Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

WAS protein family homolog 2

Gene

WASH2P

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Acts as a nucleation-promoting factor at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting. Involved in endocytic trafficking of EGF. Its assembly in the WASH core complex seems to inhibit its NPF activity and via FAM21 is required for its membrane targeting. Involved in transferrin receptor recycling. Regulates the trafficking of endosomal alpha5beta1 integrin to the plasma membrane and involved in invasive cell migration. In T cells involved in endosome-to-membrane recycling of receptors including T-cell receptor (TCR), CD28 and ITGAL; proposed to be implicated in T cell proliferation and effector function. In dendritic cells involved in endosome-to-membrane recycling of major histocompatibility complex (MHC) class II probably involving retromer and subsequently allowing antigen sampling, loading and presentation during T cell activation. Involved in Arp2/3 complex-dependent actin assembly driving Salmonella typhimurium invasion independent of ruffling. Involved in the exocytosis of MMP14 leading to matrix remodeling during invasive migration and implicating late endosome-to-plasma membrane tubular connections and cooperation with the exocyst complex. Involved in negative regulation of autophagy independently from its role in endosomal sorting by inhibiting BECN1 ubiquitination to inactivate PIK3C3/Vps34 activity (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
WAS protein family homolog 2
Alternative name(s):
CXYorf1-like protein on chromosome 2
Protein FAM39B
Gene namesi
Name:WASH2P
Synonyms:FAM39B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Unplaced

Organism-specific databases

HGNCiHGNC:33145. WASH2P.

Subcellular locationi

  • Early endosome membrane By similarity
  • Recycling endosome membrane By similarity
  • Late endosome By similarity
  • Cytoplasmic vesicleautophagosome By similarity
  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosomecentriole By similarity

  • Note: Localization to the endosome membrane is mediated via its interaction with FAM21.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endosome, Membrane

Pathology & Biotechi

Polymorphism and mutation databases

DMDMi284018148.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 465465WAS protein family homolog 2PRO_0000257971Add
BLAST

Proteomic databases

PaxDbiQ6VEQ5.
PRIDEiQ6VEQ5.

Expressioni

Organism-specific databases

HPAiHPA002689.

Interactioni

Subunit structurei

Component of the WASH core complex also known as WASH regulatory complex SHRC composed of WASH (WASH1, WASH2P or WASH3P), FAM21 (FAM21A or FAM21C), KIAA1033/SWIP, KIAA0196/Strumpellin and CCDC53. The WASH core complex associates with the F-actin-capping protein dimer (formed by CAPZA1, CAPZA2 or CAPZA3 and CAPZB) in a transient or substoichiometric manner which was initially described as WASH complex. Interacts (via WHD1 region) with FAM21C; the interaction is direct. Interacts with alpha-tubulin. Interacts with BECN1; WASH1 and AMBRA1 can competetively interact with BECN1. Interacts with BLOC1S2; may associate with the BLOC-1 complex. Interacts with tubulin gamma chain (TUBG1 or TUBG2). Interacts with EXOC1, EXOC4, EXOC8; in MMP14-positive endosomes in breast tumor cells; indicative for an association with the exocyst complex (By similarity).By similarity

Protein-protein interaction databases

IntActiQ6VEQ5. 4 interactions.
MINTiMINT-7008897.
STRINGi9606.ENSP00000352498.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini361 – 38323WH2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 167167WHD1Add
BLAST
Regioni1 – 5454Required for WASH complex assemblyBy similarityAdd
BLAST
Regioni349 – 465117VCABy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi268 – 33063Pro-richAdd
BLAST
Compositional biasi340 – 3478Poly-Ser

Domaini

The VCA (verprolin, cofilin, acidic) domain promotes actin polymerization by the Arp2/3 complex in vitro.By similarity

Sequence similaritiesi

Belongs to the WASH1 family.Curated
Contains 1 WH2 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG86663.
HOGENOMiHOG000007381.
HOVERGENiHBG066686.
InParanoidiQ6VEQ5.
PhylomeDBiQ6VEQ5.

Family and domain databases

InterProiIPR028290. WASH1.
IPR021854. WASH1_WAHD.
IPR003124. WH2_dom.
[Graphical view]
PANTHERiPTHR23331. PTHR23331. 1 hit.
PfamiPF11945. WASH_WAHD. 1 hit.
[Graphical view]
PROSITEiPS51082. WH2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6VEQ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPVRMQHSL AGQTYAVPLI QPDLRREEAV QQMADALQYL QKVSGDIFSR
60 70 80 90 100
ISQQVEQSRS QVQAIGEKVS LAQAKIEKIK GSKKAIKVFS SAKYPAPERL
110 120 130 140 150
QEYGSIFTGA QDPGLQRRPR HRIQSKHRPL DERALQEKLK DFPVCVSTKP
160 170 180 190 200
EPEDDAEEGL GGLPSNISSV SSLLLFNTTE NLYKKYVFLD PLAGAVTKTH
210 220 230 240 250
VMLGAETEEK LFDAPLSISK REQLEQQVPE NYFYVPDLGQ VPEIDVPSYL
260 270 280 290 300
PDLPGIANDL MYIADLGPGI APSAPGTIPE LPTFHTEVAE PLKVDLQDGV
310 320 330 340 350
LTPPPPPPPP PPAPEVLASA PPLPPSTAAP VGQGARQDDS SSSASPSVQG
360 370 380 390 400
APREVVDPSG GRATLLESIR QAGGIGKAKL RSMKERKLEK KKQKEQEQVR
410 420 430 440 450
ATSQGGHLMS DLFNKLVMRR KGISGKGPGA GEGPGGAFAR VSDSIPPLPP
460
PQQPQAEEDE DDWES
Length:465
Mass (Da):50,312
Last modified:January 19, 2010 - v2
Checksum:i54FD1BB405E391DF
GO

Sequence cautioni

The sequence AL078621 differs from that shown. Reason: Erroneous termination at position 25. Translated as Arg.Curated
The sequence AL078621 differs from that shown. Reason: Erroneous termination at position 180. Translated as Glu.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL078621 Genomic DNA. No translation available.
AY341936 mRNA. Translation: AAQ76875.1.
UniGeneiHs.446466.
Hs.459573.
Hs.709408.

Genome annotation databases

UCSCiuc002tkh.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL078621 Genomic DNA. No translation available.
AY341936 mRNA. Translation: AAQ76875.1.
UniGeneiHs.446466.
Hs.459573.
Hs.709408.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ6VEQ5. 4 interactions.
MINTiMINT-7008897.
STRINGi9606.ENSP00000352498.

Polymorphism and mutation databases

DMDMi284018148.

Proteomic databases

PaxDbiQ6VEQ5.
PRIDEiQ6VEQ5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiuc002tkh.3. human.

Organism-specific databases

GeneCardsiGC02P114341.
H-InvDBHIX0000004.
HIX0023492.
HIX0094926.
HIX0195874.
HGNCiHGNC:33145. WASH2P.
HPAiHPA002689.
neXtProtiNX_Q6VEQ5.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG86663.
HOGENOMiHOG000007381.
HOVERGENiHBG066686.
InParanoidiQ6VEQ5.
PhylomeDBiQ6VEQ5.

Miscellaneous databases

PROiQ6VEQ5.

Family and domain databases

InterProiIPR028290. WASH1.
IPR021854. WASH1_WAHD.
IPR003124. WH2_dom.
[Graphical view]
PANTHERiPTHR23331. PTHR23331. 1 hit.
PfamiPF11945. WASH_WAHD. 1 hit.
[Graphical view]
PROSITEiPS51082. WH2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "Diverse fates of paralogs following segmental duplication of telomeric genes."
    Wong A., Vallender E.J., Heretis K., Ilkin Y., Lahn B.T., Lese Martin C., Ledbetter D.H.
    Genomics 84:239-247(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 202-465, GENE DUPLICATION.
  3. Cited for: GENE DUPLICATION.
  4. "Human subtelomeric WASH genes encode a new subclass of the WASP family."
    Linardopoulou E.V., Parghi S.S., Friedman C., Osborn G.E., Parkhurst S.M., Trask B.J.
    PLoS Genet. 3:E237-E237(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE DUPLICATION.

Entry informationi

Entry nameiWASH2_HUMAN
AccessioniPrimary (citable) accession number: Q6VEQ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: January 19, 2010
Last modified: June 24, 2015
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

WASH genes duplicated to multiple chromosomal ends during primate evolution, with highest copy number reached in humans, whose WASH repertoires probably vary extensively among individuals (PubMed:18159949). It is therefore difficult to determine which gene is functional or not. The telomeric region of chromosome 9p is paralogous to the pericentromeric regions of chromosome 9 as well as to 2q. Paralogous regions contain 7 transcriptional units. Duplicated WASH genes are also present in the Xq/Yq pseudoautosomal region, as well as on chromosome 1 and 15. The chromosome 16 copy seems to be a pseudogene.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.