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Protein

Regenerating islet-derived protein 3-gamma

Gene

REG3G

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Bactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. Restricts bacterial colonization of the intestinal epithelial surface and consequently limits activation of adaptive immune responses by the microbiota. The uncleaved form has bacteriostatic activity, whereas the cleaved form has bactericidal activity against L.monocytogenes and methicillin-resistant S.aureus. Regulates keratinocyte proliferation and differentiation after skin injury.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antimicrobial

Keywords - Biological processi

Acute phase, Inflammatory response

Keywords - Ligandi

Lectin

Names & Taxonomyi

Protein namesi
Recommended name:
Regenerating islet-derived protein 3-gamma
Short name:
REG-3-gamma
Alternative name(s):
Pancreatitis-associated protein 1B
Short name:
PAP-1B
Pancreatitis-associated protein IB
Short name:
PAP IB
Regenerating islet-derived protein III-gamma
Short name:
REG III
Short name:
Reg III-gamma
Cleaved into the following 2 chains:
Gene namesi
Name:REG3G
Synonyms:PAP1B
ORF Names:UNQ429/PRO162
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:29595. REG3G.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142671087.

Polymorphism and mutation databases

BioMutaiREG3G.
DMDMi68565895.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 26262 PublicationsAdd
BLAST
Chaini27 – 175149Regenerating islet-derived protein 3-gamma 16.5 kDa formPRO_0000017434Add
BLAST
Propeptidei27 – 37111 PublicationPRO_0000422751Add
BLAST
Chaini38 – 175138Regenerating islet-derived protein 3-gamma 15 kDa formPRO_0000422752Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi40 ↔ 51PROSITE-ProRule annotation
Disulfide bondi68 ↔ 171PROSITE-ProRule annotation
Disulfide bondi146 ↔ 163PROSITE-ProRule annotation

Post-translational modificationi

Proteolytic processing by trypsin removes an inhibitory N-terminal propeptide and is essential for peptidoglycan binding and antibacterial activity.1 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ6UW15.
PeptideAtlasiQ6UW15.
PRIDEiQ6UW15.

Expressioni

Tissue specificityi

Predominantly expressed in pancreas, where it may be restricted to exocrine pancreas. Moderate expression levels in testis and weak in heart, kidney and placenta.2 Publications

Gene expression databases

BgeeiQ6UW15.
CleanExiHS_REG3G.
GenevisibleiQ6UW15. HS.

Organism-specific databases

HPAiHPA048334.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000272324.

Structurei

3D structure databases

ProteinModelPortaliQ6UW15.
SMRiQ6UW15. Positions 40-175.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini47 – 172126C-type lectinPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi114 – 1163EPNBy similarity

Domaini

The EPN motif is essential for recognition of the peptidoglycan carbohydrate backbone and for efficient bacterial killing with Glu-114 playing a key role in peptidoglycan binding and bactericidal activity.By similarity

Sequence similaritiesi

Contains 1 C-type lectin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4297. Eukaryota.
ENOG410XPJ1. LUCA.
GeneTreeiENSGT00700000104249.
HOGENOMiHOG000010281.
HOVERGENiHBG004151.
InParanoidiQ6UW15.
OMAiILNPGHC.
OrthoDBiEOG738067.
PhylomeDBiQ6UW15.

Family and domain databases

Gene3Di3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR018378. C-type_lectin_CS.
IPR016187. C-type_lectin_fold.
[Graphical view]
PfamiPF00059. Lectin_C. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
PROSITEiPS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6UW15-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLPPMALPSV SWMLLSCLIL LCQVQGEETQ KELPSPRISC PKGSKAYGSP
60 70 80 90 100
CYALFLSPKS WMDADLACQK RPSGKLVSVL SGAEGSFVSS LVRSISNSYS
110 120 130 140 150
YIWIGLHDPT QGSEPDGDGW EWSSTDVMNY FAWEKNPSTI LNPGHCGSLS
160 170
RSTGFLKWKD YNCDAKLPYV CKFKD
Length:175
Mass (Da):19,330
Last modified:July 5, 2004 - v1
Checksum:iAF0F5FC59ACB90F5
GO
Isoform 2 (identifier: Q6UW15-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     66-111: Missing.

Show »
Length:129
Mass (Da):14,409
Checksum:iB5047BC23CFB243D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti174 – 1741K → T in CAG46736 (Ref. 4) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei66 – 11146Missing in isoform 2. 1 PublicationVSP_045169Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB161037 mRNA. Translation: BAD51394.1.
AB161038 mRNA. Translation: BAD51396.1.
AY428734 mRNA. Translation: AAR88147.1.
AY359047 mRNA. Translation: AAQ89406.1.
CR541938 mRNA. Translation: CAG46736.1.
AB161039 Genomic DNA. Translation: BAD51395.1.
AK292595 mRNA. Translation: BAF85284.1.
AC017004 Genomic DNA. Translation: AAX88840.1.
CH471053 Genomic DNA. Translation: EAW99580.1.
CH471053 Genomic DNA. Translation: EAW99581.1.
BC103852 mRNA. Translation: AAI03853.1.
BC103853 mRNA. Translation: AAI03854.1.
BC103854 mRNA. Translation: AAI03855.1.
CCDSiCCDS1962.1. [Q6UW15-1]
CCDS58714.1. [Q6UW15-2]
RefSeqiNP_001008388.1. NM_001008387.2. [Q6UW15-1]
NP_001256969.1. NM_001270040.1. [Q6UW15-2]
NP_940850.1. NM_198448.3. [Q6UW15-1]
XP_005264192.1. XM_005264135.1. [Q6UW15-1]
UniGeneiHs.447084.

Genome annotation databases

EnsembliENST00000272324; ENSP00000272324; ENSG00000143954. [Q6UW15-1]
ENST00000393897; ENSP00000377475; ENSG00000143954. [Q6UW15-1]
ENST00000409471; ENSP00000387105; ENSG00000143954. [Q6UW15-2]
GeneIDi130120.
KEGGihsa:130120.
UCSCiuc002snw.5. human. [Q6UW15-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB161037 mRNA. Translation: BAD51394.1.
AB161038 mRNA. Translation: BAD51396.1.
AY428734 mRNA. Translation: AAR88147.1.
AY359047 mRNA. Translation: AAQ89406.1.
CR541938 mRNA. Translation: CAG46736.1.
AB161039 Genomic DNA. Translation: BAD51395.1.
AK292595 mRNA. Translation: BAF85284.1.
AC017004 Genomic DNA. Translation: AAX88840.1.
CH471053 Genomic DNA. Translation: EAW99580.1.
CH471053 Genomic DNA. Translation: EAW99581.1.
BC103852 mRNA. Translation: AAI03853.1.
BC103853 mRNA. Translation: AAI03854.1.
BC103854 mRNA. Translation: AAI03855.1.
CCDSiCCDS1962.1. [Q6UW15-1]
CCDS58714.1. [Q6UW15-2]
RefSeqiNP_001008388.1. NM_001008387.2. [Q6UW15-1]
NP_001256969.1. NM_001270040.1. [Q6UW15-2]
NP_940850.1. NM_198448.3. [Q6UW15-1]
XP_005264192.1. XM_005264135.1. [Q6UW15-1]
UniGeneiHs.447084.

3D structure databases

ProteinModelPortaliQ6UW15.
SMRiQ6UW15. Positions 40-175.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000272324.

Polymorphism and mutation databases

BioMutaiREG3G.
DMDMi68565895.

Proteomic databases

PaxDbiQ6UW15.
PeptideAtlasiQ6UW15.
PRIDEiQ6UW15.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000272324; ENSP00000272324; ENSG00000143954. [Q6UW15-1]
ENST00000393897; ENSP00000377475; ENSG00000143954. [Q6UW15-1]
ENST00000409471; ENSP00000387105; ENSG00000143954. [Q6UW15-2]
GeneIDi130120.
KEGGihsa:130120.
UCSCiuc002snw.5. human. [Q6UW15-1]

Organism-specific databases

CTDi130120.
GeneCardsiREG3G.
HGNCiHGNC:29595. REG3G.
HPAiHPA048334.
MIMi609933. gene.
neXtProtiNX_Q6UW15.
PharmGKBiPA142671087.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4297. Eukaryota.
ENOG410XPJ1. LUCA.
GeneTreeiENSGT00700000104249.
HOGENOMiHOG000010281.
HOVERGENiHBG004151.
InParanoidiQ6UW15.
OMAiILNPGHC.
OrthoDBiEOG738067.
PhylomeDBiQ6UW15.

Miscellaneous databases

GeneWikiiREG3G.
GenomeRNAii130120.
PROiQ6UW15.
SOURCEiSearch...

Gene expression databases

BgeeiQ6UW15.
CleanExiHS_REG3G.
GenevisibleiQ6UW15. HS.

Family and domain databases

Gene3Di3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR018378. C-type_lectin_CS.
IPR016187. C-type_lectin_fold.
[Graphical view]
PfamiPF00059. Lectin_C. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
PROSITEiPS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning, expression and chromosomal localization of a novel human REG family gene, REG III."
    Nata K., Liu Y., Xu L., Ikeda T., Akiyama T., Noguchi N., Kawaguchi S., Yamauchi A., Takahashi I., Shervani N.J., Onogawa T., Takasawa S., Okamoto H.
    Gene 340:161-170(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Tissue: Pancreas.
  2. "PAP IB, a new member of the Reg gene family: cloning, expression, structural properties, and evolution by gene duplication."
    Laurine E., Manival X., Montgelard C., Bideau C., Berge-Lefranc J.-L., Erard M., Verdier J.-M.
    Biochim. Biophys. Acta 1727:177-187(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Testis.
  6. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
  9. "Regulation of C-type lectin antimicrobial activity by a flexible N-terminal prosegment."
    Mukherjee S., Partch C.L., Lehotzky R.E., Whitham C.V., Chu H., Bevins C.L., Gardner K.H., Hooper L.V.
    J. Biol. Chem. 284:4881-4888(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF N-TERMINUS, STRUCTURE BY NMR, FUNCTION, PROTEOLYTIC PROCESSING.
  10. "Signal peptide prediction based on analysis of experimentally verified cleavage sites."
    Zhang Z., Henzel W.J.
    Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 27-41.

Entry informationi

Entry nameiREG3G_HUMAN
AccessioniPrimary (citable) accession number: Q6UW15
Secondary accession number(s): A8K980
, D6W5J6, Q3SYE4, Q3SYE6, Q6FH18
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: July 5, 2004
Last modified: July 6, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.