Q6UX15 (LAYN_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Layilin

Gene names

Name:	LAYN
ORF Names:	UNQ208/PRO234

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	382 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for hyaluronate. Ref.6
Subunit structure	Interacts with NF2, RDX and TLN1 By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein Potential. Note: Colocalizes with TLN1 at the membrane ruffles By similarity.
Domain	The C-terminal domain interacts with the N-terminal domain of RDX By similarity.
Sequence similarities	Contains 1 C-type lectin domain.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat Signal Transmembrane Transmembrane helix
Ligand	Lectin
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cell surface Inferred from sequence or structural similarity. Source: HGNC integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW ruffle Inferred from sequence or structural similarity. Source: HGNC
Molecular function	hyaluronic acid binding Inferred from direct assay Ref.6. Source: HGNC sugar binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6UX15-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6UX15-2)

The sequence of this isoform differs from the canonical sequence as follows:
     29-36: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 382	361	Layilin		PRO_0000262508
Regions
	Topological domain	22 – 235	214	Extracellular Potential
	Transmembrane	236 – 256	21	Helical; Potential
	Topological domain	257 – 382	126	Cytoplasmic Potential
	Domain	45 – 185	141	C-type lectin
	Repeat	340 – 344	5	1-1
	Repeat	350 – 354	5	1-2
	Repeat	356 – 359	4	2-1
	Repeat	371 – 375	5	1-3
	Repeat	377 – 380	4	2-2
	Region	330 – 374	45	Interaction with NF2 By similarity
	Region	337 – 382	46	Interaction with TLN1 By similarity
	Region	340 – 375	36	3 X 5 AA repeats of E-S-G-X-V
	Region	356 – 380	25	2 X 4 AA repeats of N-X-I-Y
Amino acid modifications
	Modified residue	286	1	Phosphoserine By similarity
	Modified residue	299	1	Phosphoserine By similarity
	Glycosylation	117	1	N-linked (GlcNAc...) Potential
	Disulfide bond	71 ↔ 184		By similarity
	Disulfide bond	150 ↔ 176		By similarity
Natural variations
	Alternative sequence	29 – 36	8	Missing in isoform 2.		VSP_021781
	Natural variant	66	1	E → K. Corresponds to variant rs11827718 [ dbSNP | Ensembl ].		VAR_029496
Experimental info
	Sequence conflict	219	1	A → T in BAB70978. Ref.2
	Sequence conflict	344	1	V → M in AAH25407. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 5843084E56E11325	FASTA	382	43,108
	10 20 30 40 50 60 MRPGTALQAV LLAVLLVGLR AATGRLLSAS DLDLRGGQPV CRGGTQRPCY KVIYFHDTSR 70 80 90 100 110 120 RLNFEEAKEA CRRDGGQLVS IESEDEQKLI EKFIENLLPS DGDFWIGLRR REEKQSNSTA 130 140 150 160 170 180 CQDLYAWTDG SISQFRNWYV DEPSCGSEVC VVMYHQPSAP AGIGGPYMFQ WNDDRCNMKN 190 200 210 220 230 240 NFICKYSDEK PAVPSREAEG EETELTTPVL PEETQEEDAK KTFKESREAA LNLAYILIPS 250 260 270 280 290 300 IPLLLLLVVT TVVCWVWICR KRKREQPDPS TKKQHTIWPS PHQGNSPDLE VYNVIRKQSE 310 320 330 340 350 360 ADLAETRPDL KNISFRVCSG EATPDDMSCD YDNMAVNPSE SGFVTLVSVE SGFVTNDIYE 370 380 FSPDQMGRSK ESGWVENEIY GY
	Isoform 2 [UniParc]. Checksum: 8AE64E6BC9E56DCD	FASTA	374	42,281
	10 20 30 40 50 60 MRPGTALQAV LLAVLLVGLR AATGRLLSGQ PVCRGGTQRP CYKVIYFHDT SRRLNFEEAK 70 80 90 100 110 120 EACRRDGGQL VSIESEDEQK LIEKFIENLL PSDGDFWIGL RRREEKQSNS TACQDLYAWT 130 140 150 160 170 180 DGSISQFRNW YVDEPSCGSE VCVVMYHQPS APAGIGGPYM FQWNDDRCNM KNNFICKYSD 190 200 210 220 230 240 EKPAVPSREA EGEETELTTP VLPEETQEED AKKTFKESRE AALNLAYILI PSIPLLLLLV 250 260 270 280 290 300 VTTVVCWVWI CRKRKREQPD PSTKKQHTIW PSPHQGNSPD LEVYNVIRKQ SEADLAETRP 310 320 330 340 350 360 DLKNISFRVC SGEATPDDMS CDYDNMAVNP SESGFVTLVS VESGFVTNDI YEFSPDQMGR 370 SKESGWVENE IYGY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[3]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed: 16554811] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[6]	"Layilin, a novel integral membrane protein, is a hyaluronan receptor." Bono P., Rubin K., Higgins J.M.G., Hynes R.O. Mol. Biol. Cell 12:891-900(2001) [PubMed: 11294894] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY358554 mRNA. Translation: AAQ88918.1. AK055539 mRNA. Translation: BAB70946.1. AK055654 mRNA. Translation: BAB70978.1. AP002008 Genomic DNA. No translation available. CH471065 Genomic DNA. Translation: EAW67145.1. BC025407 mRNA. Translation: AAH25407.1.
IPI	IPI00418125. IPI00744423.
RefSeq	NP_849156.1. NM_178834.3.
UniGene	Hs.503831.
3D structure databases
HSSP	HSSP built from PDB template 2H2R based on UniProtKB P06734.
ProteinModelPortal	Q6UX15.
SMR	Q6UX15. Positions 37-185.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6UX15.
PTM databases
PhosphoSite	Q6UX15.
Polymorphism databases
DMDM	74738165.
Proteomic databases
PRIDE	Q6UX15.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000375615; ENSP00000364765; ENSG00000204381.
GeneID	143903.
KEGG	hsa:143903.
UCSC	uc001plp.1. human. uc001plr.1. human.
Organism-specific databases
CTD	143903.
GeneCards	GC11P111411.
H-InvDB	HIX0010105.
HGNC	HGNC:29471. LAYN.
HPA	HPA040087.
neXtProt	NX_Q6UX15.
PharmGKB	PA142671570.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG08734.
GeneTree	ENSGT00390000001844.
HOGENOM	HBG715864.
HOVERGEN	HBG050945.
InParanoid	Q6UX15.
OMA	QGNSPDL.
Gene expression databases
ArrayExpress	Q6UX15.
Bgee	Q6UX15.
CleanEx	HS_LAYN.
Genevestigator	Q6UX15.
GermOnline	ENSG00000204381. Homo sapiens.
Family and domain databases
InterPro	IPR001304. C-type_lectin. IPR016186. C-type_lectin-like. IPR016187. C-type_lectin_fold. [Graphical view]
Gene3D	G3DSA:3.10.100.10. C-type_lectin-like. 1 hit.
KO	K10077.
Pfam	PF00059. Lectin_C. 1 hit. [Graphical view]
SMART	SM00034. CLECT. 1 hit. [Graphical view]
SUPFAM	SSF56436. C-type_lectin_fold. 1 hit.
PROSITE	PS00615. C_TYPE_LECTIN_1. False negative. PS50041. C_TYPE_LECTIN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	84809.

Entry information

Entry name

LAYN_HUMAN

Accession

Primary (citable) accession number: Q6UX15
Secondary accession number(s): A6NJB0 , Q8TAY8, Q96NC5, Q96NF3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.