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Protein

Serine protease inhibitor Kazal-type 6

Gene

SPINK6

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Serine protease inhibitor selective for kallikreins. Efficiently inhibits KLK4, KLK5, KLK6, KLK7, KLK12, KLK13 and KLK14. Doesn't inhibit KLK8.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei42 – 432Reactive bondPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI01.015.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine protease inhibitor Kazal-type 6
Alternative name(s):
Kallikrein inhibitor
Gene namesi
Name:SPINK6
ORF Names:UNQ844/PRO1782
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:29486. SPINK6.

Subcellular locationi

  • Secreted By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134915474.

Polymorphism and mutation databases

BioMutaiSPINK6.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 23231 PublicationAdd
BLAST
Chaini24 – 8057Serine protease inhibitor Kazal-type 6PRO_0000016575Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei24 – 241Pyrrolidone carboxylic acidBy similarity
Disulfide bondi30 ↔ 62PROSITE-ProRule annotation
Disulfide bondi40 ↔ 59PROSITE-ProRule annotation
Disulfide bondi48 ↔ 80PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Proteomic databases

PaxDbiQ6UWN8.
PeptideAtlasiQ6UWN8.
PRIDEiQ6UWN8.

Expressioni

Gene expression databases

BgeeiQ6UWN8.
CleanExiHS_SPINK6.
ExpressionAtlasiQ6UWN8. baseline and differential.
GenevisibleiQ6UWN8. HS.

Organism-specific databases

HPAiHPA011039.

Interactioni

Protein-protein interaction databases

BioGridi135647. 1 interaction.
STRINGi9606.ENSP00000324870.

Structurei

Secondary structure

1
80
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi26 – 294Combined sources
Beta strandi34 – 374Combined sources
Beta strandi47 – 493Combined sources
Beta strandi54 – 574Combined sources
Helixi58 – 669Combined sources
Turni67 – 704Combined sources
Beta strandi74 – 785Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2N52NMR-A21-80[»]
ProteinModelPortaliQ6UWN8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 8057Kazal-likePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Kazal-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3649. Eukaryota.
ENOG410YC3T. LUCA.
GeneTreeiENSGT00530000064225.
HOGENOMiHOG000090244.
HOVERGENiHBG006182.
InParanoidiQ6UWN8.
OMAiFCFLTGV.
PhylomeDBiQ6UWN8.

Family and domain databases

InterProiIPR002350. Kazal_dom.
[Graphical view]
PfamiPF00050. Kazal_1. 1 hit.
[Graphical view]
SMARTiSM00280. KAZAL. 1 hit.
[Graphical view]
PROSITEiPS00282. KAZAL_1. 1 hit.
PS51465. KAZAL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6UWN8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLSGMFLLL SLALFCFLTG VFSQGGQVDC GEFQDPKVYC TRESNPHCGS
60 70 80
DGQTYGNKCA FCKAIVKSGG KISLKHPGKC
Length:80
Mass (Da):8,585
Last modified:July 5, 2004 - v1
Checksum:iC83CCA45AE69A900
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti36 – 361P → T.2 Publications
Corresponds to variant rs12186491 [ dbSNP | Ensembl ].
VAR_034020

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GQ504704 mRNA. Translation: ADM46526.1.
GQ504705 mRNA. Translation: ADM46527.1.
AY358716 mRNA. Translation: AAQ89078.1.
CH471062 Genomic DNA. Translation: EAW61813.1.
BC032003 mRNA. Translation: AAH32003.1.
CCDSiCCDS34268.1.
RefSeqiNP_001182219.1. NM_001195290.1.
NP_995313.2. NM_205841.3.
UniGeneiHs.334274.

Genome annotation databases

EnsembliENST00000325630; ENSP00000324870; ENSG00000178172.
ENST00000621437; ENSP00000481309; ENSG00000178172.
GeneIDi404203.
KEGGihsa:404203.
UCSCiuc003lpa.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GQ504704 mRNA. Translation: ADM46526.1.
GQ504705 mRNA. Translation: ADM46527.1.
AY358716 mRNA. Translation: AAQ89078.1.
CH471062 Genomic DNA. Translation: EAW61813.1.
BC032003 mRNA. Translation: AAH32003.1.
CCDSiCCDS34268.1.
RefSeqiNP_001182219.1. NM_001195290.1.
NP_995313.2. NM_205841.3.
UniGeneiHs.334274.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2N52NMR-A21-80[»]
ProteinModelPortaliQ6UWN8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi135647. 1 interaction.
STRINGi9606.ENSP00000324870.

Protein family/group databases

MEROPSiI01.015.

Polymorphism and mutation databases

BioMutaiSPINK6.

Proteomic databases

PaxDbiQ6UWN8.
PeptideAtlasiQ6UWN8.
PRIDEiQ6UWN8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000325630; ENSP00000324870; ENSG00000178172.
ENST00000621437; ENSP00000481309; ENSG00000178172.
GeneIDi404203.
KEGGihsa:404203.
UCSCiuc003lpa.4. human.

Organism-specific databases

CTDi404203.
GeneCardsiSPINK6.
HGNCiHGNC:29486. SPINK6.
HPAiHPA011039.
MIMi615868. gene.
neXtProtiNX_Q6UWN8.
PharmGKBiPA134915474.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3649. Eukaryota.
ENOG410YC3T. LUCA.
GeneTreeiENSGT00530000064225.
HOGENOMiHOG000090244.
HOVERGENiHBG006182.
InParanoidiQ6UWN8.
OMAiFCFLTGV.
PhylomeDBiQ6UWN8.

Miscellaneous databases

GenomeRNAii404203.
PROiQ6UWN8.
SOURCEiSearch...

Gene expression databases

BgeeiQ6UWN8.
CleanExiHS_SPINK6.
ExpressionAtlasiQ6UWN8. baseline and differential.
GenevisibleiQ6UWN8. HS.

Family and domain databases

InterProiIPR002350. Kazal_dom.
[Graphical view]
PfamiPF00050. Kazal_1. 1 hit.
[Graphical view]
SMARTiSM00280. KAZAL. 1 hit.
[Graphical view]
PROSITEiPS00282. KAZAL_1. 1 hit.
PS51465. KAZAL_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of SPINK6 in human skin: selective inhibitor of kallikrein-related peptidases."
    Meyer-Hoffert U., Wu Z., Kantyka T., Fischer J., Latendorf T., Hansmann B., Bartels J., He Y., Glaser R., Schroder J.M.
    J. Biol. Chem. 285:32174-32181(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, VARIANT THR-36.
    Tissue: Foreskin.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT THR-36.
    Tissue: Skin.
  5. "Signal peptide prediction based on analysis of experimentally verified cleavage sites."
    Zhang Z., Henzel W.J.
    Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 24-38.
  6. "Inhibition of kallikrein-related peptidases by the serine protease inhibitor of Kazal-type 6."
    Kantyka T., Fischer J., Wu Z., Declercq W., Reiss K., Schroder J.M., Meyer-Hoffert U.
    Peptides 32:1187-1192(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiISK6_HUMAN
AccessioniPrimary (citable) accession number: Q6UWN8
Secondary accession number(s): E0X656, Q8N5P0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: July 5, 2004
Last modified: July 6, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.