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Q6VVW5

- ANPRB_MOUSE

UniProt

Q6VVW5 - ANPRB_MOUSE

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Protein

Atrial natriuretic peptide receptor 2

Gene

Npr2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Receptor for the C-type natriuretic peptide NPPC/CNP hormone. Has guanylate cyclase activity upon binding of its ligand. May play a role in the regulation of skeletal growth.1 Publication

Catalytic activityi

GTP = 3',5'-cyclic GMP + diphosphate.

GO - Molecular functioni

  1. ATP binding Source: Ensembl
  2. GTP binding Source: UniProtKB-KW
  3. guanylate cyclase activity Source: MGI
  4. identical protein binding Source: MGI
  5. natriuretic peptide receptor activity Source: MGI
  6. peptide hormone binding Source: Ensembl
  7. protein kinase activity Source: InterPro

GO - Biological processi

  1. bone development Source: MGI
  2. cellular response to granulocyte macrophage colony-stimulating factor stimulus Source: Ensembl
  3. cGMP biosynthetic process Source: MGI
  4. intracellular signal transduction Source: InterPro
  5. negative regulation of meiotic cell cycle Source: MGI
  6. negative regulation of oocyte maturation Source: MGI
  7. ossification Source: UniProtKB-KW
  8. receptor guanylyl cyclase signaling pathway Source: Ensembl
  9. single organism reproductive process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Receptor

Keywords - Biological processi

cGMP biosynthesis, Osteogenesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Atrial natriuretic peptide receptor 2 (EC:4.6.1.2)
Alternative name(s):
Atrial natriuretic peptide receptor type B
Short name:
ANP-B
Short name:
ANPR-B
Short name:
NPR-B
Guanylate cyclase B
Short name:
GC-B
Gene namesi
Name:Npr2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:97372. Npr2.

Subcellular locationi

Membrane By similarity; Single-pass type I membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini17 – 458442ExtracellularSequence AnalysisAdd
BLAST
Transmembranei459 – 47820HelicalSequence AnalysisAdd
BLAST
Topological domaini479 – 1047569CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Mice mainly display dwarfism associated with impairment of endochondral ossification, reduced growth of longitudinal vertebra and limb-bone. Self-clasping and priapism that could be due to neuronal disorder are detected. The development of the female reproductive organ is affected.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616Sequence AnalysisAdd
BLAST
Chaini17 – 10471031Atrial natriuretic peptide receptor 2PRO_0000012365Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi24 – 241N-linked (GlcNAc...)Sequence Analysis
Glycosylationi35 – 351N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi75 ↔ 101By similarity
Glycosylationi161 – 1611N-linked (GlcNAc...)Sequence Analysis
Glycosylationi195 – 1951N-linked (GlcNAc...)Sequence Analysis
Glycosylationi244 – 2441N-linked (GlcNAc...)Sequence Analysis
Glycosylationi277 – 2771N-linked (GlcNAc...)Sequence Analysis
Glycosylationi349 – 3491N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi439 – 439InterchainBy similarity
Disulfide bondi448 – 448InterchainBy similarity
Modified residuei513 – 5131PhosphoserineBy similarity
Modified residuei516 – 5161PhosphothreonineBy similarity
Modified residuei518 – 5181PhosphoserineBy similarity
Modified residuei523 – 5231PhosphoserineBy similarity
Modified residuei526 – 5261PhosphoserineBy similarity
Modified residuei529 – 5291PhosphothreonineBy similarity

Post-translational modificationi

Phosphorylation of the protein kinase-like domain is required for full activation by CNP.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ6VVW5.
PaxDbiQ6VVW5.
PRIDEiQ6VVW5.

PTM databases

PhosphoSiteiQ6VVW5.

Expressioni

Tissue specificityi

Widely expressed. Expressed in the columnar proliferating and prehypertrophic chondrocyte layers of the tibia.1 Publication

Gene expression databases

BgeeiQ6VVW5.
CleanExiMM_NPR2.
ExpressionAtlasiQ6VVW5. baseline and differential.
GenevestigatoriQ6VVW5.

Interactioni

Protein-protein interaction databases

BioGridi230934. 2 interactions.
MINTiMINT-4997499.

Structurei

3D structure databases

ProteinModelPortaliQ6VVW5.
SMRiQ6VVW5. Positions 24-442, 503-832, 852-1025.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini513 – 786274Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini861 – 991131Guanylate cyclasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation
Contains 1 guanylate cyclase domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2114.
GeneTreeiENSGT00760000118959.
HOGENOMiHOG000293307.
HOVERGENiHBG051862.
InParanoidiQ6VVW5.
KOiK12324.
OMAiAAKSEHY.
OrthoDBiEOG7Z69BJ.
TreeFamiTF106338.

Family and domain databases

Gene3Di3.30.70.1230. 1 hit.
InterProiIPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR001828. ANF_lig-bd_rcpt.
IPR011009. Kinase-like_dom.
IPR001170. Ntpep_rcpt.
IPR029787. Nucleotide_cyclase.
IPR028082. Peripla_BP_I.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF00211. Guanylate_cyc. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR00255. NATPEPTIDER.
SMARTiSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.
SSF55073. SSF55073. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00458. ANF_RECEPTORS. 1 hit.
PS00452. GUANYLATE_CYCLASE_1. 1 hit.
PS50125. GUANYLATE_CYCLASE_2. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q6VVW5-1) [UniParc]FASTAAdd to Basket

Also known as: GC-B1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALPSLLLVV AALAGGVRPP GARNLTLAVV LPEHNLSYAW AWPRVGPAVA
60 70 80 90 100
LAVEALGRAL PVDLRFVSSE LDGACSEYLA PLRAVDLKLY HDPDLLLGPG
110 120 130 140 150
CVYPAASVAR FASHWRLPLL TAGAVASGFA AKNEHYRTLV RTGPSAPKLG
160 170 180 190 200
EFVVTLHGHF NWTARAALLY LDARTDDRPH YFTIEGVFEA LQGSNLSVQH
210 220 230 240 250
QVYAREPGGP EQATHFIRAN GRIVYICGPL EMLHEILLQA QRENLTNGDY
260 270 280 290 300
VFFYLDVFGE SLRAGPTRAT GRPWQDNRTQ EQAQALREAF QTVLVITYRE
310 320 330 340 350
PPNPEYQEFQ NRLLIRARED FGVELAPSLM NLIAGCFYDG ILLYAQVLNE
360 370 380 390 400
TIQEGGTRED GLRIVEKMQG RRYHGVTGLV VMDKNNDRET DFVLWAMGDL
410 420 430 440 450
DSGDFQPAAH YSGAEKQIWW TGRPIPWVKG APPLDNPPCA FDLDDPSCDK
460 470 480 490 500
TPLSTLAIVA LGTGVTFIMF GVSSFLIFRK LMLEKELASM LWRIRWEELQ
510 520 530 540 550
FGNSDRYHKG AGSRLTLSLR GSSYGSLMTA HGKYQIFANT GHFKGNVVAI
560 570 580 590 600
KHVNKKRIEL TRQVLFELKH MRDVQFNHLT RFIGACIDPP NICIVTEYCP
610 620 630 640 650
RGSLQDILEN DSINLDWMFR YSLINDLVKG MAFLHNSIIS SHGSLKSSNC
660 670 680 690 700
VVDSRFVLKI TDYGLASFRS TAEPDDSHAL YAKKLWTAPE LLSGNPLPTT
710 720 730 740 750
GMQKADVYSF AIILQEIALR SGPFYLEGLD LSPKEIVQKV RNGQRPYFRP
760 770 780 790 800
SIDRTQLNEE LVLLMERCWA QDPTERPDFG QIKGFIRRFN KEGGTSILDN
810 820 830 840 850
LLLRMEQYAN NLEKLVEERT QAYLEEKRKA EALLYQILPH SVAEQLKRGE
860 870 880 890 900
TVQAEAFDSV TIYFSDIVGF TALSAESTPM QVVTLLNDLY TCFDAIIDNF
910 920 930 940 950
DVYKVETIGD AYMVVSGLPG RNGQRHAPEI ARMALALLDA VSSFRIRHRP
960 970 980 990 1000
HDQLRLRIGV HTGPVCAGVV GLKMPRYCLF GDTVNTASRM ESNGQALKIH
1010 1020 1030 1040
VSSTTKDALD ELGCFQLELR GDVEMKGKGK MRTYWLLGEQ KGPPGLL
Length:1,047
Mass (Da):117,060
Last modified:July 27, 2011 - v2
Checksum:i34E6C00E561D6D87
GO
Isoform 2 (identifier: Q6VVW5-2) [UniParc]FASTAAdd to Basket

Also known as: GC-B2

The sequence of this isoform differs from the canonical sequence as follows:
     520-544: Missing.

Note: Binds ligand, but no cyclase activation.

Show »
Length:1,022
Mass (Da):114,319
Checksum:iC8CD69A59CDF1C4C
GO
Isoform 3 (identifier: Q6VVW5-3) [UniParc]FASTAAdd to Basket

Also known as: GC-B3

The sequence of this isoform differs from the canonical sequence as follows:
     407-418: PAAHYSGAEKQI → VMGERTGRRDWS
     419-1047: Missing.

Note: Fail to bind the ligand.

Show »
Length:418
Mass (Da):46,443
Checksum:i8F6E16CCD6D17C2E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti990 – 9901M → K in AAQ02634. (PubMed:14514678)Curated
Sequence conflicti990 – 9901M → K in AAQ02635. (PubMed:14514678)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei407 – 41812PAAHY…AEKQI → VMGERTGRRDWS in isoform 3. CuratedVSP_013584Add
BLAST
Alternative sequencei419 – 1047629Missing in isoform 3. CuratedVSP_013585Add
BLAST
Alternative sequencei520 – 54425Missing in isoform 2. CuratedVSP_013583Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY323833, AY323832 Genomic DNA. Translation: AAQ02634.1.
AY323833, AY323832 Genomic DNA. Translation: AAQ02635.1.
AY323833, AY323832 Genomic DNA. Translation: AAQ02636.1.
AL732626 Genomic DNA. Translation: CAM17047.1.
CH466565 Genomic DNA. Translation: EDL02456.1.
BC042470 mRNA. Translation: AAH42470.1.
CCDSiCCDS18105.1. [Q6VVW5-1]
RefSeqiNP_776149.1. NM_173788.3. [Q6VVW5-1]
XP_006537896.1. XM_006537833.1. [Q6VVW5-2]
UniGeneiMm.103477.

Genome annotation databases

EnsembliENSMUST00000030191; ENSMUSP00000030191; ENSMUSG00000028469. [Q6VVW5-1]
GeneIDi230103.
KEGGimmu:230103.
UCSCiuc008sqm.1. mouse. [Q6VVW5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY323833 , AY323832 Genomic DNA. Translation: AAQ02634.1 .
AY323833 , AY323832 Genomic DNA. Translation: AAQ02635.1 .
AY323833 , AY323832 Genomic DNA. Translation: AAQ02636.1 .
AL732626 Genomic DNA. Translation: CAM17047.1 .
CH466565 Genomic DNA. Translation: EDL02456.1 .
BC042470 mRNA. Translation: AAH42470.1 .
CCDSi CCDS18105.1. [Q6VVW5-1 ]
RefSeqi NP_776149.1. NM_173788.3. [Q6VVW5-1 ]
XP_006537896.1. XM_006537833.1. [Q6VVW5-2 ]
UniGenei Mm.103477.

3D structure databases

ProteinModelPortali Q6VVW5.
SMRi Q6VVW5. Positions 24-442, 503-832, 852-1025.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 230934. 2 interactions.
MINTi MINT-4997499.

PTM databases

PhosphoSitei Q6VVW5.

Proteomic databases

MaxQBi Q6VVW5.
PaxDbi Q6VVW5.
PRIDEi Q6VVW5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000030191 ; ENSMUSP00000030191 ; ENSMUSG00000028469 . [Q6VVW5-1 ]
GeneIDi 230103.
KEGGi mmu:230103.
UCSCi uc008sqm.1. mouse. [Q6VVW5-1 ]

Organism-specific databases

CTDi 4882.
MGIi MGI:97372. Npr2.

Phylogenomic databases

eggNOGi COG2114.
GeneTreei ENSGT00760000118959.
HOGENOMi HOG000293307.
HOVERGENi HBG051862.
InParanoidi Q6VVW5.
KOi K12324.
OMAi AAKSEHY.
OrthoDBi EOG7Z69BJ.
TreeFami TF106338.

Miscellaneous databases

NextBioi 379796.
PROi Q6VVW5.
SOURCEi Search...

Gene expression databases

Bgeei Q6VVW5.
CleanExi MM_NPR2.
ExpressionAtlasi Q6VVW5. baseline and differential.
Genevestigatori Q6VVW5.

Family and domain databases

Gene3Di 3.30.70.1230. 1 hit.
InterProi IPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR001828. ANF_lig-bd_rcpt.
IPR011009. Kinase-like_dom.
IPR001170. Ntpep_rcpt.
IPR029787. Nucleotide_cyclase.
IPR028082. Peripla_BP_I.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view ]
Pfami PF01094. ANF_receptor. 1 hit.
PF00211. Guanylate_cyc. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view ]
PRINTSi PR00255. NATPEPTIDER.
SMARTi SM00044. CYCc. 1 hit.
[Graphical view ]
SUPFAMi SSF53822. SSF53822. 1 hit.
SSF55073. SSF55073. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS00458. ANF_RECEPTORS. 1 hit.
PS00452. GUANYLATE_CYCLASE_1. 1 hit.
PS50125. GUANYLATE_CYCLASE_2. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Regulation of the guanylyl cyclase-B receptor by alternative splicing."
    Tamura N., Garbers D.L.
    J. Biol. Chem. 278:48880-48889(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: 129S6/SvEvTac.
    Tissue: Spleen.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Liver.
  5. "Critical roles of the guanylyl cyclase B receptor in endochondral ossification and development of female reproductive organs."
    Tamura N., Doolittle L.K., Hammer R.E., Shelton J.M., Richardson J.A., Garbers D.L.
    Proc. Natl. Acad. Sci. U.S.A. 101:17300-17305(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.

Entry informationi

Entry nameiANPRB_MOUSE
AccessioniPrimary (citable) accession number: Q6VVW5
Secondary accession number(s): B1AWI8
, Q6VVW3, Q6VVW4, Q8CGA9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: July 27, 2011
Last modified: November 26, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3