Q6V1Q9 (VP35_EBOZ5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Polymerase cofactor VP35

Gene names

Name:

VP35

Organism

Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus) [Complete proteome]

Taxonomic identifier

128951 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA negative-strand viruses › Mononegavirales › Filoviridae › Ebolavirus › Zaire ebolavirus

Virus host

Epomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
Homo sapiens (Human) [TaxID: 9606]
Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Acts as a polymerase cofactor in the RNA polymerase transcription and replication complex. Prevents establishment of cellular antiviral state by blocking virus-induced phosphorylation and activation of interferon regulatory factor 3 (IRF3), a transcription factor critical for the induction of interferons alpha and beta. The mechanism by which this blockage occurs remains incompletely defined, a hypothesis suggests that VP35 dsRNA-binding activity prevents activation of IRF3 by sequestering dsRNA. Also inhibits the antiviral effect mediated by the interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2/PKR By similarity.
Subunit structure	Homooligomer. Homomultimerizes via the coiled coil domain. Found in a trimeric complex in which VP35 bridges L and the nucleoprotein. May interact with VP40 By similarity.
Subcellular location	Virion. Host cytoplasm By similarity.
Sequence similarities	Belongs to the filoviridae polymerase cofactor VP35 family.

Ontologies

Keywords
Biological process	Host-virus interaction Inhibition of host IFN-mediated response initiation by virus Inhibition of host IRF7 by virus Inhibition of host TBK1-IKBKE-DDX3 complex by virus Inhibition of host innate immune response by virus Interferon antiviral system evasion Transcription Viral RNA replication Viral immunoevasion
Cellular component	Host cytoplasm Virion
Domain	Coiled coil
Ligand	RNA-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	suppression by virus of host IRF7 activity Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host type I interferon production Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	Polymerase cofactor VP35		PRO_0000245078
Regions
	Coiled coil	96 – 116	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6V1Q9 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: B4BEAF5FBB5B171F	FASTA	340	37,363
	10 20 30 40 50 60 MTTRTKGRGH TAATTQNDRM PGPELSGWIS EQLMTGRIPV SDIFCDIENN PGLCYASQMQ 70 80 90 100 110 120 QTKPNPKTRN SQTQTDPICN HSFEEVVQTL ASLATVVQQQ TIASESLEQR ITSLENGLKP 130 140 150 160 170 180 VYDMAKTISS LNRVCAEMVA KYDLLVMTTG RATATAAATE AYWAEHGQPP PGPSLYEESA 190 200 210 220 230 240 IRGKIESRDE TVPQSVREAF NNLDSTTSLT EENFGKPDIS AKDLRNIMYD HLPGFGTAFH 250 260 270 280 290 300 QLVQVICKLG KDSNSLDIIH AEFQASLAEG DSPQCALIQI TKRVPIFQDA APPVIHIRSR 310 320 330 340 GDIPRACQKS LRPVPPSPKI DRGWVCVFQL QDGKTLGLKI

References

[1]	Chain P.S.G., Ichou M.A., Malfatti S.A., Hajjaj A., Vergez L.M., Paragas J., Do L.H., Jahrling P.B., Smith K.L., McCready P.M., Ibrahim M.S. Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY354458 Genomic RNA. Translation: AAQ55046.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002953. Filo_VP35. [Graphical view]
Pfam	PF02097. Filo_VP35. 1 hit. [Graphical view]
PIRSF	PIRSF018326. VP35_FiloV. 1 hit.
PRINTS	PR01240. FILOVP35.
ProtoNet	Search...

Entry information

Entry name

VP35_EBOZ5

Accession

Primary (citable) accession number: Q6V1Q9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
Last sequence update:	July 5, 2004
Last modified:	February 6, 2013
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program