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Protein

Phospholipid-transporting ATPase IK

Gene

Atp8b3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

P4-ATPase flippase which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules. May be responsible for the maintenance of asymmetric distribution of phosphatidylserine (PS) in spermatozoa membranes. Involved in acrosome reactions and binding of spermatozoa to zona pellucida.1 Publication

Catalytic activityi

ATP + H2O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei407 – 40714-aspartylphosphate intermediateBy similarity
Metal bindingi857 – 8571MagnesiumBy similarity
Metal bindingi861 – 8611MagnesiumBy similarity

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • magnesium ion binding Source: InterPro
  • phospholipid-translocating ATPase activity Source: MGI

GO - Biological processi

  • binding of sperm to zona pellucida Source: MGI
  • phospholipid translocation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.3.1. 3474.

Chemistry

SwissLipidsiSLP:000000353.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipid-transporting ATPase IK (EC:3.6.3.1)
Alternative name(s):
ATPase, class I, type 8B, member 3
Sperm aminophospholipid transporter
Short name:
SAPLT
Gene namesi
Name:Atp8b3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1914581. Atp8b3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7474CytoplasmicSequence analysisAdd
BLAST
Transmembranei75 – 9521HelicalSequence analysisAdd
BLAST
Topological domaini96 – 295200Exoplasmic loopSequence analysisAdd
BLAST
Transmembranei296 – 31621HelicalSequence analysisAdd
BLAST
Topological domaini317 – 33923CytoplasmicSequence analysisAdd
BLAST
Transmembranei340 – 36021HelicalSequence analysisAdd
BLAST
Topological domaini361 – 917557Exoplasmic loopSequence analysisAdd
BLAST
Transmembranei918 – 93821HelicalSequence analysisAdd
BLAST
Topological domaini939 – 9468CytoplasmicSequence analysis
Transmembranei947 – 96721HelicalSequence analysisAdd
BLAST
Topological domaini968 – 99528Exoplasmic loopSequence analysisAdd
BLAST
Transmembranei996 – 101621HelicalSequence analysisAdd
BLAST
Topological domaini1017 – 103317CytoplasmicSequence analysisAdd
BLAST
Transmembranei1034 – 105421HelicalSequence analysisAdd
BLAST
Topological domaini1055 – 10551Exoplasmic loopSequence analysis
Transmembranei1056 – 107621HelicalSequence analysisAdd
BLAST
Topological domaini1077 – 110428CytoplasmicSequence analysisAdd
BLAST
Transmembranei1105 – 112521HelicalSequence analysisAdd
BLAST
Topological domaini1126 – 1335210Exoplasmic loopSequence analysisAdd
BLAST

GO - Cellular componenti

  • acrosomal membrane Source: UniProtKB-SubCell
  • acrosomal vesicle Source: MGI
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Disrupted normal fertilization by sperm; sensitivity to sperm concentration and the time required to fertilize an egg is increased; the number of spermatozoa bound to zona pellucida is decreased.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13351335Phospholipid-transporting ATPase IKPRO_0000429839Add
BLAST

Proteomic databases

PaxDbiQ6UQ17.
PRIDEiQ6UQ17.

PTM databases

iPTMnetiQ6UQ17.

Expressioni

Tissue specificityi

Expressed in testis, specifically in spermatids within seminiferous tubules (at protein level).2 Publications

Developmental stagei

Developmentally regulated in testis. Expression is first detected at 17 days after birth and is later up-regulated up to adulthood amd maintained thereafter.1 Publication

Gene expression databases

GenevisibleiQ6UQ17. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000020383.

Structurei

3D structure databases

ProteinModelPortaliQ6UQ17.
SMRiQ6UQ17. Positions 137-701.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0206. Eukaryota.
COG0474. LUCA.
GeneTreeiENSGT00840000129828.
HOGENOMiHOG000202528.
HOVERGENiHBG050601.
InParanoidiQ6UQ17.
KOiK01530.
OMAiRRLSLMW.
OrthoDBiEOG7RRF68.
PhylomeDBiQ6UQ17.
TreeFamiTF300654.

Family and domain databases

Gene3Di2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR030349. ATP8B3.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006539. P-type_ATPase_IV.
IPR032631. P-type_ATPase_N.
IPR001757. P_typ_ATPase.
IPR032630. P_typ_ATPase_c.
[Graphical view]
PANTHERiPTHR24092. PTHR24092. 2 hits.
PTHR24092:SF78. PTHR24092:SF78. 2 hits.
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF16212. PhoLip_ATPase_C. 1 hit.
PF16209. PhoLip_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01652. ATPase-Plipid. 1 hit.
TIGR01494. ATPase_P-type. 1 hit.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6UQ17-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDGVHLGENL EDKDTEFTWE VKANDRTYHK QFKKKGFLCW RQKKYKSNAI
60 70 80 90 100
HTAKYNIFSF LPLNLYEQFH RMSNLYFLFI IILQGIPEIS TLPWFTLFAP
110 120 130 140 150
LVCLFVIRAT RDLVDDIGRH RSDKIINNRP CQILRGKSFL WKKWKNLCVG
160 170 180 190 200
DVVCLSKDSI VPADLLLLAS TEPSSLCYVE TADIDGETNL KFRQALTVTH
210 220 230 240 250
HELTSPKKMA SFQGTVTCEE PNSRMHHFVG SLEWNSRKYP LDIGNLLLRG
260 270 280 290 300
CKIRNTDTCY GLVIYAGLDT KIMKNCGKIH LKRTKLDLMM NKLVALIFLS
310 320 330 340 350
LVIASLLLTV GFTFMVKQFK AKHYYMSPTH GRSDAMESFF IFWGFLILLS
360 370 380 390 400
VMVPMAMFII AEFIYLGNSI FINWDLNMYY EPLDMPAKAR STSLNDQLGQ
410 420 430 440 450
VQYIFSDKTG TLTQNIMTFK KCCINGCIYD SDDEHGTLRK RNPYAWNPFA
460 470 480 490 500
DGKLQFYNKE LESLVQGRQD RAVQEFWRLL AICHTVMVQE KDNQLLYQAA
510 520 530 540 550
SPDEEALVTA ARNFGYVFLS RTQDTITLVE LGEERVYQVL AMMDFNSVRK
560 570 580 590 600
RMSVLVRNPE GSICLYTKGA DTVILERLRS KGVMEATTEE VLAAFAEQTL
610 620 630 640 650
RTLCLAYKDV EEDAYKEWEP EHQEAALLLQ NRAQALHQVY NKMEQNLQLL
660 670 680 690 700
GATAIEDKLQ DGVPETIKCL KKGNIKIWVL TGDKPETAVN IGFACQLLSE
710 720 730 740 750
NMIILEDKDI NQVLERYWED NVHQKAFKMM THHNMALVIN GEFLDQLLLS
760 770 780 790 800
LRKEPRALVQ NAVVDEVAQE PVVSALDFLQ KRRISQMWRN AGPSLGTSHS
810 820 830 840 850
ADSKIRESPE VQRERAFVDL ASKCQAVICC RVTPKQKALV VALVKKYQQV
860 870 880 890 900
VTLAIGDGAN DVNMIKTADI GVGLAGQEGM QAVQNSDYVL AQFCYLQRLL
910 920 930 940 950
LVHGRWSYMR VCKFLRYFFY KTVASMMAQI WFSLVNGFSA QPLYEGWFLA
960 970 980 990 1000
LFNLLYSTLP VLYIGLFEQD VTAEKSLKMP ELYMAGQKGE LFNYSIFMQA
1010 1020 1030 1040 1050
ITHGTITSMI NFFVTVMVSS DMSKAGSSHD YQSLGVLVAI SSLLSVTLEV
1060 1070 1080 1090 1100
MLVVKYWTLL FVGAVVLSLS SYVLMTSLTQ SLWMYRISPK TFPFLFADYN
1110 1120 1130 1140 1150
VLFEPCSLLL IVLNVALNVL PMLALRTIHR TVLKQRPKGE EEAPSEEVAV
1160 1170 1180 1190 1200
EPAMRHLRRG IPARRSSYAF SHREGYANLI TQGTILRRQT HVDDSDGGTV
1210 1220 1230 1240 1250
CESLNPPEED IPLQNKDSVF NPRKISILAK KRRHFFGKGS QEEVHPNTSS
1260 1270 1280 1290 1300
QTMEKQPTIH RDSETQKLPT TTSATSGKLL PSASEDEAFY SVASQYTLAS
1310 1320 1330
QPKHTDVHSS FWKSPLWRDS ASSSPSQLEV PRKQS
Length:1,335
Mass (Da):151,955
Last modified:July 5, 2004 - v1
Checksum:i7674AA921B28EE35
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY364445 mRNA. Translation: AAR12913.1.
AC152058 Genomic DNA. No translation available.
BC118977 mRNA. Translation: AAI18978.1.
CCDSiCCDS24024.1.
RefSeqiNP_080370.2. NM_026094.3.
UniGeneiMm.52511.

Genome annotation databases

EnsembliENSMUST00000020383; ENSMUSP00000020383; ENSMUSG00000003341.
GeneIDi67331.
KEGGimmu:67331.
UCSCiuc007gdo.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY364445 mRNA. Translation: AAR12913.1.
AC152058 Genomic DNA. No translation available.
BC118977 mRNA. Translation: AAI18978.1.
CCDSiCCDS24024.1.
RefSeqiNP_080370.2. NM_026094.3.
UniGeneiMm.52511.

3D structure databases

ProteinModelPortaliQ6UQ17.
SMRiQ6UQ17. Positions 137-701.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000020383.

Chemistry

SwissLipidsiSLP:000000353.

PTM databases

iPTMnetiQ6UQ17.

Proteomic databases

PaxDbiQ6UQ17.
PRIDEiQ6UQ17.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020383; ENSMUSP00000020383; ENSMUSG00000003341.
GeneIDi67331.
KEGGimmu:67331.
UCSCiuc007gdo.1. mouse.

Organism-specific databases

CTDi148229.
MGIiMGI:1914581. Atp8b3.

Phylogenomic databases

eggNOGiKOG0206. Eukaryota.
COG0474. LUCA.
GeneTreeiENSGT00840000129828.
HOGENOMiHOG000202528.
HOVERGENiHBG050601.
InParanoidiQ6UQ17.
KOiK01530.
OMAiRRLSLMW.
OrthoDBiEOG7RRF68.
PhylomeDBiQ6UQ17.
TreeFamiTF300654.

Enzyme and pathway databases

BRENDAi3.6.3.1. 3474.

Miscellaneous databases

NextBioi324260.
PROiQ6UQ17.
SOURCEiSearch...

Gene expression databases

GenevisibleiQ6UQ17. MM.

Family and domain databases

Gene3Di2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR030349. ATP8B3.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006539. P-type_ATPase_IV.
IPR032631. P-type_ATPase_N.
IPR001757. P_typ_ATPase.
IPR032630. P_typ_ATPase_c.
[Graphical view]
PANTHERiPTHR24092. PTHR24092. 2 hits.
PTHR24092:SF78. PTHR24092:SF78. 2 hits.
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF16212. PhoLip_ATPase_C. 1 hit.
PF16209. PhoLip_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01652. ATPase-Plipid. 1 hit.
TIGR01494. ATPase_P-type. 1 hit.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel aminophospholipid transporter exclusively expressed in spermatozoa is required for membrane lipid asymmetry and normal fertilization."
    Wang L., Beserra C., Garbers D.L.
    Dev. Biol. 267:203-215(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
    Strain: ICR.
    Tissue: Testis.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Expression of Atp8b3 in murine testis and its characterization as a testis specific P-type ATPase."
    Gong E.Y., Park E., Lee H.J., Lee K.
    Reproduction 137:345-351(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ATPASE ACTIVITY, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiAT8B3_MOUSE
AccessioniPrimary (citable) accession number: Q6UQ17
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 9, 2014
Last sequence update: July 5, 2004
Last modified: May 11, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.