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Protein

Butyrophilin-like protein 8

Gene

BTNL8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May stimulate primary immune response. Acts on T-cell stimulated sub-optimally through the TCR/CD3 complex stimulating their proliferation and cytokine production.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Adaptive immunity, Immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Butyrophilin-like protein 8
Gene namesi
Name:BTNL8
ORF Names:UNQ702/PRO1347
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:26131. BTNL8.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini18 – 238ExtracellularSequence analysisAdd BLAST221
Transmembranei239 – 259HelicalSequence analysisAdd BLAST21
Topological domaini260 – 500CytoplasmicSequence analysisAdd BLAST241

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000113303.
PharmGKBiPA134918466.

Polymorphism and mutation databases

BioMutaiBTNL8.
DMDMi59799184.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 171 PublicationAdd BLAST17
ChainiPRO_000001454118 – 500Butyrophilin-like protein 8Add BLAST483

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi40 ↔ 114PROSITE-ProRule annotation
Glycosylationi103N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi154 ↔ 206PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ6UX41.
PaxDbiQ6UX41.
PRIDEiQ6UX41.

PTM databases

iPTMnetiQ6UX41.
PhosphoSitePlusiQ6UX41.

Expressioni

Tissue specificityi

Expressed in neutrophils. Isoforms 1 and 5 are expressed at high levels in the colon, lung,testis, lymph nodes and thyroid tissue. Isoform 5, but not isoform 1, is detected in spleen.1 Publication

Inductioni

Down-regulated in neutrophils after treatment with LPS and TNF.1 Publication

Gene expression databases

BgeeiENSG00000113303.
CleanExiHS_BTNL8.
ExpressionAtlasiQ6UX41. baseline and differential.
GenevisibleiQ6UX41. HS.

Organism-specific databases

HPAiHPA039738.
HPA048494.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
TFF1P041553EBI-4314379,EBI-743871

Protein-protein interaction databases

BioGridi122991. 42 interactors.
IntActiQ6UX41. 1 interactor.

Structurei

3D structure databases

ProteinModelPortaliQ6UX41.
SMRiQ6UX41.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 132Ig-like V-type 1Add BLAST114
Domaini133 – 222Ig-like V-type 2Add BLAST90
Domaini270 – 466B30.2/SPRYPROSITE-ProRule annotationAdd BLAST197

Sequence similaritiesi

Contains 1 B30.2/SPRY domain.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IYU2. Eukaryota.
ENOG411140Z. LUCA.
GeneTreeiENSGT00760000118933.
HOGENOMiHOG000230860.
HOVERGENiHBG050747.
InParanoidiQ6UX41.
KOiK06712.
OMAiPYIEYPS.
OrthoDBiEOG091G0CXQ.
PhylomeDBiQ6UX41.
TreeFamiTF331083.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR001870. B30.2/SPRY.
IPR003879. Butyrophylin.
IPR013320. ConA-like_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
IPR006574. PRY.
IPR003877. SPRY_dom.
[Graphical view]
PfamiPF13765. PRY. 1 hit.
PF00622. SPRY. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
PRINTSiPR01407. BUTYPHLNCDUF.
SMARTiSM00409. IG. 2 hits.
SM00589. PRY. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
SSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS50835. IG_LIKE. 1 hit.
[Graphical view]

Sequences (7)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 7 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6UX41-1) [UniParc]FASTAAdd to basket
Also known as: BTN-like

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALMLSLVLS LLKLGSGQWQ VFGPDKPVQA LVGEDAAFSC FLSPKTNAEA
60 70 80 90 100
MEVRFFRGQF SSVVHLYRDG KDQPFMQMPQ YQGRTKLVKD SIAEGRISLR
110 120 130 140 150
LENITVLDAG LYGCRISSQS YYQKAIWELQ VSALGSVPLI SITGYVDRDI
160 170 180 190 200
QLLCQSSGWF PRPTAKWKGP QGQDLSTDSR TNRDMHGLFD VEISLTVQEN
210 220 230 240 250
AGSISCSMRH AHLSREVESR VQIGDTFFEP ISWHLATKVL GILCCGLFFG
260 270 280 290 300
IVGLKIFFSK FQWKIQAELD WRRKHGQAEL RDARKHAVEV TLDPETAHPK
310 320 330 340 350
LCVSDLKTVT HRKAPQEVPH SEKRFTRKSV VASQSFQAGK HYWEVDGGHN
360 370 380 390 400
KRWRVGVCRD DVDRRKEYVT LSPDHGYWVL RLNGEHLYFT LNPRFISVFP
410 420 430 440 450
RTPPTKIGVF LDYECGTISF FNINDQSLIY TLTCRFEGLL RPYIEYPSYN
460 470 480 490 500
EQNGTPIVIC PVTQESEKEA SWQRASAIPE TSNSESSSQA TTPFLPRGEM
Length:500
Mass (Da):56,748
Last modified:July 5, 2004 - v1
Checksum:i3314E96421533C74
GO
Isoform 2 (identifier: Q6UX41-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     263-347: WKIQAELDWR...AGKHYWEVDG → CKREREAWAG...ERRPETRSGG
     348-500: Missing.

Note: No experimental confirmation available.
Show »
Length:333
Mass (Da):36,733
Checksum:i14F6E13AB052026D
GO
Isoform 3 (identifier: Q6UX41-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     263-500: WKIQAELDWR...TTPFLPRGEM → CKREREAWAG...RDGSPEALRF

Note: No experimental confirmation available.
Show »
Length:340
Mass (Da):37,596
Checksum:i6B94F4F80E73C449
GO
Isoform 4 (identifier: Q6UX41-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-184: Missing.

Note: No experimental confirmation available.
Show »
Length:316
Mass (Da):36,222
Checksum:i2D45B9605F9999BD
GO
Isoform 5 (identifier: Q6UX41-5) [UniParc]FASTAAdd to basket
Also known as: B7-like

The sequence of this isoform differs from the canonical sequence as follows:
     263-347: WKIQAELDWR...AGKHYWEVDG → CKREREAWAG...RDGSPEALRF
     348-500: Missing.

Show »
Length:347
Mass (Da):38,209
Checksum:i12D617929E71A5E4
GO
Isoform 6 (identifier: Q6UX41-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     16-131: Missing.

Note: No experimental confirmation available.
Show »
Length:384
Mass (Da):43,609
Checksum:iCC0499D3FF7FD104
GO
Isoform 7 (identifier: Q6UX41-7) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-8: MALMLSLV → MWTWFVKS
     9-133: Missing.

Note: No experimental confirmation available.
Show »
Length:375
Mass (Da):42,933
Checksum:iA72C44E56A523161
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti311H → E in BAH12847 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_049839143T → A.1 PublicationCorresponds to variant rs2276995dbSNPEnsembl.1
Natural variantiVAR_049840229E → K.1 PublicationCorresponds to variant rs7724813dbSNPEnsembl.1
Natural variantiVAR_069135311H → Q.1 PublicationCorresponds to variant rs146303629dbSNPEnsembl.1
Natural variantiVAR_069136326T → K.1 PublicationCorresponds to variant rs7703365dbSNPEnsembl.1
Natural variantiVAR_069137477A → T.1 PublicationCorresponds to variant rs113820381dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0454711 – 184Missing in isoform 4. 1 PublicationAdd BLAST184
Alternative sequenceiVSP_0460671 – 8MALMLSLV → MWTWFVKS in isoform 7. 1 Publication8
Alternative sequenceiVSP_0460689 – 133Missing in isoform 7. 1 PublicationAdd BLAST125
Alternative sequenceiVSP_04606916 – 131Missing in isoform 6. 1 PublicationAdd BLAST116
Alternative sequenceiVSP_045472263 – 500WKIQA…PRGEM → CKREREAWAGALFMVPAGTG SEMLPHPAASLLLVLASRGP GPKKLEKKARTGRIERRPET RSGGDSGSRDGSPEALRF in isoform 3. 1 PublicationAdd BLAST238
Alternative sequenceiVSP_012718263 – 347WKIQA…WEVDG → CKREREAWAGALFMVPAGTG SEMLPHPAASLLLVLASRGP GPKKENPGGTGLEKKARTGR IERRPETRSGG in isoform 2. 1 PublicationAdd BLAST85
Alternative sequenceiVSP_045781263 – 347WKIQA…WEVDG → CKREREAWAGALFMVPAGTG SEMLPHPAASLLLVLASRGP GPKKENPGGTGLEKKARTGR IERRPETRSGGDSGSRDGSP EALRF in isoform 5. 1 PublicationAdd BLAST85
Alternative sequenceiVSP_012719348 – 500Missing in isoform 2 and isoform 5. 2 PublicationsAdd BLAST153

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY358523 mRNA. Translation: AAQ88887.1.
AK025111 mRNA. Translation: BAB15069.1.
AK296597 mRNA. Translation: BAH12395.1.
AK298687 mRNA. Translation: BAH12847.1.
AK225213 mRNA. No translation available.
AC022413 Genomic DNA. No translation available.
AC091874 Genomic DNA. No translation available.
CH471165 Genomic DNA. Translation: EAW53732.1.
BC119696 mRNA. Translation: AAI19697.1.
BC119697 mRNA. No translation available.
CCDSiCCDS43413.1. [Q6UX41-1]
CCDS4459.1. [Q6UX41-5]
CCDS54956.1. [Q6UX41-3]
CCDS54957.1. [Q6UX41-6]
CCDS54958.1. [Q6UX41-7]
CCDS54959.1. [Q6UX41-4]
RefSeqiNP_001035552.1. NM_001040462.2. [Q6UX41-1]
NP_001153179.1. NM_001159707.1. [Q6UX41-6]
NP_001153180.1. NM_001159708.1. [Q6UX41-3]
NP_001153181.1. NM_001159709.1. [Q6UX41-7]
NP_001153182.1. NM_001159710.1. [Q6UX41-4]
NP_079126.1. NM_024850.2. [Q6UX41-5]
UniGeneiHs.189109.

Genome annotation databases

EnsembliENST00000231229; ENSP00000231229; ENSG00000113303. [Q6UX41-5]
ENST00000340184; ENSP00000342197; ENSG00000113303. [Q6UX41-1]
ENST00000400707; ENSP00000383543; ENSG00000113303. [Q6UX41-7]
ENST00000508408; ENSP00000424585; ENSG00000113303. [Q6UX41-3]
ENST00000511704; ENSP00000425207; ENSG00000113303. [Q6UX41-6]
ENST00000533815; ENSP00000435098; ENSG00000113303. [Q6UX41-4]
ENST00000610640; ENSP00000483717; ENSG00000113303. [Q6UX41-7]
GeneIDi79908.
KEGGihsa:79908.
UCSCiuc003mmp.4. human. [Q6UX41-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY358523 mRNA. Translation: AAQ88887.1.
AK025111 mRNA. Translation: BAB15069.1.
AK296597 mRNA. Translation: BAH12395.1.
AK298687 mRNA. Translation: BAH12847.1.
AK225213 mRNA. No translation available.
AC022413 Genomic DNA. No translation available.
AC091874 Genomic DNA. No translation available.
CH471165 Genomic DNA. Translation: EAW53732.1.
BC119696 mRNA. Translation: AAI19697.1.
BC119697 mRNA. No translation available.
CCDSiCCDS43413.1. [Q6UX41-1]
CCDS4459.1. [Q6UX41-5]
CCDS54956.1. [Q6UX41-3]
CCDS54957.1. [Q6UX41-6]
CCDS54958.1. [Q6UX41-7]
CCDS54959.1. [Q6UX41-4]
RefSeqiNP_001035552.1. NM_001040462.2. [Q6UX41-1]
NP_001153179.1. NM_001159707.1. [Q6UX41-6]
NP_001153180.1. NM_001159708.1. [Q6UX41-3]
NP_001153181.1. NM_001159709.1. [Q6UX41-7]
NP_001153182.1. NM_001159710.1. [Q6UX41-4]
NP_079126.1. NM_024850.2. [Q6UX41-5]
UniGeneiHs.189109.

3D structure databases

ProteinModelPortaliQ6UX41.
SMRiQ6UX41.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122991. 42 interactors.
IntActiQ6UX41. 1 interactor.

PTM databases

iPTMnetiQ6UX41.
PhosphoSitePlusiQ6UX41.

Polymorphism and mutation databases

BioMutaiBTNL8.
DMDMi59799184.

Proteomic databases

MaxQBiQ6UX41.
PaxDbiQ6UX41.
PRIDEiQ6UX41.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000231229; ENSP00000231229; ENSG00000113303. [Q6UX41-5]
ENST00000340184; ENSP00000342197; ENSG00000113303. [Q6UX41-1]
ENST00000400707; ENSP00000383543; ENSG00000113303. [Q6UX41-7]
ENST00000508408; ENSP00000424585; ENSG00000113303. [Q6UX41-3]
ENST00000511704; ENSP00000425207; ENSG00000113303. [Q6UX41-6]
ENST00000533815; ENSP00000435098; ENSG00000113303. [Q6UX41-4]
ENST00000610640; ENSP00000483717; ENSG00000113303. [Q6UX41-7]
GeneIDi79908.
KEGGihsa:79908.
UCSCiuc003mmp.4. human. [Q6UX41-1]

Organism-specific databases

CTDi79908.
GeneCardsiBTNL8.
HGNCiHGNC:26131. BTNL8.
HPAiHPA039738.
HPA048494.
MIMi615606. gene.
neXtProtiNX_Q6UX41.
OpenTargetsiENSG00000113303.
PharmGKBiPA134918466.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IYU2. Eukaryota.
ENOG411140Z. LUCA.
GeneTreeiENSGT00760000118933.
HOGENOMiHOG000230860.
HOVERGENiHBG050747.
InParanoidiQ6UX41.
KOiK06712.
OMAiPYIEYPS.
OrthoDBiEOG091G0CXQ.
PhylomeDBiQ6UX41.
TreeFamiTF331083.

Miscellaneous databases

GenomeRNAii79908.
PROiQ6UX41.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000113303.
CleanExiHS_BTNL8.
ExpressionAtlasiQ6UX41. baseline and differential.
GenevisibleiQ6UX41. HS.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR001870. B30.2/SPRY.
IPR003879. Butyrophylin.
IPR013320. ConA-like_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
IPR006574. PRY.
IPR003877. SPRY_dom.
[Graphical view]
PfamiPF13765. PRY. 1 hit.
PF00622. SPRY. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
PRINTSiPR01407. BUTYPHLNCDUF.
SMARTiSM00409. IG. 2 hits.
SM00589. PRY. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
SSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS50835. IG_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBTNL8_HUMAN
AccessioniPrimary (citable) accession number: Q6UX41
Secondary accession number(s): A4QMZ8
, A6NEX6, B7Z409, B7Z5B1, E9PEF6, E9PG07, F2Z2B2, F8W712, Q9H730
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.