Q6UY63 (L_MABVO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-directed RNA polymerase L
Short name=Protein L

Alternative name(s):
Large structural protein
Replicase
Transcriptase

Including the following 3 domains:

1. RNA-directed RNA polymerase
   EC=2.7.7.48
2. mRNA (guanine-N(7)-)-methyltransferase
   EC=2.1.1.56
3. mRNA guanylyltransferase
   EC=2.7.7.-

Gene names

Name:

L

Organism

Lake Victoria marburgvirus (strain Ozolin-75) (MARV) (Marburg virus (strain South Africa/Ozolin/1975)) [Complete proteome]

Taxonomic identifier

482820 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA negative-strand viruses › Mononegavirales › Filoviridae › Marburgvirus › Marburg marburgvirus

Virus host

Chlorocebus aethiops (Green monkey) (Cercopithecus aethiops) [TaxID: 9534]
Homo sapiens (Human) [TaxID: 9606]
Rousettus aegyptiacus (Egyptian rousette) (Egyptian fruit bat) [TaxID: 9407]

Protein attributes

Sequence length	2331 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
Subcellular location	Host cytoplasm. Virion By similarity.
Sequence similarities	Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	Viral RNA replication mRNA capping mRNA processing
Cellular component	Host cytoplasm Virion
Ligand	ATP-binding Nucleotide-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological_process	RNA (guanine-N7)-methylation Inferred from electronic annotation. Source: GOC
Cellular_component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2331	2331	RNA-directed RNA polymerase L		PRO_0000314971
Regions
	Domain	628 – 812	185	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	Q6UY63 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: F5145FBD4B5089AA	FASTA	2,331	267,213
	10 20 30 40 50 60 MQHPTQYPDA RLSSPIVLDQ CDLLARSLGL YSHYSHNPKL RNCRIPHHIY RLRNSTALKT 70 80 90 100 110 120 FLQNCSILTV PFHSIWDHIL TSIQYDAINH VDDFKYLLPS ELVKYANWDN EFLKTYLNKI 130 140 150 160 170 180 LRLDHVFPAS ARSQCEDFSP KENPYYWGML LLVHLSQLAR RIKGQRGSLR SNWKFIGTDL 190 200 210 220 230 240 ELFGIADFII FKVPVKTIIR NAVSLQASKP GLRVWYRDQN LTPYLCDDEF IVSVASYECF 250 260 270 280 290 300 IMIKDVFIER YNTWEICARA WLEDSDGADY PPLDVLGELY NYGDQIIAMY LEDGFKLIKH 310 320 330 340 350 360 LEPLCVSCIQ THGIFTPRKY WFQSQMIKSY YDELCDLNLK LRISDNKAEC AQNFIKTIIQ 370 380 390 400 410 420 ARLTPQQYCE LFSLQKHWGH PVLYNDVALD KVKKHAQATK ILKPKVMFET FCVFKFIVAK 430 440 450 460 470 480 NHYHSQGSWY KTTHDLHLTP YLRQHIVSNS FPSQAEIYQH LWEWYFVEHE PLFSTKIISD 490 500 510 520 530 540 LSIFIKDRAT AVNQECWDSV FDRSVLGYNP PVRFQSKRVP EQFLGQADFS LNQILDFAEK 550 560 570 580 590 600 LEYLAPSYRN FSFSLKEKEL NIGRTFGKLP YRVRNVQTLA EALLADGLAK AFPSNMMVVT 610 620 630 640 650 660 EREQKEALLH QASWHHNSAS IGENAIVRGA SFVTDLEKYN LAFRYEFTRH FINYCNRCYG 670 680 690 700 710 720 VKNLFDWMHF LIPLCYMHVS DFYSPPHCVT EDNRNNPPDC ANAYHYHLGG IEGLQQKLWT 730 740 750 760 770 780 CISCAQITLV ELKTKLKLKS SVMGDNQCIT TLSLFPIDAP NDYQENEAEL NAARVAVELA 790 800 810 820 830 840 ITTGYSGIFL KPEETFVHSG FIYFGKKQYL NGVQLPQSLK TMARCGPLSD SIFDDLQGSL 850 860 870 880 890 900 ASIGTSFERG TSETRHIFPS RWIASFHSML AINLLNQNHL GFPLGFNIDI SCFKKPLTFS 910 920 930 940 950 960 EKLIALITPQ VLGGLSFLNP EKLFYRNISD PLTSGLFQLK NALEFLGKGE LFYILIAKKP 970 980 990 1000 1010 1020 GLADASDFVM NPLGLNVPGS REIITFLRQT VRENITITSQ NRIINSLFHI GSDLEDQKVC 1030 1040 1050 1060 1070 1080 EWLLSSNPVM SRFAADIFSR TPSGKRLQVL GYLEGTRTLL ASRTISLTTE GTMLMKLREL 1090 1100 1110 1120 1130 1140 TRNRWKSWFS YIDALDDDLS ESLEKFTCTV DVANFLRAYS WSDVLKGKRL IGATLPCLLE 1150 1160 1170 1180 1190 1200 QFKVKWINLS EDLREQFNLS SESELTINLL PYDCKELRLG GSHDTELNYV SCALDRKVVQ 1210 1220 1230 1240 1250 1260 KHPSVKRLAW TIGNRAPYIG SRTEDKIGYP PLRVNCPSAA LKEAIEMVSR LLWVTQGTAD 1270 1280 1290 1300 1310 1320 REKLLIPLLN SRVNLDYQTV LNFLPTHYSG NIVHRYNDQY GQHSFMANRM SNTSTRAIIS 1330 1340 1350 1360 1370 1380 TNTLGKYAGG GQAAIDSNII FQNTINLGVA VLDIALSLAK LSSASNVTFR LMLNKCCTRH 1390 1400 1410 1420 1430 1440 VPSEYLFFDK PLDVDLNKYM DNELVYDNDP LCSGIKGRLG RVSRSTLSLS LNVSDIGSYD 1450 1460 1470 1480 1490 1500 FPTIAAWTLG ETIVGSIFSD ESSQSTDPIS SGCTKTFVTH FLVYPVESIF YAFGANLIVE 1510 1520 1530 1540 1550 1560 SLSLSRIKSI KNLSDLTFLI SSTIRNLSHR SLRILQSTFR HELVLTRLAH HIPLISLMLG 1570 1580 1590 1600 1610 1620 GSAGEKSSSD AVRLFLTASY QNFIHNFSCL MKKGQSSLPV WLYFPSEGQQ LKPILKILQR 1630 1640 1650 1660 1670 1680 LSDLLSPDKV QRCKTLADTC CSIDSFWVYP SKSTRTNHYY ASLNYWRDKA NKVKNTPFSH 1690 1700 1710 1720 1730 1740 LINCSFLELS SHTSSVSSNQ QVTNSKYIVH PEDIPEINTR TKLIEYGSTA LQRMDIKMPP 1750 1760 1770 1780 1790 1800 SEQNLVENCR PSKDIRFKDN QKITKHDQRY EKKESSQQQM SPEDNMQTLA YMHNSSPSQT 1810 1820 1830 1840 1850 1860 FIKSIDVHED FDASRVILNS KINNFNLTDC TINTNLLTTP TGTEFLDTSP LQSSRYSSTP 1870 1880 1890 1900 1910 1920 RERSLLSREQ ASYLYVDCSN IPSISLDPGF RNMSDQNQIQ MLINAYKRDL HACFDSNQFC 1930 1940 1950 1960 1970 1980 RFTGVVSSMH YKLYDLLPPG ELRKAICLAE GEGSGARLLL KWKETDHLFF NTLATDSQQE 1990 2000 2010 2020 2030 2040 AEILSGRVIP RMLYNIDRLS ALLESRRLIL NNLTIQITDI TNPLWLDSVI QYLPEDSDIL 2050 2060 2070 2080 2090 2100 TMDAETTKDE TREQLYKTIV NIWTRTSPNI PKISIIKVFL LDYEGTLFLM KNAIQYYGQV 2110 2120 2130 2140 2150 2160 QLKKPYSSNA KNSEWYLCCS KRRIQRLQID FPDQVGIFLI CKAMSRQRQA IPYWLKHIEK 2170 2180 2190 2200 2210 2220 NYPASLHEFF LTLGFPSLES SFCHRYTIPF SEGKALFHKV QSYVRQGKQH LHSLMLDYEN 2230 2240 2250 2260 2270 2280 NSPLLDLRNH FICSLRGKIT KYYNDILKLN LVIKAVEKGK NWSQLVETLP NMHSVCIVHV 2290 2300 2310 2320 2330 DHECFGCEKR LLLKLDFIRN TKIAEQKLLN RVIGYILFFP FGLSKSRSLR A

References

[1]	Bowen M.D., Thurman K., Minor E., Ibrahim M.S., Meyer R.F., Malfatti S.A., Do L.H., Smith K.L., McCready P.M., Chain P.S.G. Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY358025 Genomic RNA. Translation: AAQ55261.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR026890. Mononeg_mRNAcap. IPR014023. Mononeg_RNA_pol_cat. IPR017235. RNA-dir_pol_L_filovirus. IPR025786. RNA-direct_RNA_pol_L. [Graphical view]
Pfam	PF14318. Mononeg_mRNAcap. 1 hit. PF00946. Mononeg_RNA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF037548. RNA_pol_Filoviridae. 1 hit.
TIGRFAMs	TIGR04198. paramyx_RNAcap. 1 hit.
PROSITE	PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_MABVO

Accession

Primary (citable) accession number: Q6UY63

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program