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Protein

Heterogeneous nuclear ribonucleoprotein A3

Gene

Hnrnpa3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in cytoplasmic trafficking of RNA. Binds to the cis-acting response element, A2RE. May be involved in pre-mRNA splicing.1 Publication

GO - Molecular functioni

  • mRNA binding Source: RGD
  • nucleotide binding Source: InterPro
  • RNA transmembrane transporter activity Source: RGD

GO - Biological processi

  • mRNA processing Source: UniProtKB-KW
  • mRNA transport Source: RGD
  • RNA splicing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-RNO-72163. mRNA Splicing - Major Pathway.
R-RNO-72203. Processing of Capped Intron-Containing Pre-mRNA.

Names & Taxonomyi

Protein namesi
Recommended name:
Heterogeneous nuclear ribonucleoprotein A3
Short name:
hnRNP A3
Gene namesi
Name:Hnrnpa3
Synonyms:Hnrpa3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi727807. Hnrnpa3.

Subcellular locationi

  • Nucleus By similarity

  • Note: Component of ribonucleosomes.By similarity

GO - Cellular componenti

  • catalytic step 2 spliceosome Source: Ensembl
  • cytoplasm Source: RGD
  • messenger ribonucleoprotein complex Source: RGD
  • neuron projection Source: RGD
  • nucleoplasm Source: Ensembl
  • nucleus Source: RGD
  • ribonucleoprotein granule Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 379379Heterogeneous nuclear ribonucleoprotein A3PRO_0000081840Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Cross-linki4 – 4Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei14 – 141PhosphoserineBy similarity
Modified residuei43 – 431PhosphoserineBy similarity
Modified residuei52 – 521Dimethylated arginine; alternateBy similarity
Modified residuei52 – 521Omega-N-methylarginine; alternateBy similarity
Modified residuei116 – 1161PhosphoserineBy similarity
Modified residuei124 – 1241PhosphothreonineBy similarity
Modified residuei134 – 1341N6-acetyllysineBy similarity
Modified residuei214 – 2141Asymmetric dimethylarginine1 Publication
Modified residuei216 – 2161Asymmetric dimethylarginine1 Publication
Modified residuei226 – 2261Asymmetric dimethylarginine1 Publication
Modified residuei239 – 2391Asymmetric dimethylarginine1 Publication
Modified residuei246 – 2461Asymmetric dimethylarginine1 Publication
Modified residuei286 – 2861Asymmetric dimethylarginine1 Publication
Modified residuei351 – 3511PhosphoserineBy similarity
Modified residuei359 – 3591PhosphoserineCombined sources
Modified residuei361 – 3611PhosphotyrosineBy similarity
Modified residuei365 – 3651PhosphotyrosineBy similarity
Modified residuei367 – 3671PhosphoserineBy similarity
Modified residuei371 – 3711PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ6URK4.
PRIDEiQ6URK4.

PTM databases

iPTMnetiQ6URK4.
PhosphoSiteiQ6URK4.

Expressioni

Gene expression databases

GenevisibleiQ6URK4. RN.

Interactioni

Subunit structurei

Identified in the spliceosome C complex.By similarity

Protein-protein interaction databases

BioGridi263224. 1 interaction.
STRINGi10116.ENSRNOP00000041984.

Structurei

3D structure databases

ProteinModelPortaliQ6URK4.
SMRiQ6URK4. Positions 29-210.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 11884RRM 1PROSITE-ProRule annotationAdd
BLAST
Domaini126 – 20580RRM 2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi211 – 379169Gly-richAdd
BLAST

Sequence similaritiesi

Contains 2 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0118. Eukaryota.
COG0724. LUCA.
GeneTreeiENSGT00760000118873.
HOGENOMiHOG000234442.
HOVERGENiHBG002295.
InParanoidiQ6URK4.
KOiK12741.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 2 hits.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6URK4-1) [UniParc]FASTAAdd to basket

Also known as: a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEVKPPPGRP QPDSGRRRRR RGEEGHDPKE PEQLRKLFIG GLSFETTDDS
60 70 80 90 100
LREHFEKWGT LTDCVVMRDP QTKRSRGFGF VTYSCVEEVD AAMCARPHKV
110 120 130 140 150
DGRVVEPKRA VSREDSVKPG AHLTVKKIFV GGIKEDTEEY NLRDYFEKYG
160 170 180 190 200
KIETIEVMED RQSGKKRGFA FVTFDDHDTV DKIVVQKYHT INGHNCEVKK
210 220 230 240 250
ALSKQEMQSA GSQRGRGGGS GNFMGRGGNF GGGGGNFGRG GNFGGRGGYG
260 270 280 290 300
GGGGGSRGSY GGGDGGYNGF GGDGGNYGGG PGYSSRGGYG GGGPGYGNQG
310 320 330 340 350
GGYGGGGGGY DGYNEGGNFG GGNYGGGGNY NDFGNYSGQQ QSNYGPMKGG
360 370
SFGGRSSGSP YGGGYGSGGG SGGYGSRRF
Length:379
Mass (Da):39,652
Last modified:July 5, 2004 - v1
Checksum:iD83C400A2B096E9B
GO
Isoform 2 (identifier: Q6URK4-2) [UniParc]FASTAAdd to basket

Also known as: b

The sequence of this isoform differs from the canonical sequence as follows:
     1-23: MEVKPPPGRPQPDSGRRRRRRGE → M

Show »
Length:357
Mass (Da):37,086
Checksum:i222DBB3DF00D2D7A
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2323MEVKP…RRRGE → M in isoform 2. 1 PublicationVSP_011400Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY363226 mRNA. Translation: AAQ63630.1.
AY363227 mRNA. Translation: AAQ63631.1.
BC081878 mRNA. Translation: AAH81878.1.
RefSeqiNP_001104764.1. NM_001111294.1. [Q6URK4-1]
NP_001104765.1. NM_001111295.1. [Q6URK4-2]
NP_937765.1. NM_198132.3. [Q6URK4-1]
XP_008760133.1. XM_008761911.1. [Q6URK4-2]
XP_008760134.1. XM_008761912.1. [Q6URK4-2]
UniGeneiRn.107690.
Rn.234205.
Rn.99947.

Genome annotation databases

EnsembliENSRNOT00000078872; ENSRNOP00000069876; ENSRNOG00000052968. [Q6URK4-1]
GeneIDi362152.
KEGGirno:362152.
UCSCiRGD:727807. rat. [Q6URK4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY363226 mRNA. Translation: AAQ63630.1.
AY363227 mRNA. Translation: AAQ63631.1.
BC081878 mRNA. Translation: AAH81878.1.
RefSeqiNP_001104764.1. NM_001111294.1. [Q6URK4-1]
NP_001104765.1. NM_001111295.1. [Q6URK4-2]
NP_937765.1. NM_198132.3. [Q6URK4-1]
XP_008760133.1. XM_008761911.1. [Q6URK4-2]
XP_008760134.1. XM_008761912.1. [Q6URK4-2]
UniGeneiRn.107690.
Rn.234205.
Rn.99947.

3D structure databases

ProteinModelPortaliQ6URK4.
SMRiQ6URK4. Positions 29-210.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi263224. 1 interaction.
STRINGi10116.ENSRNOP00000041984.

PTM databases

iPTMnetiQ6URK4.
PhosphoSiteiQ6URK4.

Proteomic databases

PaxDbiQ6URK4.
PRIDEiQ6URK4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000078872; ENSRNOP00000069876; ENSRNOG00000052968. [Q6URK4-1]
GeneIDi362152.
KEGGirno:362152.
UCSCiRGD:727807. rat. [Q6URK4-1]

Organism-specific databases

CTDi220988.
RGDi727807. Hnrnpa3.

Phylogenomic databases

eggNOGiKOG0118. Eukaryota.
COG0724. LUCA.
GeneTreeiENSGT00760000118873.
HOGENOMiHOG000234442.
HOVERGENiHBG002295.
InParanoidiQ6URK4.
KOiK12741.

Enzyme and pathway databases

ReactomeiR-RNO-72163. mRNA Splicing - Major Pathway.
R-RNO-72203. Processing of Capped Intron-Containing Pre-mRNA.

Miscellaneous databases

NextBioi678824.
PROiQ6URK4.

Gene expression databases

GenevisibleiQ6URK4. RN.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 2 hits.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Heterogeneous nuclear ribonucleoprotein A3, a novel RNA trafficking response element-binding protein."
    Ma A.S.W., Moran-Jones K., Shan J., Munro T.P., Snee M.J., Hoek K.S., Smith R.
    J. Biol. Chem. 277:18010-18020(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, PROTEIN SEQUENCE OF 114-126, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Wistar.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Kidney.
  3. "Phosphoproteomic analysis of rat liver by high capacity IMAC and LC-MS/MS."
    Moser K., White F.M.
    J. Proteome Res. 5:98-104(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  4. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-359, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  5. "Arginine methylation of hnRNP A2 does not directly govern its subcellular localization."
    Friend L.R., Landsberg M.J., Nouwens A.S., Wei Y., Rothnagel J.A., Smith R.
    PLoS ONE 8:E75669-E75669(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: METHYLATION AT ARG-214; ARG-216; ARG-226; ARG-239; ARG-246 AND ARG-286.

Entry informationi

Entry nameiROA3_RAT
AccessioniPrimary (citable) accession number: Q6URK4
Secondary accession number(s): Q6URK3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: July 5, 2004
Last modified: May 11, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.