name:Cyp2r1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2449771. Cyp2r1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: GO_Central
  2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  3. intracellular membrane-bounded organelle Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 501501Vitamin D 25-hydroxylasePRO_0000051779Add
BLAST

Proteomic databases

PRIDEiQ6VVW9.

Expressioni

Tissue specificityi

Highly expressed in the liver and testis.1 Publication

Gene expression databases

BgeeiQ6VVW9.
ExpressionAtlasiQ6VVW9. baseline and differential.
GenevestigatoriQ6VVW9.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ6VVW9.
SMRiQ6VVW9. Positions 49-501.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118775.
HOGENOMiHOG000036991.
HOVERGENiHBG015789.
InParanoidiQ6VVW9.
KOiK07419.
OMAiNESTRLC.
OrthoDBiEOG7RBZ85.
PhylomeDBiQ6VVW9.
TreeFamiTF352043.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6VVW9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLELPGARAC AGALAGALLL LLFVLVVRQL LRQRRPAGFP PGPPRLPFVG
60 70 80 90 100
NICSLALSAD LPHVYMRKQS RVYGEIFSLD LGGISTVVLN GYDVVKECLV
110 120 130 140 150
HQSEIFADRP CLPLFMKMTK MGGLLNSRYG RGWIDHRRLA VNSFHYFGSG
160 170 180 190 200
QKSFESKILE ETWSLIDAIE TYKGGPFDLK QLITNAVSNI TNLILFGERF
210 220 230 240 250
TYEDTDFQHM IELFSENVEL AASAPVFLYN AFPWIGILPF GKHQRLFRNA
260 270 280 290 300
DVVYDFLSRL IEKAAVNRKP HLPHHFVDAY LDEMDQGQND PLSTFSKENL
310 320 330 340 350
IFSVGELIIA GTETTTNVLR WAILFMALYP NIQGQVHKEI DLIVGHNRRP
360 370 380 390 400
SWEYKCKMPY TEAVLHEVLR FCNIVPLGIF HATSEDAVVR GYSIPKGTTV
410 420 430 440 450
ITNLYSVHFD EKYWKDPDMF YPERFLDSNG YFTKKEALIP FSLGRRHCLG
460 470 480 490 500
EQLARMEMFL FFTSLLQQFH LHFPHELVPN LKPRLGMTLQ PQPYLICAER

R
Length:501
Mass (Da):57,313
Last modified:July 5, 2004 - v1
Checksum:iC1A1BAD71E630A98
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY323818 mRNA. Translation: AAQ23115.1.
CCDSiCCDS21761.1.
RefSeqiNP_796356.2. NM_177382.4.
UniGeneiMm.108037.
Mm.401952.

Genome annotation databases

EnsembliENSMUST00000032908; ENSMUSP00000032908; ENSMUSG00000030670.
GeneIDi244209.
KEGGimmu:244209.
UCSCiuc009jid.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY323818 mRNA. Translation: AAQ23115.1.
CCDSiCCDS21761.1.
RefSeqiNP_796356.2. NM_177382.4.
UniGeneiMm.108037.
Mm.401952.

3D structure databases

ProteinModelPortaliQ6VVW9.
SMRiQ6VVW9. Positions 49-501.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ6VVW9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032908; ENSMUSP00000032908; ENSMUSG00000030670.
GeneIDi244209.
KEGGimmu:244209.
UCSCiuc009jid.1. mouse.

Organism-specific databases

CTDi120227.
MGIiMGI:2449771. Cyp2r1.

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118775.
HOGENOMiHOG000036991.
HOVERGENiHBG015789.
InParanoidiQ6VVW9.
KOiK07419.
OMAiNESTRLC.
OrthoDBiEOG7RBZ85.
PhylomeDBiQ6VVW9.
TreeFamiTF352043.

Enzyme and pathway databases

BRENDAi1.14.13.159. 3474.
ReactomeiREACT_315248. Vitamins.
REACT_352248. Vitamin D (calciferol) metabolism.

Miscellaneous databases

NextBioi386174.
PROiQ6VVW9.
SOURCEiSearch...

Gene expression databases

BgeeiQ6VVW9.
ExpressionAtlasiQ6VVW9. baseline and differential.
GenevestigatoriQ6VVW9.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "De-orphanization of cytochrome P450 2R1: a microsomal vitamin D 25-hydroxylase."
    Cheng J.B., Motola D.L., Mangelsdorf D.J., Russell D.W.
    J. Biol. Chem. 278:38084-38093(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    Strain: C57BL/6Jx129SvEv.

Entry informationi

Entry nameiCP2R1_MOUSE
AccessioniPrimary (citable) accession number: Q6VVW9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: July 5, 2004
Last modified: April 1, 2015
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.