Q6UX06 (OLFM4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Olfactomedin-4
Short name=OLM4

Alternative name(s):
Antiapoptotic protein GW112
G-CSF-stimulated clone 1 protein
Short name=hGC-1
hOLfD

Gene names

Name:	OLFM4
Synonyms:	GW112
ORF Names:	UNQ362/PRO698

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May promote proliferation of pancreatic cancer cells by favoring the transition from the S to G2/M phase. In myeloid leukemic cell lines, inhibits cell growth and induces cell differentiation and apoptosis. May play a role in the inhibition of EIF4EBP1 phosphorylation/deactivation. Facilitates cell adhesion, most probably through interaction with cell surface lectins and cadherin. Ref.7 Ref.8 Ref.9
Subunit structure	Homomultimer; disulfide-linked. Interacts with NDUFA13. Interacts with cell surface lectins (locutions ricinus communis agglutinin I, concanavalin-A and wheat germ agglutinin) and cadherin. Ref.6 Ref.7
Subcellular location	Secreted › extracellular space. Mitochondrion. Note: Subcellular location is not clearly defined: has been shown to be secreted (Ref.7), but also in the mitochondrion (Ref.6 and Ref.9), cytoplasm and plasma membrane (Ref.9) and in the nucleus (Ref.6). Ref.6 Ref.7 Ref.9
Tissue specificity	Expressed during myeloid lineage development. Much higher expression in bone marrow neutrophils than in peripheral blood neutrophils (at protein level). Strongly expressed in the prostate, small intestine and colon and moderately expressed in the bone marrow and stomach. Overexpressed in some pancreatic cancer tissues. Ref.5 Ref.7 Ref.8 Ref.9
Developmental stage	Elevated expression during the early S phase of the cell cycle, followed by a gradual decrease during late S phase. Ref.8
Induction	By retinoic acid. This induction requires functional NFKB pathway. Ref.9
Domain	The olfactomedin-like domain is involved in the interaction with cadherin. Ref.7
Post-translational modification	N-glycosylated.
Sequence similarities	Contains 1 olfactomedin-like domain.
Sequence caution	The sequence AAC72970.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAC72970.1 differs from that shown. Reason: Frameshift at several positions.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Mitochondrion Secreted
Coding sequence diversity	Polymorphism
Domain	Coiled coil Signal
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 510	490	Olfactomedin-4		PRO_0000311398
Regions
	Domain	245 – 507	263	Olfactomedin-like
	Coiled coil	155 – 234	80	Potential
	Compositional bias	31 – 71	41	Ser-rich
Amino acid modifications
	Glycosylation	72	1	N-linked (GlcNAc...) Potential
	Glycosylation	136	1	N-linked (GlcNAc...) Potential
	Glycosylation	253	1	N-linked (GlcNAc...) Potential
	Disulfide bond	246 ↔ 437		By similarity
Natural variations
	Natural variant	36	1	S → P. Corresponds to variant rs35790097 [ dbSNP | Ensembl ].		VAR_037246
Experimental info
	Mutagenesis	83	1	C → A: Abolishes secretion. No effect on multimer formation. Ref.7
	Mutagenesis	85	1	C → A: Abolishes secretion. No effect on multimer formation. Ref.7
	Mutagenesis	226	1	C → A: No effect on secretion. Affects multimer formation. Ref.7
	Mutagenesis	246	1	C → A: Abolishes secretion. No effect on multimer formation. Ref.7
	Mutagenesis	437	1	C → A: Abolishes secretion. No effect on multimer formation. Ref.7
	Sequence conflict	129	1	V → L in AAC72970. Ref.5
	Sequence conflict	304	1	R → I in AAC72970. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q6UX06 [UniParc]. Last modified April 12, 2005. Version 1. Checksum: ACF03365D2164900	FASTA	510	57,280
	10 20 30 40 50 60 MRPGLSFLLA LLFFLGQAAG DLGDVGPPIP SPGFSSFPGV DSSSSFSSSS RSGSSSSRSL 70 80 90 100 110 120 GSGGSVSQLF SNFTGSVDDR GTCQCSVSLP DTTFPVDRVE RLEFTAHVLS QKFEKELSKV 130 140 150 160 170 180 REYVQLISVY EKKLLNLTVR IDIMEKDTIS YTELDFELIK VEVKEMEKLV IQLKESFGGS 190 200 210 220 230 240 SEIVDQLEVE IRNMTLLVEK LETLDKNNVL AIRREIVALK TKLKECEASK DQNTPVVHPP 250 260 270 280 290 300 PTPGSCGHGG VVNISKPSVV QLNWRGFSYL YGAWGRDYSP QHPNKGLYWV APLNTDGRLL 310 320 330 340 350 360 EYYRLYNTLD DLLLYINARE LRITYGQGSG TAVYNNNMYV NMYNTGNIAR VNLTTNTIAV 370 380 390 400 410 420 TQTLPNAAYN NRFSYANVAW QDIDFAVDEN GLWVIYSTEA STGNMVISKL NDTTLQVLNT 430 440 450 460 470 480 WYTKQYKPSA SNAFMVCGVL YATRTMNTRT EEIFYYYDTN TGKEGKLDIV MHKMQEKVQS 490 500 510 INYNPFDQKL YVYNDGYLLN YDLSVLQKPQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	"The DNA sequence and analysis of human chromosome 13." Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T. Nature 428:522-528(2004) [PubMed: 15057823] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon and PNS.
[5]	"Identification and characterization of a novel member of olfactomedin-related protein family, hGC-1, expressed during myeloid lineage development." Zhang J., Liu W.-L., Tang D.C., Chen L., Wang M., Pack S.D., Zhuang Z., Rodgers G.P. Gene 283:83-93(2002) [PubMed: 11867215] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-510, TISSUE SPECIFICITY, PTM.
[6]	"GW112, a novel antiapoptotic protein that promotes tumor growth." Zhang X., Huang Q., Yang Z., Li Y., Li C.-Y. Cancer Res. 64:2474-2481(2004) [PubMed: 15059901] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH NDUFA13.
[7]	"The glycoprotein hGC-1 binds to cadherin and lectins." Liu W., Chen L., Zhu J., Rodgers G.P. Exp. Cell Res. 312:1785-1797(2006) [PubMed: 16566923] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DOMAIN, SUBUNIT, INTERACTION WITH CELL SURFACE LECTINS AND CADHERIN, MUTAGENESIS OF CYS-83; CYS-85; CYS-226; CYS-246 AND CYS-437.
[8]	"Olfactomedin 4 promotes S-phase transition in proliferation of pancreatic cancer cells." Kobayashi D., Koshida S., Moriai R., Tsuji N., Watanabe N. Cancer Sci. 98:334-340(2007) [PubMed: 17270022] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[9]	"Olfactomedin 4 is a novel target gene of retinoic acids and 5-aza-2'-deoxycytidine involved in human myeloid leukemia cell growth, differentiation, and apoptosis." Liu W., Lee H.W., Liu Y., Wang R., Rodgers G.P. Blood 116:4938-4947(2010) [PubMed: 20724538] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY358567 mRNA. Translation: AAQ88930.1. AL390736 Genomic DNA. Translation: CAH71311.2. CH471124 Genomic DNA. Translation: EAW52052.1. BC047740 mRNA. Translation: AAH47740.1. BC117329 mRNA. Translation: AAI17330.1. AF097021 mRNA. Translation: AAC72970.1. Sequence problems.
IPI	IPI00022255.
RefSeq	NP_006409.3. NM_006418.4.
UniGene	Hs.508113.
3D structure databases
ProteinModelPortal	Q6UX06.
SMR	Q6UX06. Positions 177-226.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q6UX06. 1 interaction.
STRING	Q6UX06.
PTM databases
PhosphoSite	Q6UX06.
Polymorphism databases
DMDM	74749412.
Proteomic databases
PRIDE	Q6UX06.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000219022; ENSP00000219022; ENSG00000102837.
GeneID	10562.
KEGG	hsa:10562.
UCSC	uc001vhl.1. human.
Organism-specific databases
CTD	10562.
GeneCards	GC13P053602.
H-InvDB	HIX0011348.
HGNC	HGNC:17190. OLFM4.
MIM	614061. gene.
neXtProt	NX_Q6UX06.
PharmGKB	PA134984745.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG19382.
GeneTree	ENSGT00560000077036.
HOGENOM	HBG713112.
HOVERGEN	HBG106662.
InParanoid	Q6UX06.
OMA	DYSPQHP.
OrthoDB	EOG4TQM92.
PhylomeDB	Q6UX06.
Gene expression databases
ArrayExpress	Q6UX06.
Bgee	Q6UX06.
CleanEx	HS_OLFM4.
Genevestigator	Q6UX06.
Family and domain databases
InterPro	IPR003112. Olfac-like. [Graphical view]
Pfam	PF02191. OLF. 1 hit. [Graphical view]
SMART	SM00284. OLF. 1 hit. [Graphical view]
PROSITE	PS51132. OLF. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	40085.
SOURCE	Search...

Entry information

Entry name

OLFM4_HUMAN

Accession

Primary (citable) accession number: Q6UX06
Secondary accession number(s): O95362, Q5VWG0, Q86T22

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	April 12, 2005
Last modified:	January 25, 2012
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.