Reviewed, UniProtKB/Swiss-Prot Q6UY61 (L_SABVB)

Names and origin

Protein names

Recommended name:
    RNA-directed RNA polymerase
    EC=2.7.7.48
Alternative name(s):
    Protein L

Gene names

Name:

L

Organism

Sabia virus (isolate Human/Brasil/SPH114202/1990) (SABV)

Taxonomic identifier

45709 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Arenaviridae › Arenavirus › New world arenaviruses

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	2212 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	RNA-dependent RNA polymerase which is responsible for replication and transcription of the viral RNA genome. During transcription, synthesizes 4 subgenomic RNAs, and assures their capping by a cap-snatching mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. The 3'-end of subgenomic mRNAs molecules are heterogeneous and not polyadenylated. The replicase function is to direct synthesis of antigenomic and genomic RNA which are encapsidated and non capped. As a consequence of the use of the same enzyme for both transcription and replication, these mechanisms need to be well coordinated. These processes may be regulated by proteins N and Z in a dose-dependent manner By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Homomultimerizes; the oligomeric structure is essential for the polymerase activity. Interacts with the nucleocapsid protein N By similarity. Interacts with protein Z; this interaction inhibits viral transcription and replication By similarity.
Subcellular location	Virion. Host cytoplasm.
Sequence similarities	Belongs to the arenaviridae RNA polymerase family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication
Cellular component	Host cytoplasm Virion
Ligand	Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Gene Ontology (GO)
Biological process	transcription, RNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW viral genome replication Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2212	2212	RNA-directed RNA polymerase		PRO_0000361646
Regions
	Domain	1175 – 1371	197	RdRp catalytic
Natural variations
	Natural variant	965	1	T → A

Sequences

	Sequence		Length	Mass (Da)
	Q6UY61-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: CB68C2AC3F6A9256	FASTA	2,212	252,797
	10 20 30 40 50 60 MQDPLLGTLS ELKDLVRKTI PDVIELAYQK DALLSQVHPR SVLIEGFKLL SLLVELESCK 70 80 90 100 110 120 VNACHHNYEQ KFIDVILSDG GILCPTLPKV VPDGYNLMGK TLILLETFVR VNPDDFEKKW 130 140 150 160 170 180 KADMSKLISL KTDLGKIGVT LVPVVDGRSN YNTSFVSDWT TERLRWLLIE VLKGMKTTSE 190 200 210 220 230 240 LEIEEQEYHR LIHSLAKTNN QSLGFENLEC LKRNMLSYDQ LLDSSLLVGV KNDVKESKVM 250 260 270 280 290 300 EELIRLKIWY KSEVYEKGLG KFVKTDKKVL LSQLITLGSH EENDSLDCAF CSSRILELCF 310 320 330 340 350 360 KLSVKMHEDV LTRGLNLDGT KTLHSSVQSY LNVLSMCNKI KGSKIFNTRR NTLLFLDLIM 370 380 390 400 410 420 LNFVVDEMVK DSTVIRNLKN AGLIVGQMIL LVNDRVLDIL TANKLIRQKL TTNEKWLSIC 430 440 450 460 470 480 SSVLKRYDLE LWEKLCYLIR VPDFNELFQL AKELVSDRPM MRYSVHKAEE RQCCHKAMEN 490 500 510 520 530 540 FTDDDFKIML KALSHLSLGL INSMKTSFSS RLLINERDYS RYFGNVRLRE CYIQRFPITN 550 560 570 580 590 600 NIIGLLFYQK TGERSRCYSL YIAENGELTE IGSFYCDPKR YFVPIFSEAV ITSMCEEMIN 610 620 630 640 650 660 WLNFDSELVR IVSTQLKTLM LLLLCSPSKR NQTFLQGLRY FIMAYVNQAH HIDLMSKLAV 670 680 690 700 710 720 ECKSSSEIQL QRLCVRLFVS ILSGDNEIEY GFTRRFKFLL NISYLCHFIT KETPDRLTDQ 730 740 750 760 770 780 IKCFEKFLEP KLKFNSVIVN PSLNGTLTES QEHQMISSID RFFSKELLDQ SDVKEPGVSR 790 800 810 820 830 840 ELLGYCVSLF NRGKLRVSGD LKVDPFRPTF TSTALDISSN KSVVVPKLDE LGNIVDKYNK 850 860 870 880 890 900 QLMVSSCVTS LVEMFKTKGR YNLDPDSIDF LVLKNLTNLV SANVPQEKSQ EELSTLYEAL 910 920 930 940 950 960 TEDQISAFEQ VRDEVQLALH KMKSSDAREE RLQDPKRNEK NASKGKILES LWSPHQVNRA 970 980 990 1000 1010 1020 IKNETSIHEI KDFDPDILDS HLVEKLCHEV YNSSQKSLFF LDEPLKSVPL EMLLINLTTI 1030 1040 1050 1060 1070 1080 AYEEEEFFEC FKYLLIQGDF DQKLGTYEHK SRSRLGLSSE ALKVQENARV STRESNAEAI 1090 1100 1110 1120 1130 1140 AKKLDRTFFT SAALRNLCFY SEDSPTEFTS VSTNTGNLKF GLSYKEQVGS NRELYVGDLN 1150 1160 1170 1180 1190 1200 TKLMTRLVED FSEVVTGSMR FSCLNSEKEF ERAICDMKMA VNNGDFSLSM DHSKWGPHMS 1210 1220 1230 1240 1250 1260 PALFFTFLAN LNLTEPKSRT RLNLDPLLNI LKWHLHKTVE VPFNVAQAYC IGKLKRSLGL 1270 1280 1290 1300 1310 1320 MECQCSSLTE EFYHSYLQIQ DEIPSHIMSV LDMGQGILHN LSDLYALITE QFLNYVIHKL 1330 1340 1350 1360 1370 1380 FDIDVTSYTS SDDQISIMKL PLSTKENDED FDWLEIICFH EYLSSKLNKF VSPKSVVGNF 1390 1400 1410 1420 1430 1440 VAEFKSRFFV MGEETPLLTK FVAAALHNVK CKTPTQLAET IDTICDQCVA NGVGVDIVSR 1450 1460 1470 1480 1490 1500 ISERVNRLIS YSGYKETPFL TIVNQDVKDW TDGSRGYRLQ RNIENSFGNQ ELLRLIRRGA 1510 1520 1530 1540 1550 1560 RKVFLEIKKG HVFEENLIGL IGRGGDEALR GFLLYAGFAE NDIVEALRHK WLNPSTFGDL 1570 1580 1590 1600 1610 1620 RLVLRTKIMS SKRILERESV PSLIKTLQSR MSKNFIKGAK KILAESINKS AFQSSVASGF 1630 1640 1650 1660 1670 1680 IGFCKSMGSK CVRDGKGGFM YLKELYNNVN KCGCCICLEW PGVVYCQDSL AKISQFARSI 1690 1700 1710 1720 1730 1740 LWDYFTLVLT NACEIGEWVF SDVKSPSAPP ILSNPNLFWA VKPKIQKHIE DRLSLNHILH 1750 1760 1770 1780 1790 1800 SIKRNYPYLF EEHLAPFMSD LQFNQMMNPS HVKFLDVCIA LDMMNENLGI IGHLLRGRNH 1810 1820 1830 1840 1850 1860 FIYIVKQSEC ASAHIRQSDY VDHELGLSPQ QVCYNFKVQF LFSSMIDPLI VSTSTLKTFF 1870 1880 1890 1900 1910 1920 WFNEVLSIEE EDQIDLGELT DFTLFIKTGH LNRAMTADDI TMGYVCSNLA EEIITLNSYG 1930 1940 1950 1960 1970 1980 SFQEFRSNHP SKNDLSDILK TLTSESIKLT LDIQIVHMRN STKYNISRKI VYTLKALCAL 1990 2000 2010 2020 2030 2040 PLEDCFTKDP VALVESLELF ASGVNGGHLQ LDGVTMVSVL PLLRGKKAVN LAQILMDNDL 2050 2060 2070 2080 2090 2100 AATNDHNVME SVTLDFTKFH DELGDKFCYS LVGPEDQGNP IVLHNGMFMI DNQKLSYLKV 2110 2120 2130 2140 2150 2160 EIFGDTIIKA LGALDSPREI GSLLHGLWPY LKATKQIINF DQTDFEMIYD LHRVVLLESI 2170 2180 2190 2200 2210 AQFGDWVEFA SFKVAFSKHY KDIVVADNLG NLRLKGVTCR LFRQQQSVED IE

References

[1]	Bowen M.D., Thurman K., Minor E., Meyer R.F., Malfatti S.A., Do L.H., Smith K.L., McCready P.M., Chain P.S.G. Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"New insights into the evolutionary relationships between arenaviruses provided by comparative analysis of small and large segment sequences." Charrel R.N., Lemasson J.J., Garbutt M., Khelifa R., De Micco P., Feldmann H., de Lamballerie X. Virology 317:191-196(2003) [PubMed: 14698659] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[3]	"Phylogeny of the genus Arenavirus." Charrel R.N., de Lamballerie X., Emonet S. Curr. Opin. Microbiol. 11:362-368(2008) [PubMed: 18602020] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY358026 Genomic RNA. Translation: AAQ55263.1. AY216506 Genomic RNA. Translation: AAP44542.2.
RefSeq	YP_089660.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3077250.
Family and domain databases
InterPro	IPR014386. RNA-dir_pol_arenavirus. IPR007099. RNA-dir_pol_NSvirus. IPR010453. RNA_pol_arenavir. [Graphical view]
Pfam	PF06317. Arena_RNA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF000836. L_ArenaV. 1 hit.
PROSITE	PS50525. RDRP_SSRNA_NEG_SEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_SABVB

Accession

Primary (citable) accession number: Q6UY61
Secondary accession number(s): Q6XQI5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)