Q6UXB8 (PI16_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peptidase inhibitor 16
Short name=PI-16

Alternative name(s):
Cysteine-rich secretory protein 9
Short name=CRISP-9
PSP94-binding protein

Gene names

Name:	PI16
Synonyms:	CRISP9, PSPBP
ORF Names:	PSEC0164, UNQ289/PRO328

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Putative serine protease inhibitor By similarity.
Subunit structure	Interacts with PSP94/MSMB. Ref.6
Subcellular location	Membrane; Single-pass type I membrane protein Potential.
Tissue specificity	Expressed in prostate, testis, ovary and intestine. Concentrates in prostate cancer patient's sera. Ref.6 Ref.7
Post-translational modification	N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. Ref.6 Ref.8
Miscellaneous	May serve as a marker following prostatectomy for prostate cancer.
Sequence similarities	Belongs to the CRISP family. Contains 1 SCP domain.
Sequence caution	The sequence AAH22399.2 differs from that shown. Reason: Frameshift at position 400.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Protease inhibitor
PTM	Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	extracellular region Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	peptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6UXB8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6UXB8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     231-423: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Ref.6
	Chain	28 – 463	436	Peptidase inhibitor 16		PRO_0000287633
Regions
	Topological domain	28 – 442	415	Extracellular Potential
	Transmembrane	443 – 462	20	Helical; Potential
	Topological domain	463	1	Cytoplasmic Potential
	Domain	37 – 165	129	SCP
	Region	386 – 395	10	O-glycosylated at one site
Amino acid modifications
	Glycosylation	114	1	N-linked (GlcNAc...) Potential
	Glycosylation	403	1	N-linked (GlcNAc...) Potential
	Glycosylation	409	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	231 – 423	193	Missing in isoform 2.		VSP_025574
	Natural variant	50	1	T → P. Ref.2 Ref.3 Ref.5 Corresponds to variant rs1405069 [ dbSNP | Ensembl ].		VAR_032337
	Natural variant	416	1	L → V. Corresponds to variant rs16889318 [ dbSNP | Ensembl ].		VAR_032338
Experimental info
	Sequence conflict	179	1	E → G in BAC11640. Ref.2
	Sequence conflict	288	1	V → A in BAC11640. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 3B9E7565364CF0D2	FASTA	463	49,471
	10 20 30 40 50 60 MHGSCSFLML LLPLLLLLVA TTGPVGALTD EEKRLMVELH NLYRAQVSPT ASDMLHMRWD 70 80 90 100 110 120 EELAAFAKAY ARQCVWGHNK ERGRRGENLF AITDEGMDVP LAMEEWHHER EHYNLSAATC 130 140 150 160 170 180 SPGQMCGHYT QVVWAKTERI GCGSHFCEKL QGVEETNIEL LVCNYEPPGN VKGKRPYQEG 190 200 210 220 230 240 TPCSQCPSGY HCKNSLCEPI GSPEDAQDLP YLVTEAPSFR ATEASDSRKM GTPSSLATGI 250 260 270 280 290 300 PAFLVTEVSG SLATKALPAV ETQAPTSLAT KDPPSMATEA PPCVTTEVPS ILAAHSLPSL 310 320 330 340 350 360 DEEPVTFPKS THVPIPKSAD KVTDKTKVPS RSPENSLDPK MSLTGARELL PHAQEEAEAE 370 380 390 400 410 420 AELPPSSEVL ASVFPAQDKP GELQATLDHT GHTSSKSLPN FPNTSATANA TGGRALALQS 430 440 450 460 SLPGAEGPDK PSVVSGLNSG PGHVWGPLLG LLLLPPLVLA GIF
	Isoform 2 [UniParc]. Checksum: 04C0F6769E59B5C3	FASTA	270	29,674
	10 20 30 40 50 60 MHGSCSFLML LLPLLLLLVA TTGPVGALTD EEKRLMVELH NLYRAQVSPT ASDMLHMRWD 70 80 90 100 110 120 EELAAFAKAY ARQCVWGHNK ERGRRGENLF AITDEGMDVP LAMEEWHHER EHYNLSAATC 130 140 150 160 170 180 SPGQMCGHYT QVVWAKTERI GCGSHFCEKL QGVEETNIEL LVCNYEPPGN VKGKRPYQEG 190 200 210 220 230 240 TPCSQCPSGY HCKNSLCEPI GSPEDAQDLP YLVTEAPSFR ATEASDSRKM GAEGPDKPSV 250 260 270 VSGLNSGPGH VWGPLLGLLL LPPLVLAGIF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[2]	"Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries." Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y. , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T. DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT PRO-50.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT PRO-50. Tissue: Cerebellum.
[4]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT PRO-50. Tissue: Blood and Lung.
[6]	"Identification, purification and characterization of a novel human blood protein with binding affinity for prostate secretory protein of 94 amino acids." Reeves J.R., Xuan J.W., Arfanis K., Morin C., Garde S.V., Ruiz M.T., Wisniewski J., Panchal C., Tanner J.E. Biochem. J. 385:105-114(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 28-57, GLYCOSYLATION, TISSUE SPECIFICITY, INTERACTION WITH MSMB.
[7]	"Prognostic value of prostate secretory protein of 94 amino acids and its binding protein after radical prostatectomy." Reeves J.R., Dulude H., Panchal C., Daigneault L., Ramnani D.M. Clin. Cancer Res. 12:6018-6022(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[8]	"Human urinary glycoproteomics; attachment site specific analysis of N-and O-linked glycosylations by CID and ECD." Halim A., Nilsson J., Ruetschi U., Hesse C., Larson G. Mol. Cell. Proteomics 0:0-0(2011) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION, STRUCTURE OF CARBOHYDRATES, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY358422 mRNA. Translation: AAQ88788.1. AK075470 mRNA. Translation: BAC11640.1. AK124589 mRNA. Translation: BAC85892.1. AL122034 Genomic DNA. Translation: CAI23302.1. BC022399 mRNA. Translation: AAH22399.2. Frameshift. BC035634 mRNA. Translation: AAH35634.2.
IPI	IPI00301143. IPI00845506.
RefSeq	NP_001186088.1. NM_001199159.1. NP_699201.2. NM_153370.2.
UniGene	Hs.25391.
3D structure databases
HSSP	HSSP built from PDB template 1RC9 based on UniProtKB P60623.
ProteinModelPortal	Q6UXB8.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000362778.
PTM databases
PhosphoSite	Q6UXB8.
Polymorphism databases
DMDM	74749419.
Proteomic databases
PaxDb	Q6UXB8.
PeptideAtlas	Q6UXB8.
PRIDE	Q6UXB8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000373674; ENSP00000362778; ENSG00000164530. ENST00000536757; ENSP00000444848; ENSG00000164530.
GeneID	221476.
KEGG	hsa:221476.
UCSC	uc003omz.1. human. uc003ona.3. human.
Organism-specific databases
CTD	221476.
GeneCards	GC06P036555.
HGNC	HGNC:21245. PI16.
HPA	HPA043763.
neXtProt	NX_Q6UXB8.
PharmGKB	PA134867616.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG2340.
HOGENOM	HOG000115539.
HOVERGEN	HBG104077.
InParanoid	Q6UXB8.
OMA	EGTPCSQ.
OrthoDB	EOG4HMJBM.
Gene expression databases
ArrayExpress	Q6UXB8.
Bgee	Q6UXB8.
CleanEx	HS_PI16.
Genevestigator	Q6UXB8.
Family and domain databases
Gene3D	3.40.33.10. 1 hit.
InterPro	IPR001283. Allrgn_V5/Tpx1. IPR018244. Allrgn_V5/Tpx1_CS. IPR014044. CAP_domain. [Graphical view]
PANTHER	PTHR10334. PTHR10334. 1 hit.
Pfam	PF00188. CAP. 1 hit. [Graphical view]
PRINTS	PR00837. V5TPXLIKE.
SMART	SM00198. SCP. 1 hit. [Graphical view]
SUPFAM	SSF55797. SCP-like_extracellular. 1 hit.
PROSITE	PS01009. CRISP_1. 1 hit. PS01010. CRISP_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	221476.
NextBio	91359.

Entry information

Entry name

PI16_HUMAN

Accession

Primary (citable) accession number: Q6UXB8
Secondary accession number(s): Q6ZVG9 , Q8IYL8, Q8NBK0, Q8TCB8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.