Reviewed, UniProtKB/Swiss-Prot Q6UWM7 (LCTL_HUMAN)

Names and origin

Protein names

Recommended name:
    Lactase-like protein
Alternative name(s):
    Klotho/lactase-phlorizin hydrolase-related protein

Gene names

Name:	LCTL
Synonyms:	KLPH
ORF Names:	UNQ3022/PRO9820

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	567 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.
Caution	Probably inactive as a glycosidase since it lacks the essential active site Glu at position 201.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Polymorphism
Domain	Signal Transmembrane
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Ref.2
	Chain	22 – 567	546	Lactase-like protein		PRO_0000042251
Regions
	Topological domain	23 – 541	519	Extracellular Potential
	Transmembrane	542 – 562	21	Potential
	Topological domain	563 – 567	5	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	80	1	N-linked (GlcNAc...) Potential
	Glycosylation	171	1	N-linked (GlcNAc...) Potential
	Glycosylation	245	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	212	1	T → M: dbSNP rs7179073.		VAR_049297
	Natural variant	240	1	A → T: dbSNP rs1030986.		VAR_023586

Sequences

	Sequence		Length	Mass (Da)
	Q6UWM7-1 [UniParc]. Last modified October 11, 2005. Version 2. Checksum: DA840A92E933C0AE	FASTA	567	65,088
	10 20 30 40 50 60 MKPVWVATLL WMLLLVPRLG AARKGSPEEA SFYYGTFPLG FSWGVGSSAY QTEGAWDQDG 70 80 90 100 110 120 KGPSIWDVFT HSGKGKVLGN ETADVACDGY YKVQEDIILL RELHVNHYRF SLSWPRLLPT 130 140 150 160 170 180 GIRAEQVNKK GIEFYSDLID ALLSSNITPI VTLHHWDLPQ LLQVKYGGWQ NVSMANYFRD 190 200 210 220 230 240 YANLCFEAFG DRVKHWITFS DPRAMAEKGY ETGHHAPGLK LRGTGLYKAA HHIIKAHAKA 250 260 270 280 290 300 WHSYNTTWRS KQQGLVGISL NCDWGEPVDI SNPKDLEAAE RYLQFCLGWF ANPIYAGDYP 310 320 330 340 350 360 QVMKDYIGRK SAEQGLEMSR LPVFSLQEKS YIKGTSDFLG LGHFTTRYIT ERNYPSRQGP 370 380 390 400 410 420 SYQNDRDLIE LVDPNWPDLG SKWLYSVPWG FRRLLNFAQT QYGDPPIYVM ENGASQKFHC 430 440 450 460 470 480 TQLCDEWRIQ YLKGYINEML KAIKDGANIK GYTSWSLLDK FEWEKGYSDR YGFYYVEFND 490 500 510 520 530 540 RNKPRYPKAS VQYYKKIIIA NGFPNPREVE SWYLKALETC SINNQMLAAE PLLSHMQMVT 550 560 EIVVPTVCSL CVLITAVLLM LLLRRQS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	"Signal peptide prediction based on analysis of experimentally verified cleavage sites." Zhang Z., Henzel W.J. Protein Sci. 13:2819-2824(2004) [PubMed: 15340161] [Abstract] Cited for: PROTEIN SEQUENCE OF 22-36.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY358729 mRNA. Translation: AAQ89091.1.
IPI	IPI00410421.
RefSeq	NP_997221.2.
UniGene	Hs.680983
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH1. Glycoside Hydrolase Family 1.
PTM databases
PhosphoSite	Q6UWM7.
Proteomic databases
PRIDE	Q6UWM7.
Genome annotation databases
Ensembl	ENSG00000188501. Homo sapiens. [Contig view]
GeneID	197021.
KEGG	hsa:197021.
Organism-specific databases
GeneCards	GC15M064628.
HGNC	HGNC:15583. LCTL.
PharmGKB	PA142671560.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q6UWM7.
HOVERGEN	Q6UWM7.
OMA	Q6UWM7. GFYYVEF.
Gene expression databases
ArrayExpress	Q6UWM7.
CleanEx	HS_LCTL.
GermOnline	ENSG00000188501. Homo sapiens.
Family and domain databases
InterPro	IPR001360. Glyco_hydro_1. IPR018120. Glyco_hydro_1_AS. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR10353. Glyco_hydro_1. 1 hit.
Pfam	PF00232. Glyco_hydro_1. 1 hit. [Graphical view]
PRINTS	PR00131. GLHYDRLASE1.
PROSITE	PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit. PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	89604.

Entry information

Entry name

LCTL_HUMAN

Accession

Primary (citable) accession number: Q6UWM7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2005
Last sequence update:	October 11, 2005
Last modified:	June 16, 2009
This is version 52 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)