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Protein

Myosin-14

Gene

Myh14

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi194 – 2018ATPSequence analysis

GO - Molecular functioni

  • actin-dependent ATPase activity Source: MGI
  • actin filament binding Source: MGI
  • ATP binding Source: MGI
  • microfilament motor activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Myosin

Keywords - Biological processi

Cell shape

Keywords - Ligandi

Actin-binding, ATP-binding, Calmodulin-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-5627123. RHO GTPases activate PAKs.

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin-14
Alternative name(s):
Myosin heavy chain 14
Myosin heavy chain, non-muscle IIc
Non-muscle myosin heavy chain IIc
Short name:
NMHC II-C
Gene namesi
Name:Myh14
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1919210. Myh14.

Subcellular locationi

GO - Cellular componenti

  • actomyosin Source: MGI
  • axon Source: MGI
  • brush border Source: UniProtKB
  • cytoplasm Source: MGI
  • extracellular exosome Source: MGI
  • growth cone Source: MGI
  • membrane Source: MGI
  • myelin sheath Source: UniProtKB
  • myosin complex Source: MGI
  • myosin II complex Source: MGI
  • myosin II filament Source: MGI
  • stress fiber Source: MGI
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Survival to adulthood with no obvious defects in brain structure, lung and heart development and no evidence for defective cell division. Deletion in animals expressing only 12% of wild-type amounts of Myh10 results in an increase in cardiac myocyte hypertrophy and interstitial fibrosis compared with the Myh10 hypomorphic animal.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 20001999Myosin-14PRO_0000123432Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei33 – 331PhosphothreonineCombined sources
Modified residuei56 – 561PhosphoserineBy similarity
Modified residuei925 – 9251PhosphoserineCombined sources
Modified residuei1198 – 11981PhosphothreonineBy similarity
Modified residuei1249 – 12491PhosphoserineCombined sources
Modified residuei1280 – 12801PhosphoserineCombined sources
Modified residuei1973 – 19731PhosphoserineCombined sources
Modified residuei1985 – 19851PhosphoserineBy similarity
Modified residuei1998 – 19981PhosphothreonineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ6URW6.
PaxDbiQ6URW6.
PeptideAtlasiQ6URW6.
PRIDEiQ6URW6.

PTM databases

iPTMnetiQ6URW6.
PhosphoSiteiQ6URW6.

Expressioni

Tissue specificityi

Highest levels in lung, kidney, brain and colon, very low levels in liver and bladder and no expression in spleen or seminal vesicle (at protein level). Isoform 1 is expressed in liver, kidney and testis with low levels in skeletal muscle and heart. Isoform 1 and isoform 2 are expressed in brain and lung. Isoform 2 is the main isoform expressed in skeletal muscle and heart. Isoform 3 is limited to brain stem, cerebellum and spinal cord.2 Publications

Developmental stagei

Widely expressed throughout the embryo at E11.5. Enhanced expression in the developing pituitary at E11.5. Expressed in developing lung from E13.5. At E16.5, confined to airway epithelial cells, developing sensory area of the cochlea and intestinal epithelial cells, particularly concentrated at their apical border.3 Publications

Gene expression databases

BgeeiENSMUSG00000030739.
CleanExiMM_MYH14.
ExpressionAtlasiQ6URW6. baseline and differential.
GenevisibleiQ6URW6. MM.

Interactioni

Subunit structurei

Myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).By similarity

GO - Molecular functioni

  • actin filament binding Source: MGI

Protein-protein interaction databases

IntActiQ6URW6. 1 interaction.
MINTiMINT-136587.
STRINGi10090.ENSMUSP00000103531.

Structurei

3D structure databases

ProteinModelPortaliQ6URW6.
SMRiQ6URW6. Positions 43-984.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini101 – 804704Myosin motorAdd
BLAST
Domaini807 – 83630IQPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili866 – 19511086Sequence analysisAdd
BLAST

Domaini

The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.By similarity

Sequence similaritiesi

Contains 1 IQ domain.PROSITE-ProRule annotation
Contains 1 myosin motor domain.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0161. Eukaryota.
COG5022. LUCA.
GeneTreeiENSGT00760000118919.
HOGENOMiHOG000173958.
HOVERGENiHBG004704.
InParanoidiQ6URW6.
KOiK10352.
OMAiQDRDEMA.
OrthoDBiEOG091G009J.
TreeFamiTF333601.

Family and domain databases

Gene3Di4.10.270.10. 1 hit.
InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR027401. Myosin-like_IQ_dom.
IPR001609. Myosin_head_motor_dom.
IPR004009. Myosin_N.
IPR002928. Myosin_tail.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00063. Myosin_head. 1 hit.
PF02736. Myosin_N. 1 hit.
PF01576. Myosin_tail_1. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00015. IQ. 1 hit.
SM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50096. IQ. 1 hit.
PS51456. MYOSIN_MOTOR. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6URW6-1) [UniParc]FASTAAdd to basket
Also known as: NM II-C1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAVTMSVSG RKVASRPGPV PEAAQSFLYA PRTPNVGGPG GPQVEWTARR
60 70 80 90 100
MVWVPSELHG FEAAALRDEG EEEAEVELAE SGRRLRLPRD QIQRMNPPKF
110 120 130 140 150
SKAEDMAELT CLNEASVLHN LRERYYSGLI YTYSGLFCVV INPYKQLPIY
160 170 180 190 200
TEAIVEMYRG KKRHEVPPHV YAVTEGAYRS MLQDREDQSI LCTGESGAGK
210 220 230 240 250
TENTKKVIQY LAHVASSPKG RKEPGVPASV STMSYGELER QLLQANPILE
260 270 280 290 300
AFGNAKTVKN DNSSRFGKFI RINFDIAGYI VGANIETYLL EKSRAIRQAK
310 320 330 340 350
DECSFHIFYQ LLGGAGEQLK ADLLLEPCSH YRFLTNGPSS SPGQERELFQ
360 370 380 390 400
ETLESLRVLG LLPEEITAML RTVSAVLQFG NIVLKKERNT DQATMPDNTA
410 420 430 440 450
AQKLCRLLGL GVTDFSRALL TPRIKVGRDY VQKAQTKEQA DFALEALAKA
460 470 480 490 500
TYERLFRWLV LRLNRALDRS PRQGASFLGI LDIAGFEIFQ LNSFEQLCIN
510 520 530 540 550
YTNEKLQQLF NHTMFVLEQE EYQREGIPWT FLDFGLDLQP CIDLIERPAN
560 570 580 590 600
PPGLLALLDE ECWFPKATDK SFVEKVAQEQ GSHPKFQRPR NLRDQADFSV
610 620 630 640 650
LHYAGKVDYK ASEWLMKNMD PLNDNVAALL HQSTDRLTAE IWKDVEGIVG
660 670 680 690 700
LEQVSSLGDG PPGGRPRRGM FRTVGQLYKE SLSRLMATLS NTNPSFVRCI
710 720 730 740 750
VPNHEKRAGK LEPRLVLDQL RCNGVLEGIR ICRQGFPNRI LFQEFRQRYE
760 770 780 790 800
ILTPNAIPKG FMDGKQACEK MIQALELDPN LYRVGQSKIF FRAGVLAQLE
810 820 830 840 850
EERDLKVTDI IVSFQAAARG YLARRAFQRR QQQQSALRVM QRNCAAYLKL
860 870 880 890 900
RNWQWWRLFI KVKPLLQVTR QDEVLQARAQ ELQKVQELQQ QSAREVGELQ
910 920 930 940 950
GRVAQLEEER TRLAEQLRAE AELCSEAEET RARLAARKQE LELVVTELEA
960 970 980 990 1000
RVGEEEECSR QLQSEKKRLQ QHIQELESHL EAEEGARQKL QLEKVTTEAK
1010 1020 1030 1040 1050
MKKFEEDLLL LEDQNSKLSK ERRLLEERLA EFSSQAAEEE EKVKSLNKLR
1060 1070 1080 1090 1100
LKYEATISDM EDRLKKEEKG RQELEKLKRR LDGESSELQE QMVEQKQRAE
1110 1120 1130 1140 1150
ELLAQLGRKE DELQAALLRA EEEGGARAQL LKSLREAQAG LAEAQEDLEA
1160 1170 1180 1190 1200
ERVARAKAEK QRRDLGEELE ALRGELEDTL DSTNAQQELR SKREQEVTEL
1210 1220 1230 1240 1250
KKALEEESRA HEVSMQELRQ RHSQALVEMA EQLEQARRGK GVWEKTRLSL
1260 1270 1280 1290 1300
EAEVSELKAE LSSLQTSRQE GEQKRRRLES QLQEVQGRSS DSERARSEAA
1310 1320 1330 1340 1350
EKLQRAQAEL ESVSTALSEA ESKAIRLGKE LSSAESQLHD TQELLQEETR
1360 1370 1380 1390 1400
AKLALGSRVR ALEAEAAGLR EQMEEEVVAR ERAGRELQST QAQLSEWRRR
1410 1420 1430 1440 1450
QEEEAAVLEA GEEARRRAAR EAETLTQRLA EKTEAVERLE RARRRLQQEL
1460 1470 1480 1490 1500
DDATVDLGQQ KQLLSTLEKK QRKFDQLLAE EKAAVLRAVE DRERIEAEGR
1510 1520 1530 1540 1550
EREARALSLT RALEEEQEAR EELERQNRAL RAELEALLSS KDDVGKNVHE
1560 1570 1580 1590 1600
LERARKAAEQ AASDLRTQVT ELEDELTAAE DAKLRLEVTV QALKAQHERD
1610 1620 1630 1640 1650
LQGRDDAGEE RRRQLAKQLR DAEVERDEER KQRALAMAAR KKLELELEEL
1660 1670 1680 1690 1700
KAQTSAAGQG KEEAVKQLKK MQVQMKELWR EVEETRSSRD EMFTLSRENE
1710 1720 1730 1740 1750
KKLKGLEAEV LRLQEELAAS DRARRQAQQD RDEMAEEVAS GNLSKAATLE
1760 1770 1780 1790 1800
EKRQLEGRLS QLEEELEEEQ NNSELLKDHY RKLVLQVESL TTELSAERSF
1810 1820 1830 1840 1850
SAKAESGRQQ LERQIQELRA RLGEEDAGAR ARQKMLIAAL ESKLAQAEEQ
1860 1870 1880 1890 1900
LEQESRERIL SGKLVRRAEK RLKEVVLQVD EERRVADQVR DQLEKSNLRL
1910 1920 1930 1940 1950
KQLKRQLEEA EEEASRAQAG RRRLQRELED VTESAESMNR EVTTLRNRLR
1960 1970 1980 1990 2000
RGPLTFTTRT VRQVFRLEEG VASDEEEAEG AEPGSAPGQE PEAPPPATPQ
Note: Requires phosphorylation of the myosin regulatory light chain for activity.
Length:2,000
Mass (Da):228,586
Last modified:July 5, 2004 - v1
Checksum:i1957C1A42EF2A21A
GO
Isoform 2 (identifier: Q6URW6-2) [UniParc]FASTAAdd to basket
Also known as: NM II-C0

The sequence of this isoform differs from the canonical sequence as follows:
     228-235: Missing.

Note: Requires phosphorylation of the myosin regulatory light chain for activity.
Show »
Length:1,992
Mass (Da):227,759
Checksum:i871EF7B8752D6614
GO
Isoform 3 (identifier: Q6URW6-3) [UniParc]FASTAAdd to basket
Also known as: NM II-C2

The sequence of this isoform differs from the canonical sequence as follows:
     228-235: Missing.
     644-644: D → DEQGGLQQFTLLGSFPSPSPGPAGRLGSGASPPGVGSLCAPT

Note: Constitutively active isoform which does not require phosphorylation of the regulatory myosin light chain for activity.
Show »
Length:2,033
Mass (Da):231,622
Checksum:i2362751C95BFB052
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei228 – 2358Missing in isoform 2 and isoform 3. 2 PublicationsVSP_014633
Alternative sequencei644 – 6441D → DEQGGLQQFTLLGSFPSPSP GPAGRLGSGASPPGVGSLCA PT in isoform 3. 1 PublicationVSP_044759

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY363100 mRNA. Translation: AAQ24173.1.
AY205605 mRNA. Translation: AAO47092.1.
EF602040 mRNA. Translation: ABR10605.1.
AC150895 Genomic DNA. No translation available.
AC157653 Genomic DNA. No translation available.
BC041796 mRNA. Translation: AAH41796.1.
CCDSiCCDS39943.1. [Q6URW6-2]
CCDS71951.1. [Q6URW6-1]
RefSeqiNP_001258467.1. NM_001271538.1. [Q6URW6-3]
NP_001258469.1. NM_001271540.1. [Q6URW6-1]
NP_082297.1. NM_028021.3. [Q6URW6-2]
XP_011249204.1. XM_011250902.1. [Q6URW6-2]
XP_011249205.1. XM_011250903.1. [Q6URW6-1]
UniGeneiMm.158289.

Genome annotation databases

EnsembliENSMUST00000048102; ENSMUSP00000046059; ENSMUSG00000030739. [Q6URW6-1]
ENSMUST00000107899; ENSMUSP00000103531; ENSMUSG00000030739. [Q6URW6-2]
ENSMUST00000207775; ENSMUSP00000147115; ENSMUSG00000030739. [Q6URW6-3]
GeneIDi71960.
KEGGimmu:71960.
UCSCiuc009gqi.2. mouse. [Q6URW6-2]
uc009gqj.2. mouse. [Q6URW6-1]
uc012fjt.2. mouse. [Q6URW6-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY363100 mRNA. Translation: AAQ24173.1.
AY205605 mRNA. Translation: AAO47092.1.
EF602040 mRNA. Translation: ABR10605.1.
AC150895 Genomic DNA. No translation available.
AC157653 Genomic DNA. No translation available.
BC041796 mRNA. Translation: AAH41796.1.
CCDSiCCDS39943.1. [Q6URW6-2]
CCDS71951.1. [Q6URW6-1]
RefSeqiNP_001258467.1. NM_001271538.1. [Q6URW6-3]
NP_001258469.1. NM_001271540.1. [Q6URW6-1]
NP_082297.1. NM_028021.3. [Q6URW6-2]
XP_011249204.1. XM_011250902.1. [Q6URW6-2]
XP_011249205.1. XM_011250903.1. [Q6URW6-1]
UniGeneiMm.158289.

3D structure databases

ProteinModelPortaliQ6URW6.
SMRiQ6URW6. Positions 43-984.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ6URW6. 1 interaction.
MINTiMINT-136587.
STRINGi10090.ENSMUSP00000103531.

PTM databases

iPTMnetiQ6URW6.
PhosphoSiteiQ6URW6.

Proteomic databases

MaxQBiQ6URW6.
PaxDbiQ6URW6.
PeptideAtlasiQ6URW6.
PRIDEiQ6URW6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000048102; ENSMUSP00000046059; ENSMUSG00000030739. [Q6URW6-1]
ENSMUST00000107899; ENSMUSP00000103531; ENSMUSG00000030739. [Q6URW6-2]
ENSMUST00000207775; ENSMUSP00000147115; ENSMUSG00000030739. [Q6URW6-3]
GeneIDi71960.
KEGGimmu:71960.
UCSCiuc009gqi.2. mouse. [Q6URW6-2]
uc009gqj.2. mouse. [Q6URW6-1]
uc012fjt.2. mouse. [Q6URW6-3]

Organism-specific databases

CTDi79784.
MGIiMGI:1919210. Myh14.

Phylogenomic databases

eggNOGiKOG0161. Eukaryota.
COG5022. LUCA.
GeneTreeiENSGT00760000118919.
HOGENOMiHOG000173958.
HOVERGENiHBG004704.
InParanoidiQ6URW6.
KOiK10352.
OMAiQDRDEMA.
OrthoDBiEOG091G009J.
TreeFamiTF333601.

Enzyme and pathway databases

ReactomeiR-MMU-5627123. RHO GTPases activate PAKs.

Miscellaneous databases

ChiTaRSiMyh14. mouse.
PROiQ6URW6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030739.
CleanExiMM_MYH14.
ExpressionAtlasiQ6URW6. baseline and differential.
GenevisibleiQ6URW6. MM.

Family and domain databases

Gene3Di4.10.270.10. 1 hit.
InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR027401. Myosin-like_IQ_dom.
IPR001609. Myosin_head_motor_dom.
IPR004009. Myosin_N.
IPR002928. Myosin_tail.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00063. Myosin_head. 1 hit.
PF02736. Myosin_N. 1 hit.
PF01576. Myosin_tail_1. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00015. IQ. 1 hit.
SM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50096. IQ. 1 hit.
PS51456. MYOSIN_MOTOR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMYH14_MOUSE
AccessioniPrimary (citable) accession number: Q6URW6
Secondary accession number(s): B3F3T1, Q80V64, Q80ZE6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 5, 2004
Last modified: September 7, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.