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Protein

Histidine-containing phosphotransfer protein 2

Gene

AHP2

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as two-component phosphorelay mediators between cytokinin sensor histidine kinases and response regulators (B-type ARRs). Plays an important role in propagating cytokinin signal transduction through the multistep His-to-Asp phosphorelay (By similarity). Functions as positive regulator of the cytokinin signaling pathway. May play a regulatory role in salt and drought tolerance during plant development (PubMed:24578505).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cytokinin signaling pathway, Sensory transduction, Two-component regulatory system

Names & Taxonomyi

Protein namesi
Recommended name:
Histidine-containing phosphotransfer protein 2Curated
Alternative name(s):
OsAHP21 Publication
OsHP1Imported
OsHpt31 Publication
Gene namesi
Name:AHP21 Publication
Synonyms:HP1Imported, OHP21 Publication
Ordered Locus Names:Os09g0567400Imported, LOC_Os09g39400Curated
ORF Names:OJ1155_H10.30Imported
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 9

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Dwarf, narrow leaf, small grain and low fertility phenotypes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 149149Histidine-containing phosphotransfer protein 2PRO_0000247615Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei80 – 801PhosphohistidinePROSITE-ProRule annotation

Post-translational modificationi

Two-component system major event consists of a His-to-Asp phosphorelay between a sensor histidine kinase (HK) and a response regulator (RR). In plants, the His-to-Asp phosphorelay involves an additional intermediate named Histidine-containing phosphotransfer protein (HPt). This multistep phosphorelay consists of a His-Asp-His-Asp sequential transfer of a phosphate group between first an His and an Asp of the HK protein, followed by the transfer to a conserved His of the HPt protein and finally the transfer to an Asp in the receiver domain of the RR protein.Curated

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6VAK4.

Expressioni

Tissue specificityi

Widely expressed.2 Publications

Gene expression databases

ExpressionAtlasiQ6VAK4. baseline and differential.
GenevisibleiQ6VAK4. OS.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi39947.LOC_Os09g39400.1.

Structurei

Secondary structure

1
149
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 1918Combined sources
Helixi25 – 3410Combined sources
Helixi42 – 6524Combined sources
Beta strandi67 – 693Combined sources
Helixi72 – 8918Combined sources
Helixi92 – 10615Combined sources
Helixi110 – 14233Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YVIX-ray2.00A/B2-149[»]
2Q4FX-ray2.00A/B2-149[»]
ProteinModelPortaliQ6VAK4.
SMRiQ6VAK4. Positions 2-143.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ6VAK4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 144106HPtPROSITE-ProRule annotationAdd
BLAST

Domaini

Histidine-containing phosphotransfer domain (HPt) contains an active histidine that mediates the phosphotransfer.Curated

Sequence similaritiesi

Contains 1 HPt domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4747. Eukaryota.
COG2198. LUCA.
HOGENOMiHOG000272680.
InParanoidiQ6VAK4.
OMAiSITREYC.
OrthoDBiEOG09360S25.

Family and domain databases

Gene3Di1.20.120.160. 1 hit.
InterProiIPR008207. Sig_transdc_His_kin_Hpt_dom.
[Graphical view]
PfamiPF01627. Hpt. 1 hit.
[Graphical view]
SUPFAMiSSF47226. SSF47226. 1 hit.
PROSITEiPS50894. HPT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6VAK4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAALRDQL TALLSSMFSQ GLVDEQFQQL QMLQDEGGTP GFVSEVVTLF
60 70 80 90 100
CDDADRIINE IATLLEQPVV NFDKVDAYVH QLKGSSASVG AQKVKFTCMQ
110 120 130 140
FRQFCQDKSR DGCLMALAVV RNDFYDLRNK FQTMLQLEQQ IQAYDPKQQ
Length:149
Mass (Da):16,811
Last modified:July 5, 2004 - v1
Checksum:i38CAED8B091225D3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BR000249 Genomic DNA. Translation: FAA00253.1.
AY345241 mRNA. Translation: AAQ24029.1.
AP005558 Genomic DNA. Translation: BAD46253.1.
AP008215 Genomic DNA. Translation: BAF25875.1.
AP014965 Genomic DNA. Translation: BAT09475.1.
CM000146 Genomic DNA. Translation: EEE70257.1.
RefSeqiXP_015611768.1. XM_015756282.1.
UniGeneiOs.4702.

Genome annotation databases

EnsemblPlantsiOS09T0567400-01; OS09T0567400-01; OS09G0567400.
OS09T0567400-02; OS09T0567400-02; OS09G0567400.
GeneIDi4347886.
GrameneiOS09T0567400-01; OS09T0567400-01; OS09G0567400.
OS09T0567400-02; OS09T0567400-02; OS09G0567400.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BR000249 Genomic DNA. Translation: FAA00253.1.
AY345241 mRNA. Translation: AAQ24029.1.
AP005558 Genomic DNA. Translation: BAD46253.1.
AP008215 Genomic DNA. Translation: BAF25875.1.
AP014965 Genomic DNA. Translation: BAT09475.1.
CM000146 Genomic DNA. Translation: EEE70257.1.
RefSeqiXP_015611768.1. XM_015756282.1.
UniGeneiOs.4702.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YVIX-ray2.00A/B2-149[»]
2Q4FX-ray2.00A/B2-149[»]
ProteinModelPortaliQ6VAK4.
SMRiQ6VAK4. Positions 2-143.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os09g39400.1.

Proteomic databases

PaxDbiQ6VAK4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS09T0567400-01; OS09T0567400-01; OS09G0567400.
OS09T0567400-02; OS09T0567400-02; OS09G0567400.
GeneIDi4347886.
GrameneiOS09T0567400-01; OS09T0567400-01; OS09G0567400.
OS09T0567400-02; OS09T0567400-02; OS09G0567400.

Phylogenomic databases

eggNOGiKOG4747. Eukaryota.
COG2198. LUCA.
HOGENOMiHOG000272680.
InParanoidiQ6VAK4.
OMAiSITREYC.
OrthoDBiEOG09360S25.

Miscellaneous databases

EvolutionaryTraceiQ6VAK4.

Gene expression databases

ExpressionAtlasiQ6VAK4. baseline and differential.
GenevisibleiQ6VAK4. OS.

Family and domain databases

Gene3Di1.20.120.160. 1 hit.
InterProiIPR008207. Sig_transdc_His_kin_Hpt_dom.
[Graphical view]
PfamiPF01627. Hpt. 1 hit.
[Graphical view]
SUPFAMiSSF47226. SSF47226. 1 hit.
PROSITEiPS50894. HPT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOHP2_ORYSJ
AccessioniPrimary (citable) accession number: Q6VAK4
Secondary accession number(s): A0A0P0XRE0, Q0IZJ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: July 5, 2004
Last modified: September 7, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Plant silencing simultaneously OHP1 and OHP2 exhibit phenotypes for a deficiency in cytokinin signaling, including dwarfism with reduced internode lengths, enhanced lateral root growth, early leaf senescence, and reduced tiller numbers and fertility.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.