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Protein

Protein amnionless

Gene

AMN

Organism
Canis familiaris (Dog) (Canis lupus familiaris)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Necessary for efficient absorption of vitamin B12. May direct the production of trunk mesoderm during development by modulating a bone morphogenetic protein (BMP) signaling pathway in the underlying visceral endoderm (By similarity).By similarity

GO - Biological processi

  1. multicellular organismal development Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Names & Taxonomyi

Protein namesi
Recommended name:
Protein amnionless
Gene namesi
Name:AMN
OrganismiCanis familiaris (Dog) (Canis lupus familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
ProteomesiUP000002254: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 366347ExtracellularSequence AnalysisAdd
BLAST
Transmembranei367 – 38721HelicalSequence AnalysisAdd
BLAST
Topological domaini388 – 45871CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Involvement in diseasei

Defects in AMN could be a cause of a canine form of megaloblastic anemia 1 (MGA1).

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 458439Protein amnionlessPRO_0000020701Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi35 – 351N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ6UKI2.

Interactioni

Subunit structurei

Interacts with CUBN/cubilin.By similarity

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini203 – 25452VWFCAdd
BLAST

Sequence similaritiesi

Contains 1 VWFC domain.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG037321.
InParanoidiQ6UKI2.
KOiK18259.

Family and domain databases

InterProiIPR026112. AMN.
[Graphical view]
PANTHERiPTHR14995. PTHR14995. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6UKI2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGALGRALLW LQLCALARAA YKLWVPTTDF EAAANWSQNR TPCAGAVVQF
60 70 80 90 100
PADKAVSVVV RASHGFSDML LPRDGEFVLA SGAGFGAADA GRDPDCGAGA
110 120 130 140 150
PALFLDPDRF SWHDPRLWRS GDAARGLFSV DAERVPCRHD DVVFPPDASF
160 170 180 190 200
RVGLGPGARP ARVRSVQVLG QTFTRDEDLA AFLASRAGRL RFHGPGALRV
210 220 230 240 250
GPGACADPSG CVCGDAEVQP WICAALLQPL GGRCPPAACP DALRPEGQCC
260 270 280 290 300
DLCGAIVSLT HGPTFDIERY RARLLRAFLP QYPGLQAAVS KVRRRPGPHT
310 320 330 340 350
EVQVVLAETG PQPGGAGRLA RALLADVAEH GEALGVLSAT ARESGAPVGD
360 370 380 390 400
GSAAGPLGSG SRAGLAGGVA AGLLLLLLAL AAGLLLLRRA PRLRWTKRER
410 420 430 440 450
LVATPVEAPL GFSNPVFDVA GSVGPVPRTP QPPPAQQAGS SSTSRSYFVN

PLFAEAEA
Length:458
Mass (Da):47,618
Last modified:July 5, 2004 - v1
Checksum:i4927E33B466B8D5A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY368152 mRNA. Translation: AAR13399.1.
RefSeqiNP_001002960.1. NM_001002960.1.
UniGeneiCfa.33575.

Genome annotation databases

GeneIDi403434.
KEGGicfa:403434.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY368152 mRNA. Translation: AAR13399.1.
RefSeqiNP_001002960.1. NM_001002960.1.
UniGeneiCfa.33575.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ6UKI2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi403434.
KEGGicfa:403434.

Organism-specific databases

CTDi81693.

Phylogenomic databases

HOVERGENiHBG037321.
InParanoidiQ6UKI2.
KOiK18259.

Miscellaneous databases

NextBioi20816952.

Family and domain databases

InterProiIPR026112. AMN.
[Graphical view]
PANTHERiPTHR14995. PTHR14995. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The functional cobalamin (vitamin B12)-intrinsic factor receptor is a novel complex of cubilin and amnionless."
    Fyfe J.C., Madsen M., Hoejrup P., Christensen E.I., Tanner S.M., de la Chapelle A., He Q., Moestrup S.K.
    Blood 103:1573-1579(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Canine Imerslund-Grasbeck syndrome maps to a region orthologous to HSA14q."
    He Q., Fyfe J.C., Schaeffer A.A., Kilkenney A., Werner P., Kirkness E.F., Henthorn P.S.
    Mamm. Genome 14:758-764(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: POSSIBLE INVOLVEMENT IN CANINE MGA1.

Entry informationi

Entry nameiAMNLS_CANFA
AccessioniPrimary (citable) accession number: Q6UKI2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: July 5, 2004
Last modified: January 7, 2015
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.