Q6UVJ0 (SAS6_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Spindle assembly abnormal protein 6 homolog
Short name=HsSAS-6

Gene names

Name:	SASS6
Synonyms:	SAS6

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	657 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for centrosome duplication. Overexpression results in excess foci-bearing centriolar markers. Ref.7 Ref.8 Ref.10
Subunit structure	Part of a ternary complex composed of SASS6, CENPJ and CEP350. Interacts with FBXW5. Ref.11 Ref.16
Subcellular location	Cytoplasm › cytoskeleton › centrosome. Cytoplasm › cytoskeleton › centrosome › centriole. Note: Component of the centrosome. Associated only transiently with nascent procentrioles during centriole biogenesis. Ref.5 Ref.6 Ref.7 Ref.10
Post-translational modification	Ubiquitinated by the SCF(FBXW5) E3 ubiquitin-protein ligase complex during S phase, leading to its degradation and preventing centriole reduplication. Ref.16
Sequence similarities	Contains 1 PISA domain.

Ontologies

Keywords
Biological process	Cell cycle
Cellular component	Cytoplasm Cytoskeleton
Coding sequence diversity	Polymorphism
Domain	Coiled coil
PTM	Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	centriole replication Inferred from mutant phenotype Ref.8Ref.10. Source: UniProtKB
Cellular_component	centriole Inferred from direct assay Ref.10. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 657	657	Spindle assembly abnormal protein 6 homolog		PRO_0000189972
Regions
	Domain	39 – 91	53	PISA
	Coiled coil	166 – 471	306	Potential
Amino acid modifications
	Modified residue	510	1	Phosphoserine Ref.12
	Modified residue	657	1	Phosphoserine Ref.12 Ref.14
Natural variations
	Natural variant	259	1	A → V. Corresponds to variant rs13375867 [ dbSNP | Ensembl ].		VAR_021590

Sequences

	Sequence		Length	Mass (Da)
	Q6UVJ0 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 6D19696BC748849F	FASTA	657	74,397
	10 20 30 40 50 60 MSQVLFHQLV PLQVKCKDCE ERRVSIRMSI ELQSVSNPVH RKDLVIRLTD DTDPFFLYNL 70 80 90 100 110 120 VISEEDFQSL KFQQGLLVDF LAFPQKFIDL LQQCTQEHAK EIPRFLLQLV SPAAILDNSP 130 140 150 160 170 180 AFLNVVETNP FKHLTHLSLK LLPGNDVEIK KFLAGCLKCS KEEKLSLMQS LDDATKQLDF 190 200 210 220 230 240 TRKTLAEKKQ ELDKLRNEWA SHTAALTNKH SQELTNEKEK ALQAQVQYQQ QHEQQKKDLE 250 260 270 280 290 300 ILHQQNIHQL QNRLSELEAA NKDLTERKYK GDSTIRELKA KLSGVEEELQ RTKQEVLSLR 310 320 330 340 350 360 RENSTLDVEC HEKEKHVNQL QTKVAVLEQE IKDKDQLVLR TKEAFDTIQE QKVVLEENGE 370 380 390 400 410 420 KNQVQLGKLE ATIKSLSAEL LKANEIIKKL QGDLKTLMGK LKLKNTVTIQ QEKLLAEKEE 430 440 450 460 470 480 KLQKEQKELQ DVGQSLRIKE QEVCKLQEQL EATVKKLEES KQLLKNNEKL ITWLNKELNE 490 500 510 520 530 540 NQLVRKQDVL GPSTTPPAHS SSNTIRSGIS PNLNVVDGRL TYPTCGIGYP VSSAFAFQNT 550 560 570 580 590 600 FPHSISAKNT SHPGSGTKVQ FNLQFTKPNA SLGDVQSGAT ISMPCSTDKE NGENVGLESK 610 620 630 640 650 YLKKREDSIP LRGLSQNLFS NSDHQRDGTL GALHTSSKPT ALPSASSAYF PGQLPNS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Shan Y.X., Chen K., Yu L. Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 153-657. Tissue: Amygdala.
[5]	"Proteomic characterization of the human centrosome by protein correlation profiling." Andersen J.S., Wilkinson C.J., Mayor T., Mortensen P., Nigg E.A., Mann M. Nature 426:570-574(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION, MASS SPECTROMETRY, SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. Tissue: Lymphoblast.
[6]	"Centriole assembly requires both centriolar and pericentriolar material proteins." Dammermann A., Mueller-Reichert T., Pelletier L., Habermann B., Desai A., Oegema K. Dev. Cell 7:815-829(2004) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[7]	"SAS-6 defines a protein family required for centrosome duplication in C. elegans and in human cells." Leidel S., Delattre M., Cerutti L., Baumer K., Goenczy P. Nat. Cell Biol. 7:115-125(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[8]	"The Polo kinase Plk4 functions in centriole duplication." Habedanck R., Stierhof Y.-D., Wilkinson C.J., Nigg E.A. Nat. Cell Biol. 7:1140-1146(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[9]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[10]	"Plk4-induced centriole biogenesis in human cells." Kleylein-Sohn J., Westendorf J., Le Clech M., Habedanck R., Stierhof Y.-D., Nigg E.A. Dev. Cell 13:190-202(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[11]	"Role of CAP350 in centriolar tubule stability and centriole assembly." Le Clech M. PLoS ONE 3:E3855-E3855(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN A COMPLEX WITH CENPJ AND CEP350.
[12]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-510 AND SER-657, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[13]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Leukemic T-cell.
[14]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-657, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[15]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[16]	"The SCF-FBXW5 E3-ubiquitin ligase is regulated by PLK4 and targets HsSAS-6 to control centrosome duplication." Puklowski A., Homsi Y., Keller D., May M., Chauhan S., Kossatz U., Grunwald V., Kubicka S., Pich A., Manns M.P., Hoffmann I., Gonczy P., Malek N.P. Nat. Cell Biol. 13:1004-1009(2011) [PubMed] [Europe PMC] [Abstract] Cited for: UBIQUITINATION, INTERACTION WITH FBXW5.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY359522 mRNA. Translation: AAQ57128.1. AL445928, AC093019 Genomic DNA. Translation: CAH72248.1. CH471097 Genomic DNA. Translation: EAW72974.1. CH471097 Genomic DNA. Translation: EAW72975.1. AL834265 mRNA. Translation: CAD38940.1.
IPI	IPI00375578.
RefSeq	NP_919268.1. NM_194292.1.
UniGene	Hs.591447.
3D structure databases
ProteinModelPortal	Q6UVJ0.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q6UVJ0. 4 interactions.
STRING	9606.ENSP00000287482.
PTM databases
PhosphoSite	Q6UVJ0.
Polymorphism databases
DMDM	62511032.
Proteomic databases
PaxDb	Q6UVJ0.
PeptideAtlas	Q6UVJ0.
PRIDE	Q6UVJ0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000287482; ENSP00000287482; ENSG00000156876.
GeneID	163786.
KEGG	hsa:163786.
UCSC	uc001dsu.3. human.
Organism-specific databases
CTD	163786.
GeneCards	GC01M100549.
HGNC	HGNC:25403. SASS6.
HPA	HPA028187.
MIM	609321. gene.
neXtProt	NX_Q6UVJ0.
PharmGKB	PA142670950.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG117813.
HOGENOM	HOG000124668.
HOVERGEN	HBG079167.
InParanoid	Q6UVJ0.
KO	K16487.
OMA	NIHQLQN.
OrthoDB	EOG4RNB8V.
PhylomeDB	Q6UVJ0.
Gene expression databases
ArrayExpress	Q6UVJ0.
Bgee	Q6UVJ0.
CleanEx	HS_SASS6.
Genevestigator	Q6UVJ0.
GermOnline	ENSG00000156876. Homo sapiens.
Family and domain databases
ProtoNet	Search...
Other
GenomeRNAi	163786.
NextBio	88409.
SOURCE	Search...

Entry information

Entry name

SAS6_HUMAN

Accession

Primary (citable) accession number: Q6UVJ0
Secondary accession number(s): D3DT55, Q8N3K0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 12, 2005
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.