Q6UX04 (CWC27_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peptidyl-prolyl cis-trans isomerase CWC27 homolog
Short name=PPIase CWC27
EC=5.2.1.8

Alternative name(s):
Antigen NY-CO-10
Serologically defined colon cancer antigen 10

Gene names

Name:	CWC27
Synonyms:	SDCCAG10
ORF Names:	UNQ438/PRO871

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	472 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	PPIases accelerate the folding of proteins By similarity.
Catalytic activity	Peptidylproline (omega=180) = peptidylproline (omega=0).
Sequence similarities	Belongs to the cyclophilin-type PPIase family. Contains 1 PPIase cyclophilin-type domain.
Sequence caution	The sequence AAC18041.1 differs from that shown. Reason: Frameshift at position 41. The sequence AAC18042.1 differs from that shown. Reason: Frameshift at position 136. The sequence AAH12117.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
Molecular function	Isomerase Rotamase
PTM	Glycoprotein Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mRNA splicing, via spliceosome Inferred by curator PubMed 11991638. Source: UniProtKB protein folding Inferred from electronic annotation. Source: UniProtKB-KW protein peptidyl-prolyl isomerization Inferred from electronic annotation. Source: GOC
Cellular_component	catalytic step 2 spliceosome Inferred from direct assay PubMed 11991638. Source: UniProtKB
Molecular_function	peptidyl-prolyl cis-trans isomerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6UX04-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6UX04-2)

The sequence of this isoform differs from the canonical sequence as follows:
     385-391: TLALLNQ → DVTCTS
     392-472: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 472

472

Peptidyl-prolyl cis-trans isomerase CWC27 homolog

PRO_0000313647

Regions

Domain

11 – 166

156

PPIase cyclophilin-type

Coiled coil

206 – 230

25

Potential

Coiled coil

306 – 377

72

Potential

Amino acid modifications

Modified residue

250

1

Phosphoserine By similarity

Glycosylation

109

1

N-linked (GlcNAc...) Potential

Glycosylation

201

1

N-linked (GlcNAc...) Ref.5

Natural variations

Alternative sequence

385 – 391

7

TLALLNQ → DVTCTS in isoform 2.

VSP_030082

Alternative sequence

392 – 472

81

Missing in isoform 2.

VSP_030083

Natural variant

256

1

P → A. Ref.1
Corresponds to variant rs7735338 [ dbSNP | Ensembl ].

VAR_037686

Experimental info

Sequence conflict

111 – 112

2

SQ → TH in AAC18041. Ref.1

Sequence conflict

331

1

K → E in AAC18042. Ref.1

Secondary structure

1

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472

Helix Strand Turn

Details...

Beta strand

14 – 19

6

Beta strand

22 – 29

8

Turn

30 – 32

3

Helix

34 – 45

12

Turn

46 – 51

6

Beta strand

56 – 58

3

Turn

59 – 61

3

Beta strand

62 – 65

4

Beta strand

70 – 73

4

Beta strand

95 – 101

7

Beta strand

113 – 118

6

Helix

121 – 123

3

Turn

124 – 126

3

Beta strand

129 – 133

5

Helix

135 – 137

3

Helix

138 – 143

6

Beta strand

155 – 157

3

Beta strand

160 – 168

9

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: BB0102157083439D	FASTA	472	53,847
	10 20 30 40 50 60 MSNIYIQEPP TNGKVLLKTT AGDIDIELWS KEAPKACRNF IQLCLEAYYD NTIFHRVVPG 70 80 90 100 110 120 FIVQGGDPTG TGSGGESIYG APFKDEFHSR LRFNRRGLVA MANAGSHDNG SQFFFTLGRA 130 140 150 160 170 180 DELNNKHTIF GKVTGDTVYN MLRLSEVDID DDERPHNPHK IKSCEVLFNP FDDIIPREIK 190 200 210 220 230 240 RLKKEKPEEE VKKLKPKGTK NFSLLSFGEE AEEEEEEVNR VSQSMKGKSK SSHDLLKDDP 250 260 270 280 290 300 HLSSVPVVES EKGDAPDLVD DGEDESAEHD EYIDGDEKNL MRERIAKKLK KDTSANVKSA 310 320 330 340 350 360 GEGEVEKKSV SRSEELRKEA RQLKRELLAA KQKKVENAAK QAEKRSEEEE APPDGAVAEY 370 380 390 400 410 420 RREKQKYEAL RKQQSKKGTS REDQTLALLN QFKSKLTQAI AETPENDIPE TEVEDDEGWM 430 440 450 460 470 SHVLQFEDKS RKVKDASMQD SDTFEIYDPR NPVNKRRREE SKKLMREKKE RR
	Isoform 2 [UniParc]. Checksum: 29010D816F06A71E	FASTA	390	43,989
	10 20 30 40 50 60 MSNIYIQEPP TNGKVLLKTT AGDIDIELWS KEAPKACRNF IQLCLEAYYD NTIFHRVVPG 70 80 90 100 110 120 FIVQGGDPTG TGSGGESIYG APFKDEFHSR LRFNRRGLVA MANAGSHDNG SQFFFTLGRA 130 140 150 160 170 180 DELNNKHTIF GKVTGDTVYN MLRLSEVDID DDERPHNPHK IKSCEVLFNP FDDIIPREIK 190 200 210 220 230 240 RLKKEKPEEE VKKLKPKGTK NFSLLSFGEE AEEEEEEVNR VSQSMKGKSK SSHDLLKDDP 250 260 270 280 290 300 HLSSVPVVES EKGDAPDLVD DGEDESAEHD EYIDGDEKNL MRERIAKKLK KDTSANVKSA 310 320 330 340 350 360 GEGEVEKKSV SRSEELRKEA RQLKRELLAA KQKKVENAAK QAEKRSEEEE APPDGAVAEY 370 380 390 RREKQKYEAL RKQQSKKGTS REDQDVTCTS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of human colon cancer antigens recognized by autologous antibodies." Scanlan M.J., Chen Y.-T., Williamson B., Gure A.O., Stockert E., Gordan J.D., Tuereci O., Sahin U., Pfreundschuh M., Old L.J. Int. J. Cancer 76:652-658(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT ALA-256. Tissue: Colon carcinoma.
[2]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Uterus.
[5]	"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry." Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D. J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-201, MASS SPECTROMETRY. Tissue: Plasma.
[6]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY358569 mRNA. Translation: AAQ88932.1.
CH471137 Genomic DNA. Translation: EAW51365.1.
AF039692 mRNA. Translation: AAC18041.1. Frameshift.
AF039693 mRNA. Translation: AAC18042.1. Frameshift.
BC012117 mRNA. Translation: AAH12117.1. Different initiation.

IPI

IPI00025174.
IPI00879475.

RefSeq

NP_005860.2. NM_005869.2.

UniGene

Hs.371372.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2HQ6	X-ray	1.75	A	8-173	[»]

ProteinModelPortal

Q6UX04.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q6UX04. 3 interactions.

STRING

9606.ENSP00000370460.

PTM databases

PhosphoSite

Q6UX04.

Polymorphism databases

DMDM

74749411.

Proteomic databases

PaxDb

Q6UX04.

PRIDE

Q6UX04.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000381070; ENSP00000370460; ENSG00000153015.

GeneID

10283.

KEGG

hsa:10283.

UCSC

uc003jtm.3. human.
uc010iwt.1. human.

Organism-specific databases

CTD

10283.

GeneCards

GC05P064064.

HGNC

HGNC:10664. CWC27.

HPA

HPA020344.
HPA024149.

neXtProt

NX_Q6UX04.

PharmGKB

PA35594.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG0652.

HOGENOM

HOG000161443.

HOVERGEN

HBG097993.

InParanoid

Q6UX04.

KO

K12737.

OMA

DASMQDS.

OrthoDB

EOG4GXFMV.

Gene expression databases

ArrayExpress

Q6UX04.

Bgee

Q6UX04.

CleanEx

HS_SDCCAG10.

Genevestigator

Q6UX04.

Family and domain databases

InterPro

IPR002130. Cyclophilin-like_PPIase_dom.
IPR020892. Cyclophilin-type_PPIase_CS.
[Graphical view]

Pfam

PF00160. Pro_isomerase. 1 hit.
[Graphical view]

PRINTS

PR00153. CSAPPISMRASE.

SUPFAM

SSF50891. CSA_PPIase. 1 hit.

PROSITE

PS00170. CSA_PPIASE_1. 1 hit.
PS50072. CSA_PPIASE_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q6UX04.

GenomeRNAi

10283.

NextBio

38960.

Entry information

Entry name

CWC27_HUMAN

Accession

Primary (citable) accession number: Q6UX04
Secondary accession number(s): O60529, O60530, Q96EM3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.