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Q6VVW5 (ANPRB_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Atrial natriuretic peptide receptor 2

EC=4.6.1.2
Alternative name(s):
Atrial natriuretic peptide receptor type B
Short name=ANP-B
Short name=ANPR-B
Short name=NPR-B
Guanylate cyclase B
Short name=GC-B
Gene names
Name:Npr2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1047 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Receptor for the C-type natriuretic peptide NPPC/CNP hormone. Has guanylate cyclase activity upon binding of its ligand. May play a role in the regulation of skeletal growth. Ref.5

Catalytic activity

GTP = 3',5'-cyclic GMP + diphosphate.

Subcellular location

Membrane; Single-pass type I membrane protein By similarity.

Tissue specificity

Widely expressed. Expressed in the columnar proliferating and prehypertrophic chondrocyte layers of the tibia. Ref.5

Post-translational modification

Phosphorylation of the protein kinase-like domain is required for full activation by CNP By similarity.

Disruption phenotype

Mice mainly display dwarfism associated with impairment of endochondral ossification, reduced growth of longitudinal vertebra and limb-bone. Self-clasping and priapism that could be due to neuronal disorder are detected. The development of the female reproductive organ is affected. Ref.5

Sequence similarities

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.

Contains 1 guanylate cyclase domain.

Contains 1 protein kinase domain.

Ontologies

Keywords
   Biological processcGMP biosynthesis
Osteogenesis
   Cellular componentMembrane
   Coding sequence diversityAlternative splicing
   DomainSignal
Transmembrane
Transmembrane helix
   LigandGTP-binding
Nucleotide-binding
   Molecular functionLyase
Receptor
   PTMDisulfide bond
Glycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processbone development

Inferred from mutant phenotype PubMed 624676. Source: MGI

cGMP biosynthetic process

Inferred from direct assay Ref.1. Source: MGI

cellular response to granulocyte macrophage colony-stimulating factor stimulus

Inferred from electronic annotation. Source: Ensembl

intracellular signal transduction

Inferred from electronic annotation. Source: InterPro

negative regulation of meiotic cell cycle

Inferred from mutant phenotype PubMed 20947764. Source: MGI

negative regulation of oocyte maturation

Inferred from mutant phenotype PubMed 20947764. Source: MGI

ossification

Inferred from electronic annotation. Source: UniProtKB-KW

receptor guanylyl cyclase signaling pathway

Inferred from electronic annotation. Source: Ensembl

single organism reproductive process

Inferred from mutant phenotype PubMed 20947764. Source: MGI

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Traceable author statement Ref.1. Source: MGI

   Molecular_functionATP binding

Inferred from electronic annotation. Source: Ensembl

GTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

guanylate cyclase activity

Inferred from direct assay Ref.1. Source: MGI

identical protein binding

Inferred from physical interaction Ref.1. Source: MGI

natriuretic peptide receptor activity

Inferred from direct assay Ref.1. Source: MGI

peptide hormone binding

Inferred from electronic annotation. Source: Ensembl

protein kinase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q6VVW5-1)

Also known as: GC-B1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q6VVW5-2)

Also known as: GC-B2;

The sequence of this isoform differs from the canonical sequence as follows:
     520-544: Missing.
Note: Binds ligand, but no cyclase activation.
Isoform 3 (identifier: Q6VVW5-3)

Also known as: GC-B3;

The sequence of this isoform differs from the canonical sequence as follows:
     407-418: PAAHYSGAEKQI → VMGERTGRRDWS
     419-1047: Missing.
Note: Fail to bind the ligand.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1616 Potential
Chain17 – 10471031Atrial natriuretic peptide receptor 2
PRO_0000012365

Regions

Topological domain17 – 458442Extracellular Potential
Transmembrane459 – 47820Helical; Potential
Topological domain479 – 1047569Cytoplasmic Potential
Domain513 – 786274Protein kinase
Domain861 – 991131Guanylate cyclase

Amino acid modifications

Modified residue5131Phosphoserine By similarity
Modified residue5161Phosphothreonine By similarity
Modified residue5181Phosphoserine By similarity
Modified residue5231Phosphoserine By similarity
Modified residue5261Phosphoserine By similarity
Modified residue5291Phosphothreonine By similarity
Glycosylation241N-linked (GlcNAc...) Potential
Glycosylation351N-linked (GlcNAc...) Potential
Glycosylation1611N-linked (GlcNAc...) Potential
Glycosylation1951N-linked (GlcNAc...) Potential
Glycosylation2441N-linked (GlcNAc...) Potential
Glycosylation2771N-linked (GlcNAc...) Potential
Glycosylation3491N-linked (GlcNAc...) Potential
Disulfide bond75 ↔ 101 By similarity
Disulfide bond439Interchain By similarity
Disulfide bond448Interchain By similarity

Natural variations

Alternative sequence407 – 41812PAAHY…AEKQI → VMGERTGRRDWS in isoform 3.
VSP_013584
Alternative sequence419 – 1047629Missing in isoform 3.
VSP_013585
Alternative sequence520 – 54425Missing in isoform 2.
VSP_013583

Experimental info

Sequence conflict9901M → K in AAQ02634. Ref.1
Sequence conflict9901M → K in AAQ02635. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (GC-B1) [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 34E6C00E561D6D87

FASTA1,047117,060
        10         20         30         40         50         60 
MALPSLLLVV AALAGGVRPP GARNLTLAVV LPEHNLSYAW AWPRVGPAVA LAVEALGRAL 

        70         80         90        100        110        120 
PVDLRFVSSE LDGACSEYLA PLRAVDLKLY HDPDLLLGPG CVYPAASVAR FASHWRLPLL 

       130        140        150        160        170        180 
TAGAVASGFA AKNEHYRTLV RTGPSAPKLG EFVVTLHGHF NWTARAALLY LDARTDDRPH 

       190        200        210        220        230        240 
YFTIEGVFEA LQGSNLSVQH QVYAREPGGP EQATHFIRAN GRIVYICGPL EMLHEILLQA 

       250        260        270        280        290        300 
QRENLTNGDY VFFYLDVFGE SLRAGPTRAT GRPWQDNRTQ EQAQALREAF QTVLVITYRE 

       310        320        330        340        350        360 
PPNPEYQEFQ NRLLIRARED FGVELAPSLM NLIAGCFYDG ILLYAQVLNE TIQEGGTRED 

       370        380        390        400        410        420 
GLRIVEKMQG RRYHGVTGLV VMDKNNDRET DFVLWAMGDL DSGDFQPAAH YSGAEKQIWW 

       430        440        450        460        470        480 
TGRPIPWVKG APPLDNPPCA FDLDDPSCDK TPLSTLAIVA LGTGVTFIMF GVSSFLIFRK 

       490        500        510        520        530        540 
LMLEKELASM LWRIRWEELQ FGNSDRYHKG AGSRLTLSLR GSSYGSLMTA HGKYQIFANT 

       550        560        570        580        590        600 
GHFKGNVVAI KHVNKKRIEL TRQVLFELKH MRDVQFNHLT RFIGACIDPP NICIVTEYCP 

       610        620        630        640        650        660 
RGSLQDILEN DSINLDWMFR YSLINDLVKG MAFLHNSIIS SHGSLKSSNC VVDSRFVLKI 

       670        680        690        700        710        720 
TDYGLASFRS TAEPDDSHAL YAKKLWTAPE LLSGNPLPTT GMQKADVYSF AIILQEIALR 

       730        740        750        760        770        780 
SGPFYLEGLD LSPKEIVQKV RNGQRPYFRP SIDRTQLNEE LVLLMERCWA QDPTERPDFG 

       790        800        810        820        830        840 
QIKGFIRRFN KEGGTSILDN LLLRMEQYAN NLEKLVEERT QAYLEEKRKA EALLYQILPH 

       850        860        870        880        890        900 
SVAEQLKRGE TVQAEAFDSV TIYFSDIVGF TALSAESTPM QVVTLLNDLY TCFDAIIDNF 

       910        920        930        940        950        960 
DVYKVETIGD AYMVVSGLPG RNGQRHAPEI ARMALALLDA VSSFRIRHRP HDQLRLRIGV 

       970        980        990       1000       1010       1020 
HTGPVCAGVV GLKMPRYCLF GDTVNTASRM ESNGQALKIH VSSTTKDALD ELGCFQLELR 

      1030       1040 
GDVEMKGKGK MRTYWLLGEQ KGPPGLL 

« Hide

Isoform 2 (GC-B2) [UniParc].

Checksum: C8CD69A59CDF1C4C
Show »

FASTA1,022114,319
Isoform 3 (GC-B3) [UniParc].

Checksum: 8F6E16CCD6D17C2E
Show »

FASTA41846,443

References

« Hide 'large scale' references
[1]"Regulation of the guanylyl cyclase-B receptor by alternative splicing."
Tamura N., Garbers D.L.
J. Biol. Chem. 278:48880-48889(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: 129S6/SvEvTac.
Tissue: Spleen.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: FVB/N.
Tissue: Liver.
[5]"Critical roles of the guanylyl cyclase B receptor in endochondral ossification and development of female reproductive organs."
Tamura N., Doolittle L.K., Hammer R.E., Shelton J.M., Richardson J.A., Garbers D.L.
Proc. Natl. Acad. Sci. U.S.A. 101:17300-17305(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY323833, AY323832 Genomic DNA. Translation: AAQ02634.1.
AY323833, AY323832 Genomic DNA. Translation: AAQ02635.1.
AY323833, AY323832 Genomic DNA. Translation: AAQ02636.1.
AL732626 Genomic DNA. Translation: CAM17047.1.
CH466565 Genomic DNA. Translation: EDL02456.1.
BC042470 mRNA. Translation: AAH42470.1.
CCDSCCDS18105.1. [Q6VVW5-1]
RefSeqNP_776149.1. NM_173788.3. [Q6VVW5-1]
XP_006537896.1. XM_006537833.1. [Q6VVW5-2]
UniGeneMm.103477.

3D structure databases

ProteinModelPortalQ6VVW5.
SMRQ6VVW5. Positions 24-442, 503-832, 854-1036.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid230934. 2 interactions.
MINTMINT-4997499.

PTM databases

PhosphoSiteQ6VVW5.

Proteomic databases

PaxDbQ6VVW5.
PRIDEQ6VVW5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000030191; ENSMUSP00000030191; ENSMUSG00000028469. [Q6VVW5-1]
GeneID230103.
KEGGmmu:230103.
UCSCuc008sqm.1. mouse. [Q6VVW5-1]

Organism-specific databases

CTD4882.
MGIMGI:97372. Npr2.

Phylogenomic databases

eggNOGCOG2114.
GeneTreeENSGT00710000106571.
HOGENOMHOG000293307.
HOVERGENHBG051862.
InParanoidB1AWI8.
KOK12324.
OMAAAKSEHY.
OrthoDBEOG7Z69BJ.
TreeFamTF106338.

Gene expression databases

ArrayExpressQ6VVW5.
BgeeQ6VVW5.
CleanExMM_NPR2.
GenevestigatorQ6VVW5.

Family and domain databases

Gene3D3.30.70.1230. 1 hit.
InterProIPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR001828. ANF_lig-bd_rcpt.
IPR011009. Kinase-like_dom.
IPR001170. Ntpep_rcpt.
IPR028082. Peripla_BP_I.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view]
PfamPF01094. ANF_receptor. 1 hit.
PF00211. Guanylate_cyc. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSPR00255. NATPEPTIDER.
SMARTSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMSSF53822. SSF53822. 1 hit.
SSF55073. SSF55073. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEPS00458. ANF_RECEPTORS. 1 hit.
PS00452. GUANYLATE_CYCLASE_1. 1 hit.
PS50125. GUANYLATE_CYCLASE_2. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSNPR2. mouse.
NextBio379796.
PROQ6VVW5.
SOURCESearch...

Entry information

Entry nameANPRB_MOUSE
AccessionPrimary (citable) accession number: Q6VVW5
Secondary accession number(s): B1AWI8 expand/collapse secondary AC list , Q6VVW3, Q6VVW4, Q8CGA9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot