Q6UKI2 (AMNLS_CANFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein amnionless

Gene names

Name:

AMN

Organism

Canis familiaris (Dog) (Canis lupus familiaris) [Reference proteome]

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis › Canis lupus

Protein attributes

Sequence length	458 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Necessary for efficient absorption of vitamin B12. May direct the production of trunk mesoderm during development by modulating a bone morphogenetic protein (BMP) signaling pathway in the underlying visceral endoderm By similarity.
Subunit structure	Interacts with CUBN/cubilin By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein Potential.
Involvement in disease	Defects in AMN could be a cause of a canine form of megaloblastic anemia 1 (MGA1). Ref.2
Sequence similarities	Contains 1 VWFC domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Developmental protein
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 458	439	Protein amnionless		PRO_0000020701
Regions
	Topological domain	20 – 366	347	Extracellular Potential
	Transmembrane	367 – 387	21	Helical; Potential
	Topological domain	388 – 458	71	Cytoplasmic Potential
	Domain	203 – 254	52	VWFC
Amino acid modifications
	Glycosylation	35	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6UKI2 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 4927E33B466B8D5A	FASTA	458	47,618
	10 20 30 40 50 60 MGALGRALLW LQLCALARAA YKLWVPTTDF EAAANWSQNR TPCAGAVVQF PADKAVSVVV 70 80 90 100 110 120 RASHGFSDML LPRDGEFVLA SGAGFGAADA GRDPDCGAGA PALFLDPDRF SWHDPRLWRS 130 140 150 160 170 180 GDAARGLFSV DAERVPCRHD DVVFPPDASF RVGLGPGARP ARVRSVQVLG QTFTRDEDLA 190 200 210 220 230 240 AFLASRAGRL RFHGPGALRV GPGACADPSG CVCGDAEVQP WICAALLQPL GGRCPPAACP 250 260 270 280 290 300 DALRPEGQCC DLCGAIVSLT HGPTFDIERY RARLLRAFLP QYPGLQAAVS KVRRRPGPHT 310 320 330 340 350 360 EVQVVLAETG PQPGGAGRLA RALLADVAEH GEALGVLSAT ARESGAPVGD GSAAGPLGSG 370 380 390 400 410 420 SRAGLAGGVA AGLLLLLLAL AAGLLLLRRA PRLRWTKRER LVATPVEAPL GFSNPVFDVA 430 440 450 GSVGPVPRTP QPPPAQQAGS SSTSRSYFVN PLFAEAEA

References

[1]	"The functional cobalamin (vitamin B12)-intrinsic factor receptor is a novel complex of cubilin and amnionless." Fyfe J.C., Madsen M., Hoejrup P., Christensen E.I., Tanner S.M., de la Chapelle A., He Q., Moestrup S.K. Blood 103:1573-1579(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Canine Imerslund-Grasbeck syndrome maps to a region orthologous to HSA14q." He Q., Fyfe J.C., Schaeffer A.A., Kilkenney A., Werner P., Kirkness E.F., Henthorn P.S. Mamm. Genome 14:758-764(2003) [PubMed] [Europe PMC] [Abstract] Cited for: POSSIBLE INVOLVEMENT IN CANINE MGA1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY368152 mRNA. Translation: AAR13399.1.
RefSeq	NP_001002960.1. NM_001002960.1.
UniGene	Cfa.33575.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	403434.
KEGG	cfa:403434.
Organism-specific databases
CTD	81693.
Phylogenomic databases
HOVERGEN	HBG037321.
Family and domain databases
InterPro	IPR026112. AMN. [Graphical view]
PANTHER	PTHR14995. PTHR14995. 1 hit.
PROSITE	PS01208. VWFC_1. False negative. PS50184. VWFC_2. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	20816952.

Entry information

Entry name

AMNLS_CANFA

Accession

Primary (citable) accession number: Q6UKI2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	July 5, 2004
Last modified:	October 3, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program