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Q6UWB4 (PRS55_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine protease 55

EC=3.4.21.-
Alternative name(s):
Testis serine protease 1
Short name=T-SP1
Gene names
Name:PRSS55
Synonyms:TSP1
ORF Names:UNQ9391/PRO34284
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length352 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probable serine protease By similarity.

Subcellular location

Isoform 1: Membrane; Single-pass type I membrane protein Potential Ref.4.

Isoform 2: Cytoplasmcytosol Ref.4.

Tissue specificity

Only detected in testis. Expressed in spermatogonia, spermatocytes, spermatids, Leydig and Sertoli cells. Expressed in prostate cancer and ovarian cancer (at protein level). Ref.4 Ref.5

Sequence similarities

Belongs to the peptidase S1 family.

Contains 1 peptidase S1 domain.

Ontologies

Keywords
   Cellular componentCytoplasm
Membrane
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainSignal
Transmembrane
Transmembrane helix
   Molecular functionHydrolase
Protease
Serine protease
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytosol

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionserine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: Q6UWB4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q6UWB4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     248-352: GDSGGPLVCT...SHVLFRAILY → QSYFPTLQRMNTGSSQTKPPGSHTFHLQN

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Propeptide19 – 6749Activation propeptide By similarity
PRO_0000423643
Chain68 – 352285Serine protease 55
PRO_0000328815

Regions

Topological domain68 – 326259Extracellular Potential
Transmembrane327 – 34721Helical; Potential
Topological domain348 – 3525Cytoplasmic Potential
Domain68 – 300233Peptidase S1

Sites

Active site1081Charge relay system By similarity
Active site1561Charge relay system By similarity
Active site2501Charge relay system By similarity

Amino acid modifications

Glycosylation2401N-linked (GlcNAc...) Potential
Disulfide bond93 ↔ 109 By similarity
Disulfide bond189 ↔ 256 By similarity
Disulfide bond222 ↔ 235 By similarity
Disulfide bond246 ↔ 276 By similarity

Natural variations

Alternative sequence248 – 352105GDSGG…RAILY → QSYFPTLQRMNTGSSQTKPP GSHTFHLQN in isoform 2.
VSP_044557
Natural variant441P → R. Ref.1
Corresponds to variant rs4521726 [ dbSNP | Ensembl ].
VAR_042525
Natural variant2121A → V. Ref.1
Corresponds to variant rs4406360 [ dbSNP | Ensembl ].
VAR_042526

Experimental info

Sequence conflict151G → R in BC033497. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 18, 2010. Version 2.
Checksum: 0DBDFC385D5413A4

FASTA35238,856
        10         20         30         40         50         60 
MLLFSVLLLL SLVTGTQLGP RTPLPEAGVA ILGRARGAHR PQPPHPPSPV SECGDRSIFE 

        70         80         90        100        110        120 
GRTRYSRITG GMEAEVGEFP WQVSIQARSE PFCGGSILNK WWILTAAHCL YSEELFPEEL 

       130        140        150        160        170        180 
SVVLGTNDLT SPSMEIKEVA SIILHKDFKR ANMDNDIALL LLASPIKLDD LKVPICLPTQ 

       190        200        210        220        230        240 
PGPATWRECW VAGWGQTNAA DKNSVKTDLM KAPMVIMDWE ECSKMFPKLT KNMLCAGYKN 

       250        260        270        280        290        300 
ESYDACKGDS GGPLVCTPEP GEKWYQVGII SWGKSCGEKN TPGIYTSLVN YNLWIEKVTQ 

       310        320        330        340        350 
LEGRPFNAEK RRTSVKQKPM GSPVSGVPEP GSPRSWLLLC PLSHVLFRAI LY 

« Hide

Isoform 2 [UniParc].

Checksum: 67146392FCFD41CA
Show »

FASTA27630,559

References

« Hide 'large scale' references
[1]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS ARG-44 AND VAL-212.
[2]"DNA sequence and analysis of human chromosome 8."
Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. expand/collapse author list , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Brain.
[4]"T-SP1: a novel serine protease-like protein predominantly expressed in testis."
Neth P., Profanter B., Geissler C., Naegler D.K., Nerlich A., Sommerhoff C.P., Jochum M.
Biol. Chem. 389:1495-1504(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), ALTERNATIVE SPLICING, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[5]"Scanning of novel cancer/testis proteins by human testis proteomic analysis."
Liu M., Hu Z., Qi L., Wang J., Zhou T., Guo Y., Zeng Y., Zheng B., Wu Y., Zhang P., Chen X., Tu W., Zhang T., Zhou Q., Jiang M., Guo X., Zhou Z., Sha J.
Proteomics 13:1200-1210(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY358867 mRNA. Translation: AAQ89226.1.
AC104964 Genomic DNA. No translation available.
BC033497 mRNA. No translation available.
CCDSCCDS56523.1. [Q6UWB4-2]
CCDS5976.1. [Q6UWB4-1]
RefSeqNP_001183949.1. NM_001197020.1. [Q6UWB4-2]
NP_940866.2. NM_198464.3. [Q6UWB4-1]
UniGeneHs.591394.

3D structure databases

ProteinModelPortalQ6UWB4.
SMRQ6UWB4. Positions 68-300.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSS01.415.

Polymorphism databases

DMDM296453030.

Proteomic databases

PaxDbQ6UWB4.
PRIDEQ6UWB4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000328655; ENSP00000333003; ENSG00000184647. [Q6UWB4-1]
ENST00000522210; ENSP00000430459; ENSG00000184647. [Q6UWB4-2]
GeneID203074.
KEGGhsa:203074.
UCSCuc003wta.3. human. [Q6UWB4-1]

Organism-specific databases

CTD203074.
GeneCardsGC08P010383.
H-InvDBHIX0034319.
HGNCHGNC:30824. PRSS55.
HPAHPA027977.
MIM615144. gene.
neXtProtNX_Q6UWB4.
PharmGKBPA165585933.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5640.
HOGENOMHOG000251820.
HOVERGENHBG078102.
InParanoidQ6UWB4.
OMAPEPGEKW.
OrthoDBEOG7MKW6Q.
PhylomeDBQ6UWB4.
TreeFamTF338267.

Gene expression databases

ArrayExpressQ6UWB4.
BgeeQ6UWB4.
GenevestigatorQ6UWB4.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi203074.
NextBio90334.
PROQ6UWB4.
SOURCESearch...

Entry information

Entry namePRS55_HUMAN
AccessionPrimary (citable) accession number: Q6UWB4
Secondary accession number(s): E5RJX5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 18, 2010
Last modified: July 9, 2014
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 8

Human chromosome 8: entries, gene names and cross-references to MIM