Q6UY67 (VP40_MABVO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Matrix protein VP40

Alternative name(s):
Membrane-associated protein VP40

Gene names

Name:

VP40

Organism

Lake Victoria marburgvirus (strain Ozolin-75) (MARV) (Marburg virus (strain South Africa/Ozolin/1975)) [Complete proteome]

Taxonomic identifier

482820 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA negative-strand viruses › Mononegavirales › Filoviridae › Marburgvirus › Marburg marburgvirus

Virus host

Chlorocebus aethiops (Green monkey) (Cercopithecus aethiops) [TaxID: 9534]
Homo sapiens (Human) [TaxID: 9606]
Rousettus aegyptiacus (Egyptian rousette) (Egyptian fruit bat) [TaxID: 9407]

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Promotes virus assembly and budding by interacting with host proteins of the multivesicular body pathway. May facilitate virus budding by interacting with the nucleocapsid and the plasma membrane. Specific interactions with membrane-associated GP and VP24 during the budding process may also occur. May play a role in genome replication By similarity.
Subunit structure	Interacts with host TSG101 By similarity.
Subcellular location	Virion membrane; Peripheral membrane protein By similarity. Host late endosome membrane; Peripheral membrane protein By similarity. Host cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Host endomembrane system; Peripheral membrane protein By similarity. Note: In virion, localizes on the intravirional side of the membrane. In the host cell, it is found associated with virus-induced membrane proliferation foci and probably also in multivesicular bodies. These VP40-enriched membrane clusters are then redistributed to the plasma membrane where budding takes place By similarity.
Domain	Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. Gag-p19 contains one L domain: a PPXY motif which potentially interacts with the WW domain 3 of NEDD4 E3 ubiquitin ligase By similarity.
Miscellaneous	Most abundant protein in the virion.
Sequence similarities	Belongs to the filoviridae matrix protein VP40 family.

Ontologies

Keywords
Biological process	Host-virus interaction Inhibition of host JAK1 by virus Inhibition of host innate immune response by virus Inhibition of host interferon signaling pathway by virus Viral budding Viral budding via the host ESCRT complexes Viral immunoevasion Virus exit from host cell
Cellular component	Host cell membrane Host endosome Host membrane Membrane Viral matrix protein Virion
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	suppression by virus of host JAK1 activity Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host type I interferon-mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of tyrosine phosphorylation of host STAT protein Inferred from electronic annotation. Source: UniProtKB-KW viral release from host cell Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell endomembrane system Inferred from electronic annotation. Source: UniProtKB-SubCell host cell late endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell host cell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell virion membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	Matrix protein VP40		PRO_0000314999
Regions
	Motif	16 – 19	4	PPXY motif By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6UY67 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 8B4A058CEC44AEDE	FASTA	303	33,790
	10 20 30 40 50 60 MASSSNYNTY MQYLNPPPYA DHGANQLIPA DQLSNQHGIT PNYVGDLNLD DQFKGNVCHA 70 80 90 100 110 120 FTLEAIIDIS AYNERTVKGV PAWLPLGIMS NFEYPLAHTV AALLTGSYTI TQFTHNGQKF 130 140 150 160 170 180 VRVNRLGTGI PAHPLRMLRE GNQAFIQNMV IPRNFSTNQF TYNLTNLVLS VQKLPDDAWR 190 200 210 220 230 240 PSKDKLIGNT MHPAVSIHPN LPPIVLPTVK KQAYRQHKNP NNGPLLAISG ILHQLRVEKV 250 260 270 280 290 300 PEKTSLFRIS LPADMFSVKE GMMKKRGESS PVVYFQAPEN FPLNGFNNRQ VVLAYANPTL SAI

References

[1]	Bowen M.D., Thurman K., Minor E., Ibrahim M.S., Meyer R.F., Malfatti S.A., Do L.H., Smith K.L., McCready P.M., Chain P.S.G. Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY358025 Genomic RNA. Translation: AAQ55257.1.
3D structure databases
HSSP	HSSP built from PDB template 1H2D based on UniProtKB Q05128.
ProteinModelPortal	Q6UY67.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR008986. EV_matrix. [Graphical view]
Pfam	PF07447. VP40. 1 hit. [Graphical view]
PIRSF	PIRSF018327. VP40_FiloV. 1 hit.
SUPFAM	SSF50012. EV_matrix. 1 hit.
ProtoNet	Search...

Entry information

Entry name

VP40_MABVO

Accession

Primary (citable) accession number: Q6UY67

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 5, 2004
Last modified:	January 9, 2013
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program