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Q6VVW5

- ANPRB_MOUSE

UniProt

Q6VVW5 - ANPRB_MOUSE

Protein

Atrial natriuretic peptide receptor 2

Gene

Npr2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Receptor for the C-type natriuretic peptide NPPC/CNP hormone. Has guanylate cyclase activity upon binding of its ligand. May play a role in the regulation of skeletal growth.1 Publication

    Catalytic activityi

    GTP = 3',5'-cyclic GMP + diphosphate.

    GO - Molecular functioni

    1. ATP binding Source: Ensembl
    2. GTP binding Source: UniProtKB-KW
    3. guanylate cyclase activity Source: MGI
    4. identical protein binding Source: MGI
    5. natriuretic peptide receptor activity Source: MGI
    6. peptide hormone binding Source: Ensembl
    7. protein kinase activity Source: InterPro

    GO - Biological processi

    1. bone development Source: MGI
    2. cellular response to granulocyte macrophage colony-stimulating factor stimulus Source: Ensembl
    3. cGMP biosynthetic process Source: MGI
    4. intracellular signal transduction Source: InterPro
    5. negative regulation of meiotic cell cycle Source: MGI
    6. negative regulation of oocyte maturation Source: MGI
    7. ossification Source: UniProtKB-KW
    8. receptor guanylyl cyclase signaling pathway Source: Ensembl
    9. single organism reproductive process Source: MGI

    Keywords - Molecular functioni

    Lyase, Receptor

    Keywords - Biological processi

    cGMP biosynthesis, Osteogenesis

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Atrial natriuretic peptide receptor 2 (EC:4.6.1.2)
    Alternative name(s):
    Atrial natriuretic peptide receptor type B
    Short name:
    ANP-B
    Short name:
    ANPR-B
    Short name:
    NPR-B
    Guanylate cyclase B
    Short name:
    GC-B
    Gene namesi
    Name:Npr2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:97372. Npr2.

    Subcellular locationi

    Membrane By similarity; Single-pass type I membrane protein By similarity

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: MGI

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Mice mainly display dwarfism associated with impairment of endochondral ossification, reduced growth of longitudinal vertebra and limb-bone. Self-clasping and priapism that could be due to neuronal disorder are detected. The development of the female reproductive organ is affected.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1616Sequence AnalysisAdd
    BLAST
    Chaini17 – 10471031Atrial natriuretic peptide receptor 2PRO_0000012365Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi24 – 241N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi35 – 351N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi75 ↔ 101By similarity
    Glycosylationi161 – 1611N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi195 – 1951N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi244 – 2441N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi277 – 2771N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi349 – 3491N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi439 – 439InterchainBy similarity
    Disulfide bondi448 – 448InterchainBy similarity
    Modified residuei513 – 5131PhosphoserineBy similarity
    Modified residuei516 – 5161PhosphothreonineBy similarity
    Modified residuei518 – 5181PhosphoserineBy similarity
    Modified residuei523 – 5231PhosphoserineBy similarity
    Modified residuei526 – 5261PhosphoserineBy similarity
    Modified residuei529 – 5291PhosphothreonineBy similarity

    Post-translational modificationi

    Phosphorylation of the protein kinase-like domain is required for full activation by CNP.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiQ6VVW5.
    PRIDEiQ6VVW5.

    PTM databases

    PhosphoSiteiQ6VVW5.

    Expressioni

    Tissue specificityi

    Widely expressed. Expressed in the columnar proliferating and prehypertrophic chondrocyte layers of the tibia.1 Publication

    Gene expression databases

    ArrayExpressiQ6VVW5.
    BgeeiQ6VVW5.
    CleanExiMM_NPR2.
    GenevestigatoriQ6VVW5.

    Interactioni

    Protein-protein interaction databases

    BioGridi230934. 2 interactions.
    MINTiMINT-4997499.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6VVW5.
    SMRiQ6VVW5. Positions 24-442, 503-832, 854-1036.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini17 – 458442ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini479 – 1047569CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei459 – 47820HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini513 – 786274Protein kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini861 – 991131Guanylate cyclasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation
    Contains 1 guanylate cyclase domain.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG2114.
    GeneTreeiENSGT00710000106571.
    HOGENOMiHOG000293307.
    HOVERGENiHBG051862.
    InParanoidiB1AWI8.
    KOiK12324.
    OMAiAAKSEHY.
    OrthoDBiEOG7Z69BJ.
    TreeFamiTF106338.

    Family and domain databases

    Gene3Di3.30.70.1230. 1 hit.
    InterProiIPR001054. A/G_cyclase.
    IPR018297. A/G_cyclase_CS.
    IPR001828. ANF_lig-bd_rcpt.
    IPR011009. Kinase-like_dom.
    IPR001170. Ntpep_rcpt.
    IPR028082. Peripla_BP_I.
    IPR000719. Prot_kinase_dom.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    [Graphical view]
    PfamiPF01094. ANF_receptor. 1 hit.
    PF00211. Guanylate_cyc. 1 hit.
    PF07714. Pkinase_Tyr. 1 hit.
    [Graphical view]
    PRINTSiPR00255. NATPEPTIDER.
    SMARTiSM00044. CYCc. 1 hit.
    [Graphical view]
    SUPFAMiSSF53822. SSF53822. 1 hit.
    SSF55073. SSF55073. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS00458. ANF_RECEPTORS. 1 hit.
    PS00452. GUANYLATE_CYCLASE_1. 1 hit.
    PS50125. GUANYLATE_CYCLASE_2. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q6VVW5-1) [UniParc]FASTAAdd to Basket

    Also known as: GC-B1

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MALPSLLLVV AALAGGVRPP GARNLTLAVV LPEHNLSYAW AWPRVGPAVA     50
    LAVEALGRAL PVDLRFVSSE LDGACSEYLA PLRAVDLKLY HDPDLLLGPG 100
    CVYPAASVAR FASHWRLPLL TAGAVASGFA AKNEHYRTLV RTGPSAPKLG 150
    EFVVTLHGHF NWTARAALLY LDARTDDRPH YFTIEGVFEA LQGSNLSVQH 200
    QVYAREPGGP EQATHFIRAN GRIVYICGPL EMLHEILLQA QRENLTNGDY 250
    VFFYLDVFGE SLRAGPTRAT GRPWQDNRTQ EQAQALREAF QTVLVITYRE 300
    PPNPEYQEFQ NRLLIRARED FGVELAPSLM NLIAGCFYDG ILLYAQVLNE 350
    TIQEGGTRED GLRIVEKMQG RRYHGVTGLV VMDKNNDRET DFVLWAMGDL 400
    DSGDFQPAAH YSGAEKQIWW TGRPIPWVKG APPLDNPPCA FDLDDPSCDK 450
    TPLSTLAIVA LGTGVTFIMF GVSSFLIFRK LMLEKELASM LWRIRWEELQ 500
    FGNSDRYHKG AGSRLTLSLR GSSYGSLMTA HGKYQIFANT GHFKGNVVAI 550
    KHVNKKRIEL TRQVLFELKH MRDVQFNHLT RFIGACIDPP NICIVTEYCP 600
    RGSLQDILEN DSINLDWMFR YSLINDLVKG MAFLHNSIIS SHGSLKSSNC 650
    VVDSRFVLKI TDYGLASFRS TAEPDDSHAL YAKKLWTAPE LLSGNPLPTT 700
    GMQKADVYSF AIILQEIALR SGPFYLEGLD LSPKEIVQKV RNGQRPYFRP 750
    SIDRTQLNEE LVLLMERCWA QDPTERPDFG QIKGFIRRFN KEGGTSILDN 800
    LLLRMEQYAN NLEKLVEERT QAYLEEKRKA EALLYQILPH SVAEQLKRGE 850
    TVQAEAFDSV TIYFSDIVGF TALSAESTPM QVVTLLNDLY TCFDAIIDNF 900
    DVYKVETIGD AYMVVSGLPG RNGQRHAPEI ARMALALLDA VSSFRIRHRP 950
    HDQLRLRIGV HTGPVCAGVV GLKMPRYCLF GDTVNTASRM ESNGQALKIH 1000
    VSSTTKDALD ELGCFQLELR GDVEMKGKGK MRTYWLLGEQ KGPPGLL 1047
    Length:1,047
    Mass (Da):117,060
    Last modified:July 27, 2011 - v2
    Checksum:i34E6C00E561D6D87
    GO
    Isoform 2 (identifier: Q6VVW5-2) [UniParc]FASTAAdd to Basket

    Also known as: GC-B2

    The sequence of this isoform differs from the canonical sequence as follows:
         520-544: Missing.

    Note: Binds ligand, but no cyclase activation.

    Show »
    Length:1,022
    Mass (Da):114,319
    Checksum:iC8CD69A59CDF1C4C
    GO
    Isoform 3 (identifier: Q6VVW5-3) [UniParc]FASTAAdd to Basket

    Also known as: GC-B3

    The sequence of this isoform differs from the canonical sequence as follows:
         407-418: PAAHYSGAEKQI → VMGERTGRRDWS
         419-1047: Missing.

    Note: Fail to bind the ligand.

    Show »
    Length:418
    Mass (Da):46,443
    Checksum:i8F6E16CCD6D17C2E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti990 – 9901M → K in AAQ02634. (PubMed:14514678)Curated
    Sequence conflicti990 – 9901M → K in AAQ02635. (PubMed:14514678)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei407 – 41812PAAHY…AEKQI → VMGERTGRRDWS in isoform 3. CuratedVSP_013584Add
    BLAST
    Alternative sequencei419 – 1047629Missing in isoform 3. CuratedVSP_013585Add
    BLAST
    Alternative sequencei520 – 54425Missing in isoform 2. CuratedVSP_013583Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY323833, AY323832 Genomic DNA. Translation: AAQ02634.1.
    AY323833, AY323832 Genomic DNA. Translation: AAQ02635.1.
    AY323833, AY323832 Genomic DNA. Translation: AAQ02636.1.
    AL732626 Genomic DNA. Translation: CAM17047.1.
    CH466565 Genomic DNA. Translation: EDL02456.1.
    BC042470 mRNA. Translation: AAH42470.1.
    CCDSiCCDS18105.1. [Q6VVW5-1]
    RefSeqiNP_776149.1. NM_173788.3. [Q6VVW5-1]
    XP_006537896.1. XM_006537833.1. [Q6VVW5-2]
    UniGeneiMm.103477.

    Genome annotation databases

    EnsembliENSMUST00000030191; ENSMUSP00000030191; ENSMUSG00000028469. [Q6VVW5-1]
    GeneIDi230103.
    KEGGimmu:230103.
    UCSCiuc008sqm.1. mouse. [Q6VVW5-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY323833 , AY323832 Genomic DNA. Translation: AAQ02634.1 .
    AY323833 , AY323832 Genomic DNA. Translation: AAQ02635.1 .
    AY323833 , AY323832 Genomic DNA. Translation: AAQ02636.1 .
    AL732626 Genomic DNA. Translation: CAM17047.1 .
    CH466565 Genomic DNA. Translation: EDL02456.1 .
    BC042470 mRNA. Translation: AAH42470.1 .
    CCDSi CCDS18105.1. [Q6VVW5-1 ]
    RefSeqi NP_776149.1. NM_173788.3. [Q6VVW5-1 ]
    XP_006537896.1. XM_006537833.1. [Q6VVW5-2 ]
    UniGenei Mm.103477.

    3D structure databases

    ProteinModelPortali Q6VVW5.
    SMRi Q6VVW5. Positions 24-442, 503-832, 854-1036.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 230934. 2 interactions.
    MINTi MINT-4997499.

    PTM databases

    PhosphoSitei Q6VVW5.

    Proteomic databases

    PaxDbi Q6VVW5.
    PRIDEi Q6VVW5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000030191 ; ENSMUSP00000030191 ; ENSMUSG00000028469 . [Q6VVW5-1 ]
    GeneIDi 230103.
    KEGGi mmu:230103.
    UCSCi uc008sqm.1. mouse. [Q6VVW5-1 ]

    Organism-specific databases

    CTDi 4882.
    MGIi MGI:97372. Npr2.

    Phylogenomic databases

    eggNOGi COG2114.
    GeneTreei ENSGT00710000106571.
    HOGENOMi HOG000293307.
    HOVERGENi HBG051862.
    InParanoidi B1AWI8.
    KOi K12324.
    OMAi AAKSEHY.
    OrthoDBi EOG7Z69BJ.
    TreeFami TF106338.

    Miscellaneous databases

    ChiTaRSi NPR2. mouse.
    NextBioi 379796.
    PROi Q6VVW5.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q6VVW5.
    Bgeei Q6VVW5.
    CleanExi MM_NPR2.
    Genevestigatori Q6VVW5.

    Family and domain databases

    Gene3Di 3.30.70.1230. 1 hit.
    InterProi IPR001054. A/G_cyclase.
    IPR018297. A/G_cyclase_CS.
    IPR001828. ANF_lig-bd_rcpt.
    IPR011009. Kinase-like_dom.
    IPR001170. Ntpep_rcpt.
    IPR028082. Peripla_BP_I.
    IPR000719. Prot_kinase_dom.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    [Graphical view ]
    Pfami PF01094. ANF_receptor. 1 hit.
    PF00211. Guanylate_cyc. 1 hit.
    PF07714. Pkinase_Tyr. 1 hit.
    [Graphical view ]
    PRINTSi PR00255. NATPEPTIDER.
    SMARTi SM00044. CYCc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53822. SSF53822. 1 hit.
    SSF55073. SSF55073. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS00458. ANF_RECEPTORS. 1 hit.
    PS00452. GUANYLATE_CYCLASE_1. 1 hit.
    PS50125. GUANYLATE_CYCLASE_2. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Regulation of the guanylyl cyclase-B receptor by alternative splicing."
      Tamura N., Garbers D.L.
      J. Biol. Chem. 278:48880-48889(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING.
      Strain: 129S6/SvEvTac.
      Tissue: Spleen.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: FVB/N.
      Tissue: Liver.
    5. "Critical roles of the guanylyl cyclase B receptor in endochondral ossification and development of female reproductive organs."
      Tamura N., Doolittle L.K., Hammer R.E., Shelton J.M., Richardson J.A., Garbers D.L.
      Proc. Natl. Acad. Sci. U.S.A. 101:17300-17305(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiANPRB_MOUSE
    AccessioniPrimary (citable) accession number: Q6VVW5
    Secondary accession number(s): B1AWI8
    , Q6VVW3, Q6VVW4, Q8CGA9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 26, 2005
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 103 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3