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Protein

Peptidase inhibitor 16

Gene

PI16

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Putative serine protease inhibitor.By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidase inhibitor 16
Short name:
PI-16
Alternative name(s):
Cysteine-rich secretory protein 9
Short name:
CRISP-9
PSP94-binding protein
Gene namesi
Name:PI16
Synonyms:CRISP9, PSPBP
ORF Names:PSEC0164, UNQ289/PRO328
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:21245. PI16.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini28 – 442415ExtracellularSequence analysisAdd
BLAST
Transmembranei443 – 46220HelicalSequence analysisAdd
BLAST
Topological domaini463 – 4631CytoplasmicSequence analysis

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134867616.

Polymorphism and mutation databases

BioMutaiPI16.
DMDMi74749419.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 27271 PublicationAdd
BLAST
Chaini28 – 463436Peptidase inhibitor 16PRO_0000287633Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi114 – 1141N-linked (GlcNAc...)Sequence analysis
Glycosylationi403 – 4031N-linked (GlcNAc...)Sequence analysis
Glycosylationi409 – 4091N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.2 Publications

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ6UXB8.
MaxQBiQ6UXB8.
PaxDbiQ6UXB8.
PeptideAtlasiQ6UXB8.
PRIDEiQ6UXB8.

PTM databases

iPTMnetiQ6UXB8.
PhosphoSiteiQ6UXB8.

Expressioni

Tissue specificityi

Expressed in prostate, testis, ovary and intestine. Concentrates in prostate cancer patient's sera.2 Publications

Gene expression databases

BgeeiQ6UXB8.
CleanExiHS_PI16.
GenevisibleiQ6UXB8. HS.

Organism-specific databases

HPAiHPA043763.

Interactioni

Subunit structurei

Interacts with PSP94/MSMB.1 Publication

Protein-protein interaction databases

STRINGi9606.ENSP00000362778.

Structurei

3D structure databases

ProteinModelPortaliQ6UXB8.
SMRiQ6UXB8. Positions 37-197.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 165129SCPAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni386 – 39510O-glycosylated at one site

Sequence similaritiesi

Belongs to the CRISP family.Curated
Contains 1 SCP domain.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3017. Eukaryota.
COG2340. LUCA.
GeneTreeiENSGT00760000119004.
HOGENOMiHOG000115539.
HOVERGENiHBG104077.
InParanoidiQ6UXB8.
OMAiEGTPCSQ.
OrthoDBiEOG7F7WB4.
PhylomeDBiQ6UXB8.
TreeFamiTF316148.

Family and domain databases

Gene3Di3.40.33.10. 1 hit.
InterProiIPR001283. Allrgn_V5/Tpx1.
IPR018244. Allrgn_V5/Tpx1_CS.
IPR014044. CAP_domain.
[Graphical view]
PANTHERiPTHR10334. PTHR10334. 1 hit.
PfamiPF00188. CAP. 1 hit.
[Graphical view]
PRINTSiPR00837. V5TPXLIKE.
SMARTiSM00198. SCP. 1 hit.
[Graphical view]
SUPFAMiSSF55797. SSF55797. 1 hit.
PROSITEiPS01009. CRISP_1. 1 hit.
PS01010. CRISP_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6UXB8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MHGSCSFLML LLPLLLLLVA TTGPVGALTD EEKRLMVELH NLYRAQVSPT
60 70 80 90 100
ASDMLHMRWD EELAAFAKAY ARQCVWGHNK ERGRRGENLF AITDEGMDVP
110 120 130 140 150
LAMEEWHHER EHYNLSAATC SPGQMCGHYT QVVWAKTERI GCGSHFCEKL
160 170 180 190 200
QGVEETNIEL LVCNYEPPGN VKGKRPYQEG TPCSQCPSGY HCKNSLCEPI
210 220 230 240 250
GSPEDAQDLP YLVTEAPSFR ATEASDSRKM GTPSSLATGI PAFLVTEVSG
260 270 280 290 300
SLATKALPAV ETQAPTSLAT KDPPSMATEA PPCVTTEVPS ILAAHSLPSL
310 320 330 340 350
DEEPVTFPKS THVPIPKSAD KVTDKTKVPS RSPENSLDPK MSLTGARELL
360 370 380 390 400
PHAQEEAEAE AELPPSSEVL ASVFPAQDKP GELQATLDHT GHTSSKSLPN
410 420 430 440 450
FPNTSATANA TGGRALALQS SLPGAEGPDK PSVVSGLNSG PGHVWGPLLG
460
LLLLPPLVLA GIF
Length:463
Mass (Da):49,471
Last modified:July 5, 2004 - v1
Checksum:i3B9E7565364CF0D2
GO
Isoform 2 (identifier: Q6UXB8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     231-423: Missing.

Note: No experimental confirmation available.
Show »
Length:270
Mass (Da):29,674
Checksum:i04C0F6769E59B5C3
GO

Sequence cautioni

The sequence AAH22399.2 differs from that shown. Reason: Frameshift at position 400. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti179 – 1791E → G in BAC11640 (PubMed:16303743).Curated
Sequence conflicti288 – 2881V → A in BAC11640 (PubMed:16303743).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti50 – 501T → P.3 Publications
Corresponds to variant rs1405069 [ dbSNP | Ensembl ].
VAR_032337
Natural varianti416 – 4161L → V.
Corresponds to variant rs16889318 [ dbSNP | Ensembl ].
VAR_032338

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei231 – 423193Missing in isoform 2. 1 PublicationVSP_025574Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY358422 mRNA. Translation: AAQ88788.1.
AK075470 mRNA. Translation: BAC11640.1.
AK124589 mRNA. Translation: BAC85892.1.
AL122034 Genomic DNA. Translation: CAI23302.1.
BC022399 mRNA. Translation: AAH22399.2. Frameshift.
BC035634 mRNA. Translation: AAH35634.2.
CCDSiCCDS34440.1. [Q6UXB8-1]
RefSeqiNP_001186088.1. NM_001199159.1. [Q6UXB8-1]
NP_699201.2. NM_153370.2. [Q6UXB8-1]
XP_005248974.1. XM_005248917.1. [Q6UXB8-2]
XP_011512677.1. XM_011514375.1. [Q6UXB8-1]
UniGeneiHs.25391.

Genome annotation databases

EnsembliENST00000373674; ENSP00000362778; ENSG00000164530. [Q6UXB8-1]
ENST00000611814; ENSP00000478888; ENSG00000164530. [Q6UXB8-1]
GeneIDi221476.
KEGGihsa:221476.
UCSCiuc003ona.3. human. [Q6UXB8-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY358422 mRNA. Translation: AAQ88788.1.
AK075470 mRNA. Translation: BAC11640.1.
AK124589 mRNA. Translation: BAC85892.1.
AL122034 Genomic DNA. Translation: CAI23302.1.
BC022399 mRNA. Translation: AAH22399.2. Frameshift.
BC035634 mRNA. Translation: AAH35634.2.
CCDSiCCDS34440.1. [Q6UXB8-1]
RefSeqiNP_001186088.1. NM_001199159.1. [Q6UXB8-1]
NP_699201.2. NM_153370.2. [Q6UXB8-1]
XP_005248974.1. XM_005248917.1. [Q6UXB8-2]
XP_011512677.1. XM_011514375.1. [Q6UXB8-1]
UniGeneiHs.25391.

3D structure databases

ProteinModelPortaliQ6UXB8.
SMRiQ6UXB8. Positions 37-197.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000362778.

PTM databases

iPTMnetiQ6UXB8.
PhosphoSiteiQ6UXB8.

Polymorphism and mutation databases

BioMutaiPI16.
DMDMi74749419.

Proteomic databases

EPDiQ6UXB8.
MaxQBiQ6UXB8.
PaxDbiQ6UXB8.
PeptideAtlasiQ6UXB8.
PRIDEiQ6UXB8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000373674; ENSP00000362778; ENSG00000164530. [Q6UXB8-1]
ENST00000611814; ENSP00000478888; ENSG00000164530. [Q6UXB8-1]
GeneIDi221476.
KEGGihsa:221476.
UCSCiuc003ona.3. human. [Q6UXB8-1]

Organism-specific databases

CTDi221476.
GeneCardsiPI16.
HGNCiHGNC:21245. PI16.
HPAiHPA043763.
neXtProtiNX_Q6UXB8.
PharmGKBiPA134867616.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3017. Eukaryota.
COG2340. LUCA.
GeneTreeiENSGT00760000119004.
HOGENOMiHOG000115539.
HOVERGENiHBG104077.
InParanoidiQ6UXB8.
OMAiEGTPCSQ.
OrthoDBiEOG7F7WB4.
PhylomeDBiQ6UXB8.
TreeFamiTF316148.

Miscellaneous databases

GenomeRNAii221476.
PROiQ6UXB8.

Gene expression databases

BgeeiQ6UXB8.
CleanExiHS_PI16.
GenevisibleiQ6UXB8. HS.

Family and domain databases

Gene3Di3.40.33.10. 1 hit.
InterProiIPR001283. Allrgn_V5/Tpx1.
IPR018244. Allrgn_V5/Tpx1_CS.
IPR014044. CAP_domain.
[Graphical view]
PANTHERiPTHR10334. PTHR10334. 1 hit.
PfamiPF00188. CAP. 1 hit.
[Graphical view]
PRINTSiPR00837. V5TPXLIKE.
SMARTiSM00198. SCP. 1 hit.
[Graphical view]
SUPFAMiSSF55797. SSF55797. 1 hit.
PROSITEiPS01009. CRISP_1. 1 hit.
PS01010. CRISP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  2. "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries."
    Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y.
    , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T.
    DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT PRO-50.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT PRO-50.
    Tissue: Cerebellum.
  4. "The DNA sequence and analysis of human chromosome 6."
    Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
    Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT PRO-50.
    Tissue: Blood and Lung.
  6. "Identification, purification and characterization of a novel human blood protein with binding affinity for prostate secretory protein of 94 amino acids."
    Reeves J.R., Xuan J.W., Arfanis K., Morin C., Garde S.V., Ruiz M.T., Wisniewski J., Panchal C., Tanner J.E.
    Biochem. J. 385:105-114(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 28-57, GLYCOSYLATION, TISSUE SPECIFICITY, INTERACTION WITH MSMB.
  7. "Prognostic value of prostate secretory protein of 94 amino acids and its binding protein after radical prostatectomy."
    Reeves J.R., Dulude H., Panchal C., Daigneault L., Ramnani D.M.
    Clin. Cancer Res. 12:6018-6022(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  8. "Human urinary glycoproteomics; attachment site specific analysis of N-and O-linked glycosylations by CID and ECD."
    Halim A., Nilsson J., Ruetschi U., Hesse C., Larson G.
    Mol. Cell. Proteomics 0:0-0(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION, STRUCTURE OF CARBOHYDRATES, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiPI16_HUMAN
AccessioniPrimary (citable) accession number: Q6UXB8
Secondary accession number(s): Q6ZVG9
, Q8IYL8, Q8NBK0, Q8TCB8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: July 5, 2004
Last modified: June 8, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

May serve as a marker following prostatectomy for prostate cancer.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.