ID   CP20A_HUMAN             Reviewed;         462 AA.
AC   Q6UW02; Q4ZG61; Q8N4Q8; Q8WWA9; Q9HC04;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   24-JAN-2024, entry version 157.
DE   RecName: Full=Cytochrome P450 20A1;
DE            EC=1.14.-.-;
GN   Name=CYP20A1; ORFNames=UNQ667/PRO1301;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), AND VARIANTS LEU-97 AND PHE-346.
RC   TISSUE=Pheochromocytoma;
RA   Peng Y., Li Y., Tu Y., Xu S., Han Z., Fu G., Chen Z.;
RT   "A novel gene expressed in human pheochromocytoma.";
RL   Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX   PubMed=12975309; DOI=10.1101/gr.1293003;
RA   Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA   Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA   Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA   Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA   Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA   Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA   Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA   Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT   "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT   identify novel human secreted and transmembrane proteins: a bioinformatics
RT   assessment.";
RL   Genome Res. 13:2265-2270(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15815621; DOI=10.1038/nature03466;
RA   Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA   Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA   Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA   Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA   Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA   Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA   Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA   Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA   Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA   McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA   Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA   Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA   Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA   Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA   Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA   Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA   Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA   Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA   Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA   Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA   Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA   Wilson R.K.;
RT   "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT   4.";
RL   Nature 434:724-731(2005).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANTS LEU-97 AND
RP   PHE-346.
RC   TISSUE=Blood, and Lung;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5]
RP   VARIANT [LARGE SCALE ANALYSIS] PHE-346, AND IDENTIFICATION BY MASS
RP   SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Liver;
RX   PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA   Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA   Ye M., Zou H.;
RT   "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT   phosphoproteome.";
RL   J. Proteomics 96:253-262(2014).
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q6UW02-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q6UW02-2; Sequence=VSP_055585;
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; AF183412; AAG09681.1; -; mRNA.
DR   EMBL; AY359068; AAQ89427.1; -; mRNA.
DR   EMBL; AC011737; AAX88915.1; -; Genomic_DNA.
DR   EMBL; BC020616; AAH20616.1; -; mRNA.
DR   EMBL; BC033752; AAH33752.1; -; mRNA.
DR   CCDS; CCDS2357.1; -. [Q6UW02-1]
DR   RefSeq; NP_803882.1; NM_177538.2. [Q6UW02-1]
DR   AlphaFoldDB; Q6UW02; -.
DR   SMR; Q6UW02; -.
DR   BioGRID; 121506; 24.
DR   IntAct; Q6UW02; 5.
DR   STRING; 9606.ENSP00000348380; -.
DR   iPTMnet; Q6UW02; -.
DR   PhosphoSitePlus; Q6UW02; -.
DR   BioMuta; CYP20A1; -.
DR   DMDM; 74749375; -.
DR   EPD; Q6UW02; -.
DR   jPOST; Q6UW02; -.
DR   MassIVE; Q6UW02; -.
DR   MaxQB; Q6UW02; -.
DR   PaxDb; 9606-ENSP00000348380; -.
DR   PeptideAtlas; Q6UW02; -.
DR   ProteomicsDB; 67441; -. [Q6UW02-1]
DR   Pumba; Q6UW02; -.
DR   Antibodypedia; 34163; 300 antibodies from 30 providers.
DR   DNASU; 57404; -.
DR   Ensembl; ENST00000356079.9; ENSP00000348380.4; ENSG00000119004.17. [Q6UW02-1]
DR   GeneID; 57404; -.
DR   KEGG; hsa:57404; -.
DR   MANE-Select; ENST00000356079.9; ENSP00000348380.4; NM_177538.3; NP_803882.1.
DR   UCSC; uc002uzv.5; human. [Q6UW02-1]
DR   AGR; HGNC:20576; -.
DR   CTD; 57404; -.
DR   DisGeNET; 57404; -.
DR   GeneCards; CYP20A1; -.
DR   HGNC; HGNC:20576; CYP20A1.
DR   HPA; ENSG00000119004; Low tissue specificity.
DR   neXtProt; NX_Q6UW02; -.
DR   OpenTargets; ENSG00000119004; -.
DR   PharmGKB; PA134911420; -.
DR   VEuPathDB; HostDB:ENSG00000119004; -.
DR   eggNOG; KOG0157; Eukaryota.
DR   GeneTree; ENSGT00500000044939; -.
DR   HOGENOM; CLU_050960_1_0_1; -.
DR   InParanoid; Q6UW02; -.
DR   OrthoDB; 3675424at2759; -.
DR   PhylomeDB; Q6UW02; -.
DR   TreeFam; TF105089; -.
DR   PathwayCommons; Q6UW02; -.
DR   SignaLink; Q6UW02; -.
DR   BioGRID-ORCS; 57404; 7 hits in 1156 CRISPR screens.
DR   ChiTaRS; CYP20A1; human.
DR   GeneWiki; CYP20A1; -.
DR   GenomeRNAi; 57404; -.
DR   Pharos; Q6UW02; Tbio.
DR   PRO; PR:Q6UW02; -.
DR   Proteomes; UP000005640; Chromosome 2.
DR   RNAct; Q6UW02; Protein.
DR   Bgee; ENSG00000119004; Expressed in calcaneal tendon and 150 other cell types or tissues.
DR   ExpressionAtlas; Q6UW02; baseline and differential.
DR   GO; GO:0016020; C:membrane; HDA:UniProtKB.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   CDD; cd20627; CYP20A1; 1.
DR   Gene3D; 1.10.630.10; Cytochrome P450; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   PANTHER; PTHR24280; CYTOCHROME P450 20A1; 1.
DR   PANTHER; PTHR24280:SF4; CYTOCHROME P450 20A1; 1.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   SUPFAM; SSF48264; Cytochrome P450; 1.
DR   Genevisible; Q6UW02; HS.
PE   1: Evidence at protein level;
KW   Alternative splicing; Heme; Iron; Membrane; Metal-binding; Monooxygenase;
KW   Oxidoreductase; Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..462
FT                   /note="Cytochrome P450 20A1"
FT                   /id="PRO_0000318095"
FT   TRANSMEM        4..24
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   BINDING         409
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
FT   VAR_SEQ         201..251
FT                   /note="VWSEIGKGFLDGSLDKNMTRKKQYEDALMQLESVLRNIIKERKGRNFSQHI
FT                   -> PSCNWSLFKEHHKRTKRKELQSTY (in isoform 2)"
FT                   /evidence="ECO:0000303|Ref.1"
FT                   /id="VSP_055585"
FT   VARIANT         97
FT                   /note="S -> L (in dbSNP:rs2043449)"
FT                   /evidence="ECO:0000269|PubMed:15489334, ECO:0000269|Ref.1"
FT                   /id="VAR_059153"
FT   VARIANT         346
FT                   /note="L -> F (in dbSNP:rs1048013)"
FT                   /evidence="ECO:0000269|PubMed:15489334, ECO:0000269|Ref.1,
FT                   ECO:0007744|PubMed:24275569"
FT                   /id="VAR_059154"
FT   CONFLICT        77
FT                   /note="L -> V (in Ref. 1; AAG09681)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        124
FT                   /note="K -> E (in Ref. 4; AAH20616)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        333..334
FT                   /note="TV -> LF (in Ref. 1; AAG09681)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   462 AA;  52432 MW;  4C0FEFFA0B9B055A CRC64;
     MLDFAIFAVT FLLALVGAVL YLYPASRQAA GIPGITPTEE KDGNLPDIVN SGSLHEFLVN
     LHERYGPVVS FWFGRRLVVS LGTVDVLKQH INPNKTSDPF ETMLKSLLRY QSGGGSVSEN
     HMRKKLYENG VTDSLKSNFA LLLKLSEELL DKWLSYPETQ HVPLSQHMLG FAMKSVTQMV
     MGSTFEDDQE VIRFQKNHGT VWSEIGKGFL DGSLDKNMTR KKQYEDALMQ LESVLRNIIK
     ERKGRNFSQH IFIDSLVQGN LNDQQILEDS MIFSLASCII TAKLCTWAIC FLTTSEEVQK
     KLYEEINQVF GNGPVTPEKI EQLRYCQHVL CETVRTAKLT PVSAQLQDIE GKIDRFIIPR
     ETLVLYALGV VLQDPNTWPS PHKFDPDRFD DELVMKTFSS LGFSGTQECP ELRFAYMVTT
     VLLSVLVKRL HLLSVEGQVI ETKYELVTSS REEAWITVSK RY
//