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Protein

Activating transcription factor 7-interacting protein 1

Gene

ATF7IP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Recruiter that couples transcriptional factors to general transcription apparatus and thereby modulates transcription regulation and chromatin formation. Can both act as an activator or a repressor depending on the context. Mediates MBD1-dependent transcriptional repression, probably by recruiting complexes containing SETDB1. Required to stimulate histone methyltransferase activity of SETDB1 and facilitate the conversion of dimethylated to trimethylated H3 'Lys-9' (H3K9me3). The complex formed with MBD1 and SETDB1 represses transcription and couples DNA methylation and histone H3 'Lys-9' trimethylation (H3K9me3). Facilitates telomerase TERT and TERC gene expression by SP1 in cancer cells.3 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Host-virus interaction, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-3214841. PKMTs methylate histone lysines.

Names & Taxonomyi

Protein namesi
Recommended name:
Activating transcription factor 7-interacting protein 1
Alternative name(s):
ATF-interacting protein
Short name:
ATF-IP
ATF7-interacting protein
ATFa-associated modulator
Short name:
hAM
MBD1-containing chromatin-associated factor 1
P621
Gene namesi
Name:ATF7IP
Synonyms:MCAF, MCAF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:20092. ATF7IP.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: HPA
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
  • transcription factor complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi968D → A: Abolishes the interaction with SUMO. 1 Publication1
Mutagenesisi969L → A: Abolishes the interaction with SUMO. 1 Publication1
Mutagenesisi1224L → R: Abolishes interaction with MBD1 and subsequent transcriptional repression. 1 Publication1

Organism-specific databases

DisGeNETi55729.
OpenTargetsiENSG00000171681.

Polymorphism and mutation databases

BioMutaiATF7IP.
DMDMi317373420.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002817801 – 1270Activating transcription factor 7-interacting protein 1Add BLAST1270

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei57PhosphoserineCombined sources1
Modified residuei113PhosphoserineCombined sources1
Modified residuei118PhosphothreonineCombined sources1
Modified residuei445PhosphoserineCombined sources1
Modified residuei473PhosphoserineCombined sources1
Modified residuei474PhosphoserineCombined sources1
Modified residuei477PhosphoserineCombined sources1
Modified residuei479PhosphoserineBy similarity1
Modified residuei496PhosphoserineCombined sources1
Modified residuei559PhosphoserineCombined sources1
Modified residuei673PhosphoserineCombined sources1
Modified residuei899PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ6VMQ6.
MaxQBiQ6VMQ6.
PaxDbiQ6VMQ6.
PeptideAtlasiQ6VMQ6.
PRIDEiQ6VMQ6.

PTM databases

iPTMnetiQ6VMQ6.
PhosphoSitePlusiQ6VMQ6.

Expressioni

Tissue specificityi

Detected at low levels in breast, lung and stomach; highly up-regulated in the corresponding cancerous tissues (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000171681.
CleanExiHS_ATF7IP.
ExpressionAtlasiQ6VMQ6. baseline and differential.
GenevisibleiQ6VMQ6. HS.

Organism-specific databases

HPAiHPA016578.
HPA023505.

Interactioni

Subunit structurei

Interacts with MBD1; the interaction is enhanced when MBD1 is sumoylated. Probably forms a complex with SETDB1 and MBD1. Interacts with SUMO ubiquitin-like proteins (SUMO1, SUNO2 and SUMO3), with a preference for SUMO2 and SUMO3. Interacts with SP1, ATF7 and ZHX1. Interacts with the general transcription machinery, including ERCC2, ERCC3, GTF2E1, GTF2E2 and POLR2A. Interacts with Epstein-Barr virus BRLF1/Rta protein, leading to promote and regulate host genes in Epstein-Barr virus-infected cells.9 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
DISC1Q9NRI53EBI-928732,EBI-529989

Protein-protein interaction databases

BioGridi120849. 31 interactors.
IntActiQ6VMQ6. 58 interactors.
MINTiMINT-1179935.
STRINGi9606.ENSP00000261168.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2RPQNMR-B938-981[»]
ProteinModelPortaliQ6VMQ6.
SMRiQ6VMQ6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ6VMQ6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1160 – 1270Fibronectin type-IIIPROSITE-ProRule annotationAdd BLAST111

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni562 – 817Interaction with SETDB1Add BLAST256
Regioni965 – 975Interaction with SUMO1 PublicationAdd BLAST11
Regioni1154 – 1270Interaction with MBD1Add BLAST117

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili617 – 665Sequence analysisAdd BLAST49

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi553 – 571Nuclear localization signalBy similarityAdd BLAST19

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi349 – 580Glu-richAdd BLAST232

Sequence similaritiesi

Belongs to the MCAF family.Curated
Contains 1 fibronectin type-III domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IJ5P. Eukaryota.
ENOG410XUWA. LUCA.
GeneTreeiENSGT00530000063707.
HOVERGENiHBG087180.
InParanoidiQ6VMQ6.
OMAiVRQVNPQ.
OrthoDBiEOG091G058O.
PhylomeDBiQ6VMQ6.
TreeFamiTF329427.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR026085. ATF7-int.
IPR031870. ATF7IP_BD.
IPR003961. FN3_dom.
IPR013783. Ig-like_fold.
[Graphical view]
PANTHERiPTHR23210. PTHR23210. 1 hit.
PfamiPF16788. ATF7IP_BD. 1 hit.
PF16794. fn3_4. 1 hit.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 1 hit.
PROSITEiPS50853. FN3. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6VMQ6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDSLEEPQKK VFKARKTMRV SDRQQLEAVY KVKEELLKTD VKLLNGNHEN
60 70 80 90 100
GDLDPTSPLE NMDYIKDKEE VNGIEEICFD PEGSKAEWKE TPCILSVNVK
110 120 130 140 150
NKQDDDLNCE PLSPHNITPE PVSKLPAEPV SGDPAPGDLD AGDPASGVLA
160 170 180 190 200
SGDSTSGDPT SSEPSSSDAA SGDATSGDAP SGDVSPGDAT SGDATADDLS
210 220 230 240 250
SGDPTSSDPI PGEPVPVEPI SGDCAADDIA SSEITSVDLA SGAPASTDPA
260 270 280 290 300
SDDLASGDLS SSELASDDLA TGELASDELT SESTFDRTFE PKSVPVCEPV
310 320 330 340 350
PEIDNIEPSS NKDDDFLEKN GADEKLEQIQ SKDSLDEKNK ADNNIDANEE
360 370 380 390 400
TLETDDTTIC SDRPPENEKK VEEDIITELA LGEDAISSSM EIDQGEKNED
410 420 430 440 450
ETSADLVETI NENVIEDNKS ENILENTDSM ETDEIIPILE KLAPSEDELT
460 470 480 490 500
CFSKTSLLPI DETNPDLEEK MESSFGSPSK QESSESLPKE AFLVLSDEED
510 520 530 540 550
ISGEKDESEV ISQNETCSPA EVESNEKDNK PEEEEQVIHE DDERPSEKNE
560 570 580 590 600
FSRRKRSKSE DMDNVQSKRR RYMEEEYEAE FQVKITAKGD INQKLQKVIQ
610 620 630 640 650
WLLEEKLCAL QCAVFDKTLA ELKTRVEKIE CNKRHKTVLT ELQAKIARLT
660 670 680 690 700
KRFEAAKEDL KKRHEHPPNP PVSPGKTVND VNSNNNMSYR NAGTVRQMLE
710 720 730 740 750
SKRNVSESAP PSFQTPVNTV SSTNLVTPPA VVSSQPKLQT PVTSGSLTAT
760 770 780 790 800
SVLPAPNTAT VVATTQVPSG NPQPTISLQP LPVILHVPVA VSSQPQLLQS
810 820 830 840 850
HPGTLVTNQP SGNVEFISVQ SPPTVSGLTK NPVSLPSLPN PTKPNNVPSV
860 870 880 890 900
PSPSIQRNPT ASAAPLGTTL AVQAVPTAHS IVQATRTSLP TVGPSGLYSP
910 920 930 940 950
STNRGPIQMK IPISAFSTSS AAEQNSNTTP RIENQTNKTI DASVSKKAAD
960 970 980 990 1000
STSQCGKATG SDSSGVIDLT MDDEESGASQ DPKKLNHTPV STMSSSQPVS
1010 1020 1030 1040 1050
RPLQPIQPAP PLQPSGVPTS GPSQTTIHLL PTAPTTVNVT HRPVTQVTTR
1060 1070 1080 1090 1100
LPVPRAPANH QVVYTTLPAP PAQAPLRGTV MQAPAVRQVN PQNSVTVRVP
1110 1120 1130 1140 1150
QTTTYVVNNG LTLGSTGPQL TVHHRPPQVH TEPPRPVHPA PLPEAPQPQR
1160 1170 1180 1190 1200
LPPEAASTSL PQKPHLKLAR VQSQNGIVLS WSVLEVDRSC ATVDSYHLYA
1210 1220 1230 1240 1250
YHEEPSATVP SQWKKIGEVK ALPLPMACTL TQFVSGSKYY FAVRAKDIYG
1260 1270
RFGPFCDPQS TDVISSTQSS
Length:1,270
Mass (Da):136,394
Last modified:January 11, 2011 - v3
Checksum:i96F6E4FBA1D79385
GO
Isoform 2 (identifier: Q6VMQ6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     520-520: Missing.
     1095-1106: VTVRVPQTTTYV → KRFFLYMAPRYM
     1107-1270: Missing.

Note: No experimental confirmation available.
Show »
Length:1,105
Mass (Da):118,654
Checksum:i4B84876A207DDC12
GO
Isoform 3 (identifier: Q6VMQ6-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MHQDQRFRM

Note: No experimental confirmation available. Gene prediction based on EST data.
Show »
Length:1,278
Mass (Da):137,493
Checksum:i3775C6DF7DC731BD
GO
Isoform 4 (identifier: Q6VMQ6-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     520-520: Missing.

Note: No experimental confirmation available. Gene prediction based on EST data.
Show »
Length:1,269
Mass (Da):136,323
Checksum:i220BCFBC8E0AB7A7
GO

Sequence cautioni

The sequence AAH37312 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence AK001001 differs from that shown. Intron retention.Curated
The sequence AK001001 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAA91751 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti457L → V in AAH37312 (PubMed:15489334).Curated1
Sequence conflicti1182S → G in AAQ92978 (PubMed:14536086).Curated1
Sequence conflicti1182S → G in BAA91751 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_031283278E → K.Corresponds to variant rs2231908dbSNPEnsembl.1
Natural variantiVAR_031284348N → I.1 PublicationCorresponds to variant rs2231909dbSNPEnsembl.1
Natural variantiVAR_031285530K → R.3 PublicationsCorresponds to variant rs3213764dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0559121M → MHQDQRFRM in isoform 3. Curated1
Alternative sequenceiVSP_024035520Missing in isoform 2 and isoform 4. 1 Publication1
Alternative sequenceiVSP_0240381095 – 1106VTVRV…TTTYV → KRFFLYMAPRYM in isoform 2. 1 PublicationAdd BLAST12
Alternative sequenceiVSP_0240391107 – 1270Missing in isoform 2. 1 PublicationAdd BLAST164

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY337596 mRNA. Translation: AAQ92978.1.
AF425650 mRNA. Translation: AAO91864.1.
AC007782 Genomic DNA. No translation available.
AC008114 Genomic DNA. No translation available.
AC008814 Genomic DNA. No translation available.
AC124892 Genomic DNA. No translation available.
CH471094 Genomic DNA. Translation: EAW96320.1.
BC037312 mRNA. Translation: AAH37312.1. Sequence problems.
BC063855 mRNA. Translation: AAH63855.1.
AK001001 mRNA. No translation available.
AK001550 mRNA. Translation: BAA91751.1. Different initiation.
AJ242978 mRNA. Translation: CAB45135.1.
CCDSiCCDS66326.1. [Q6VMQ6-4]
CCDS66327.1. [Q6VMQ6-5]
CCDS73449.1. [Q6VMQ6-2]
CCDS8663.1. [Q6VMQ6-1]
RefSeqiNP_001273443.1. NM_001286514.1. [Q6VMQ6-5]
NP_001273444.1. NM_001286515.1. [Q6VMQ6-2]
NP_060649.3. NM_018179.4. [Q6VMQ6-1]
NP_851997.1. NM_181352.1. [Q6VMQ6-4]
XP_006719171.1. XM_006719108.3. [Q6VMQ6-1]
XP_006719172.1. XM_006719109.3. [Q6VMQ6-1]
XP_011519056.1. XM_011520754.2. [Q6VMQ6-1]
XP_011519057.1. XM_011520755.2. [Q6VMQ6-1]
XP_016875127.1. XM_017019638.1. [Q6VMQ6-1]
XP_016875128.1. XM_017019639.1. [Q6VMQ6-5]
UniGeneiHs.504856.
Hs.591151.

Genome annotation databases

EnsembliENST00000261168; ENSP00000261168; ENSG00000171681. [Q6VMQ6-1]
ENST00000536444; ENSP00000445955; ENSG00000171681. [Q6VMQ6-5]
ENST00000540793; ENSP00000444589; ENSG00000171681. [Q6VMQ6-1]
ENST00000543189; ENSP00000443179; ENSG00000171681. [Q6VMQ6-2]
ENST00000544627; ENSP00000440440; ENSG00000171681. [Q6VMQ6-4]
GeneIDi55729.
KEGGihsa:55729.
UCSCiuc001rbv.3. human. [Q6VMQ6-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY337596 mRNA. Translation: AAQ92978.1.
AF425650 mRNA. Translation: AAO91864.1.
AC007782 Genomic DNA. No translation available.
AC008114 Genomic DNA. No translation available.
AC008814 Genomic DNA. No translation available.
AC124892 Genomic DNA. No translation available.
CH471094 Genomic DNA. Translation: EAW96320.1.
BC037312 mRNA. Translation: AAH37312.1. Sequence problems.
BC063855 mRNA. Translation: AAH63855.1.
AK001001 mRNA. No translation available.
AK001550 mRNA. Translation: BAA91751.1. Different initiation.
AJ242978 mRNA. Translation: CAB45135.1.
CCDSiCCDS66326.1. [Q6VMQ6-4]
CCDS66327.1. [Q6VMQ6-5]
CCDS73449.1. [Q6VMQ6-2]
CCDS8663.1. [Q6VMQ6-1]
RefSeqiNP_001273443.1. NM_001286514.1. [Q6VMQ6-5]
NP_001273444.1. NM_001286515.1. [Q6VMQ6-2]
NP_060649.3. NM_018179.4. [Q6VMQ6-1]
NP_851997.1. NM_181352.1. [Q6VMQ6-4]
XP_006719171.1. XM_006719108.3. [Q6VMQ6-1]
XP_006719172.1. XM_006719109.3. [Q6VMQ6-1]
XP_011519056.1. XM_011520754.2. [Q6VMQ6-1]
XP_011519057.1. XM_011520755.2. [Q6VMQ6-1]
XP_016875127.1. XM_017019638.1. [Q6VMQ6-1]
XP_016875128.1. XM_017019639.1. [Q6VMQ6-5]
UniGeneiHs.504856.
Hs.591151.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2RPQNMR-B938-981[»]
ProteinModelPortaliQ6VMQ6.
SMRiQ6VMQ6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120849. 31 interactors.
IntActiQ6VMQ6. 58 interactors.
MINTiMINT-1179935.
STRINGi9606.ENSP00000261168.

PTM databases

iPTMnetiQ6VMQ6.
PhosphoSitePlusiQ6VMQ6.

Polymorphism and mutation databases

BioMutaiATF7IP.
DMDMi317373420.

Proteomic databases

EPDiQ6VMQ6.
MaxQBiQ6VMQ6.
PaxDbiQ6VMQ6.
PeptideAtlasiQ6VMQ6.
PRIDEiQ6VMQ6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000261168; ENSP00000261168; ENSG00000171681. [Q6VMQ6-1]
ENST00000536444; ENSP00000445955; ENSG00000171681. [Q6VMQ6-5]
ENST00000540793; ENSP00000444589; ENSG00000171681. [Q6VMQ6-1]
ENST00000543189; ENSP00000443179; ENSG00000171681. [Q6VMQ6-2]
ENST00000544627; ENSP00000440440; ENSG00000171681. [Q6VMQ6-4]
GeneIDi55729.
KEGGihsa:55729.
UCSCiuc001rbv.3. human. [Q6VMQ6-1]

Organism-specific databases

CTDi55729.
DisGeNETi55729.
GeneCardsiATF7IP.
H-InvDBHIX0010453.
HGNCiHGNC:20092. ATF7IP.
HPAiHPA016578.
HPA023505.
MIMi613644. gene.
neXtProtiNX_Q6VMQ6.
OpenTargetsiENSG00000171681.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IJ5P. Eukaryota.
ENOG410XUWA. LUCA.
GeneTreeiENSGT00530000063707.
HOVERGENiHBG087180.
InParanoidiQ6VMQ6.
OMAiVRQVNPQ.
OrthoDBiEOG091G058O.
PhylomeDBiQ6VMQ6.
TreeFamiTF329427.

Enzyme and pathway databases

ReactomeiR-HSA-3214841. PKMTs methylate histone lysines.

Miscellaneous databases

ChiTaRSiATF7IP. human.
EvolutionaryTraceiQ6VMQ6.
GeneWikiiATF7IP.
GenomeRNAii55729.
PROiQ6VMQ6.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000171681.
CleanExiHS_ATF7IP.
ExpressionAtlasiQ6VMQ6. baseline and differential.
GenevisibleiQ6VMQ6. HS.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR026085. ATF7-int.
IPR031870. ATF7IP_BD.
IPR003961. FN3_dom.
IPR013783. Ig-like_fold.
[Graphical view]
PANTHERiPTHR23210. PTHR23210. 1 hit.
PfamiPF16788. ATF7IP_BD. 1 hit.
PF16794. fn3_4. 1 hit.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 1 hit.
PROSITEiPS50853. FN3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMCAF1_HUMAN
AccessioniPrimary (citable) accession number: Q6VMQ6
Secondary accession number(s): F5GX74
, G3V1U0, Q4G0T9, Q6P3T3, Q86XW5, Q9NVJ9, Q9NWC2, Q9Y4X8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: January 11, 2011
Last modified: November 30, 2016
This is version 129 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.