Reviewed, UniProtKB/Swiss-Prot Q6UY70 (L_GTOVV)

Names and origin

Protein names

Recommended name:
    RNA-directed RNA polymerase
    EC=2.7.7.48
Alternative name(s):
    Protein L

Gene names

Name:

L

Organism

Guanarito virus (isolate Human/Venezuela/NH-95551/1990) (GTOV)

Taxonomic identifier

45219 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Arenaviridae › Arenavirus › New world arenaviruses

Virus host

Homo sapiens (Human) [TaxID: 9606]
Zygodontomys brevicauda [TaxID: 157541]

Protein attributes

Sequence length	2198 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	RNA-dependent RNA polymerase which is responsible for replication and transcription of the viral RNA genome. During transcription, synthesizes 4 subgenomic RNAs, and assures their capping by a cap-snatching mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. The 3'-end of subgenomic mRNAs molecules are heterogeneous and not polyadenylated. The replicase function is to direct synthesis of antigenomic and genomic RNA which are encapsidated and non capped. As a consequence of the use of the same enzyme for both transcription and replication, these mechanisms need to be well coordinated. These processes may be regulated by proteins N and Z in a dose-dependent manner By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Homomultimerizes; the oligomeric structure is essential for the polymerase activity. Interacts with the nucleocapsid protein N By similarity. Interacts with protein Z; this interaction inhibits viral transcription and replication By similarity.
Subcellular location	Virion. Host cytoplasm.
Sequence similarities	Belongs to the arenaviridae RNA polymerase family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication
Cellular component	Host cytoplasm Virion
Ligand	Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Gene Ontology (GO)
Biological process	transcription, RNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW viral genome replication Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2198	2198	RNA-directed RNA polymerase		PRO_0000361636
Regions
	Domain	1161 – 1359	199	RdRp catalytic
Natural variations
	Natural variant	536	1	V → E
	Natural variant	1846	1	S → P

Sequences

	Sequence		Length	Mass (Da)
	Q6UY70-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 8ABCDA93A6EE54C4	FASTA	2,198	250,686
	10 20 30 40 50 60 MDEKVFVLKD FIRRQVPDIP ELSYQKEALL SQVEVPMVLT EGFKLLSCLV EIESCRKNSC 70 80 90 100 110 120 ECNFEQKFVD TILSENGVVA PTLPKVIPDG YRFFNKTLIL LETFVRVNPE EFEKKWKTDM 130 140 150 160 170 180 AKLLSLKEDI HRTGITLVPV VDGRGNYNTD LLPDWATERF RWLLIDLLRE SRGAPTMEIE 190 200 210 220 230 240 DQEYHRLIHS LSKTSNQSLG FENIECLKRV HLNYEERLNE QLLKDIVGEV RESKIREELI 250 260 270 280 290 300 KLKTWYREEI YRKGLGNFVQ TDRKSLLQTL VLSSAHSDSL APECPMCCSK ILDLCYQLSM 310 320 330 340 350 360 RIANQTSLEN NFDEPPLPTT QIEKVYLSLL SACNKIKGKK VFNTRRNTLL FLDLIILNFV 370 380 390 400 410 420 AHVYKTQPSE METLKKAGLI IGEMLLLPND RVLDILVARR LLLKKVESCC NWLDRCRHLL 430 440 450 460 470 480 RKEEPVLWDC VSEFTNVPDF ELLLSLAEEL CSEKPVMHYK PPSSLIGDCA HKDLMSMSDG 490 500 510 520 530 540 EFESLFKCLS HISLSLVNSM KTSFSSRLLV NEKDYKRYYG TVRLKECYVQ RFFLRVGLYG 550 560 570 580 590 600 LLFYQKTGEK SRCYSLYLSD KGNLVELGSF YSDPKRFFLP IFSEFVLLAT CAEMLSWLDF 610 620 630 640 650 660 DEKLVDAVTP LLKILVLSIL SSPTKRSQTF LQGLRYFIMA YVNQAHHIQL MSKLAVECKS 670 680 690 700 710 720 ASDVLIQRLS VKIVDMVLSD GSDPDMHMTR KFKFVLNVSY LCHLITKETP DRLTDQIKCF 730 740 750 760 770 780 EKFMEPKLEF GSLIVNPSLN GFLSKEQEDV MIEGVEKFFS KELLTVEDLK RPGVSRELLS 790 800 810 820 830 840 YCVSLFNKGR LRVNGTLGTD PYRPSFTSTA LDLSSNKSVV IPKLNEVGEI VSEYDKQKLV 850 860 870 880 890 900 STCITSMAER FKTKGRYNLD PDTIDFLIMR NLTNLLSARK LDSSKKEELS LLYEHLSEDV 910 920 930 940 950 960 MKAFEEIKYE VEITLSKMRL SRELECGHKK PCTLEGVWAP FNVLKVIRSE TSVHEIRDFD 970 980 990 1000 1010 1020 PDLLGEDVYE KLCVAVYDSP LRPTFFLEKP LDICPLELLL KNLTTKSYED DEFFDCFKYI 1030 1040 1050 1060 1070 1080 LIQAGFDQRL GAYEHKNRSR LGLSEEAFRL KEDVRVSNRQ SNSEAIADRL DKSFFTSAAL 1090 1100 1110 1120 1130 1140 RNLCFYSEES PTEYTCISPN VGNLKFGLSY KEQVGSNREL YVGDLNTKMM TRLVEDFTEA 1150 1160 1170 1180 1190 1200 VANSMNYTCL NSEKEFERAI CDMKMAVNNG DLCCSLDHSK WGPFMSPALF HAFFGALKFK 1210 1220 1230 1240 1250 1260 ISKTGEQVDL GPVLNVLKWH LHKAVEVPIS VAEAYCTGML KRRLGLMSLS CQSVCEEFFH 1270 1280 1290 1300 1310 1320 QKLLLEEGVP SHIMSVLDMG QGILHNSSDL YGLITEQFIN YCLDFLFDVI PVSYTSSDDQ 1330 1340 1350 1360 1370 1380 ITTFKLPTMS SSEDGLDGFD WLELLCFHDF LSSKFNKFVS PKSVSGTFVA EFKSRFFVMG 1390 1400 1410 1420 1430 1440 EETPLLTKFV SAALHNVKCK TPTQLAETID TICDQCVANG VGIEIVTKIS ERVNRLIRYS 1450 1460 1470 1480 1490 1500 GYPQTPFLAV EKQDVKDWTD GSRGYRLQRN IEHYLQGSEQ LEFVRKCAKK VLLKIKKGQV 1510 1520 1530 1540 1550 1560 FEEYLVQLIG KDGDDALKGF LSYAGCESDE IKDVLKYRWL NLSANGDLRL VLRTKLMSTR 1570 1580 1590 1600 1610 1620 RVLEREQIPT LIKTLQSKLS KNFTKGVKKI LAESINKSAF QSSVASGFIG FCKSMGSKCV 1630 1640 1650 1660 1670 1680 RDGSGGFMYI REVLNKQRVC PCEICAQNPG IIFCSDALTL IPEFSRSILW DYFSLVLTNA 1690 1700 1710 1720 1730 1740 CELGEWVFSS VQPPKVPILL NNPNLFWAVK PRGTRLIEDQ LGLGHVLQSV RRSYPKVFEE 1750 1760 1770 1780 1790 1800 HLVPFMNDLQ VSRTTDFTRL RYLDVCVALD MMNENLGIVS HLLKAKDNSI YIVKQSECAV 1810 1820 1830 1840 1850 1860 AHIRQVEYVN QELGLSPQQI CSNFKIQLVF SSMINPLVIT TSVLKSFFWF NEVLNLEDES 1870 1880 1890 1900 1910 1920 QIDVGELTDF TILIKKYNLN RAMMLDDLTM GYVVSTISEP TIHLVSLKRN SNSIVGEQNS 1930 1940 1950 1960 1970 1980 EMLHGEQVED MYSIVLHIQL EHKRHSTKYH LSRTVVYSYT VECETNITDI EKEPSLATVK 1990 2000 2010 2020 2030 2040 NVVLRASGSI EGHQFLDGVN LVASQPIFTG KKVINLSELL ADSEITETYK EGDAVGSILL 2050 2060 2070 2080 2090 2100 NFGTFYEHID DRYAYEIVGP ECSDSPLVLD GGSILADGKK LSSIKVELTG DVILKALGAL 2110 2120 2130 2140 2150 2160 ESEKEVQSLL TGLWPFIRIN NLKVKMAQED FLLMYEMHRE SLLKSLEVFS EWCEFVDFSV 2170 2180 2190 CYSKSLRDLV ISDSSGSLRL KGITCKPINL SNSVTEIE

References

[1]	Bowen M.D., Thurman K., Minor E., Meyer R.F., Malfatti S.A., Do L.H., Smith K.L., McCready P.M., Chain P.S.G. Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"New insights into the evolutionary relationships between arenaviruses provided by comparative analysis of small and large segment sequences." Charrel R.N., Lemasson J.J., Garbutt M., Khelifa R., De Micco P., Feldmann H., de Lamballerie X. Virology 317:191-196(2003) [PubMed: 14698659] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[3]	"Phylogeny of the genus Arenavirus." Charrel R.N., de Lamballerie X., Emonet S. Curr. Opin. Microbiol. 11:362-368(2008) [PubMed: 18602020] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY358024 Genomic RNA. Translation: AAQ55254.1. AY216504 Genomic RNA. Translation: AAP44540.2.
RefSeq	NP_899221.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2943171.
Family and domain databases
InterPro	IPR014386. RNA-dir_pol_arenavirus. IPR007099. RNA-dir_pol_NSvirus. IPR010453. RNA_pol_arenavir. [Graphical view]
Pfam	PF06317. Arena_RNA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF000836. L_ArenaV. 1 hit.
PROSITE	PS50525. RDRP_SSRNA_NEG_SEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_GTOVV

Accession

Primary (citable) accession number: Q6UY70
Secondary accession number(s): Q6XQI7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)