Reviewed, UniProtKB/Swiss-Prot Q6VMB4 (PMIP_LEUGO)

Names and origin

Protein names

Recommended name:
    Mitochondrial intermediate peptidase
      Short name=MIP
    EC=3.4.24.59
Alternative name(s):
    Octapeptidyl aminopeptidase

Gene names

Name:	OCT1
Synonyms:	MIP

Organism

Leucoagaricus gongylophorus (Leaf-cutting ant fungus)

Taxonomic identifier

79220 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Homobasidiomycetes › Agaricomycetidae › Agaricales › Agaricaceae › Leucoagaricus

Protein attributes

Sequence length	760 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cleaves proteins, imported into the mitochondrion, to their mature size. While most mitochondrial precursor proteins are processed to the mature form in one step by mitochondrial processing peptidase (MPP), the sequential cleavage by MIP of an octapeptide after initial processing by MPP is a required step for a subgroup of nuclear-encoded precursor proteins destined for the matrix or the inner membrane By similarity.
Catalytic activity	Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.
Cofactor	Binds 1 zinc ion By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the peptidase M3 family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 19	19	Mitochondrion Potential
	Chain	20 – 760	741	Mitochondrial intermediate peptidase		PRO_0000343202
Sites
	Active site	544	1	By similarity
	Metal binding	543	1	Zinc; catalytic By similarity
	Metal binding	547	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6VMB4-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 66EDF1623874E4A1	FASTA	760	85,580
	10 20 30 40 50 60 MLARSVRTLV VSPKTVFRFR GCLFEKHVST ASADDRAIVS LFDSPHAAFK YPSTISTGLF 70 80 90 100 110 120 GHSQLSHPNA FISLAEATLV RAQLLTDRIL RARSSRDELL KVVKNLDRLS DMLCSVIDLA 130 140 150 160 170 180 ELIRNAHPDR NWATAGHHVY EQLCEFMNVL NTHVGLYEVL KLVLADASIV KTLSPEAYQT 190 200 210 220 230 240 ALIFWRDFEK SAINLPPEER QKFVSLSSDI LVLGREFLEN ANAPRPPASI KPEHMVGIKD 250 260 270 280 290 300 KGLGVRLQLQ AQFTRRDLLV YPGSLQAQMI MRSAPDEEPR RRMYIAANSS TDQQIXTLER 310 320 330 340 350 360 LLKTRAELAR LVGRSSFAHM TLDDKMAKTP ENVMNFLGAL IGQTRPFARR ALKTLSARKQ 370 380 390 400 410 420 AHHGLSSLPT IQAWDRDFYC PPEPPAPPIP LPPLTLGTIF MGLSRLFKYL YGITLRPTEA 430 440 450 460 470 480 QTGEVWHSDV HKLEVIDEDK GLIGWIYADL FARHGKSSGA AHYTVRCSRR TDLDDDLGDG 490 500 510 520 530 540 GLTGHEELIQ QNLEFEKVKR HKIPNQDGVY QLPLVVLLCE FTRPSVLKGA TVLEWHDVMT 550 560 570 580 590 600 LFHEMGHAML AMVGRTEYQN VSGTRCATDF VELPSILMEH FLSSPVVLSL FDLDGTHSLR 610 620 630 640 650 660 QVGNTHEDPC HSIDTFSQII LASLDQIYHS PAVLDNSTFS TTDELENLTV SKGVIPHVPS 670 680 690 700 710 720 TSFQTQFGHL FGYGATYYSY LFDRAIASRV WKKVFEKDPL KREVGEKYKL EVLRWGGGRD 730 740 750 760 PWKMVSKLLD ASELEKGDAE AMREVGRWRI EDEVGLPGRH

References

[1]

"Evolution of the gene encoding mitochondrial intermediate peptidase and its cosegregation with the A mating-type locus of mushroom fungi." James T.Y., Kuees U., Rehner S.A., Vilgalys R. Fungal Genet. Biol. 41:381-390(2004) [PubMed: 14761798] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SAR 000701-1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY338827 Genomic DNA. Translation: AAR22310.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	M03.006.
Family and domain databases
InterPro	IPR001567. Pept_M3A_M3B. IPR006025. Pept_M_Zn_BS. [Graphical view]
Pfam	PF01432. Peptidase_M3. 1 hit. [Graphical view]
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PMIP_LEUGO

Accession

Primary (citable) accession number: Q6VMB4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 2008
Last sequence update:	July 5, 2004
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)