UniProtKB - Q6VV64 (KCNKI_MOUSE)
(max 400 entries)x
Your basket is currently empty. i
Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)
Protein
Potassium channel subfamily K member 18
Gene
Kcnk18
Organism
Mus musculus (Mouse)
Status
Functioni
Outward rectifying potassium channel. Produces rapidly activating outward rectifier K+ currents. May function as background potassium channel that sets the resting membrane potential. Channel activity is directly activated by calcium signal. Activated by the G(q)-protein coupled receptor pathway. The calcium signal robustly activates the channel via calcineurin, whereas the anchoring of 14-3-3/YWHAH interferes with the return of the current to the resting state after activation. Inhibited also by arachidonic acid and other naturally occurring unsaturated free fatty acids. Channel activity is also enhanced by volatile anesthetics, such as isoflurane. Appears to be the primary target of hydroxy-alpha-sanshool, an ingredient of Schezuan pepper. May be involved in the somatosensory function with special respect to pain sensation.3 Publications
Miscellaneous
Regulated by extracellular protons whereas human ortholog is not. His-132 is responsible for proton-dependent specific activity.
GO - Molecular functioni
- calcium-activated potassium channel activity Source: UniProtKB
- outward rectifier potassium channel activity Source: MGI
- potassium ion leak channel activity Source: GO_Central
GO - Biological processi
- cellular response to pH Source: MGI
- potassium ion export Source: MGI
- potassium ion transport Source: UniProtKB
- stabilization of membrane potential Source: GO_Central
Keywordsi
Molecular function | Ion channel, Potassium channel |
Biological process | Ion transport, Potassium transport, Transport |
Ligand | Potassium |
Enzyme and pathway databases
Reactomei | R-MMU-1299344. TWIK-related spinal cord K+ channel (TRESK). R-MMU-5576886. Phase 4 - resting membrane potential. |
Protein family/group databases
TCDBi | 1.A.1.18.1. the voltage-gated ion channel (vic) superfamily. |
Names & Taxonomyi
Protein namesi | Recommended name: Potassium channel subfamily K member 18Alternative name(s): Two-pore-domain potassium channel TRESK |
Gene namesi | Name:Kcnk18 Synonyms:Tresk-2, Tresk2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2685627. Kcnk18. |
Subcellular locationi
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 31 | CytoplasmicSequence analysisAdd BLAST | 31 | |
Transmembranei | 32 – 52 | HelicalSequence analysisAdd BLAST | 21 | |
Intramembranei | 114 – 140 | Pore-forming; Name=Pore-forming 1Sequence analysisAdd BLAST | 27 | |
Transmembranei | 142 – 162 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 163 – 292 | CytoplasmicSequence analysisAdd BLAST | 130 | |
Transmembranei | 293 – 313 | HelicalSequence analysisAdd BLAST | 21 | |
Intramembranei | 326 – 340 | Pore-forming; Name=Pore-forming 2Sequence analysisAdd BLAST | 15 | |
Transmembranei | 347 – 367 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 368 – 394 | CytoplasmicSequence analysisAdd BLAST | 27 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 83 | N → Q: Strongly reduced current amplitude and localization to cell membrane. 1 Publication | 1 | |
Mutagenesisi | 132 | H → N: Insensitive to extracellular protons. 1 Publication | 1 | |
Mutagenesisi | 212 | I → A: Loss of interaction with calcineurin and activation by elevated intracellular calcium; when associated with A-214. | 1 | |
Mutagenesisi | 214 | I → A: Strongly reduced activation by elevated intracellular calcium. Loss of interaction with calcineurin and activation by elevated intracellular calcium; when associated with A-212. | 1 | |
Mutagenesisi | 264 | S → A: Loss of interaction with YWHAH. 1 Publication | 1 | |
Mutagenesisi | 276 | S → A: Enhances basal channel activity and abolishes stimulation by calcineurin. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000312501 | 1 – 394 | Potassium channel subfamily K member 18Add BLAST | 394 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 83 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Modified residuei | 264 | Phosphoserine1 Publication | 1 | |
Modified residuei | 276 | Phosphoserine1 Publication | 1 |
Post-translational modificationi
Phosphorylation of Ser-264 is required for the binding of 14-3-3eta/YWHAH. Calcineurin-mediated dephosphorylation of Ser-276 enhances channel activity.2 Publications
N-glycosylated.1 Publication
Keywords - PTMi
Glycoprotein, PhosphoproteinProteomic databases
PaxDbi | Q6VV64. |
PRIDEi | Q6VV64. |
PTM databases
iPTMneti | Q6VV64. |
PhosphoSitePlusi | Q6VV64. |
Expressioni
Tissue specificityi
Detected in brain cortex, cerebellum, dorsal root ganglion, spinal cord and testis. High expression in trigeminal ganglion, also expressed in autonomic nervous system ganglia such as the stellate ganglion and paravertebral sympathetic ganglia. Expressed in all adult spinal cord and brain regions, with slightly higher expression in thalamus, hypothalamus, hippocampus and posterior corte (at protein level). In non-neuronal tissues, substantial expression found in lung and heart and weal expression in liver, testis, kidney, small intestine and spleen.4 Publications
Developmental stagei
Expression appears in trigeminal ganglion and dorsal root ganglia from E15.5 and increased through E18 to reach a peak in newborn mouse postnatal day 1.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000040901. |
CleanExi | MM_KCNK18. |
Interactioni
Subunit structurei
Interacts with calcineurin. Interacts with YWHAH, in a phosphorylation-dependent manner.2 Publications
Protein-protein interaction databases
ELMi | Q6VV64. |
IntActi | Q6VV64. 1 interactor. |
MINTi | Q6VV64. |
STRINGi | 10090.ENSMUSP00000065713. |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 210 – 215 | Interaction with calcineurin | 6 | |
Regioni | 261 – 266 | Interaction with YWHAH1 Publication | 6 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1418. Eukaryota. COG1226. LUCA. |
GeneTreei | ENSGT00700000104522. |
HOGENOMi | HOG000074045. |
HOVERGENi | HBG104673. |
InParanoidi | Q6VV64. |
KOi | K20007. |
OMAi | FKLVQNR. |
OrthoDBi | EOG091G0B4G. |
PhylomeDBi | Q6VV64. |
TreeFami | TF316115. |
Family and domain databases
InterProi | View protein in InterPro IPR003280. 2pore_dom_K_chnl. IPR003092. 2pore_dom_K_chnl_TASK. IPR013099. K_chnl_dom. |
Pfami | View protein in Pfam PF07885. Ion_trans_2. 2 hits. |
PRINTSi | PR01333. 2POREKCHANEL. PR01095. TASKCHANNEL. |
i Sequence
Sequence statusi: Complete.
Q6VV64-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEAEEPPEAR RCCPEALGKA RGCCPEALGK LLPGLCFLCC LVTYALVGAA
60 70 80 90 100
LFSAVEGRPD PEAEENPELK KFLDDLCNIL KCNLTVVEGS RKNLCEHLQH
110 120 130 140 150
LKPQWLKAPQ DWSFLSALFF CCTVFSTVGY GHMYPVTRLG KFLCMLYALF
160 170 180 190 200
GIPLMFLVLT DIGDILATIL SRAYSRFQAL LCLPHDIFKW RSLPLCRKQP
210 220 230 240 250
DSKPVEEAIP QIVIDAGVDE LLNPQPSKDP PSPSCNVELF ERLVAREKKN
260 270 280 290 300
KLQPPTRPVE RSNSCPELVL GRLSCSILSN LDEVGQQVER LDIPLPVIAL
310 320 330 340 350
VVFAYISCAA AILPFWETEL GFEDAFYFCF VTLTTIGFGD IVLVHPHFFL
360 370 380 390
FFSIYIIVGM EILFIAFKLM QNRLLHTYKT LMLFVCQREV SLPW
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 228 | K → R in AAI15706 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 228 | K → R in AAI04133 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 251 | K → E in AAI15706 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 251 | K → E in AAI04133 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY325301 mRNA. Translation: AAQ91836.1. AY542902 mRNA. Translation: AAS48426.1. AK139600 mRNA. Translation: BAE24080.1. AK162136 mRNA. Translation: BAE36746.1. BC104132 mRNA. Translation: AAI04133.1. BC104133 mRNA. Translation: AAI04134.1. BC115705 mRNA. Translation: AAI15706.1. BC115887 mRNA. Translation: AAI15888.1. BC127136 mRNA. Translation: AAI27137.1. BC127137 mRNA. Translation: AAI27138.1. |
CCDSi | CCDS29934.1. |
RefSeqi | NP_997144.1. NM_207261.3. |
UniGenei | Mm.329947. |
Genome annotation databases
Ensembli | ENSMUST00000065204; ENSMUSP00000065713; ENSMUSG00000040901. |
GeneIDi | 332396. |
KEGGi | mmu:332396. |
UCSCi | uc008ibh.1. mouse. |
Similar proteinsi
Entry informationi
Entry namei | KCNKI_MOUSE | |
Accessioni | Q6VV64Primary (citable) accession number: Q6VV64 Secondary accession number(s): Q1LZJ5 Q3MI51 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 4, 2007 |
Last sequence update: | July 5, 2004 | |
Last modified: | February 28, 2018 | |
This is version 119 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |