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Protein

Atrial natriuretic peptide receptor 2

Gene

Npr2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for the C-type natriuretic peptide NPPC/CNP hormone. Has guanylate cyclase activity upon binding of its ligand. May play a role in the regulation of skeletal growth.1 Publication

Catalytic activityi

GTP = 3',5'-cyclic GMP + diphosphate.By similarity

GO - Molecular functioni

  • ATP binding Source: Ensembl
  • GTP binding Source: UniProtKB-KW
  • guanylate cyclase activity Source: MGI
  • hormone binding Source: MGI
  • identical protein binding Source: MGI
  • natriuretic peptide receptor activity Source: MGI
  • peptide hormone binding Source: MGI
  • protein kinase activity Source: InterPro

GO - Biological processi

  • bone development Source: MGI
  • cellular response to granulocyte macrophage colony-stimulating factor stimulus Source: Ensembl
  • cGMP biosynthetic process Source: MGI
  • intracellular signal transduction Source: InterPro
  • negative regulation of meiotic cell cycle Source: MGI
  • negative regulation of oocyte maturation Source: MGI
  • ossification Source: UniProtKB-KW
  • receptor guanylyl cyclase signaling pathway Source: UniProtKB
  • single organism reproductive process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Receptor

Keywords - Biological processi

cGMP biosynthesis, Osteogenesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-5578768. Physiological factors.

Names & Taxonomyi

Protein namesi
Recommended name:
Atrial natriuretic peptide receptor 2 (EC:4.6.1.2By similarity)
Alternative name(s):
Atrial natriuretic peptide receptor type B
Short name:
ANP-B
Short name:
ANPR-B
Short name:
NPR-B
Guanylate cyclase B
Short name:
GC-B
Gene namesi
Name:Npr2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:97372. Npr2.

Subcellular locationi

  • Cell membrane By similarity; Single-pass type I membrane protein By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini17 – 458ExtracellularSequence analysisAdd BLAST442
Transmembranei459 – 478HelicalSequence analysisAdd BLAST20
Topological domaini479 – 1047CytoplasmicSequence analysisAdd BLAST569

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice mainly display dwarfism associated with impairment of endochondral ossification, reduced growth of longitudinal vertebra and limb-bone. Self-clasping and priapism that could be due to neuronal disorder are detected. The development of the female reproductive organ is affected.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16Sequence analysisAdd BLAST16
ChainiPRO_000001236517 – 1047Atrial natriuretic peptide receptor 2Add BLAST1031

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi24N-linked (GlcNAc...)Sequence analysis1
Glycosylationi35N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi75 ↔ 101By similarity
Glycosylationi161N-linked (GlcNAc...)Sequence analysis1
Glycosylationi195N-linked (GlcNAc...)Sequence analysis1
Glycosylationi244N-linked (GlcNAc...)Sequence analysis1
Glycosylationi277N-linked (GlcNAc...)Sequence analysis1
Glycosylationi349N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi439InterchainBy similarity
Disulfide bondi448InterchainBy similarity
Modified residuei513PhosphoserineBy similarity1
Modified residuei516PhosphothreonineBy similarity1
Modified residuei518PhosphoserineBy similarity1
Modified residuei522PhosphoserineBy similarity1
Modified residuei523PhosphoserineBy similarity1
Modified residuei526PhosphoserineBy similarity1
Modified residuei529PhosphothreonineBy similarity1

Post-translational modificationi

Phosphorylated. Phosphorylation of the protein kinase-like domain is required for full activation by CNP.By similarity
Glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ6VVW5.
PeptideAtlasiQ6VVW5.
PRIDEiQ6VVW5.

PTM databases

iPTMnetiQ6VVW5.
PhosphoSitePlusiQ6VVW5.

Expressioni

Tissue specificityi

Widely expressed. Expressed in the columnar proliferating and prehypertrophic chondrocyte layers of the tibia.1 Publication

Gene expression databases

BgeeiENSMUSG00000028469.
CleanExiMM_NPR2.
ExpressionAtlasiQ6VVW5. baseline and differential.
GenevisibleiQ6VVW5. MM.

Interactioni

GO - Molecular functioni

  • identical protein binding Source: MGI

Protein-protein interaction databases

BioGridi230934. 2 interactors.
MINTiMINT-4997499.
STRINGi10090.ENSMUSP00000030191.

Structurei

3D structure databases

ProteinModelPortaliQ6VVW5.
SMRiQ6VVW5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini513 – 786Protein kinasePROSITE-ProRule annotationAdd BLAST274
Domaini861 – 991Guanylate cyclasePROSITE-ProRule annotationAdd BLAST131

Sequence similaritiesi

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation
Contains 1 guanylate cyclase domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1023. Eukaryota.
COG2114. LUCA.
GeneTreeiENSGT00760000118959.
HOGENOMiHOG000293307.
HOVERGENiHBG051862.
InParanoidiQ6VVW5.
KOiK12324.
OMAiHQVYTRE.
OrthoDBiEOG091G02QS.
TreeFamiTF106338.

Family and domain databases

Gene3Di3.30.70.1230. 1 hit.
InterProiIPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR001828. ANF_lig-bd_rcpt.
IPR001170. ANPR/GUC.
IPR011009. Kinase-like_dom.
IPR029787. Nucleotide_cyclase.
IPR028082. Peripla_BP_I.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF00211. Guanylate_cyc. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR00255. NATPEPTIDER.
SMARTiSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.
SSF55073. SSF55073. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00458. ANF_RECEPTORS. 1 hit.
PS00452. GUANYLATE_CYCLASE_1. 1 hit.
PS50125. GUANYLATE_CYCLASE_2. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6VVW5-1) [UniParc]FASTAAdd to basket
Also known as: GC-B1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALPSLLLVV AALAGGVRPP GARNLTLAVV LPEHNLSYAW AWPRVGPAVA
60 70 80 90 100
LAVEALGRAL PVDLRFVSSE LDGACSEYLA PLRAVDLKLY HDPDLLLGPG
110 120 130 140 150
CVYPAASVAR FASHWRLPLL TAGAVASGFA AKNEHYRTLV RTGPSAPKLG
160 170 180 190 200
EFVVTLHGHF NWTARAALLY LDARTDDRPH YFTIEGVFEA LQGSNLSVQH
210 220 230 240 250
QVYAREPGGP EQATHFIRAN GRIVYICGPL EMLHEILLQA QRENLTNGDY
260 270 280 290 300
VFFYLDVFGE SLRAGPTRAT GRPWQDNRTQ EQAQALREAF QTVLVITYRE
310 320 330 340 350
PPNPEYQEFQ NRLLIRARED FGVELAPSLM NLIAGCFYDG ILLYAQVLNE
360 370 380 390 400
TIQEGGTRED GLRIVEKMQG RRYHGVTGLV VMDKNNDRET DFVLWAMGDL
410 420 430 440 450
DSGDFQPAAH YSGAEKQIWW TGRPIPWVKG APPLDNPPCA FDLDDPSCDK
460 470 480 490 500
TPLSTLAIVA LGTGVTFIMF GVSSFLIFRK LMLEKELASM LWRIRWEELQ
510 520 530 540 550
FGNSDRYHKG AGSRLTLSLR GSSYGSLMTA HGKYQIFANT GHFKGNVVAI
560 570 580 590 600
KHVNKKRIEL TRQVLFELKH MRDVQFNHLT RFIGACIDPP NICIVTEYCP
610 620 630 640 650
RGSLQDILEN DSINLDWMFR YSLINDLVKG MAFLHNSIIS SHGSLKSSNC
660 670 680 690 700
VVDSRFVLKI TDYGLASFRS TAEPDDSHAL YAKKLWTAPE LLSGNPLPTT
710 720 730 740 750
GMQKADVYSF AIILQEIALR SGPFYLEGLD LSPKEIVQKV RNGQRPYFRP
760 770 780 790 800
SIDRTQLNEE LVLLMERCWA QDPTERPDFG QIKGFIRRFN KEGGTSILDN
810 820 830 840 850
LLLRMEQYAN NLEKLVEERT QAYLEEKRKA EALLYQILPH SVAEQLKRGE
860 870 880 890 900
TVQAEAFDSV TIYFSDIVGF TALSAESTPM QVVTLLNDLY TCFDAIIDNF
910 920 930 940 950
DVYKVETIGD AYMVVSGLPG RNGQRHAPEI ARMALALLDA VSSFRIRHRP
960 970 980 990 1000
HDQLRLRIGV HTGPVCAGVV GLKMPRYCLF GDTVNTASRM ESNGQALKIH
1010 1020 1030 1040
VSSTTKDALD ELGCFQLELR GDVEMKGKGK MRTYWLLGEQ KGPPGLL
Length:1,047
Mass (Da):117,060
Last modified:July 27, 2011 - v2
Checksum:i34E6C00E561D6D87
GO
Isoform 2 (identifier: Q6VVW5-2) [UniParc]FASTAAdd to basket
Also known as: GC-B2

The sequence of this isoform differs from the canonical sequence as follows:
     520-544: Missing.

Note: Binds ligand, but no cyclase activation.
Show »
Length:1,022
Mass (Da):114,319
Checksum:iC8CD69A59CDF1C4C
GO
Isoform 3 (identifier: Q6VVW5-3) [UniParc]FASTAAdd to basket
Also known as: GC-B3

The sequence of this isoform differs from the canonical sequence as follows:
     407-418: PAAHYSGAEKQI → VMGERTGRRDWS
     419-1047: Missing.

Note: Fail to bind the ligand.
Show »
Length:418
Mass (Da):46,443
Checksum:i8F6E16CCD6D17C2E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti990M → K in AAQ02634 (PubMed:14514678).Curated1
Sequence conflicti990M → K in AAQ02635 (PubMed:14514678).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_013584407 – 418PAAHY…AEKQI → VMGERTGRRDWS in isoform 3. CuratedAdd BLAST12
Alternative sequenceiVSP_013585419 – 1047Missing in isoform 3. CuratedAdd BLAST629
Alternative sequenceiVSP_013583520 – 544Missing in isoform 2. CuratedAdd BLAST25

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY323833, AY323832 Genomic DNA. Translation: AAQ02634.1.
AY323833, AY323832 Genomic DNA. Translation: AAQ02635.1.
AY323833, AY323832 Genomic DNA. Translation: AAQ02636.1.
AL732626 Genomic DNA. Translation: CAM17047.1.
CH466565 Genomic DNA. Translation: EDL02456.1.
BC042470 mRNA. Translation: AAH42470.1.
CCDSiCCDS18105.1. [Q6VVW5-1]
RefSeqiNP_776149.1. NM_173788.3. [Q6VVW5-1]
XP_006537896.1. XM_006537833.3. [Q6VVW5-2]
UniGeneiMm.103477.

Genome annotation databases

EnsembliENSMUST00000030191; ENSMUSP00000030191; ENSMUSG00000028469. [Q6VVW5-1]
GeneIDi230103.
KEGGimmu:230103.
UCSCiuc008sqm.1. mouse. [Q6VVW5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY323833, AY323832 Genomic DNA. Translation: AAQ02634.1.
AY323833, AY323832 Genomic DNA. Translation: AAQ02635.1.
AY323833, AY323832 Genomic DNA. Translation: AAQ02636.1.
AL732626 Genomic DNA. Translation: CAM17047.1.
CH466565 Genomic DNA. Translation: EDL02456.1.
BC042470 mRNA. Translation: AAH42470.1.
CCDSiCCDS18105.1. [Q6VVW5-1]
RefSeqiNP_776149.1. NM_173788.3. [Q6VVW5-1]
XP_006537896.1. XM_006537833.3. [Q6VVW5-2]
UniGeneiMm.103477.

3D structure databases

ProteinModelPortaliQ6VVW5.
SMRiQ6VVW5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230934. 2 interactors.
MINTiMINT-4997499.
STRINGi10090.ENSMUSP00000030191.

PTM databases

iPTMnetiQ6VVW5.
PhosphoSitePlusiQ6VVW5.

Proteomic databases

PaxDbiQ6VVW5.
PeptideAtlasiQ6VVW5.
PRIDEiQ6VVW5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030191; ENSMUSP00000030191; ENSMUSG00000028469. [Q6VVW5-1]
GeneIDi230103.
KEGGimmu:230103.
UCSCiuc008sqm.1. mouse. [Q6VVW5-1]

Organism-specific databases

CTDi4882.
MGIiMGI:97372. Npr2.

Phylogenomic databases

eggNOGiKOG1023. Eukaryota.
COG2114. LUCA.
GeneTreeiENSGT00760000118959.
HOGENOMiHOG000293307.
HOVERGENiHBG051862.
InParanoidiQ6VVW5.
KOiK12324.
OMAiHQVYTRE.
OrthoDBiEOG091G02QS.
TreeFamiTF106338.

Enzyme and pathway databases

ReactomeiR-MMU-5578768. Physiological factors.

Miscellaneous databases

PROiQ6VVW5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028469.
CleanExiMM_NPR2.
ExpressionAtlasiQ6VVW5. baseline and differential.
GenevisibleiQ6VVW5. MM.

Family and domain databases

Gene3Di3.30.70.1230. 1 hit.
InterProiIPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR001828. ANF_lig-bd_rcpt.
IPR001170. ANPR/GUC.
IPR011009. Kinase-like_dom.
IPR029787. Nucleotide_cyclase.
IPR028082. Peripla_BP_I.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF00211. Guanylate_cyc. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR00255. NATPEPTIDER.
SMARTiSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.
SSF55073. SSF55073. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00458. ANF_RECEPTORS. 1 hit.
PS00452. GUANYLATE_CYCLASE_1. 1 hit.
PS50125. GUANYLATE_CYCLASE_2. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiANPRB_MOUSE
AccessioniPrimary (citable) accession number: Q6VVW5
Secondary accession number(s): B1AWI8
, Q6VVW3, Q6VVW4, Q8CGA9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.