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Protein

Protein Daple

Gene

Ccdc88c

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Negative regulator of the canonical Wnt signaling pathway, acting downstream of DVL to inhibit CTNNB1/Beta-catenin stabilization (PubMed:14750955). May also activate the JNK signaling pathway (By similarity).By similarity1 Publication

GO - Molecular functioni

  • PDZ domain binding Source: UniProtKB
  • protein self-association Source: UniProtKB

GO - Biological processi

  • protein destabilization Source: UniProtKB
  • protein homooligomerization Source: UniProtKB
  • regulation of protein phosphorylation Source: UniProtKB
  • stress-activated protein kinase signaling cascade Source: UniProtKB
  • Wnt signaling pathway Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Wnt signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Daple
Alternative name(s):
Coiled-coil domain-containing protein 88C
Dvl-associating protein with a high frequency of leucine residues
Gene namesi
Name:Ccdc88c
Synonyms:Daple
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1915589. Ccdc88c.

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi2007 – 20071G → A: Abolishes binding to DVL1. 1 Publication
Mutagenesisi2009 – 20091V → A: Abolishes binding to DVL1. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 20092009Protein DaplePRO_0000284726Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1435 – 14351PhosphoserineBy similarity
Modified residuei1798 – 17981PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ6VGS5.
MaxQBiQ6VGS5.
PaxDbiQ6VGS5.
PRIDEiQ6VGS5.

PTM databases

iPTMnetiQ6VGS5.
PhosphoSiteiQ6VGS5.

Expressioni

Gene expression databases

BgeeiQ6VGS5.
CleanExiMM_CCDC88C.

Interactioni

Subunit structurei

Homooligomer. Interacts with the PDZ domain of DVL1.1 Publication

GO - Molecular functioni

  • PDZ domain binding Source: UniProtKB
  • protein self-association Source: UniProtKB

Protein-protein interaction databases

IntActiQ6VGS5. 1 interaction.
MINTiMINT-1890259.
STRINGi10090.ENSMUSP00000068629.

Structurei

3D structure databases

ProteinModelPortaliQ6VGS5.
SMRiQ6VGS5. Positions 11-162.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2007 – 20093DVL1-binding1 Publication

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili247 – 425179Sequence analysisAdd
BLAST
Coiled coili456 – 1008553Sequence analysisAdd
BLAST
Coiled coili1190 – 1384195Sequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi2006 – 20094PDZ-bindingSequence analysis

Sequence similaritiesi

Belongs to the CCDC88 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4643. Eukaryota.
ENOG410YFRJ. LUCA.
GeneTreeiENSGT00690000101702.
HOGENOMiHOG000112146.
HOVERGENiHBG057867.
InParanoidiQ6VGS5.
OMAiKEQHQSM.
OrthoDBiEOG7N0C3V.
PhylomeDBiQ6VGS5.
TreeFamiTF320231.

Family and domain databases

InterProiIPR027719. Daple.
IPR008636. Hook-related_fam.
[Graphical view]
PANTHERiPTHR18947. PTHR18947. 2 hits.
PTHR18947:SF31. PTHR18947:SF31. 2 hits.
PfamiPF05622. HOOK. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q6VGS5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDVTVSQLVE LFLQSPLVTW VKTFGSFGSG HQDNLTLYMD LVDGIFLNQI
60 70 80 90 100
MLQIDPRPSN QRINKHVNND VNLRIQNLSI LVRNIKTYYQ EVLQQLIVMN
110 120 130 140 150
LPNVLMIGKD PLSGKSMEEI KKVLLLVLGC AVQCERKEEF IERIKQLDIE
160 170 180 190 200
TQAGIVAHIQ EVTHNQENVF DLQWLDLPDV APEELEALSR NMVFHLRRLI
210 220 230 240 250
DERDECTELI VDLTQERDYL QTQQPPSPGK FSSPDSTPSP TSSLSSEDKQ
260 270 280 290 300
HLAVELADTK ARLRRVRQEL EEKTEQLADT RHEVDQLVLE LQKAKQDNIQ
310 320 330 340 350
LAADARSARA YRDELDSLRE KANRVERLEM DLVRCKEKLH DVDFYKARME
360 370 380 390 400
ELREDNIILI ETKAMLEEQL TASRARSDKV HELEKENLQL KSKLHDLELD
410 420 430 440 450
RDADKKQIEK LLEEYMVLEM AQKQSMKESA HLGWELEQLS KNADLSDASR
460 470 480 490 500
KSFVFELNEC ASSRILKLEK ENQSLQSTIQ GLRDTSLALE ESSLKYGELE
510 520 530 540 550
KENQQLSKKI EKLQTQLERE KQSNQDLETL SEELIREKEQ LQSGMEALKA
560 570 580 590 600
DRARQIKDLE QEKGHLHQAV WSLRERPQVN STKDVEKENR ALHQAVTEAG
610 620 630 640 650
SKLSQLELEK QQLHRDLEEA KEKGEQAEAL EKELHRLEKE NEQLTKEVTS
660 670 680 690 700
LKAATEKVEA LEHQSQGLEL ENRSLRKSLD TLQNVSVQLE GLERDKQQLG
710 720 730 740 750
QENLELRKMV EAMRFTSAKM AQIETENRQL EREKEELRRD VELLKTLSKK
760 770 780 790 800
SERLELSYQS VSAENLQLQH SLESSTHKSQ ALQRELSQLE AERQALRRDL
810 820 830 840 850
ETLQLTHKQL EGAEEDRKAL EQEVAQLEKD KKLLEKEARR LWQQVELKDA
860 870 880 890 900
ILDDSAAKLS AAEKESRALD KELARCRDVG SKLKELEKDN RDLTKQVTMH
910 920 930 940 950
TRTLTTLRED LVLEKLKSQQ LSSELDKLSQ ELEKVGLSKD LLLQEDDGHG
960 970 980 990 1000
DGKGKTESAL KTTLAMKEEK IVFLEAQVEE KESLSRQLQI ELQMIKKEHE
1010 1020 1030 1040 1050
QLRQTQEGGD KAQNALKRPP GKVTSHQEKE AWEPSHKEAT MELLRVKDRA
1060 1070 1080 1090 1100
IELERSNAAL QAERQLLKEQ LQHLETQNVS FSSQILTLQK QSAFLQEHTT
1110 1120 1130 1140 1150
TLQTQTAKLQ VENSTLSSQN AALSAQYTVL QSQQAAKEAE HEGLQQQQEQ
1160 1170 1180 1190 1200
LAAVYEALLQ DHKHLGTLYE CQSSEYEALI RQHSCLKTLH RNLELEHKEL
1210 1220 1230 1240 1250
GERHGDLQQR KAELEELEKV LSTEREALER EQKTNAIATS ENQRLRGELD
1260 1270 1280 1290 1300
RISFLHQQLK GEYEELHAHT KELKTSLNNS QLELSRWQVR FDELKEQHQS
1310 1320 1330 1340 1350
MDISLTKMDN HCELLSRLKG NLEEENHHLL SQIQLLSQQN QMLLEQNMES
1360 1370 1380 1390 1400
KEQYHEEQKQ YIDKLNALRR HKEKLEEKIM DQYKFYDPAP KKKNHWIGAK
1410 1420 1430 1440 1450
ALVKLIKPKK EGSRERLKST TDSPPWQLEP SDPASPSPSQ ALRSQTENPD
1460 1470 1480 1490 1500
NPPSGPNCVE ERDTHNGPVG KGPGDLKPKR GSPRGGSVDR TDTSTDPAVK
1510 1520 1530 1540 1550
SWPSEPGSRT FSTSATTAAL SSSTPIPKHL GRTKGCNSDD NLCEPSSEPD
1560 1570 1580 1590 1600
GPYHRQQASR PNSLESSRNA SSNSSPLSLK GSSDHLHSRC ESFSSADLIP
1610 1620 1630 1640 1650
SRDPATLSRD GNTSGRGLLG RHEYPPPRNG PVSQETIQKK GAASTHTGVR
1660 1670 1680 1690 1700
PHSASPSSEM VTLEEFLEES NRGGSPTHDT PSCRDDLLSD YFRKAHDPPA
1710 1720 1730 1740 1750
LGGQPGPPAR KDGAKMPTSF VAPTIKMSIN TSEGQQLKPG HYVKPNLRPS
1760 1770 1780 1790 1800
EAEALAGMPS RQVQPPQSLS LGRPRQTTMT QNCHMPVSRS ASLSRAFSLA
1810 1820 1830 1840 1850
SADLLRASGP EACRPESPQK PGGHEAAGAR ETSTHSLQGS HILARERTPI
1860 1870 1880 1890 1900
VGKADSPSPG QGTRGRPLDT RRFSLAPPKE ERLAPLQQSA TAPALATGCS
1910 1920 1930 1940 1950
SGSNPQIQHF SPTVAPAVRT KSKVPQHSGE VATVAPVRPG LGTSEGDGGP
1960 1970 1980 1990 2000
GHGYSEGLLT KSPGRSSDLP PHVKRGPDDF SQGSSSKSTP ASPEPGGDPQ

TVWYEYGCV
Length:2,009
Mass (Da):226,535
Last modified:July 5, 2004 - v1
Checksum:i68AB26B288393BD4
GO
Isoform 21 Publication (identifier: Q6VGS5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     509-509: K → KAGSLHLQ

Note: No experimental confirmation available.Curated
Show »
Length:2,016
Mass (Da):227,242
Checksum:i069825088B91C1B2
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei509 – 5091K → KAGSLHLQ in isoform 2. 1 PublicationVSP_052377

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY339881 mRNA. Translation: AAR08446.1.
AK137260 mRNA. Translation: BAE23287.1.
CCDSiCCDS26110.1. [Q6VGS5-1]
RefSeqiNP_080957.2. NM_026681.4. [Q6VGS5-1]
UniGeneiMm.156217.

Genome annotation databases

EnsembliENSMUST00000068411; ENSMUSP00000068629; ENSMUSG00000021182. [Q6VGS5-1]
ENSMUST00000085096; ENSMUSP00000082177; ENSMUSG00000021182. [Q6VGS5-2]
GeneIDi68339.
KEGGimmu:68339.
UCSCiuc007ote.1. mouse. [Q6VGS5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY339881 mRNA. Translation: AAR08446.1.
AK137260 mRNA. Translation: BAE23287.1.
CCDSiCCDS26110.1. [Q6VGS5-1]
RefSeqiNP_080957.2. NM_026681.4. [Q6VGS5-1]
UniGeneiMm.156217.

3D structure databases

ProteinModelPortaliQ6VGS5.
SMRiQ6VGS5. Positions 11-162.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ6VGS5. 1 interaction.
MINTiMINT-1890259.
STRINGi10090.ENSMUSP00000068629.

PTM databases

iPTMnetiQ6VGS5.
PhosphoSiteiQ6VGS5.

Proteomic databases

EPDiQ6VGS5.
MaxQBiQ6VGS5.
PaxDbiQ6VGS5.
PRIDEiQ6VGS5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000068411; ENSMUSP00000068629; ENSMUSG00000021182. [Q6VGS5-1]
ENSMUST00000085096; ENSMUSP00000082177; ENSMUSG00000021182. [Q6VGS5-2]
GeneIDi68339.
KEGGimmu:68339.
UCSCiuc007ote.1. mouse. [Q6VGS5-1]

Organism-specific databases

CTDi440193.
MGIiMGI:1915589. Ccdc88c.

Phylogenomic databases

eggNOGiKOG4643. Eukaryota.
ENOG410YFRJ. LUCA.
GeneTreeiENSGT00690000101702.
HOGENOMiHOG000112146.
HOVERGENiHBG057867.
InParanoidiQ6VGS5.
OMAiKEQHQSM.
OrthoDBiEOG7N0C3V.
PhylomeDBiQ6VGS5.
TreeFamiTF320231.

Miscellaneous databases

PROiQ6VGS5.
SOURCEiSearch...

Gene expression databases

BgeeiQ6VGS5.
CleanExiMM_CCDC88C.

Family and domain databases

InterProiIPR027719. Daple.
IPR008636. Hook-related_fam.
[Graphical view]
PANTHERiPTHR18947. PTHR18947. 2 hits.
PTHR18947:SF31. PTHR18947:SF31. 2 hits.
PfamiPF05622. HOOK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of a novel Dvl-binding protein that suppresses Wnt signalling pathway."
    Oshita A., Kishida S., Kobayashi H., Michiue T., Asahara T., Asashima M., Kikuchi A.
    Genes Cells 8:1005-1017(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH DVL1, HOMOOLIGOMERIZATION, MUTAGENESIS OF GLY-2007 AND VAL-2009.
    Tissue: Brain1 Publication.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-966 (ISOFORM 2).
    Strain: C57BL/6JImported.
    Tissue: Urinary bladderImported.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Spleen and Testis.

Entry informationi

Entry nameiDAPLE_MOUSE
AccessioniPrimary (citable) accession number: Q6VGS5
Secondary accession number(s): Q3UVI2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: July 5, 2004
Last modified: June 8, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.