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Q6UKI2 (AMNLS_CANFA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein amnionless
Gene names
Name:AMN
OrganismCanis familiaris (Dog) (Canis lupus familiaris) [Reference proteome]
Taxonomic identifier9615 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis

Protein attributes

Sequence length458 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Necessary for efficient absorption of vitamin B12. May direct the production of trunk mesoderm during development by modulating a bone morphogenetic protein (BMP) signaling pathway in the underlying visceral endoderm By similarity.

Subunit structure

Interacts with CUBN/cubilin By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein Potential.

Involvement in disease

Defects in AMN could be a cause of a canine form of megaloblastic anemia 1 (MGA1). Ref.2

Sequence similarities

Contains 1 VWFC domain.

Ontologies

Keywords
   Cellular componentMembrane
   DomainSignal
Transmembrane
Transmembrane helix
   Molecular functionDevelopmental protein
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmulticellular organismal development

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 458439Protein amnionless
PRO_0000020701

Regions

Topological domain20 – 366347Extracellular Potential
Transmembrane367 – 38721Helical; Potential
Topological domain388 – 45871Cytoplasmic Potential
Domain203 – 25452VWFC

Amino acid modifications

Glycosylation351N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q6UKI2 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 4927E33B466B8D5A

FASTA45847,618
        10         20         30         40         50         60 
MGALGRALLW LQLCALARAA YKLWVPTTDF EAAANWSQNR TPCAGAVVQF PADKAVSVVV 

        70         80         90        100        110        120 
RASHGFSDML LPRDGEFVLA SGAGFGAADA GRDPDCGAGA PALFLDPDRF SWHDPRLWRS 

       130        140        150        160        170        180 
GDAARGLFSV DAERVPCRHD DVVFPPDASF RVGLGPGARP ARVRSVQVLG QTFTRDEDLA 

       190        200        210        220        230        240 
AFLASRAGRL RFHGPGALRV GPGACADPSG CVCGDAEVQP WICAALLQPL GGRCPPAACP 

       250        260        270        280        290        300 
DALRPEGQCC DLCGAIVSLT HGPTFDIERY RARLLRAFLP QYPGLQAAVS KVRRRPGPHT 

       310        320        330        340        350        360 
EVQVVLAETG PQPGGAGRLA RALLADVAEH GEALGVLSAT ARESGAPVGD GSAAGPLGSG 

       370        380        390        400        410        420 
SRAGLAGGVA AGLLLLLLAL AAGLLLLRRA PRLRWTKRER LVATPVEAPL GFSNPVFDVA 

       430        440        450 
GSVGPVPRTP QPPPAQQAGS SSTSRSYFVN PLFAEAEA 

« Hide

References

[1]"The functional cobalamin (vitamin B12)-intrinsic factor receptor is a novel complex of cubilin and amnionless."
Fyfe J.C., Madsen M., Hoejrup P., Christensen E.I., Tanner S.M., de la Chapelle A., He Q., Moestrup S.K.
Blood 103:1573-1579(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Canine Imerslund-Grasbeck syndrome maps to a region orthologous to HSA14q."
He Q., Fyfe J.C., Schaeffer A.A., Kilkenney A., Werner P., Kirkness E.F., Henthorn P.S.
Mamm. Genome 14:758-764(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: POSSIBLE INVOLVEMENT IN CANINE MGA1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY368152 mRNA. Translation: AAR13399.1.
RefSeqNP_001002960.1. NM_001002960.1.
UniGeneCfa.33575.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ6UKI2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID403434.
KEGGcfa:403434.

Organism-specific databases

CTD81693.

Phylogenomic databases

HOVERGENHBG037321.

Family and domain databases

InterProIPR026112. AMN.
[Graphical view]
PANTHERPTHR14995. PTHR14995. 1 hit.
ProtoNetSearch...

Other

NextBio20816952.

Entry information

Entry nameAMNLS_CANFA
AccessionPrimary (citable) accession number: Q6UKI2
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: July 5, 2004
Last modified: February 19, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families